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1.
Vet Pathol ; : 3009858241286828, 2024 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-39400051

RESUMO

Feline chronic enteropathy is a poorly defined condition of older cats that encompasses chronic enteritis to low-grade intestinal lymphoma. The histological evaluation of lymphocyte numbers and distribution in small intestinal biopsies is crucial for classification and grading. However, conventional histological methods for lymphocyte quantification have low interobserver agreement, resulting in low diagnostic reliability. This study aimed to develop and validate an artificial intelligence (AI) model to detect intraepithelial and lamina propria lymphocytes in hematoxylin and eosin-stained small intestinal biopsies from cats. The median sensitivity, positive predictive value, and F1 score of the AI model compared with the majority opinion of 11 veterinary anatomic pathologists, were 100% (interquartile range [IQR] 67%-100%), 57% (IQR 38%-83%), and 67% (IQR 43%-80%) for intraepithelial lymphocytes, and 89% (IQR 71%-100%), 67% (IQR 50%-82%), and 70% (IQR 43%-80%) for lamina propria lymphocytes, respectively. Errors included false negatives in whole-slide images with faded stain and false positives in misidentifying enterocyte nuclei. Semiquantitative grading at the whole-slide level showed low interobserver agreement among pathologists, underscoring the need for a reproducible quantitative approach. While semiquantitative grade and AI-derived lymphocyte counts correlated positively, the AI-derived lymphocyte counts overlapped between different grades. Our AI model, when supervised by a pathologist, offers a reproducible, objective, and quantitative assessment of feline intestinal lymphocytes at the whole-slide level, and has the potential to enhance diagnostic accuracy and consistency for feline chronic enteropathy.

2.
Vet Pathol ; 59(1): 169-172, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34493121

RESUMO

The cestode Hymenolepis nana is a common parasite of humans and mice. Fecal shedding in the absence of clinical disease has previously been reported in ring-tailed lemurs (Lemur catta). This report describes fatal, disseminated H. nana cestodiasis infection in an aged ring-tailed lemur in a zoological collection. The parasites were associated with severe multifocal to coalescing and regionally extensive pyogranulomatous hepatitis and moderate multifocal pneumonia. The morphology of the parasites was highly unusual. Profiles were variably sized, ellipsoid to irregularly serpiginous, lined by a thin tegument, and filled with lightly eosinophilic fibrillar stroma and numerous, round basophilic cells. Polymerase chain reaction targeting a portion of the 18S rRNA gene and DNA sequencing of the amplicon showed 100% homology with H. nana.


Assuntos
Hymenolepis nana , Lemur , Animais , Fezes , Camundongos
3.
J Zoo Wildl Med ; 51(4): 856-867, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33480566

RESUMO

Dental and oral diseases are prevalent in many mammalian species including wild felids. Determining the dental and oral health status of captive animal populations can help establish preventive and therapeutic strategies, leading to improved welfare and conservation efforts. The aim of this study was to assess the prevalence of periodontal disease, endodontic disease, tooth resorption, and other clinically relevant dental and maxillofacial abnormalities in a population of captive jaguars (Panthera onca) using clinical, radiographic, and histopathological findings. Fifteen jaguars, ranging from young adult to geriatric, kept at a private zoo in Belize, Central America, had a detailed oral examination under general anesthesia between January 2015 and March 2019. Periodontitis was present in 3.8% (16/423) of examined teeth and 53.8% (7/13) of jaguars that underwent periodontal probing. Endodontic disease secondary to dentoalveolar trauma was found in 21.0% (89/423) of teeth in 73.3% (11/15) of animals. Tooth resorption, which has not been previously documented in jaguars, affected 1.4% (6/423) of teeth in 13.3% (2/15) of jaguars. Other abnormalities included metallic foreign material (gunshot) identified radiographically in 33.3% (5/15) of jaguars and nontraumatizing malocclusion in 9.1% (1/11) of jaguars that had occlusion evaluated. Much of the oral pathology identified in captive jaguars is suspected to arise from capture and/or captivity-associated behaviors, as suggested by gunshot around the oral cavity, fractures of rostral teeth (canine and incisor teeth), and abrasions consistent with cage-biting on canine teeth. Anesthetized oral examination-including full-mouth intraoral radiographs, periodontal probing, and charting-is recommended for jaguars with clinical signs of oral pain, as well as for routine systemic evaluation.


