RESUMO
Recent advances highlight the versatility and complexity of this highly conserved axon and cell migration guidance system. Characterization of netrin mutant phenotypes in worm, fly and mouse all suggest that netrins play local as well as long-range roles in guidance. Evidence from multiple sources also indicates that the netrin receptor DCC can mediate both attractive and repulsive responses to netrins.
Assuntos
Proteínas de Caenorhabditis elegans , Movimento Celular/fisiologia , Cones de Crescimento/fisiologia , Fatores de Crescimento Neural , Sistema Nervoso/crescimento & desenvolvimento , Receptores de Fator de Crescimento Neural , Moléculas de Adesão CelularRESUMO
The nematode Caenorhabditis elegans should be an excellent model system in which to study the role of microtubules in mitosis, embryogenesis, morphogenesis, and nerve function. It may be studied by the use of biochemical, genetic, molecular biological, and cell biological approaches. We have purified microtubules and microtubule-associated proteins (MAPs) from C. elegans by the use of the anti-tumor drug taxol (Vallee, R. B., 1982, J. Cell Biol., 92:435-44). Approximately 0.2 mg of microtubules and 0.03 mg of MAPs were isolated from each gram of C. elegans. The C. elegans microtubules were smaller in diameter than bovine microtubules assembled in vitro in the same buffer. They contained primarily 9-11 protofilaments, while the bovine microtubules contained 13 protofilaments. The principal MAP had an apparent molecular weight of 32,000 and the minor MAPs were 30,000, 45,000, 47,000, 50,000, 57,000, and 100,000-110,000 mol wt as determined by SDS-gel electrophoresis. The microtubules were observed, by electron microscopy of negatively stained preparations, to be connected by stretches of highly periodic cross-links. The cross-links connected the adjacent protofilaments of aligned microtubules, and occurred at a frequency of one cross-link every 7.7 +/- 0.9 nm, or one cross-link per tubulin dimer along the protofilament. The cross-links were removed when the MAPs were extracted from the microtubules with 0.4 M NaCl. The cross-links then re-formed when the microtubules and the MAPs were recombined in a low salt buffer. These results strongly suggest that the cross-links are composed of MAPs.
Assuntos
Caenorhabditis/ultraestrutura , Proteínas Associadas aos Microtúbulos/metabolismo , Microtúbulos/ultraestrutura , Animais , Microscopia Eletrônica , Proteínas dos Microtúbulos/análise , Microtúbulos/metabolismo , Peso Molecular , Inibidores de Proteases/farmacologiaRESUMO
The unc-129 gene, like the unc-6 netrin gene, is required to guide pioneer motoraxons along the dorsoventral axis of Caenorhabditis elegans. unc-129 encodes a member of the transforming growth factor-beta (TGF-beta) superfamily of secreted signaling molecules and is expressed in dorsal, but not ventral, rows of body wall muscles. Ectopic expression of UNC-129 from ventral body wall muscle disrupts growth cone and cell migrations that normally occur along the dorsoventral axis. Thus, UNC-129 mediates expression of dorsoventral polarity information required for axon guidance and guided cell migrations in C. elegans.
