RESUMO
Nitric oxide (NO) produced by the constitutive NO synthase (cNOS) in neurons has been implicated in mediating excitotoxic neuronal death. In our murine cortical cell culture system, NMDA neurotoxicity was not blocked by addition of the NOS inhibitors, NG-nitro-L-arginine or aminoguanidine. However, following activation of inducible NOS in astrocytes by interleukin-1 beta plus interferon-gamma, NMDA but not kainate neurotoxicity was markedly potentiated. This selective potentiation of NMDA neurotoxicity was blocked by NOS inhibition or antioxidants (superoxide dismutase/catalase or Tempol) and could be mimicked by NO generators (SIN-1 or SNAP) or the oxygen radical generator, pyragallol. These results raise the possibility that NO production by astrocytes may contribute to NMDA receptor-mediated neuronal death, perhaps through interaction with oxygen radicals.
Assuntos
Aminoácido Oxirredutases/fisiologia , Astrócitos/enzimologia , Neurônios/efeitos dos fármacos , Animais , Morte Celular/efeitos dos fármacos , Células Cultivadas , Sinergismo Farmacológico , Indução Enzimática , Técnicas In Vitro , Interferon gama/farmacologia , Interleucina-1/farmacologia , Ácido Caínico/toxicidade , Camundongos , Molsidomina/análogos & derivados , Molsidomina/farmacologia , N-Metilaspartato/toxicidade , Óxido Nítrico/fisiologia , Óxido Nítrico Sintase , Penicilamina/análogos & derivados , Penicilamina/farmacologia , S-Nitroso-N-AcetilpenicilaminaRESUMO
BACKGROUND: Intracerebroventricular (ICV) administration of kainic acid to rats produces limbic-cortical neuronal damage that has been compared to the neuropathology of schizophrenia. METHODS: Groups of adult rats were administered ICV kainic acid and then assessed for neuronal loss and the expression of proteins relevant to mechanisms of neuronal damage after one and fourteen days. Neuronal loss was assessed by two-dimensional cell counting and protein expression was assessed by immunohistochemistry. RESULTS: ICV kainic acid administration was associated with both immediate (day 1) and delayed (day 14) neuronal loss in the dorsal hippocampus. The immediate injury was largely limited to the CA3 hippocampal subfield, while the delayed injury included the CA1 subfield. Multiple mechanisms of cell death appeared to be involved in the delayed neuronal loss, as evidenced by changes in the expression of glutamate receptor subunits, heat shock protein and jun protein. CONCLUSIONS: ICV kainic acid administration to adult rats produces progressive damage to limbic-cortical neurons, involving both fast and slow mechanisms of cell death. Given the evidence for clinical deterioration, cognitive deficits and hippocampal neuropathy in some cases of schizophrenia, this animal model may be relevant for hypotheses regarding mechanisms of neurodegeneration in that disorder.
Assuntos
Ventrículos Cerebrais/efeitos dos fármacos , Ácido Caínico/efeitos adversos , Ácido Caínico/farmacocinética , Degeneração Neural/induzido quimicamente , Esquizofrenia/etiologia , Animais , Transtornos Cognitivos/induzido quimicamente , Hipocampo/efeitos dos fármacos , Sistema Límbico/efeitos dos fármacos , Masculino , Ratos , Fatores de TempoRESUMO
Injection of ferric chloride (FC) into the left amygdala of rats produced limbic seizures that lasted at least 3 weeks. In addition, FC-injected animals demonstrated motor impairment, decreased protesting vocalizations, and spontaneous stereotypies during a behavioral examination. An increase in apomorphine-induced stereotypies was also noted, and weekly administration of apomorphine for 3 weeks potentiated the increase in stereotypies produced by FC injection. These behavioral changes were associated with changes in postsynaptic dopamine D2 receptors. In animals injected only with FC, an increase in the [3H]-spiperone Bmax in the left nucleus accumbens and an increase in Kd in the right nucleus accumbens were noted. In FC-injected animals challenged weekly with apomorphine for 3 weeks, increases in the [3H]-spiperone Bmax in both amygdalae, the left nucleus accumbens, and the right nucleus caudatus and increases in Kd in the left amygdala and right nucleus accumbens were noted. Severance of the anterior commissure at the time of FC injection reversed most of these changes in behavior and dopamine receptor binding. Possible mechanisms for these changes are discussed, as well as the implications of these results for research on limbic dysfunction and psychopathology.
