An analysis of genetic stock identification on a small geographical scale using microsatellite markers, and its application in the management of a mixed-stock fishery for Atlantic salmon Salmo salar in Ireland.

A genetic stock identification (GSI) study was undertaken in a fishery for Atlantic salmon Salmo salar to determine the effects of restrictive fishery management measures on the stock composition of the fishery, and if accurate and precise stock composition estimates could be achieved on the small geographical scale where this fishery operates, using a suite of only seven microsatellite loci. The stock composition of the Foyle fishery was shown to comprise almost exclusively of Foyle origin fish in the 3 years after restrictive measures were introduced in 2007, compared to 85% the year before. This showed that the restrictive measures resulted in the Foyle fishery being transformed from a mixed-stock fishery to an almost exclusively single-stock fishery, and showed how GSI studies can guide and evaluate management decisions to successfully manage these fisheries. Highly accurate and precise stock composition estimates were achieved in this study, using both cBAYES and ONCOR genetic software packages. This suggests accurate and precise stock composition is possible even on small geographical scales.

Beaufort trout MicroPlex: a high-throughput multiplex platform comprising 38 informative microsatellite loci for use in resident and anadromous (sea trout) brown trout Salmo trutta genetic studies.

A flexible panel consisting of 38 informative microsatellite markers for Salmo trutta is described. These markers were selected from a pool of over 150 candidate loci that can be readily amplified in four multiplex PCR groups but other permutations are also possible. The basic properties of each markers were assessed in six population samples from both the Burrishoole catchment, in the west of Ireland, and Lough Neagh, in Northern Ireland. A method to assess the relative utility of individual markers for the detection of population genetic structuring is also described. Given its flexibility, technical reliability and high degree of informativeness, the use of this panel of markers is advocated as a standard for S. trutta genetic studies.

The effect of stocking with 0+ year age-class Atlantic salmon Salmo salar fry: a case study from the River Bush, Northern Ireland.

An enhancement programme based on stocking 0+ year age-class Atlantic salmon Salmo salar, conducted in the River Bush, Northern Ireland, U.K. over the period 1996-2005, was reviewed with reference to the performance and biological characteristics of wild fish. Wild ova to 0+ year fry (summer) survival was c. 8% with subsequent wild 0+ year fry-to-smolt survival c. 9%. Stocked unfed 0+ year juveniles gave c. 1% survival to smolt whilst fed 0+ year S. salar stocked in late summer exhibited survival at c. 5%. Stocking with unfed and fed fry contributed to increased smolt production and helped attain local management objectives between 2001 and 2005. Significant differences in biological characteristics were observed between wild and stocked-origin fish. Wild-smolt cohorts were dominated by 2+ year age-class fish on the River Bush whilst smolts originating from fed fry mostly comprised younger 1+ year individuals. The mean mass of 1+ year smolts derived from stocked fed fry was significantly lower than that of wild 1+ year smolts, although these differences were not evident between older age classes. Differences in run timing between wild smolts and smolts derived from stocked fry were also apparent with the stocked-origin fish tending to run earlier than wild fish. Although the stocking exercise was useful in terms of maximizing freshwater production, concerns over the quality of stocked-origin recruits and the long term consequences for productivity are highlighted.

Microsatellite standardization and evaluation of genotyping error in a large multi-partner research programme for conservation of Atlantic salmon (Salmo salar L.).

