Swimming behaviour of silver carp (Hypophthalmichthys molitrix) in response to turbulent flow induced by a D-cylinder.

Turbulence is a complex hydraulic phenomenon which commonly occurs in natural streams and fishways. Riverine fish are subjected to heterogeneous flow velocities and turbulence, which may affect their movements and ability to pass the fishways. However, studies focusing on fish response to turbulent flows are lacking for many species. Here we investigate the effects of the turbulence created by a vertical half-cylinder of various diameters (1.9, 2.5, 3.2 and 5.0 cm) on the swimming ability and behaviour of silver carp, Hypophthalmichthys molitrix. The large D-cylinders (3.0 and 5.0 cm) create specific vorticity and reduced velocities areas in their vicinity, which favours flow refuging behaviours (FRBs) and thus increased relative critical swimming speeds (Urcrit , BL/s) of silver carp, by comparison to free-flow conditions and cylinders of smaller diameter (1.9 and 2.5 cm). The flow speed at which silver carp maximized FRBs such as Karman gaiting downstream of the cylinder, holding position in the bow wake or entraining on the side ranged from 40 to 70 cm s-1 , depending on fish body size. When holding station near a cylinder under optimal flow speeds, the distance between the fish and the cylinder is related to the size of the fish, but also to the size of the cylinder and the produced vortices. The optimal holding region in the drag wake of the cylinder ranged from 28 to 40 cm downstream of the centre of the cylinder, depending on the size of the fish. Smaller fish, however, tend to use the reduced velocities areas located in the bow wake of the large cylinders. We hypothesize that fish will display FRBs, including maintaining a Karman gait in turbulent flow, when the ratio of the cylinder diameter to their body length is between 1:3 and 1:4. They also match their tail beat frequency to the vortex shedding frequency of the cylinder. Our results provide a better understanding of how silver carp respond to turbulent flows around physical structures, with implications for the design of nature-like fishways or exclusion devices in both its native and invasive ranges.

Incorporating the life stages of fish into habitat assessment frameworks: A case study in the Baihetan Reservoir.

Although it is widely accepted that the construction of dams may alter fish habitats, few studies have followed the life cycles of fish and combined the environmental conditions with the ecological behaviors and habit preferences of fish during reproductive processes to assess its effects of dam construction. In this study, we call for more sophisticated and holistic assessment framework, including effectiveness of technologies intended to mitigate environmental impacts in different life stages. An assessment framework that considers the swimming ability, perception ability of water flow and environmental preference of different fish species during migration, spawning and hatching was proposed. We used the Baihetan Reservoir as an example environment to assess the impoundment effect on the habitat of a tributary upstream of the reservoir. We observed shifts in the habitats of target fish in different life stages which is dominated by reservoir operation of the Baihetan Dam. Combined with the response of fish activities to impoundment, the selection of suitable positions for artificial breeding and release projects and the outlet of the fish transportation system were recommended measures to improve the migration possibilities. Our reassessment results also demonstrated the theoretical possibility and feasibility of joint improvements in spawning and hatching periods using instream structures. Our framework provides a complete set of "assessment-solution" processes for developers and managers to address the aquatic ecological degradation caused by resource development, and its use is strongly recommended for assessments or assessments of damming effects in other regions and on other fish species.

Lyophilization enhances the stability of Panax notoginseng total saponins-loaded transfersomes without adverse effects on ex vivo/in vivo skin permeation.

Transfersomes (TFSs) have been extensively investigated to enhance transdermal drug delivery. As a colloidal dispersion system, TFSs are prone to problems such as particle aggregation and sedimentation, oxidation and decomposition of phospholipids. To enhance the stability of panax notoginseng saponins (PNS)-loaded transfersomes (PNS-TFSs) without adverse influences on their skin permeation, we prepared lyophilized PNS-loaded transfersomes (PNS-FD-TFSs), clarified their physicochemical characteristics and investigated their in vitro drug release, ex vivo skin permeation/deposition and in vivo pharmacokinetics. In this study, a simple, fast and controllable process was developed for preparing lyophilized PNS-TFSs. In the optimized PNS-FD-TFS formulation, sucrose and trehalose were added to the PNS-TFS dispersion with a mass ratio of trehalose, sucrose, and phospholipid of 3:2:1, and the mixture was frozen at -80 °C for 12 h followed by lyophilization at -45 °C and 5 Pa for 24 h. The optimized formulation of PNS-FD-TFSs was screened based on the appearance and reconstitution time of the lyophilized products, vesicle size, and PDI of the freshly reconstituted dispersions. It maintained stable physicochemical properties for at least 6 months at 4 °C. The vesicle size of PNS-FD-TFSs was below 100 nm and homogenous with a polydispersity index of 0.2 after reconstitution. The average encapsulation efficiencies of the five index saponins notoginsenoside R1 (NGR1), ginsenoside Rg1 (GRg1), ginsenoside Re (GRe), ginsenoside Rb1 (GRb1) and ginsenoside Rd (GRd) in PNS-FD-TFSs were 68.41 ± 5.77%, 68.95 ± 6.08%, 65.46 ± 10.95%, 91.50 ± 5.62% and 95.78 ± 1.70%, respectively. The reconstituted dispersions of PNS-FD-TFSs were similar to PNS-TFSs in in vitro release, ex vivo skin permeation, and deposition. The pharmacokinetic studies showed that, compared with the PNS liposomes (PNS-LPS), the PNS-FD-TFS-loaded drug could permeate through the skin and enter the blood rapidly. It can be concluded that the lyophilization process can effectively improve the stability of PNS-TFSs without compromising their transdermal absorption properties.

