Biome boundary maintained by intense belowground resource competition in world's thinnest-rooted plant community.

Recent findings point to plant root traits as potentially important for shaping the boundaries of biomes and for maintaining the plant communities within. We examined two hypotheses: 1) Thin-rooted plant strategies might be favored in biomes with low soil resources; and 2) these strategies may act, along with fire, to maintain the sharp boundary between the Fynbos and Afrotemperate Forest biomes in South Africa. These biomes differ in biodiversity, plant traits, and physiognomy, yet exist as alternative stable states on the same geological substrate and in the same climate conditions. We conducted a 4-y field experiment to examine the ability of Forest species to invade the Fynbos as a function of growth-limiting nutrients and belowground plant-plant competition. Our results support both hypotheses: First, we found marked biome differences in root traits, with Fynbos species exhibiting the thinnest roots reported from any biome worldwide. Second, our field manipulation demonstrated that intense belowground competition inhibits the ability of Forest species to invade Fynbos. Nitrogen was unexpectedly the resource that determined competitive outcome, despite the long-standing expectation that Fynbos is severely phosphorus constrained. These findings identify a trait-by-resource feedback mechanism, in which most species possess adaptive traits that modify soil resources in favor of their own survival while deterring invading species. Our findings challenge the long-held notion that biome boundaries depend primarily on external abiotic constraints and, instead, identify an internal biotic mechanism-a selective feedback among traits, plant-plant competition, and ecosystem conditions-that, along with contrasting fire regime, can act to maintain biome boundaries.

Fire-modulated fluctuations in nutrient availability stimulate biome-scale floristic turnover in time, and elevated species richness, in low-nutrient fynbos heathland.

BACKGROUND AND AIMS: In many systems, postfire vegetation recovery is characterized by temporal changes in plant species composition and richness. We attribute this to changes in resource availability with time since fire, with the magnitude of species turnover determined by the degree of resource limitation. Here, we test the hypothesis that postfire species turnover in South African fynbos heathland is powered by fire-modulated changes in nutrient availability, with the magnitude of turnover in nutrient-constrained fynbos being greater than in fertile renosterveld shrubland. We also test the hypothesis that floristic overlaps between fynbos and renosterveld are attributable to nutritional augmentation of fynbos soils immediately after fire. METHODS: We use vegetation survey data from two sites on the Cape Peninsula to compare changes in species richness and composition with time since fire. KEY RESULTS: Fynbos communities display a clear decline in species richness with time since fire, whereas no such decline is apparent in renosterveld. In fynbos, declining species richness is associated with declines in the richness of plant families having high foliar concentrations of nitrogen, phosphorus and potassium and possessing attributes that are nutritionally costly. In contrast, families that dominate late-succession fynbos possess adaptations for the acquisition and retention of sparse nutrients. At the family level, recently burnt fynbos is compositionally more similar to renosterveld than is mature fynbos. CONCLUSIONS: Our data suggest that nutritionally driven species turnover contributes significantly to fynbos community richness. We propose that the extremely low baseline fertility of fynbos soils serves to lengthen the nutritional resource axis along which species can differentiate and coexist, thereby providing the opportunity for low-nutrient extremophiles to coexist spatially with species adapted to more fertile soil. This mechanism has the potential to operate in any resource-constrained system in which episodic disturbance affects resource availability.

Quantitative evaluation of the drivers of species richness in a Mediterranean ecosystem (Cape, South Africa).