Microsatellite genotyping is a common DNA characterization technique in population, ecological and evolutionary genetics research. Since different alleles are sized relative to internal size-standards, different laboratories must calibrate and standardize allelic designations when exchanging data. This interchange of microsatellite data can often prove problematic. Here, 16 microsatellite loci were calibrated and standardized for the Atlantic salmon, Salmo salar, across 12 laboratories. Although inconsistencies were observed, particularly due to differences between migration of DNA fragments and actual allelic size ('size shifts'), inter-laboratory calibration was successful. Standardization also allowed an assessment of the degree and partitioning of genotyping error. Notably, the global allelic error rate was reduced from 0.05 ± 0.01 prior to calibration to 0.01 ± 0.002 post-calibration. Most errors were found to occur during analysis (i.e. when size-calling alleles; the mean proportion of all errors that were analytical errors across loci was 0.58 after calibration). No evidence was found of an association between the degree of error and allelic size range of a locus, number of alleles, nor repeat type, nor was there evidence that genotyping errors were more prevalent when a laboratory analyzed samples outside of the usual geographic area they encounter. The microsatellite calibration between laboratories presented here will be especially important for genetic assignment of marine-caught Atlantic salmon, enabling analysis of marine mortality, a major factor in the observed declines of this highly valued species.

Evidence of changing migratory patterns of wild Atlantic salmon Salmo salar smolts in the River Bush, Northern Ireland, and possible associations with climate change.

The migration patterns, timing and biological characteristics of wild Atlantic salmon Salmo salar smolts in the River Bush, Northern Ireland, were examined over the period 1978-2008. A distinct change in the timing of the smolt run was detected with progressively earlier emigration periods evident across the time series. The shift in run timing ranged from 3.6 to 4.8 days 10 years(-1) for a range of standard migratory audit points. The timing of smolt emigration has been linked to ambient river temperature patterns. Distinct seasonal patterns were evident for biological characteristics of River Bush smolts with mean age and fork length decreasing throughout the emigration period. Marine survival patterns in 1 sea winter River Bush S. salar were strongly influenced by the run timing of the preceding smolt year such that later emigrating cohorts demonstrated increased survival. Possible mechanisms for this relationship based on local climatic variation have been explored, including the effect of potential thermal mismatch between freshwater and marine environments.

Italian validation of the Children's Shyness Questionnaire: Exploring associations between shyness and psychosocial functioning.

BACKGROUND: In recent years, researchers have begun to explore the implications of shyness for the psychosocial wellbeing of children and adolescents, exploring its association with internalizing problems. Research in an Italian context is hindered by the lack of a validated self-report measure of shyness. We report two studies aimed to assess the psychometric properties of an Italian translation of the Children's Shyness Questionnaire (CSQ-it) and investigate its correlations with convergent and divergent constructs. The first study aimed to examine associations between CSQ-it and self-report measures of anxiety and somatic symptoms and attachment with parents and peers. The second study aimed to investigate its relations to internet addiction. METHODS: The self-report measures were completed by 550 participants in the first study and 131 participants in the second study. Parents provided information on their child's problems. Psychometric properties were assessed by Cronbach's alpha in both studies and by exploratory and confirmatory factor analysis in Study 1. The relations between shyness and measures of internalizing problems and attachments were analyzed by correlational methods. In Study 2 a moderated mediation model tested the hypothesis that the relationship between shyness and internet addiction is mediated by somatic symptoms and that shyness moderates the relationship between somatic symptoms and internet addiction. RESULTS: The reliability and validity of the Italian Version of the Children's Shyness Questionnaire were satisfactory. Results from confirmatory factor analyses confirmed the single-factor model of the questionnaire previously identified in North American and Chinese studies. There were significant correlations between shyness, anxious and somatic symptomatology, impaired psychosocial functioning and specific components of attachment relationships. In Study 2 the indirect effect of shyness on internet addiction through somatic symptoms was significant as well as significantly moderated for high shyness scores but not for low levels of shyness. CONCLUSION: To our knowledge this is the first study that explored the psychometric proprieties of the Children's Shyness Questionnaire in the Italian context. Findings demonstrated that this self-reported measure of shyness has sound psychometric properties and can be used as a sensitive and appropriate instrument for the assessment of shyness in children and adolescents.

Dispositional and situational learning goals and children's self-regulation.