Assuntos
Tonsila do Cerebelo/efeitos dos fármacos , Compostos Férricos/farmacologia , Ferro/farmacologia , Receptores Dopaminérgicos/efeitos dos fármacos , Convulsões/induzido quimicamente , Agressão/efeitos dos fármacos , Tonsila do Cerebelo/metabolismo , Animais , Apomorfina/farmacologia , Comportamento Animal/efeitos dos fármacos , Cloretos , Humanos , Cinética , Ratos , Receptores Dopaminérgicos/metabolismo , Espiperona/metabolismo , Comportamento Estereotipado/efeitos dos fármacosRESUMO
The temporal evolution of cerebral infarction was examined in rats subjected to transient occlusion of both common carotid arteries and the right middle cerebral artery. After severe (90-min) ischemia, substantial right-sided cortical infarction was evident within 6 h and fully developed after 1 day. After mild (30-min) ischemia, no cortical infarction was present after 1 day. However, infarction developed after 3 days; by 2 weeks, infarction volume was as large as that induced by 90-min ischemia. These data suggest that infarction after mild focal ischemia can develop in a surprisingly delayed fashion. Some evidence of neuronal apoptosis was present after severe ischemia, but only to a limited degree. However, 3 days after mild ischemia, neurons bordering the maturing infarction exhibited prominent TUNEL staining, and DNA prepared from the periinfarct area of ischemic cortex showed internucleosomal fragmentation. Furthermore, pretreatment with 1 mg/kg cycloheximide markedly reduced infarction volume 2 weeks after mild ischemia. These data raise the possibility that apoptosis, dependent on active protein synthesis, contributes to the delayed infarction observed in rats subjected to mild transient focal cerebral ischemia.
Assuntos
Apoptose , Córtex Cerebral/patologia , Infarto Cerebral/etiologia , Ataque Isquêmico Transitório/complicações , Neurônios/patologia , Animais , Artérias Carótidas , Artérias Cerebrais , Córtex Cerebral/metabolismo , Infarto Cerebral/patologia , Constrição , Cicloeximida/farmacologia , DNA/metabolismo , Masculino , Ratos , Coloração e Rotulagem , Fatores de TempoRESUMO
Tg2576 transgenic mice (mice overexpressing the "Swedish" mutation in the human amyloid precursor protein 695) demonstrated a decreased capacity for cell proliferation in the dentate gyrus of the hippocampus compared with non-transgenic littermates at 3 months, 6 months and 9 months of age. Isolation stress induced by individually housing each mouse from the time of weaning further decreased hippocampal cell proliferation in Tg2576 mice as well as in non-transgenic littermates at 6 months of age. Decreases in hippocampal cell proliferation in isolated Tg2576 mice were associated with impairments in contextual but not cued memory. Fluoxetine administration increased cell proliferation and improved contextual memory in isolated Tg2576 mice. Further, isolation stress accelerated the age-dependent deposition of beta-amyloid 42 plaques in Tg2576 mice. Numerous beta-amyloid plaques were found in isolated but not non-isolated Tg2576 mice at 6 months of age. These results suggest that Tg2576 mice, a mouse model of Alzheimer disease, have an impaired ability to generate new cells in the dentate gyrus of the hippocampus and that the magnitude of this impairment can be modulated by behavioral interventions and drugs known to have effects on hippocampal neurogenesis in normal rodents. Unexpectedly, isolation stress also appeared to accelerate the underlying process of beta-amyloid plaque deposition in Tg2576 mice. These results suggest that stress may have an impact on the underlying disease process associated with Alzheimer's disease.