Assuntos
Axônios/fisiologia , Proteínas de Caenorhabditis elegans , Caenorhabditis elegans/fisiologia , Proteínas de Helminto/genética , Proteínas de Helminto/fisiologia , Músculos/metabolismo , Proteínas do Tecido Nervoso , Receptores de Superfície Celular , Fator de Crescimento Transformador beta/genética , Fator de Crescimento Transformador beta/fisiologia , Sequência de Aminoácidos , Animais , Padronização Corporal , Caenorhabditis elegans/genética , Caenorhabditis elegans/crescimento & desenvolvimento , Movimento Celular , Expressão Gênica , Proteínas de Helminto/química , Proteínas de Membrana/genética , Proteínas de Membrana/fisiologia , Dados de Sequência Molecular , Neurônios Motores/fisiologia , Netrinas , Regiões Promotoras Genéticas , Receptores de Fatores de Crescimento/genética , Receptores de Fatores de Crescimento/fisiologia , Deleção de Sequência , Transdução de Sinais , Fator de Crescimento Transformador beta/químicaRESUMO
Three known genes guide circumferential migrations of pioneer axons and mesodermal cells on the nematode body wall. unc-5 affects dorsal migrations, unc-40 primarily affects ventral migrations, and unc-6 affects migrations in both directions. Circumferential movements still occur, but are misdirected whereas longitudinal movements are normal in these mutants. Pioneer growth cones migrating directly on the epidermis are affected; growth cones migrating along established axon fascicles are normal. Thus these genes affect cell guidance and not cell motility per se. We propose that two opposite, adhesive gradients guide circumferential migrations on the epidermis. unc-5, unc-6, and unc-40 may encode these adhesion molecules or their cellular receptors. Neurons have access to the basal lamina and the basolateral surfaces of the epidermis, but mesodermal cells contact only the basal lamina. These genes probably identify molecular cues on the basal lamina that guide mesodermal migrations. The same basal lamina cues, or perhaps related molecules on the epidermal cell surfaces, guide pioneer neurons.
Assuntos
Axônios/fisiologia , Células Epidérmicas , Genes/fisiologia , Animais , Divisão Celular , Movimento Celular , Músculos/citologia , Músculos/embriologia , Músculos/fisiologia , Nematoides , Sistema Nervoso/embriologia , FenótipoRESUMO
The unc-6 gene is required for the guidance of pioneer axons and migrating cells along the body wall in C. elegans. In mutants, dorsal and ventral migrations are disrupted, but longitudinal movements are largely unaffected. The gene was tagged for molecular cloning by two independent transposon insertions. Based on genomic and cDNA sequencing, the gene encodes a novel laminin-related protein, UNC-6 (591 amino acids). The N-terminus is homologous to the N-termini (i.e., domains V1, V-1, V-2, and V-3) of laminin subunits, while the C-terminus is a unique domain. We propose that UNC-6 is a component of an extracellular matrix cue that guides dorsoventral migrations on the epidermis.
Assuntos
Axônios/fisiologia , Proteínas de Caenorhabditis elegans , Caenorhabditis elegans/citologia , Movimento Celular/fisiologia , Proteínas de Helminto/metabolismo , Laminina/química , Proteínas do Tecido Nervoso , Sequência de Aminoácidos , Animais , Sequência de Bases , Southern Blotting , Clonagem Molecular , DNA/química , DNA/genética , Elementos de DNA Transponíveis , Éxons , Proteínas de Helminto/química , Proteínas de Helminto/genética , Dados de Sequência Molecular , Mutação , Netrinas , RNA Mensageiro/química , Homologia de Sequência de AminoácidosRESUMO
Robo receptors interact with ligands of the Slit family. The nematode C. elegans has one Robo receptor (SAX-3) and one Slit protein (SLT-1), which direct ventral axon guidance and guidance at the midline. In larvae, slt-1 expression in dorsal muscles repels axons to promote ventral guidance. SLT-1 acts through the SAX-3 receptor, in parallel with the ventral attractant UNC-6 (Netrin). Removing both UNC-6 and SLT-1 eliminates all ventral guidance information for some axons, revealing an underlying longitudinal guidance pathway. In the embryo, slt-1 is expressed at high levels in anterior epidermis. Embryonic expression of SLT-1 provides anterior-posterior guidance information to migrating CAN neurons. Surprisingly, slt-1 mutants do not exhibit the nerve ring and epithelial defects of sax-3 mutants, suggesting that SAX-3 has both Slit-dependent and Slit-independent functions in development.