BACKGROUND: Little research has examined interactions between self-reported dispositional and experimentally manipulated situational group orientations in their effect on self-regulation. AIMS: The aim of the study was to investigate the effect of dispositional and situational learning goal orientation on children's self-efficacy and engagement and persistence at a puzzle task. SAMPLE: A self-report learning goal orientation scale was completed by 110 children, aged 9-11 years. Fifty-three children (24 girls) selected to be high and low on the scale participated in the experiment. METHODS: Half of the children were given instructions designed to evoke learning goals, while the remainder received performance goal instructions. Children attempted a difficult puzzle task on two occasions, when measures were made of self-regulatory behaviours. RESULTS AND CONCLUSIONS: Children assigned to the learning goal instruction were more likely to persist at the task until the end of the allotted time, displayed more on-task behaviour and engaged in more autonomous help-seeking. These effects were more pronounced following the first task, which all children had been unable to complete. Dispositional task orientation did not predict individual differences on these measures. The findings are interpreted in terms of learned helplessness and self-worth theory.

Multicompartmental analysis of [11C]-carfentanil binding to opiate receptors in humans measured by positron emission tomography.

[11C]-Carfentanil is a high affinity opiate agonist that can be used to localize mu opiate receptors in humans by positron emission tomography (PET). A four-compartment model was used to obtain quantitative estimates of rate constants for receptor association and dissociation. PET studies were performed in five normal subjects in the absence and presence of 1 mg/kg naloxone. Arterial plasma concentration of [11C]-carfentanil and its labeled metabolites were determined during each PET study. The value of k3/k4 = Bmax/kD was determined for each subject in the presence and absence of naloxone. There was a significant reduction in the value of k3/k4 from 3.4 +/- 0.92 to 0.26 +/- 0.13 in the thalamus (p less than 0.01) and from 1.8 +/- 0.33 to 0.16 +/- 0.065 in the frontal cortex (p less than 0.001). Mean values of frontal cortex/occipital cortex and thalamus/occipital cortex ratios were determined for the interval 35-70 min after injection when receptor binding is high relative to nonspecific binding. The relationship between the measured region/occipital cortex values and the corresponding values of k3/k4 in the presence and absence of naloxone was: regions/occipital cortex = 0.95 + 0.74 (k3/k4) with r = 0.98 (n = 20). Simulation studies also demonstrated a linear relationship between the thalamus/occipital cortex or frontal cortex/occipital cortex ratio and k3/k4 for less than twofold increases or decreases in k3/k4. Simulation studies in which thalamic blood flow was varied demonstrated no significant effect on the region/occipital cortex ratio at 35-70 min for a twofold increase or fourfold decrease in blood flow. Therefore, the region/occipital cortex ratio can be used to quantitate changes in k3/k4 when tracer kinetic modeling is not feasible.

Phobic anxiety in 11 nations. Part I: Dimensional constancy of the five-factor model.

The Fear Survey Schedule-III (FSS-III) was administered to a total of 5491 students in Australia, East Germany, Great Britain, Greece, Guatemala, Hungary, Italy, Japan, Spain, Sweden, and Venezuela, and submitted to the multiple group method of confirmatory analysis (MGM) in order to determine the cross-national dimensional constancy of the five-factor model of self-assessed fears originally established in Dutch, British, and Canadian samples. The model comprises fears of bodily injury-illness-death, agoraphobic fears, social fears, fears of sexual and aggressive scenes, and harmless animals fears. Close correspondence between the factors was demonstrated across national samples. In each country, the corresponding scales were internally consistent, were intercorrelated at magnitudes comparable to those yielded in the original samples, and yielded (in 93% of the total number of 55 comparisons) sex differences in line with the usual finding (higher scores for females). In each country, the relatively largest sex differences were obtained on harmless animals fears. The organization of self-assessed fears is sufficiently similar across nations to warrant the use of the same weight matrix (scoring key) for the FSS-III in the different countries and to make cross-national comparisons feasible. This opens the way to further studies that attempt to predict (on an a priori basis) cross-national variations in fear levels with dimensions of national cultures.

Shyness and self-esteem in middle childhood.