BACKGROUND AND AIMS: Mediterranean ecosystems have a high vascular plant species richness relative to their surface area. This species richness (SR), representing the balance between speciation and extinction, has been attributed to multiple mechanisms that result in both high rates of speciation and/or low rates of extinction. An abiding question is, however, what is special about Mediterranean ecosystems that enables this high SR? Apart from long-term climatic stability of the region, SR has also been related to resource availability, the many individuals hypothesis, resource spatial heterogeneity, temporal heterogeneity and to biotic feedbacks. METHODS: Spatial patterns of species richness were related to climatic, edaphic, and biotic variables and spatial variability within the Greater Cape Floristic Region (GCFR) of South Africa. Boosted regression tree models were used to explore the strength of relationships between SR and environmental predictors related to each hypothesised mechanism. KEY RESULTS: Water availability (i.e., precipitation) was a stronger predictor of SR than potential evapotranspiration or temperature. Scarcity of nutrients was also related to SR. There was no indication that SR was related to the density of individuals and only temporal heterogeneity induced by fire was related to SR. Spatial heterogeneities of climatic, edaphic, and biotic variables were strongly associated with SR. Biotic interactions remain difficult to assess, although we have some evidence for a putative role in regulating SR. CONCLUSIONS: While the lack of ecosystem-resetting disturbances (e.g., glaciation) is undoubtedly a key requirement for high species accumulation, predictably, no one explanation holds the key to understanding SR. In the GCFR high SR is the product of a combination of adequate water, nutrient scarcity, spatial and temporal heterogeneity, and possibly biotic feedbacks.

Does a tradeoff between trait plasticity and resource conservatism contribute to the maintenance of alternative stable states?

Phenotypic plasticity facilitates species persistence across resource gradients but may be limited in low-resource environments requiring resource conservation. We investigated the tradeoff between trait plasticity and resource conservatism across a biome boundary characterized by high turnover in nutrient and light availability, and whether this contributes to the maintenance of alternative stable states. Differences in plasticity were determined by comparing species' leaf and foliar nutritional trait responses to light, represented by leaf area index (LAI), and soil nutrient availability across forest-shrubland boundaries in South Africa. Although forest had higher LAI and soil nutrient availability than shrubland, forest species experienced greater resource variation. With increasing LAI and nutrient availability, forest species increased their leaf size, specific leaf area and leaf area/stem length, and decreased their foliar [N] and [K]. Although these responses are indicative of plasticity, shrubland species appeared to lack plasticity as evidenced by limited trait variation with environmental heterogeneity. Inhabiting diverse light environments imposed by forests probably selects for plasticity, whereas light-saturated, fire-prone, nutrient-poor environments that select for conservative leaf traits and below-ground investments compromise plasticity in shrubland species. This pattern suggests a tradeoff between trait plasticity and resource conservatism, which may support the stability of alternative vegetation states.

Specialization to Extremely Low-Nutrient Soils Limits the Nutritional Adaptability of Plant Lineages.

Specialization to extreme selective situations promotes the acquisition of traits whose coadaptive integration may compromise evolutionary flexibility and adaptability. We test this idea in the context of the foliar stoichiometry of plants native to the South African Cape. Whereas foliar concentrations of nitrogen, phosphorus (P), potassium (K), calcium, magnesium, and sodium showed strong phylogenetic signal, as did the foliar ratios of these nutrients to P, the same was not true of the corresponding soil values. In addition, although foliar traits were often related to soil values, the coefficients of determination were consistently low. These results identify foliar stoichiometry as having a strong genetic component, with variation in foliar nutrient concentrations, especially [P] and [K], being identified as potentially adaptive. Comparison of stoichiometric variation across 11 similarly aged clades revealed consistently low foliar nutrient concentrations in lineages showing specialization to extremely low-nutrient fynbos heathlands. These lineages also display lower rates of evolution of these traits as well as a reduced tendency for foliar [P] to track soil [P]. Reduced evolutionary lability and adaptability in the nutritional traits of fynbos-specialist lineages may explain the floristic distinctness of the fynbos flora and implies a reduced scope for edaphically driven ecological speciation.

Nitrogen regulation of transpiration controls mass-flow acquisition of nutrients.