Assuntos
Doenças da Boca/veterinária , Panthera , Doenças Dentárias/veterinária , Animais , Animais de Zoológico , Belize , Feminino , Masculino , Doenças da Boca/patologia , Doenças Dentárias/patologia , Doenças Dentárias/cirurgia
4.
Can Vet J ; 61(6): 584-588, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32675809

RESUMO

A 3-year-old intact female Labradoodle bitch was referred due to fever and lethargy 4 days postpartum. The dog was reported to have had prolonged labor that required assistance and fetal membranes were retained. Physical examination and diagnostics led to a suspicion of metritis and uterine perforation. Ovariohysterectomy was performed. Gross and histopathology findings revealed multifocal uterine perforation, necrosuppurative metritis, and placenta percreta. Post-operative antibiotic therapy and supportive care resulted in an uneventful clinical recovery. This is the first reported case of placenta percreta in a bitch. It is presumed that this pathology was paramount in the patient's development of metritis and subsequent uterine rupture. Key clinical message: Placenta percreta may lead to more severe clinical consequences of metritis, including uterine rupture.


Perforation utérine secondaire à une métrite et un placenta percreta chez une chienne en période post-partum. Une femelle Labradoodle intacte âgée de 3 ans fut référée pour cause de fièvre et léthargie 4 jours post-partum. Il fut rapporté que la chienne avait eu un travail long qui demanda de l'assistance et qu'il y avait eu rétention des membranes foetales. L'examen physique et le diagnostic mena à un doute de métrite et de perforation utérine. Une ovario-hystérectomie fut réalisée. Les trouvailles de pathologie macroscopique et d'histopathologie révélèrent des perforations utérines multifocales, une métrite nécro-suppurative et un placenta percreta. Une antibiothérapie post-opératoire et des soins de support ont résulté en une guérison clinique sans conséquence. Ceci représente le premier cas rapporté de placenta percreta chez une chienne. Il est présumé que chez cette chienne cette pathologie était vitale dans le développement de la métrite et de la rupture utérine subséquente.Message clinique clé :Un placenta percreta peut mener à des conséquences cliniques plus sévères de métrite, incluant la rupture utérine.(Traduit par Dr Serge Messier).


Assuntos
Doenças do Cão , Placenta Acreta , Perfuração Uterina , Ruptura Uterina , Animais , Doenças do Cão/tratamento farmacológico , Doenças do Cão/etiologia , Cães , Feminino , Histerectomia/veterinária , Placenta Acreta/cirurgia , Placenta Acreta/veterinária , Período Pós-Parto , Gravidez , Perfuração Uterina/veterinária , Ruptura Uterina/veterinária
5.
Can Vet J ; 60(5): 495-501, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-31080262

RESUMO

A 6-year-old Standardbred mare was presented at 339 days of gestation for investigation of abnormal abdominal distension and ventral edema. Transrectal palpation and ultrasound examination revealed the uterus to be enlarged with an excessive volume of fetal fluid, characteristic of hydrops. Gradual transcervical drainage of 55 L of allantoic fluid over 45 minutes, with concurrent intravenous fluid therapy followed by assisted vaginal delivery, resulted in the birth of a live foal with long-term survival. The birth and long-term survival of a foal from a mare with hydrallantois at term has not been previously reported in horses. However, this report demonstrates that successful outcome for both mare and foal may be achieved in a mare at term with hydrallantois.


Gestion réussie de l'hydrallantois chez une jument Standardbred à terme donnant lieu à la naissance d'un poulain vivant. Une jument Standardbred âgée de 6 ans a été présentée à 339 jours de gestation pour investiguer une distension abdominale anormale et un oedème ventral. La palpation transrectale et l'échographie ont révélé que l'utérus était enflé en raison d'un volume excessif de liquide foetal, ce qui est caractéristique de l'hydrops fetalis. Un drainage transcervical graduel de 55 L de liquide allantoïdien pendant plus de 45 minutes et une fluidothérapie par intraveineuse suivis d'une mise bas vaginale assistée ont donné lieu à la naissance d'un poulain vivant avec survie à long terme. La naissance et la survie à long terme d'un poulain provenant d'une jument atteinte de l'hydrallantois à terme n'avaient pas été précédemment signalées chez les chevaux. Cependant, des résultats fructueux pour la jument et le poulain peuvent être obtenus chez une jument atteinte d'hydrallantois à terme.(Traduit par Isabelle Vallières).