Assuntos
Precursor de Proteína beta-Amiloide/genética , Amiloidose/patologia , Amiloidose/fisiopatologia , Giro Denteado/patologia , Giro Denteado/fisiopatologia , Memória/fisiologia , Animais , Divisão Celular , Giro Denteado/efeitos dos fármacos , Modelos Animais de Doenças , Fluoxetina/farmacologia , Memória/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Inibidores Seletivos de Recaptação de Serotonina/farmacologia , Isolamento Social , Estresse Psicológico/tratamento farmacológico , Estresse Psicológico/patologia , Estresse Psicológico/fisiopatologiaRESUMO
Antipsychotic drugs increase expression of the immediate early gene, c-fos, in the striatum, nucleus accumbens and prefrontal cortex of rat brain. Since intracerebro-ventricular (i.c.v.) infusion of kainic acid (KA) produces loss of limbic-cortical neurons that project to these brain areas, we postulated that the c-fos responses to antipsychotics in these brain areas would be altered following i.c.v. KA administration. To produce limbic-cortical lesions, rats received i.c.v. infusions of either KA (4.5 nmol) or vehicle. Then, 25 28 days later, rats received 0.13, 0.35, or 1.5 mg/kg haloperidol, 6.3, 17.5, or 30.0 mg/kg clozapine, or saline. In both KA-lesioned and control animals, haloperidol produced greater increases in Fos protein immunoreactivity in the striatum than in limbic-cortical areas, while clozapine produced greater increases in Fos protein immunoreactivity in limbic-cortical areas than in the striatum. In both KA-lesioned and control animals, haloperidol and clozapine administration also produced similar dose-dependent increases in Fos protein immunoreactivity in the striatum and nucleus accumbens. However, the ability of clozapine to increase Fos protein immunoreactivity in the infralimbic prefrontal cortex was significantly enhanced in KA-lesioned rats compared to controls. Since limbic-cortical pathology has been implicated in the negative symptoms of schizophrenia, the enhanced effect of clozapine on limbic-cortical expression of c-fos in KA-lesioned rats may be relevant to understanding clozapine's unusual therapeutic actions in patients with schizophrenia.
Assuntos
Antipsicóticos/farmacologia , Encéfalo/efeitos dos fármacos , Expressão Gênica/efeitos dos fármacos , Sistema Límbico/efeitos dos fármacos , Proteínas Proto-Oncogênicas c-fos/biossíntese , Animais , Encéfalo/metabolismo , Encefalopatias/induzido quimicamente , Encefalopatias/metabolismo , Encefalopatias/patologia , Contagem de Células , Clozapina/administração & dosagem , Clozapina/farmacologia , Relação Dose-Resposta a Droga , Genes fos/efeitos dos fármacos , Haloperidol/administração & dosagem , Haloperidol/farmacologia , Ácido Caínico/farmacologia , Sistema Límbico/metabolismo , Sistema Límbico/patologia , Masculino , Neurônios/efeitos dos fármacos , Neurônios/metabolismo , Proteínas Proto-Oncogênicas c-fos/genética , Proteínas Proto-Oncogênicas c-fos/metabolismo , Ratos , Ratos Sprague-DawleyRESUMO
Ingestion of 3-nitropropionic acid (3-NPA) in moldy sugar cane causes brain damage in children. The mechanism of 3-NPA toxicity is thought to be inhibition of energy production, leading to ATP depletion and excitotoxicity. We exposed cultured mouse striatal or cortical neurons to 1-2 mM 3-NPA for 48 h. This exposure produced gradual neuronal degeneration characterized by cell body shrinkage and DNA fragmentation. Addition of glutamate antagonists during 3-NPA exposure did not reduce neuronal death. However, addition of the macromolecular synthesis inhibitors cycloheximide, emetine or actinomycin D markedly reduced neuronal death. Our results do not exclude that 3-NPA can induce excitotoxicity in more intact systems, but raise the additional possibility that 3-NPA may also act to induce neuronal apoptosis.
Assuntos
Apoptose , Propionatos/farmacologia , Córtex Visual/efeitos dos fármacos , Animais , Cálcio , Morte Celular , Células Cultivadas , Camundongos , Degeneração Neural , Neurônios/efeitos dos fármacosRESUMO
Previous studies have implicated both excitotoxicity and apoptosis in the pathogenesis of cerebral infarction induced by focal ischemic insults. Here we tested the possibility that the NMDA antagonist, dextrorphan, and the protein synthesis inhibitor, cycloheximide, would produce additive protective effects in a rodent model of focal ischemia-reperfusion. Transient focal cerebral ischemia was induced by a 90 min period of ligation of the right middle cerebral artery and both common carotid arteries. Administration of either 30 mg/kg dextrorphan or 0.5 mg/kg cycloheximide, given i.p. 15 min before ischemia, reduced infarct volume by about 65%. When optimal concentrations of each drug were given together, infarct volume was reduced by 87% as measured 14 days later. These observations support the idea that both excitotoxicity, and apoptosis dependent on new protein synthesis, contribute to cerebral infarction after transient focal ischemia in the rat.