Transpiration may enhance mass-flow of nutrients to roots, especially in low-nutrient soils or where the root system is not extensively developed. Previous work suggested that nitrogen (N) may regulate mass-flow of nutrients. Experiments were conducted to determine whether N regulates water fluxes, and whether this regulation has a functional role in controlling the mass-flow of nutrients to roots. Phaseolus vulgaris were grown in troughs designed to create an N availability gradient by restricting roots from intercepting a slow-release N source, which was placed at one of six distances behind a 25 µm mesh from which nutrients could move by diffusion or mass-flow (termed 'mass-flow' treatment). Control plants had the N source supplied directly to their root zone so that N was available through interception, mass-flow, and diffusion (termed 'interception' treatment). 'Mass-flow' plants closest to the N source exhibited 2.9-fold higher transpiration (E), 2.6-fold higher stomatal conductance (gs), 1.2-fold higher intercellular [CO2] (Ci), and 3.4-fold lower water use efficiency than 'interception' plants, despite comparable values of photosynthetic rate (A). E, gs, and Ci first increased and then decreased with increasing distance from the N source to values even lower than those of 'interception' plants. 'Mass-flow' plants accumulated phosphorus and potassium, and had maximum concentrations at 10mm from the N source. Overall, N availability regulated transpiration-driven mass-flow of nutrients from substrate zones that were inaccessible to roots. Thus when water is available, mass-flow may partially substitute for root density in providing access to nutrients without incurring the costs of root extension, although the efficacy of mass-flow also depends on soil nutrient retention and hydraulic properties.

Do hydraulic redistribution and nocturnal transpiration facilitate nutrient acquisition in Aspalathus linearis?

The significance of soil water redistribution by roots and nocturnal transpiration for nutrient acquisition were assessed for deep-rooted 3-year-old leguminous Aspalathus linearis shrubs of the Cape Floristic Region (South Africa). We hypothesised that hydraulic redistribution and nocturnal transpiration facilitate nutrient acquisition by releasing moisture in shallow soil to enable acquisition of shallow-soil nutrients during the summer drought periods and by driving water fluxes from deep to shallow soil powering mass-flow nutrient acquisition, respectively. A. linearis was supplied with sub-surface (1-m-deep) irrigation rates of 0, 2 or 4 L day(-1 )plant(-1). Some plants were unfertilized, whilst others were surface- or deep-fertilized (1 m depth) with Na(15)NO3 and CaP/FePO4. We also supplied deuterium oxide ((2)H2O) at 1 m depth at dusk and measured its predawn redistribution to shallow soil and plant stems. Hydraulic redistribution of deep water was substantial across all treatments, accounting for 34-72 % of surface-soil predawn moisture. Fourteen days after fertilization, the surface-fertilized plants exhibited increased hydraulic redistribution and increased (15)N and P acquisition with higher rates of deep-irrigation. Deep-fertilization also increased hydraulic redistribution to surface soils, although these plants additionally accumulated (2)H2O in their stem tissue overnight, probably due to nocturnal transpiration. Plants engaged in nocturnal transpiration also increased (15)N and P acquisition from deep fertilizer sources. Thus, both nocturnal transpiration and hydraulic redistribution increased acquisition of shallow soil N and P, possibly through a combination of increased nutrient availability and mobility.

How succulent leaves of Aizoaceae avoid mesophyll conductance limitations of photosynthesis and survive drought.

In several taxa, increasing leaf succulence has been associated with decreasing mesophyll conductance (g M) and an increasing dependence on Crassulacean acid metabolism (CAM). However, in succulent Aizoaceae, the photosynthetic tissues are adjacent to the leaf surfaces with an internal achlorophyllous hydrenchyma. It was hypothesized that this arrangement increases g M, obviating a strong dependence on CAM, while the hydrenchyma stores water and nutrients, both of which would only be sporadically available in highly episodic environments. These predictions were tested with species from the Aizoaceae with a 5-fold variation in leaf succulence. It was shown that g M values, derived from the response of photosynthesis to intercellular CO2 concentration (A:C i), were independent of succulence, and that foliar photosynthate δ(13)C values were typical of C3, but not CAM photosynthesis. Under water stress, the degree of leaf succulence was positively correlated with an increasing ability to buffer photosynthetic capacity over several hours and to maintain light reaction integrity over several days. This was associated with decreased rates of water loss, rather than tolerance of lower leaf water contents. Additionally, the hydrenchyma contained ~26% of the leaf nitrogen content, possibly providing a nutrient reservoir. Thus the intermittent use of C3 photosynthesis interspersed with periods of no positive carbon assimilation is an alternative strategy to CAM for succulent taxa (Crassulaceae and Aizoaceae) which occur sympatrically in the Cape Floristic Region of South Africa.