Assuntos
Gastroenteropatias/veterinária , Doenças dos Cavalos , Parto , Animais , Edema/veterinária , Feminino , Cavalos , Gravidez
6.
J Wildl Dis ; 60(3): 783-785, 2024 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-38533628

RESUMO

Lower respiratory tract disease associated with mycoplasmal infection was detected in a free-ranging bog turtle (Glyptemys muhlenbergii) from New Jersey, USA. The presence of a mycoplasmal organism was confirmed by PCR and electron microscopy. Fluid-filled lungs were observed grossly, and there was proliferative pneumonia on histopathology.


Assuntos
Tartarugas , Animais , Tartarugas/microbiologia , Infecções por Mycoplasma/veterinária , Infecções por Mycoplasma/microbiologia , Infecções por Mycoplasma/patologia , Feminino , Masculino
7.
Viruses ; 16(9)2024 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-39339954

RESUMO

Viruses in the subfamily Serpentovirinae (order Nidovirales, family Tobaniviridae) can cause significant morbidity and mortality in captive snakes, but documented infections have been limited to snakes of the Boidae, Colubridae, Homalopsidae, and Pythonidae families. Infections can either be subclinical or associated with oral and/or respiratory disease. Beginning in June 2019, a population of over 150 confiscated snakes was screened for serpentovirus as part of a quarantine disease investigation. Antemortem oropharyngeal swabs or lung tissue collected postmortem were screened for serpentovirus by PCR, and 92/165 (56.0%) of snakes tested were positive for serpentovirus. Serpentoviruses were detected in fourteen species of Viperidae native to Asia, Africa, and South America and a single species of Elapidae native to Australia. When present, clinical signs included thin body condition, abnormal behavior or breathing, stomatitis, and/or mortality. Postmortem findings included variably severe inflammation, necrosis, and/or epithelial proliferation throughout the respiratory and upper gastrointestinal tracts. Genetic characterization of the detected serpentoviruses identified four unique viral clades phylogenetically distinct from recognized serpentovirus genera. Pairwise uncorrected distance analysis supported the phylogenetic analysis and indicated that the viper serpentoviruses likely represent the first members of a novel genus in the subfamily Serpentovirinae. The reported findings represent the first documentation of serpentoviruses in venomous snakes (Viperidae and Elapidae), greatly expanding the susceptible host range for these viruses and highlighting the importance of serpentovirus screening in all captive snake populations.


Assuntos
Filogenia , Serpentes , Animais , Serpentes/virologia , Viperidae/virologia , Nidovirales/genética , Nidovirales/classificação , Nidovirales/isolamento & purificação , Infecções por Nidovirales/veterinária , Infecções por Nidovirales/virologia , Genoma Viral
8.
J Wildl Dis ; 58(3): 512-523, 2022 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-35704503

RESUMO

Research on host response to infectious disease often involves pharmacological induction of immunosuppression, frequently through administration of dexamethasone. Reports on the effect of dexamethasone in birds are largely restricted to poultry and pigeons. This study describes changes in white blood cell (WBC) differentials, hemoparasite counts, splenic histology, and splenic CD3 immunoreactivity in House Finches (Haemorhous mexicanus). Experimental group birds (n=9) were treated with a daily intramuscular injection of 25 µg of dexamethasone for 8 d; a control group (n=9) received daily saline solution. Smears were made with blood collected immediately before the first dose (day 0) and on d 4, 8, and 9, and stained with modified Wright. The WBC differential counts were performed by three blinded observers, parasite counts by two blinded observers, and histology by one blinded observer. Dexamethasone-treated birds experienced relative heterophilia and lymphopenia on d 4 (P=0.008); heterophilia was also present at d 8 (P=0.018). Hemosporidian counts were significantly increased in dexamethasone-treated birds on d 4 and 8 (P=0.048 and P=0.031, respectively). In contrast with control birds, all dexamethasone-treated birds lacked histologically apparent splenic lymphoid follicles (P<0.001). No significant difference was observed in splenic CD3 immunoreactivity between groups. Our results indicate that dexamethasone has an effect on the hematologic profile of House Finches and suggest that it may be a useful method to induce immunosuppression in this species.