Assuntos
Cicloeximida/farmacologia , Dextrorfano/farmacologia , Ataque Isquêmico Transitório/patologia , Fármacos Neuroprotetores/farmacologia , Inibidores da Síntese de Proteínas/farmacologia , Animais , Glicemia/metabolismo , Artérias Cerebrais/fisiologia , Infarto Cerebral/patologia , Masculino , RatosRESUMO
Excitotoxins, such as kainic acid (KA), have been shown to produce both immediate and delayed neuronal degeneration in adult rat brain. While preweanling rats have been shown to be resistant to the immediate neurotoxicity of KA, the presence of delayed neuronal loss has not been investigated in such animals. To determine whether intracerebroventricular (i.c.v.) administration of KA would produce delayed neuronal loss, preweanling rats were administered 5 nmol or 10 nmol KA i.c.v. on postnatal day 7 (P7) and then examined at P14, P45, and P75. Using three-dimensional, non-biased cell counting, neuronal loss was observed in the CA3 subfield of the hippocampal formation at P45 and P75 in animals administered 10 nmol KA, as compared to animals administered 5 nmol KA or artificial cerebrospinal fluid. Further, the amount of immunoreactivity to jun, the protein product of the immediate early gene, c-jun, adjusted for the number of remaining neurons was increased in the same brain areas. Antibody labeling of inducible heat shock protein and glial fibrillary acidic protein was not similarly increased in animals administered i.c.v. KA. The data suggest that while i.c.v. KA does not produce immediate neuronal loss in preweanling rats, the hippocampus is altered so that neuronal loss occurs after a delay, perhaps through apoptosis. These findings may be relevant to the pathogenesis of neuropsychiatric disorders, such as schizophrenia, that are characterized by early limbic-cortical deficits but onset of illness in young adulthood.
Assuntos
Animais Lactentes/fisiologia , Hipocampo/efeitos dos fármacos , Hipocampo/patologia , Ácido Caínico/farmacologia , Neurônios/efeitos dos fármacos , Envelhecimento/fisiologia , Animais , Animais Lactentes/crescimento & desenvolvimento , Contagem de Células/efeitos dos fármacos , Feminino , Proteína Glial Fibrilar Ácida/metabolismo , Proteínas de Choque Térmico HSP70/metabolismo , Hipocampo/crescimento & desenvolvimento , Hipocampo/metabolismo , Imuno-Histoquímica , Injeções Intraventriculares , Masculino , Neurônios/patologia , Proteínas Proto-Oncogênicas c-jun/metabolismo , Ratos , Ratos Sprague-Dawley , Fatores de TempoRESUMO
Isolation and intermittent oscillation produced distinct effects on the subsequent stereotypic response to apomorphine in rats. These effects also differed from the effects of intermittent apomorphine and chronic haloperidol pre-treatment. Varying the frequency of oscillation stress changed the magnitude but not the type of the effect. Thus, different types of stress may effect the dopaminergic neurochemical system uniquely.
Assuntos
Apomorfina/farmacologia , Haloperidol/farmacologia , Isolamento Social , Comportamento Estereotipado/efeitos dos fármacos , Estresse Psicológico/fisiopatologia , Animais , Humanos , Masculino , Ratos , Ratos Endogâmicos , Fatores de TempoAssuntos
Tonsila do Cerebelo/efeitos dos fármacos , Carbamazepina/farmacologia , Compostos Férricos/antagonistas & inibidores , Ferro/antagonistas & inibidores , Receptores Dopaminérgicos/efeitos dos fármacos , Animais , Apomorfina/farmacologia , Cloretos , Compostos Férricos/farmacologia , Ratos , Ratos Endogâmicos , Comportamento Estereotipado/efeitos dos fármacosRESUMO
A centrifugation method for the separation of bound and unbound ligand during 3[H]-spiroperidol binding in rat brain was compared with the more widely used filtration method. The centrifugation method yielded significantly greater total and specific binding, and lower nonspecific binding, than filtration. Assay reproducibility was also found to be superior when the centrifugation method was used as compared to the filtration binding method. Scatchard plots produced by both methods yielded comparable Kd and Bmax values that were consistent with those previously reported.