Top-down determinants of niche structure and adaptation among African Acacias.

The role of top-down factors like herbivory and fire in structuring species' niches, even in disturbance-dependent biomes like savanna, remains poorly understood. Interactions between herbivory and fire may set up a potential tradeoff axis, along which unique adaptations contribute to structuring communities and determining species distributions. We examine the role of herbivory and fire in structuring distributions of Acacia saplings in Hluhluwe iMfolozi Park in South Africa, and the relationship of species' niche structure to traits that help them survive herbivory or fire. Results suggest that (1) fire and herbivory form a single trade-off axis, (2) Acacia sapling distributions are constrained by fire and herbivory, and (3) Acacia saplings have adaptations that are structured by the tradeoff axis. Herbivory-adapted species tend to have 'cage'-like architecture, thicker bark, and less starch storage, while fire-adapted species tend to have 'pole'-like architecture, relatively thinner bark, and more starch storage.

Unravelling the limits to tree height: a major role for water and nutrient trade-offs.

Competition for light has driven forest trees to grow exceedingly tall, but the lack of a single universal limit to tree height indicates multiple interacting environmental limitations. Because soil nutrient availability is determined by both nutrient concentrations and soil water, water and nutrient availabilities may interact in determining realised nutrient availability and consequently tree height. In SW Australia, which is characterised by nutrient impoverished soils that support some of the world's tallest forests, total [P] and water availability were independently correlated with tree height (r = 0.42 and 0.39, respectively). However, interactions between water availability and each of total [P], pH and [Mg] contributed to a multiple linear regression model of tree height (r = 0.72). A boosted regression tree model showed that maximum tree height was correlated with water availability (24%), followed by soil properties including total P (11%), Mg (10%) and total N (9%), amongst others, and that there was an interaction between water availability and total [P] in determining maximum tree height. These interactions indicated a trade-off between water and P availability in determining maximum tree height in SW Australia. This is enabled by a species assemblage capable of growing tall and surviving (some) disturbances. The mechanism for this trade-off is suggested to be through water enabling mass-flow and diffusive mobility of P, particularly of relatively mobile organic P, although water interactions with microbial activity could also play a role.

Unequal allocation between male versus female reproduction cannot explain extreme vegetative dimorphism in Aulax species (Cape Proteaceae).

Female plants not only flower but also produce resource-rich seeds, fruits, and cones. Thus, it is generally considered that female plants allocate more resources to sexual reproduction than male plants and that this allocation difference can explain vegetative dimorphism, such as greater leaf size in females. We found significant sexual vegetative differences in the dioecious and serotinous species, Aulax umbellata and A. cancellata. Plant height, annual branch length and canopy spread were greater in males whereas leaf size, branch thickness and branch number were greater in females. Sex ratios and basal stem area were, however, equal in the sexes. Equal sex ratios imply equal allocation to sexual reproduction and equal stem areas imply equal resource use and biomass, and thus allocation to vegetative growth. Given equal allocation to reproduction and resource use, we suggest that the vegetative dimorphism is driven by intra-male-competition to be more visually conspicuous to pollinators. This implies that plant architecture is both a vegetative and a reproductive trait.

Global application of an unoccupied aerial vehicle photogrammetry protocol for predicting aboveground biomass in non-forest ecosystems.

