RESUMO
OBJECTIVE: To determine the role of PPE25 in the infection of M. smegmatis (MS) in polymorphonuclear neutrophils (PMNs). METHODS: In MS-ppe25 group, PPE25 was expressed in non-pathogenic fast-growing M. tuberculosis (Mtb) that infected PMNs. The empty vector MS (MS-vec group) was served as control. Their colony formation was observed, including the size and growth curves of single colonies. The colony forming unit (CFU) indicated bacterial vitality. The percentage of lactate dehydrogenase (LDH) release measured PMN death. The role of PPE25 protein in MS infections was analyzed by reactive oxygen species (ROS) detected by flow cytometry, nitric oxide (NO) level detected by nitrate reductase, cytokine interleukin (IL) and tumor necrosis factor-α (TNF-α) detected by ELISA. RESULTS: PPE25 protein had no effect on MS growth, colony formation and the size of single colonies. MS-infected PMN had higher percentages of CFU and LDH release 2, 6, and 12 h after infections compared with the MS-vec group (P<0.05). MS-infected PMN also had lower levels of ROS and NO levels 2 h after infections (P<0.01), consistently higher levels of TNF-α (P<0.01), and higher levels of IL-1ß infusion 6 h after MS infections (P<0.01). CONCLUSIONS: PPE25 protein increases the survival of MS in PMN, induces cell necrosis, inhibits the expressions of ROS and NO, and changes the secretion of cytokines, which helps spread of the pathogen by evading host immunity.
RESUMO
AIM: To observe the change and the clinical significance of S100ß protein level in cerebrospinal fluid and serum from the patients with cerebral hemorrhage (CH). METHODS: ELISA was used to detect the expression of S100ß protein in CSF and serum from CH patients control with Inguinal Hernia or great saphenous varix patients. Meanwhile, rabbit CH model at 6 h, 12 h, 24 h, 48 h, 72 h and 96 h . RESULTS: The levels of CSF S100ß protein at acute stage of CH patients increased significantly compared with those at recovery stage of CH patients and control group(P<0.01). The levels of S100ß protein in CSF from CH patients increased significantly compared with those in serum (P<0.01).The levels of S100ß protein in CSF of rabbit experimental group increased significantly compared with those of sham operation group at different time points(P<0.01). CONCLUSION: The level of S100ß protein in CSF from CH patients increases. It may be a biomarker as reflecting degree of pathogenetic and predicting outcome in the CH patients.