The relationship between shyness and self-esteem was assessed for two samples of children aged 9 to 12 years. Shyness was measured by a new self-report questionnaire based on an elicitation of children's conceptions of shyness. Shyness was significantly correlated with measures of global self-esteem, with external locus of control and with perceived competence across different domains of the self. The relationship among variables were similar to those reported for adolescents and adults. Girls were more shy than boys, and there was a suggestion that 11-year-old children were more shy in secondary than in primary school.

The influence of shyness on children's test performance.

BACKGROUND: Research has shown that shy children differ from their peers not only in their use of language in routine social encounters but also in formal assessments of their language development, including psychometric tests of vocabulary. There has been little examination of factors contributing to these individual differences. AIMS: To investigate cognitive-competence and social anxiety interpretations of differences in children's performance on tests of vocabulary. To examine the performance of shy and less shy children under different conditions of test administration, individually with an examiner or among their peers within the familiar classroom setting. SAMPLE: The sample consisted of 240 Year 5 pupils (122 male, 118 female) from 24 primary schools. METHOD: Shy and less shy children, identified by teacher nomination and checklist ratings, completed vocabulary and mental arithmetic tests in one of three conditions, in a between-subjects design. The conditions varied individual and group administration, and oral and written responses. RESULTS: The conditions of test administration influenced the vocabulary test performance of shy children. They performed significantly more poorly than their peers in the two face-to-face conditions but not in the group test condition. A comparable trend for the arithmetic test was not statistically significant. Across the sample as a whole, shyness correlated significantly with test scores. CONCLUSIONS: Shyness does influence children's cognitive test performance and its impact is larger when children are tested face-to-face rather than in a more anonymous group setting. The results are of significance for theories of shyness and have implications for the assessment of schoolchildren.

Blushing, embarrassability and self-consciousness.

A blushing questionnaire and the Self-Consciousness scale (SCS) were completed by 86 respondents. A measure of the tendency to blush correlated significantly with the Social Anxiety subscale of the SCS, but not with the public and private subscales. Self-rated tendency to blush was significantly correlated with rated likelihood of blushing in those situations where the individual had some responsibility for an embarrassing incident, but not in situations where others had instigated the incident.

Name-calling and nicknames in a sample of primary school children.

BACKGROUND: Name-calling, unkind nicknames and other forms of verbal harassment represent some of the most prevalent forms of bullying in school but they have been little studied. Name-calling and nicknames in particular are ambiguous social events that can serve positive as well as negative goals, and their adverse consequences can be difficult to identify. AIMS: (i) To assess the incidence of nicknames and name calling as reported by a sample of primary school children; (ii) to examine the kinds of names reported by children, and to relate these to names reported in other social contexts; (iii) to explore the impact that name-calling and nicknames have on children. SAMPLE: Pupils (N = 60) from the top two classes in a British primary school completed a questionnaire; 20 of the children were subsequently interviewed. METHOD: Pupils completed a questionnaire that was constructed for this study. Pupils were asked to provide examples of nicknames and to report on the types and incidence of several forms of verbal harassment. The interview included questions which aimed to explore the children's reactions to harassment. RESULTS: Being called disliked nicknames, called names, teased, and other forms of verbal harassment were reported by most of the sample, with more than 20% of children experiencing nasty comments and unkind nicknames on a daily basis. Girls reported more disliked nicknames than boys. The most common nicknames referred to the child's appearance, whereas nasty comments and untrue stories contained a preponderance of sexual references. In the interview, nearly all children reported that being called names and nicknames were negative experiences that caused distress. CONCLUSIONS: Name-calling and the assignment of unkind nicknames are prevalent and hurtful++ features of school life. The kinds of names are similar to those reported in other studies of children, adolescents, and adults. It is proposed that these names are hurtful because they threaten the child's identity.

In praise of blushing.