Non-forest ecosystems, dominated by shrubs, grasses and herbaceous plants, provide ecosystem services including carbon sequestration and forage for grazing, and are highly sensitive to climatic changes. Yet these ecosystems are poorly represented in remotely sensed biomass products and are undersampled by in situ monitoring. Current global change threats emphasize the need for new tools to capture biomass change in non-forest ecosystems at appropriate scales. Here we developed and deployed a new protocol for photogrammetric height using unoccupied aerial vehicle (UAV) images to test its capability for delivering standardized measurements of biomass across a globally distributed field experiment. We assessed whether canopy height inferred from UAV photogrammetry allows the prediction of aboveground biomass (AGB) across low-stature plant species by conducting 38 photogrammetric surveys over 741 harvested plots to sample 50 species. We found mean canopy height was strongly predictive of AGB across species, with a median adjusted R 2 of 0.87 (ranging from 0.46 to 0.99) and median prediction error from leave-one-out cross-validation of 3.9%. Biomass per-unit-of-height was similar within but different among, plant functional types. We found that photogrammetric reconstructions of canopy height were sensitive to wind speed but not sun elevation during surveys. We demonstrated that our photogrammetric approach produced generalizable measurements across growth forms and environmental settings and yielded accuracies as good as those obtained from in situ approaches. We demonstrate that using a standardized approach for UAV photogrammetry can deliver accurate AGB estimates across a wide range of dynamic and heterogeneous ecosystems. Many academic and land management institutions have the technical capacity to deploy these approaches over extents of 1-10 ha-1. Photogrammetric approaches could provide much-needed information required to calibrate and validate the vegetation models and satellite-derived biomass products that are essential to understand vulnerable and understudied non-forested ecosystems around the globe.

Mapping soil organic carbon stocks and trends with satellite-driven high resolution maps over South Africa.

Estimation and monitoring of soil organic carbon (SOC) stocks is important for maintaining soil productivity and meeting climate change mitigation targets. Current global SOC maps do not provide enough detail for landscape-scale decision making, and do not allow for tracking carbon sequestration or loss over time. Using an optical satellite-driven machine learning workflow, we mapped SOC stocks (topsoil; 0 to 30 cm) under natural vegetation (86% of land area) over South Africa at 30 m spatial resolution between 1984 and 2019. We estimate a total topsoil SOC stock of 5.6 Pg C with a median SOC density of 6 kg C m-2 (IQR: interquartile range 2.9 kg C m-2). Over 35 years, predicted SOC underwent a net increase of 0.3% (relative to long-term mean) with the greatest net increases (1.7%) and decreases (-0.6%) occurring in the Grassland and Nama Karoo biomes, respectively. At the landscape scale, SOC changes of up to 25% were evident in some locations, as evidenced from fence-line contrasts, and were likely due to local management effects (e.g. woody encroachment associated with increased SOC and overgrazing associated with decreased SOC). Our SOC mapping approach exhibited lower uncertainty (R2 = 0.64; RMSE = 2.5 kg C m-2) and less bias compared to previous low-resolution (250-1000 m) national SOC mapping efforts (average R2 = 0.24; RMSE = 3.7 kg C m-2). Our trend map remains an estimate, pending repeated measures of soil samples in the same location (time-series); a global priority for tracking SOC changes. While high resolution SOC maps can inform land management decisions aimed at climate mitigation (natural climate solutions), potential increases in SOC are likely limited by local climate and soils. It is also important that climate mitigation efforts such as planting trees balance trade-offs between carbon, biodiversity and overall ecosystem function.

Faunal input at host plants: Can camel thorn trees use nutrients imported by resident sociable weavers?

"Islands of fertility" result from the focussing of water and nutrients around many shrub or tree species due to plants foraging for resources. Plant-animal feedbacks may amplify the development of such islands through environmental modification due to, for example, faunal deposition of nutrients and seeds. Fauna residing within vegetation clumps are likely to exert stronger feedbacks on their hosts than itinerant species. We studied the interaction between camel thorn trees (Vachellia erioloba) and the colonial nests of sociable weavers (Philetairus socius) in the Kalahari. We hypothesized that the accumulation of biological material below the nests will alter the nutrient status of the soil beneath the nest trees, in relation to unoccupied trees and the surrounding grassland. We also suggested that this association will have both positive and negative effects on the camel thorn trees. We found that soil concentrations of N, P, and K were, respectively, 4, 4.6, and 1.2 times higher below trees with nests compared to control trees, indicating faunal concentration of nutrients. Soil δ15N values were higher below trees with nests than below control trees without nests. Foliar δ15N values were also higher in nest trees than in control trees, showing the trees accessed faunally derived N. Furthermore, foliar biomass per diameter of terminal branches was 27% higher in nest trees, suggesting that trees respond to nutrient input from the weavers with increased growth. Large barren areas in the subcanopy vegetation directly beneath the colonies were attributed to decreased water infiltration rates, as a result of accumulation of organic matter due to continuous deposition of feces, possibly limiting competitive species from establishing in the subcanopy. On the other hand, canopy volume was reduced in trees with nests due to nests occupying large volumes within the canopy, and nests frequently causing branch fall, indicating costs associated with hosting weaver colonies. Synthesis: We found nutritional benefits to camel thorn trees when hosting sociable weaver colonies. These benefits can potentially overcome important environmental constraints, but these are partially offset by the resulting costs to the host trees.

