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1.
Medicina (Kaunas) ; 57(11)2021 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-34833491

RESUMO

Background and Objectives: Due to the unexpected spread of coronavirus disease 2019 (COVID-19), there was a serious crisis of emergency medical system collapse. Healthcare workers working in the emergency department were faced with psychosocial stress and workload changes. Materials and Methods: This was a cross-sectional survey of healthcare workers in the emergency department in Daegu and Gyeongbuk, Korea, from November 16 to 25, 2020. In the survey, we assessed the general characteristics of the respondents; changes in the working conditions before and after the COVID-19 pandemic; and resulting post-traumatic stress disorder, depression and anxiety statuses using 49 questions. Results: A total of 529 responses were collected, and 520 responses were included for the final analyses. Changes in working conditions and other factors due to COVID-19 varied by emergency department level, region and disease group. Working hours, intensity, role changes, depression and anxiety scores were higher in the higher level emergency department. Isolation ward insufficiency and the risk of infection felt by healthcare workers tended to increase in the lower level emergency department. Treatment and transfer delay were higher in the fever and respiratory disease groups (M = 3.58, SD = 1.18; M = 4.08, SD = 0.95), respectively. In all the disease groups, both treatment and transfer were delayed more in Gyeongbuk than in Daegu. Conclusions: Different goals should be pursued by the levels and region of the emergency department to overcome the effects of the COVID-19 pandemic and promote optimal care.


Assuntos
COVID-19 , Serviços Médicos de Emergência , Ansiedade , Estudos Transversais , Depressão/epidemiologia , Surtos de Doenças , Serviço Hospitalar de Emergência , Pessoal de Saúde , Humanos , Pandemias , SARS-CoV-2 , Estresse Psicológico/epidemiologia , Carga de Trabalho
2.
Virus Res ; 86(1-2): 21-31, 2002 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-12076826

RESUMO

The effectiveness of a cocktailed HSV-1 three-glycoprotein B, H, and L gene vaccine in comparison to individual glycoprotein gene vaccines was studied with regard to protecting against the HSV-1 infection. Three glycoprotein gene recombinant DNA vaccines, which produced the corresponding glycoproteins in Vero cells, were constructed using a CMV promoter. The cocktailed DNA vaccines were prepared by combining all three genes. The titers of neurtalizing antibody following the immunization of the five vaccines were KOS(1/1024)>B:H:L=B(1/512)>H:L(1/64)>H(1/16) genes. The mice, which were immunized with L gene alone failed to induce enough neutralizing antibody. The CTL activity was rated as KOS (95%)>B:H:L (80%)>B(60%)>H:L(50%)> H (35%) gene vaccines at an E:T ratio of 50:1. The H gene alone or L gene vaccine alone induced little CTL activity. The protection rates of the DNA-vaccinated mice against the lethal intraperitoneal (i.p.) or i.m challenges were shown as KOS>B:H:L>B>H:L>H gene vaccines, and the protection activity depended on the lethal dosage of the challenging virus, which are inversely proportional to each other. Compared with the mice, which were vaccinated with individual DNA vaccines, the mice, which were vaccinated with the cocktailed three-gene vaccine, were shown to be better protected against the lethal challenging doses. It can be concluded that vaccination with the cocktailed three gene vaccines is more effective in protecting mice from the viral challenge and the protection rate varies inversely with the amount of lethal challenging dose used, although all DNA vaccines failed to block the latent infection in sensory nerves.


Assuntos
Glicoproteínas/imunologia , Herpes Simples/prevenção & controle , Herpesvirus Humano 1/imunologia , Vacinas de DNA/administração & dosagem , Proteínas do Envelope Viral/imunologia , Vacinas Virais/uso terapêutico , Animais , Anticorpos Antivirais/biossíntese , Anticorpos Antivirais/sangue , Anticorpos Antivirais/imunologia , Western Blotting , Chlorocebus aethiops , Modelos Animais de Doenças , Glicoproteínas/genética , Camundongos , Camundongos Endogâmicos BALB C , Reação em Cadeia da Polimerase , Proteínas Recombinantes/imunologia , Células Vero , Proteínas do Envelope Viral/genética , Vacinas Virais/imunologia , Replicação Viral/efeitos dos fármacos
3.
DNA Seq ; 13(4): 225-9, 2002 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-12487026

RESUMO

A EED pseudogene transcript (psXEED) has been isolated from the cDNA pool of Xenopus laevis. The open reading frame of psXEED was interrupted with a 40 bp insertion. Its untranslated region was identical with that of the functional XEED. Furthermore, the EED pseudogene was organized as a processed pseudogene in the genomic level. Interestingly, the EED pseudogene was transcribed both maternally and zygotically throughout embryogenesis.


Assuntos
Pseudogenes , Proteínas Repressoras/genética , Proteínas de Xenopus , Xenopus/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , DNA Complementar , Embrião não Mamífero/metabolismo , Desenvolvimento Embrionário , Dados de Sequência Molecular , Proteínas do Grupo Polycomb , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Homologia de Sequência de Aminoácidos , Xenopus/embriologia
4.
Korean J Anesthesiol ; 67(1): 61-5, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25097742

RESUMO

We experienced a case of deep vein thrombosis after spine surgery in the prone position with a central venous catheter (CVC). Posterior lumbar interbody fusion was performed on a 73-year-old female patient who was diagnosed with spinal stenosis. Accordingly, in the operation room under general anesthesia, two-lumen CVC were inserted into the left subclavian vein. The surgery was performed in the prone position with a Wilson frame. On the next day, there was a sudden occurrence of severe edema in the patient's left arm. By ultrasonography and computed tomography scanning, extensive deep vein thrombosis was observed in the left subclavian vein. The existence of a factor affecting blood flow such as the prone position may increase the risk of thrombus formation. Therefore, careful perioperative evaluation should be implemented.

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