Assuntos
Doenças das Aves , Tentilhões , Infecções por Mycoplasma , Animais , Doenças das Aves/tratamento farmacológico , Dexametasona/farmacologia , Tentilhões/fisiologia , Infecções por Mycoplasma/veterinária
9.
J Comp Pathol ; 186: 51-61, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34340804

RESUMO

The bearded dragon (Pogona vitticeps) is a common species in the pet reptile trade and in zoological collections. Despite this, only a few reviews detailing common causes of mortality have been published. The goal of this retrospective study was to compile information related to the presence and prevalence of various diseases in bearded dragons in several private and one zoological collection. Findings from 36 animals, necropsied over a 20-year period were categorized on the basis of the cause of death or euthanasia and the organ system affected. Multiple contributors to death were identified in a subset of animals (n = 12; 33.33%) and were counted in more than one category. The most common contributors to death by category were inflammatory (infectious or non-infectious) diseases (n = 25; 69.44%), followed by chronic/degenerative diseases (n = 17; 47.22%). Diseases or conditions that were systemic, or affected the hepatobiliary system, were the most commonly implicated in death. The most frequent histological findings included hepatic fatty change, renal tubular epithelial pigmentation and the presence of faveolar hyaline-like material. Although Chi square testing did not reveal an association between these common findings and comorbidities, a statistically significant association was found between chronic/degenerative causes of death and renal tubular epithelial pigment. Chi square testing of categorical variables, including contributors to death, primary organ(s) affected, sex, age class and institution, identified statistically significant associations between males and chronic/degenerative disease (P = 0.043) and inflammatory contributors to death and private collection animals (P = 0.039). Death due to inflammatory contributors was significantly higher in the 1-5-year-old and >5-year-old age classes (P = 0.02).


Assuntos
Doenças dos Animais/patologia , Lagartos , Animais , Masculino , Estudos Retrospectivos
10.
J Am Vet Med Assoc ; : 1-7, 2021 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-34890364

RESUMO

CASE DESCRIPTION: Outbreaks of sudden death in apparently healthy weaned dairy calves due to Strongyloides papillosus parasitism were diagnosed on 2 separate and independent New York (NY) dairies. CLINICAL FINDINGS: Most calves were found dead; however, 1 calf observed while dying showed signs of tachycardia, tachypnea, vocalization, and convulsions shortly before death. In 6 affected heifers that underwent post-mortem examination, precocious bilaterally symmetric mammary gland enlargement was seen. A portion of their parasitized living cohorts also demonstrated similar mammary gland enlargement. A diagnosis of S papillosus hyperinfection was made based upon the presence of high numbers of S papillosus ova in feces, and confirmation by S papillosus-specific PCR assays. Consistent histopathological findings in affected calves included generalized mammary gland vascular congestion, interstitial edema and hemorrhage with ductal hyperplasia. Mild multifocal cardiomyocyte degeneration was found in 5 of 14 calves examined. Factors believed to contribute to the parasite's environmental amplification and host hyperinfection included group housing on wood shavings and high environmental temperatures and humidity. TREATMENT AND OUTCOME: Treatment of calves with doramectin pour-on stopped mortality and resolved the udder enlargement. CLINICAL RELEVANCE: Similar outbreaks have previously been described in Japan and South Bohemia (Czech Republic), where researchers hypothesized that sudden death may be due to fatal arrhythmia caused by a parasite-associated cardiotoxin. This report highlights the importance of including S papillosus among the differential diagnoses for sudden death alone or together with precocious udder enlargement in calves kept in confinement housing.

11.
J Vet Diagn Invest ; 32(5): 722-726, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32715936

RESUMO

Twenty-eight lactating dairy cattle in New York State were exposed to botulism toxin; 12 died and 16 recovered but never returned to full productivity. Pieces of a raccoon carcass were found in the total mixed ration on the first day of the outbreak. Clinical signs included anorexia, decreased milk production, decreased tongue tone, profound weakness, and recumbency. Clostridium botulinum type A (BoNT/A) was detected in rumen contents from 2 deceased cows via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). In addition, C. botulinum type C was cultured from the liver of a third cow, and C. botulinum neurotoxin-producing type C gene (bont/C) was detected via real-time PCR. On postmortem examination, 4 cows had findings suggestive of toxic myopathy, but the cause and significance of these lesions is unknown given that botulism is typically not associated with gross or histologic lesions. This outbreak of BoNT/A in cattle in North America was diagnosed via MALDI-TOF MS, a rapid and sensitive modality for detection of botulinum preformed neurotoxin.


Assuntos
Botulismo/veterinária , Doenças dos Bovinos/diagnóstico , Surtos de Doenças/veterinária , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/veterinária , Animais , Toxinas Botulínicas/análise , Botulismo/diagnóstico , Botulismo/epidemiologia , Bovinos , Doenças dos Bovinos/epidemiologia , Clostridium botulinum/isolamento & purificação , Feminino , New York/epidemiologia
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