Fairy circles in Namibia are assembled from genetically distinct grasses.

Fairy circles are striking regularly sized and spaced, bare circles surrounded by Stipagrostis grasses that occur over thousands of square kilometres in Namibia. The mechanisms explaining their origin, shape, persistence and regularity remain controversial. One hypothesis for the formation of vegetation rings is based on the centrifugal expansion of a single individual grass plant, via clonal growth and die-back in the centre. Clonality could explain FC origin, shape and long-term persistence as well as their regularity, if one clone competes with adjacent clones. Here, we show that for virtually all tested fairy circles the periphery is not exclusively made up of genetically identical grasses, but these peripheral grasses belong to more than one unrelated genet. These results do not support a clonal explanation for fairy circles. Lack of clonality implies that a biological reason for their origin, shape and regularity must emerge from competition between near neighbor individuals within each fairy circle. Such lack of clonality also suggests a mismatch between longevity of fairy circles versus their constituent plants. Furthermore, our findings of lack of clonality have implications for some models of spatial patterning of fairy circles that are based on self-organization.

Juggling carbon: allocation patterns of a dominant tree in a fire-prone savanna.

In frequently burnt mesic savannas, trees can get trapped into a cycle of surviving fire-induced stem death (i.e. topkill) by resprouting, only to be topkilled again a year or two later. The ability of savanna saplings to resprout repeatedly after fire is a key component of recent models of tree-grass coexistence in savannas. This study investigated the carbon allocation and biomass partitioning patterns that enable a dominant savanna tree, Acacia karroo, to survive frequent and repeated topkill. Root starch depletion and replenishment, foliage recovery and photosynthesis of burnt and unburnt plants were compared over the first year after a burn. The concentration of starch in the roots of the burnt plants (0.08 +/- 0.01 g g(-1)) was half that of the unburnt plant (0.16 +/- 0.01 g g(-1)) at the end of the first growing season after topkill. However, root starch reserves of the burnt plants were replenished over the dry season and matched that of unburnt plants within 1 year after topkill. The leaf area of resprouting plants recovered to match that of unburnt plants within 4-5 months after topkill. Shoot growth of resprouting plants was restricted to the first few months of the wet season, whereas photosynthetic rates remained high into the dry season, allowing replenishment of root starch reserves. (14)C labeling showed that reserves were initially utilized for shoot growth after topkill. The rapid foliage recovery and the replenishment of reserves within a single year after topkill implies that A. karroo is well adapted to survive recurrent topkill and is poised to take advantage of unusually long fire-free intervals to grow into adults. This paper provides some of the first empirical evidence to explain how savanna trees in frequently burnt savannas are able to withstand frequent burning as juveniles and survive to become adults.

The importance of nutritional regulation of plant water flux.

Transpiration is generally considered a wasteful but unavoidable consequence of photosynthesis, occurring because water is lost when stomata open for CO(2) uptake. Additionally, transpiration has been ascribed the functions of cooling leaves, driving root to shoot xylem transport and mass flow of nutrients through the soil to the rhizosphere. As a consequence of the link between nutrient mass flow and transpiration, nutrient availability, particularly that of NO(3)(-), partially regulates plant water flux. Nutrient regulation of transpiration may function through the concerted regulation of: (1) root hydraulic conductance through control of aquaporins by NO(3)(-), (2) shoot stomatal conductance (g(s)) through NO production, and (3) pH and phytohormone regulation of g(s). These mechanisms result in biphasic responses of water flux to NO(3)(-) availability. The consequent trade-off between water and nutrient flux has important implications for understanding plant distributions, for production of water use-efficient crops and for understanding the consequences of global-change-linked CO(2) suppression of transpiration for plant nutrient acquisition.