Assuntos
Axônios/fisiologia , Proteínas de Caenorhabditis elegans , Proteínas do Tecido Nervoso/genética , Proteínas do Tecido Nervoso/metabolismo , Receptores Imunológicos/genética , Receptores Imunológicos/metabolismo , Animais , Caenorhabditis elegans , Movimento Celular , Regulação da Expressão Gênica no Desenvolvimento , Proteínas de Fluorescência Verde , Proteínas de Helminto/genética , Proteínas de Helminto/metabolismo , Indicadores e Reagentes/metabolismo , Proteínas Luminescentes/genética , Músculos/inervação , Músculos/fisiologia , Mutagênese/fisiologia , Proteínas do Tecido Nervoso/química , Netrinas , Neurônios/fisiologia , Neurônios/ultraestrutura , Estrutura Terciária de Proteína , Proteínas RoundaboutRESUMO
Genetic studies have identified an extracellular path cue molecule, UNC-6, and a neuronal receptor, UNC-5, that act to guide migrating pioneer growth cones along the dorsoventral coordinate of the Caenorhabditis elegans body wall. Ectopic expression studies and characterization of mutants have demonstrated directly the instructive action of these molecules, suggesting a molecular model for how they perform their guidance functions. Recent evidence suggests that these and other genetically identified axon guidance molecules are likely to have vertebrate counterparts.
Assuntos
Axônios/fisiologia , Proteínas de Caenorhabditis elegans , Caenorhabditis elegans/embriologia , Regulação da Expressão Gênica no Desenvolvimento , Proteínas de Helminto/fisiologia , Proteínas de Membrana/fisiologia , Proteínas do Tecido Nervoso , Receptores de Superfície Celular , Receptores de Fatores de Crescimento/fisiologia , Animais , Caenorhabditis elegans/genética , Movimento Celular/genética , Polaridade Celular/genética , Fatores Quimiotáticos/genética , Embrião não Mamífero/fisiologia , Proteínas de Helminto/genética , Proteínas de Helminto/metabolismo , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Mutação , Fatores de Crescimento Neural/genética , Netrinas , Vias Neurais/embriologia , Receptores de Fatores de Crescimento/genética , Receptores de Fatores de Crescimento/metabolismo , TropismoRESUMO
Neuronal growth cones respond to both contact-mediated and chemotropic guidance cues; these cues can be either attractive or repulsive. This past year has seen further characterization of two gene families implicated in long-range chemoattraction and chemorepulsion: the netrins and the semaphorins. Analysis of invertebrate members of these gene families demonstrates in vivo how netrins play multiple roles in axonal guidance in Caenorhabditis elegans, how specific domains of the netrin molecule confer attractive and repulsive guidance cues, and how semaphorins can function to generate neuromuscular specificity.
Assuntos
Axônios/fisiologia , Sistema Nervoso/crescimento & desenvolvimento , Animais , Sinais (Psicologia) , Transdução de Sinais/fisiologiaRESUMO
A wild-type strain of the nematode Caenorhabditis elegans has been shown to avoid high concentrations of a number of sugars and salts. Individual and population assays for this response were developed and mutants were selected for their inability to avoid high concentrations of fructose or NaCl, Seven nonavoiding mutants representing six complementation groups were isolated and characterized. Genetic studies indicate that the mutants each carry a single recessive mutation responsible for the defective osmotic avoidance behavior. The map locations of the six complementation groups identified by these mutations have been determined. Mutants isolated for their inability to avoid fructose are also unable to avoid NaCl and vice versa. The mutants move normally, exhibit normal touch sensitivity, and, like wild type, follow isotherms in a radial thermal gradient. All of the mutants are at least partially defective in the attraction to sodium chloride exhibited by wild type. None of the mutants is temperature sensitive, and all exhibit defective osmotic avoidance behavior as young L1 larvae. Preliminary anatomical studies indicate selective sensory neuron changes in at least one mutant.