Many people are extremely anxious about their blushing and regard it as the cause of their social difficulties rather than as a symptom of them. This paper addresses the belief that there is something inherently unpleasant or unattractive about blushing. Historical evidence shows that redness of the cheeks has long been regarded as a sign of facial beauty and youth, and transitory changes such as the blush have also been seen in a positive light as an indication of modesty and charm. The blush is an expression of shame and embarrassment and it too can be regarded in positive or negative light depending on the circumstances. What is distinctive about contemporary anxieties about blushing is that perceived personal inadequacies are in themselves reason to be ashamed and embarrassed: there is a vicious circle where a blush is both a sign of, and reason for, self-deprecation. This perception is not a necessary one but reflects current cultural values and this has implications for the treatment of blushing anxieties.

Two cases of onychomycosis due to Candida zeylanoides.

As the range of yeasts and filamentous fungi reported to cause onychomycosis has increased, uncommon genera or species have appeared in the literature. Candida zeylanoides is an example of a yeast rarely involved in human pathology. Between 1989 and 1991, two cases of onychomycosis due to this yeast were encountered in Wollongong, NSW. There were similarities in the cases, as both involved finger- or thumb-nails and both patients were female (one sixty years of age and the other, seventy-two). Evidence of pathogenicity is presented and subsequent topical treatment was successful in both cases.

Upper airway obstruction in neurofibromatosis.

A patient with von Recklinghausen's neurofibromatosis developed severe upper airway obstruction at the induction of anaesthesia and required emergency cricothyroidotomy. The cause was a neurofibroma at the base of the tongue. Features of von Recklinghausen's neurofibromatosis which may be of importance to the anaesthetist are reviewed.

A ten year survey on Trichophyton tonsurans: prevalence and strain types in the general community and in aborigines.

During a 10-year period (1976-1986), a set of 154 isolates of T. tonsurans were examined. These were derived from patients in the Illawarra, South Coast and North Coast areas of NSW. The American experience of rapid increase in T. tonsurans infections was not repeated in this survey. There appeared to be good evidence of particular strains of T. tonsurans causing infections almost exclusively in aboriginal patients (96.6%), while other strains substantially caused infections in non-aboriginal patients (78.4%).

Comparative electrophoretic examination of the two European species of angler-fish (Lophiidae): Lophius piscatorius (L.) and Lophius budegassa (Spinola) and assessment of their genetic relationship.

1. A multilocus electrophoretic examination of the genetic relationships between the two European species of angler-fish was carried out using starch gel electrophoresis. 2. At 4 gene loci fixation for different alleles separated Lophius piscatorius and L. budegassa while at an additional 5 loci, frequencies of shared alleles were significantly heterogeneous. 3. A mean genetic distance of I = 0.771 was calculated, corresponding to a mean genetic distance of D = 0.260 between these species. 4. This research identifies 7 polymorphic loci in L. piscatorius and 5 in L. budegassa which will potentially serve as genetic markers for examination of population structure.

Shyness as a dimension of personality.

Examination of a number of major studies of personality questionnaires reveals the existence of a shyness factor which is related to but separable from both introversion and neuroticism, and which loads on items referring to feeling uncomfortable and self-conscious, and keeping in the background in certain kinds of social situations. The fears that are generally expressed about social situations--of being negatively evaluated and of being socially inadequate--are reflected in subjects' descriptions of the causes of shyness, and these findings suggest a model of shyness in terms of individual differences in susceptibility to threat.

An analysis of verbal-performance intelligence quotient discrepancies in the Wechsler Adult Intelligence Scale results of mentally subnormal hospital patients.

Verbal-Performance IQ discrepancies on the WAIS have been of much interest to clinical psychologists. They have been related, amongst other things, to different types of brain damage and in studies of the mentally subnormal, to successful adjustment to the outside world. This paper looks more closely at such discrepancies obtained by patients in a hospital for the mentally subnormal. These discrepancies are found to be both frequent related to Full Scale IQ. The pattern that emerges remains constant if scaled scores are considered or if the subtests are re-arranged according to Cohen's A and B factors. It is argued that these findings imply caution in the use of the discrepancy measure as a diagnostic or predictive indicator in this population.