Nutrient availability moderates transpiration in Ehrharta calycina.

Transpiration-driven 'mass-flow' of soil-water can increase nutrient flow to the root surface. Here it was investigated whether transpiration could be partially regulated by nutrient status. Seeds of Ehrharta calycina from nine sites across a rainfall gradient were supplied with slow-release fertilizer dibbled into the sand surrounding the roots and directly available through interception, mass-flow and diffusion (dubbed 'interception'), or sequestered behind a 40-microm mesh and not directly accessible by the roots, but from which nutrients could move by diffusion or mass-flow (dubbed 'mass-flow'). Although mass-flow plants were significantly smaller than interception plants as a consequence of nutrient limitation, they transpired 60% faster, had 90% higher photosynthesis relative to transpiration (A/E), and 40% higher tissue P, Ca and Na concentrations than plants allowed to intercept nutrients directly. Tissue N and K concentrations were similar for interception and mass-flow plants. Transpiration was thus higher in the nutrient-constrained 'mass-flow' plants, increasing the transport of nutrients to the roots by mass-flow. Transpiration may have been regulated by N availability, resulting in similar tissue concentration between treatments. It is concluded that, although transpiration is a necessary consequence of photosynthetic CO(2) uptake in C(3) plants, plants can respond to nutrient limitation by varying transpiration-driven mass-flow of nutrients.

Root of edaphically controlled Proteaceae turnover on the Agulhas Plain, South Africa: phosphate uptake regulation and growth.

The influence of phosphorus (P) availability on growth and P uptake was investigated in South African Proteaceae: (1) Protea compacta R.Br., endemic on severely nutrient-impoverished colluvial sands; (2) Protea obtusifolia Bueck ex Meissner; and (3) Leucadendron meridianum I. J. Williams, the latter both endemic on comparatively fertile limestone-derived soils. Plants were grown hydroponically in 1000 L tanks at 0.01, 0.1 or 1.0 microm P for 14 weeks. Biomass accumulation was influenced by P availability, doubling as [P] increased from 0.1 to 1.0 microm. Total biomass was greatest for P. compacta, but L. meridianum and P. obtusifolia had two to four times greater relative biomass accumulation at 0.1 and 1.0 microm [P]. Proteoid root clusters developed at both 0.01 and 0.1 microm[P], but were suppressed at 1.0 microm [P]; this was a 10-fold lower [P] than previously reported to inhibit cluster root formation. Rates of net P uptake at 5 microm P decreased in response to increased P availability from 0.01 to 1.0 microm P. Significant between-species differences in rates of P uptake and capacity to down-regulate P uptake were observed: P. compacta < P. obtusifolia < L. meridianum. The species responses are discussed in terms of adaptation to mosaics in soil P availability and the high beta diversity in the natural habitat.

Quantifying N-loss by root abscission: consequences for wheat N budgets and δ15N values.

Lower plant δ15N values relative to source δ15N are commonly attributed to 15N efflux. We determined the extent to which root abscission contributes to plant N-loss and consequences for plant δ15N. Wheat (Triticum aestivum L. cv. SST015) was grown in hydroponics with direct aeration, aeration constrained within a pipe and circulation of nutrient solution through sand, representing three levels of stability for root growth. The δ15N of nutrient solutions and root fragments were periodically determined, as well as root and shoot δ15N. Plants in solution had significantly more negative δ15N (-8.9 and -9.2‰) than plants in sand (-6.9‰), suggesting greater 15N-loss; root fragments were major biomass- (six-fold greater than root dry weight) and N-loss (two-fold greater than plant net N uptake) pathways in solution. These plants had more ephemeral roots and two-fold more root tips than the sand treatment. We estimated that root fragment loss decreased plant δ15N by at least -3.7, -2.6 and -1.0‰ in the direct, pipe and sand treatments, respectively. Positive nutrient solution δ15N in all treatments relative to the source δ15N suggests that plant N, probably derived from efflux, was present in solution. Despite this, root abscission and root turnover are also important N-loss pathways in plants, while plant δ15N values are probably influenced by a combination of root abscission and N efflux.