Assuntos
Mutação , Nematoides/genética , Concentração Osmolar , Animais , Genes Recessivos , FenótipoRESUMO
Cell and growth cone migrations along the dorsoventral axis of Caenorhabditis elegans are mediated by the UNC-5 and UNC-40 receptor subtypes for the secreted UNC-6 guidance cue. To characterize UNC-6 receptor function in vivo, we have examined genetic interactions between unc-5 and unc-40 in the migrations of the hermaphrodite distal tip cells. We report that cell migration defects as severe as those associated with a null mutation in unc-6 are produced only by null mutations in both unc-5 and unc-40, indicating that either receptor retains some partial function in the absence of the other. We show that hypomorphic unc-5 alleles exhibit two distinct types of interallelic genetic interactions. In an unc-40 wild-type genetic background, some pairs of hypomorphic unc-5 alleles exhibit a partial allelic complementation. In an unc-40 null background, however, we observed that unc-5 hypomorphs exhibit dominant negative effects. We propose that the UNC-5 and UNC-40 netrin receptors can function to mediate chemorepulsion in DTC migrations either independently or together, and the observed genetic interactions suggest that this flexibility in modes of signaling results from the formation of a variety of oligomeric receptor complexes.
Assuntos
Proteínas de Caenorhabditis elegans , Caenorhabditis elegans/metabolismo , Moléculas de Adesão Celular/metabolismo , Proteínas de Helminto/metabolismo , Proteínas de Membrana/metabolismo , Proteínas do Tecido Nervoso , Receptores de Superfície Celular , Receptores de Fatores de Crescimento/metabolismo , Transdução de Sinais , Animais , Animais Geneticamente Modificados , Caenorhabditis elegans/genética , Moléculas de Adesão Celular/genética , Proteínas de Helminto/genética , Proteínas de Membrana/genética , Netrinas , Polimorfismo Conformacional de Fita Simples , Receptores de Fatores de Crescimento/genéticaRESUMO
The unc-73 gene of Caenorhabditis elegans is necessary for proper axon guidance. Animals mutant in this gene are severely uncoordinated and also exhibit defects in cell migration and cell lineages. We have isolated coordinated revertants of unc-73 (e936). These fall into three classes: intragenic revertants, extragenic dominant suppressors (sup-39), and a single apparently intragenic mutation that is a dominant suppressor with a linked recessive lethal phenotype. sup-39 mutations cause early embryonic lethality, but escapers have a wild-type movement phenotype as larvae and adults. Gonads of sup-39 mutant animals show a novel defect: normal gonads have a single row of oocytes, but sup-39 gonads often have two rows of oocytes. This result suggests that the mutant gonad is defective in choosing on its surface only a single site form which nuclei will emerge to form oocytes. These results are interpreted in terms of an effect of unc-73 on determination of cell polarity.
Assuntos
Caenorhabditis elegans/genética , Genes de Helmintos , Genes Supressores , Supressão Genética , Alelos , Sequência de Aminoácidos , Animais , Sequência de Bases , Caenorhabditis elegans/fisiologia , Proteínas de Caenorhabditis elegans , Proteínas de Ciclo Celular/química , Proteínas de Ciclo Celular/genética , Mapeamento Cromossômico , Primers do DNA , Transtornos do Desenvolvimento Sexual/genética , Embrião não Mamífero , Feminino , Proteínas de Ligação ao GTP/química , Proteínas de Ligação ao GTP/genética , Genes Dominantes , Genes Letais , Marcadores Genéticos , Genótipo , Íntrons , Larva , Masculino , Dados de Sequência Molecular , Movimento , Mutagênese , Oócitos/citologia , Oócitos/fisiologia , Fenótipo , Reação em Cadeia da PolimeraseRESUMO
In JOHNSON et al. (1981), the Caenorhabditis elegans mutant strain PR1000, homozygous for the ace-1 mutation p1000, is shown to be deficient in the class A subset of acetylcholinesterases, which comprises approximately one-half of the total C. elegans acetylcholinesterase activity. Beginning with this strain, we have isolated 487 new behavioral and morphological mutant strains. Two of these, independently derived, lack approximately 98% of the wild-type acetylcholinesterase activity and share the same specific uncoordinated phenotype; both move forward in a slow and uncoordinated manner, and when mechanically stimulated to induce reversal, both hypercontract and become temporarily paralyzed. In addition to the ace-1 mutation, both strains also harbor recessive mutations in the same newly identified gene, ace-2, which maps to chromosome I and is therefore not linked to ace-1. Gene dosage experiments suggest that ace-2 is a structural gene for the remaining class B acetylcholinesterases, which are not affected by ace-1.--The uncoordinated phenotype of the newly isolated, doubly mutant strains depends on both the ace-1 and ace-2 mutations; homozygosity for either mutation alone produces normally coordinated animals. This result implies functional overlap of the acetylcholinesterases controlled by ace-1 and ace-2, perhaps at common synapses. Consistent with this, light microscopic histochemical staining of permeabilized whole mounts indicates some areas of possible spatial overlap of these acetylcholinesterases (nerve ring, longitudinal nerve cords). In addition, there is at least one area where only ace-2-controlled acetylcholinesterase activity appears (pharyngeo-intestinal valve).