Assuntos
Encéfalo/metabolismo , Butirofenonas/metabolismo , Espiperona/metabolismo , Animais , Centrifugação , Corpo Estriado/metabolismo , Filtração , Técnicas In Vitro , Cinética , Ligantes , Masculino , Núcleo Accumbens/metabolismo , Ratos , Ratos EndogâmicosRESUMO
The effect of a matrix of concentrations of Ca2+ (0.01, 0.1, 0.5, 5 mM), Mg2+ (0.2, 0.5, 1, 2, 5, 10 mM), and Na+ (50, 100, 150 mM) on the phosphorylation of histone H-1 by protein kinase C was measured in the presence of 5 mol % diacylglycerol and Mg-ATP in both phosphatidylserine micelles and liposomes formed from a 1:4 mixture of phosphatidylserine and phosphatidylcholine. Monovalent cations (150 mM) reduced activity by 60 and 84% in the micelle and liposome assay systems, respectively. Inhibition was also observed with 5 mM Ca2+ and 10 mM Mg2+. The phosphorylating activity was compared with computer calculations of the negative electrostatic potentials (psi o) of the phospholipid membranes in the presence of the cations.
Assuntos
Cálcio/farmacologia , Magnésio/farmacologia , Potássio/farmacologia , Proteína Quinase C/antagonistas & inibidores , Animais , Bovinos , Lipossomos , Micelas , Concentração Osmolar , Fosfatidilserinas , Fosforilação , Fosforilcolina , Proteína Quinase C/metabolismo , RatosRESUMO
3[H]-(-)-Sulpiride and 3[H]-spiperone binding was compared in rat amygdala, nucleus accumbens and striatum, using (+/-)-sulpiride to define specific binding. 3[H]-(-)-Sulpiride bound to twice as many sites in amygdala and nucleus accumbens as 3[H]-spiperone. 3[H]-(-)-Sulpiride binding was directed to these additional sites by using 1 microM spiperone to mask dopaminergic binding. The binding of 3[H]-(-)-sulpiride to these sites was high affinity, reversible, Na+-dependent, but not stereospecific. Metoclopramide, tiapride and antidepressant medications, but not other neuroleptics, ADTN, or serotonin displaced 3[H]-(-)-sulpiride binding to these sites. These data suggest that 3[H]-(-)-sulpiride labels mesolimbic sites other than dopamine receptors which may mediate antidepressant effects.
Assuntos
Tonsila do Cerebelo/metabolismo , Antidepressivos/metabolismo , Corpo Estriado/metabolismo , Núcleo Accumbens/metabolismo , Núcleos Septais/metabolismo , Sulpirida/metabolismo , Animais , Sítios de Ligação , Ligação Competitiva , Cinética , Masculino , Metoclopramida/metabolismo , Ratos , Espiperona/metabolismo , Cloridrato de Tiapamil/metabolismoRESUMO
The non-specific endonuclease inhibitor, aurintricarboxylic acid (ATA), attenuated glutamate-induced destruction of cultured cortical neurons. In part, this protective effect likely reflected the ability of ATA to produce a slowly developing block of N-methyl-D-aspartate receptor-mediated inward whole cell current or increase in intracellular free Ca2+. However, ATA also attenuated a high K(+)-induced increase in intracellular free Ca2+ in the presence of D-amino-phosphonovalerate, suggesting that ATA may have a more general effect on Ca2+ homeostasis. In addition, ATA attenuated glutamate neurotoxicity even if added up to 2 hr after completion of glutamate exposure, a time when glutamate antagonists or lipid peroxidation inhibitors are no longer neuroprotective. Involvement of apoptosis in this excitotoxic death is unlikely, as Southern blotting of genomic DNA revealed no evidence of fragmentation, and death was not prevented by inhibitors of RNA or protein synthesis. Most likely, ATA interferes with some key downstream consequences of excitotoxic glutamate receptor overactivation.