Correlation between in vivo microdialysis pharmacokinetics and ex vivo permeation for sinomenine hydrochloride transfersomes with enhanced skin absorption.

Transdermal drug delivery systems have drawn increasing attention in recent decades. Estimation of the correlation between ex vivo permeation and in vivo absorption (EVIVC) is an indispensable issue in the research and development of transdermal pharmaceutical products. In this paper, sinomenine hydrochloride (SH) transfersomes (SHTs) were prepared with sodium deoxycholate as edge activator, while SH liposomes (SHLs) were prepared as a control preparation. The transdermal permeation characteristics differences between them were explored by an ex vivo skin permeation experiment with Franz diffusion cell and an in vivo skin/blood pharmacokinetic experiment facilitated by double-sited microdialysis sampling technique. The curves of percentage absorbed versus time (absorption curves) under the skin and in the blood were plotted according to the percentages calculated by the deconvolution approach with the application of Wagner-Nelson model, and were correlated with the ex vivo permeation curves to evaluate a level A correlation, while a level C correlation evaluation was conducted based on the in vivo steady-state blood concentration (Css) and the ex vivo steady-state transdermal permeation rate. The ex vivo permeation test indicated that the cumulative transdermal permeated amount of SH at 36 h in SHTs was about 1.7 times of that in SHLs. The skin pharmacokinetic data showed that the Css and AUC0-t of SHTs were about 8.8 and 8.0 times of those of SHLs, respectively, and the MRT0-t of SHTs was shorter. The blood pharmacokinetic data showed that the Css and AUC0-t of SHTs were about 3.7 and 2.9 times of those of SHLs, respectively. The in vivo absorption curves were correlated well with the ex vivo permeation curves. The squares of correlation coefficient (R2) for SHTs and SHLs were 0.9153 and 0.9355 respectively in the skin, were 0.8536 and 0.7747 respectively in the blood. As to level C EVIVC, there was no significant difference between the predicted Css from ex vivo and the measured Cssin vivo. The transfersomes can be employed as effective vehicles to promote the transdermal absorption of SH, and it is feasible to predict the in vivo skin/blood pharmacokinetic properties of SHLs and SHTs based on the ex vivo skin permeation characteristics.

Impact of Low-Head Dam Removal on River Morphology and Habitat Suitability in Mountainous Rivers.

Dam removal is considered an effective measure to solve the adverse ecological effects caused by dam construction and has started to be considered in China. The sediment migration and habitat restoration of river ecosystems after dam removal have been extensively studied abroad but are still in the exploratory stage in China. However, there are few studies on the ecological response of fishes at different growth stages. Considering the different habitat preferences of Schizothorax prenanti (S. prenanti) in the spawning and juvenile periods, this study coupled field survey data and a two-dimensional hydrodynamic model to explore the changes in river morphology at different scales and the impact of changes in hydrodynamic conditions on fish habitat suitability in the short term. The results show that after the dam is removed, in the upstream of the dam, the riverbed is eroded and cut down and the riverbed material coarsens. With the increase in flow velocity and the decrease in flow area, the weighted usable area (WUA) in the spawning and juvenile periods decreases by 5.52% and 16.36%, respectively. In the downstream of the dam, the riverbed is markedly silted and the bottom material becomes fine. With the increase in water depth and flow velocity, the WUA increases by 79.91% in the spawning period and decreases by 67.90% in the juvenile period, which is conducive to adult fish spawning but not to juvenile fish growth. The changes in physical habitat structure over a short time period caused by dam removal have different effects on different fish development periods, which are not all positive. The restoration of stream continuity increases adult fish spawning potential while limiting juvenile growth. Thus, although fish can spawn successfully, self-recruitment of fish stocks can still be affected if juvenile fish do not grow successfully. This study provides a research basis for habitat assessment after dam removal and a new perspective for the subsequent adaptive management strategy of the project.

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To investigate the swimming ability of two Schizothorax species in the Yalung River and provide basic parameters for the studies on fish behavior and the design of fish passage, we exa-mined the induced velocity, critical swimming speed, and burst swimming speed in Schizothorax dolichonema and Schizothorax prenanti with incremental velocity method and the durable swimming speed in S. dolichonema with fixed velocity method. The results showed that the induced velocity of both species increased first and then plateaued with the increases of body length, with the maximum values being lower than 0.2 m·s-1. The critical swimming speed and burst swimming speed of S. dolichonema were (0.81±0.20) and (1.49±0.26) m·s-1, respectively, while the relative critical swimming speed and the relative burst swimming speed were (4.90±1.73) and (9.77±1.72) BL·s-1 (BL: body length), respectively. For S. prenanti, the critical swimming speed and burst swimming speed were (0.73±0.24) and (1.17±0.39) m·s-1, respectively, while the relative critical swimming speed was (6.88±2.82) BL·s-1, and the relative burst swimming speed was (11.75±2.77) BL·s-1. The swimming duration of S. dolichonema was negatively correlated with the flow velocity of 0.7-1.5 m·s-1, and the relationship between fatigue time (T) and flow velocity (V) was fitted into lgT=-2.52V+5.59. The relationship between expected fishway length (d) and the tolerable maximum average flow velocity (Vf max) was accordingly derived to be Vf max=-0.17lnd+1.74. Taken together, the fishway targeting S. dolichonema and S. prenanti was recommended to generate the in-channel velocity larger than 0.2 m·s-1, while the velocity at the entrance and verticle slot should be 0.73-1.67 m·s-1, and the main-flow velocity in rest pools should be 0.2-0.7 m·s-1.