Assuntos
Acetilcolinesterase/genética , Caenorhabditis/genética , Genes , Mutação , Animais , Comportamento Animal , Locomoção , FenótipoRESUMO
Within a set of five separable molecular forms of acetylcholinesterase found in the nematode Caenorhabditis elegans, previously reported differences in kinetic properties identify two classes, A and B, likely to be under separate genetic control. Using differences between these classes in sensitivity to inactivation by sodium deoxycholate, a screening procedure was devised to search for mutants affected only in class A forms. Among 171 previously isolated behavioral and morphological mutant strains examined by this procedure, one (PR946) proved to be of the expected type, exhibiting a selective deficiency of class A acetylcholinesterase forms. Although originally isolated because of its uncoordinated behavior, this strain was subsequently shown to harbor mutations in two genes; one in the previously identified gene unc-3, accounting for its behavior, and one in a newly identified gene, ace-1, accounting for its selective acetylcholinesterase deficiency. Derivatives homozygous only for the ace-1 mutation also lacked class A acetylcholinesterase forms, but were behaviorally and developmentally indistinguishable from wild type. The gene ace-1 has been mapped near the right end of the X chromosome. Gene dosage experiments suggest that it may be a structural gene for a component of class A acetylcholinesterase forms.
Assuntos
Acetilcolinesterase/genética , Caenorhabditis/genética , Mutação , Animais , Mapeamento Cromossômico , GenesRESUMO
As neuronal growth cones explore the terrain, their migration is dictated by guidance cues in the environment. Some cues are considered bifunctional because they can elicit an attractive or repulsive response. However, the cytoplasmic tails of guidance cue receptors appear to also control how growth cones respond. Merz and Culotti discuss recent evidence that the cytoplasmic domains of the DCC and UNC-5 family of receptors control the response of neurons to netrins, and propose a model for mechanisms by which axon guidance receptors might function.
Assuntos
Axônios/fisiologia , Receptores de Superfície Celular/fisiologia , Transdução de Sinais/fisiologia , Animais , Cones de Crescimento/fisiologiaRESUMO
Model organisms like Caenorhabditis elegans allow the study of growth cone motility and guidance in vivo. We are using circumferential axon guidance in C. elegans to study both the mechanisms of guidance and the interactions between different guidance systems in vivo. A genetic screen has identified suppressors of the specific axon guidance defects caused by ectopic expression of UNC-5, the repulsive receptor for the UNC-6/netrin guidance cue. These mutations identify eight genes whose products are required for the function of UNC-5 in these cells. In principle, the functions of some of these genes may involve unc-73, which encodes a multidomain, cytoplasmic protein that is an activator of the rac and rho GTPases. Loss of UNC-73 causes errors in axon guidance, and it is hypothesized that UNC-73 acts in multiple signaling pathways used by guidance receptors on the growth cone surface to regulate the underlying cytoskeleton. Here we summarize and discuss these recent developments that are advancing our understanding of growth cone signal transduction in vivo.
Assuntos
Proteínas de Caenorhabditis elegans , Caenorhabditis elegans/genética , Movimento Celular/genética , Cones de Crescimento/fisiologia , Animais , Cones de Crescimento/química , Proteínas de Helminto/genética , Mutação/fisiologia , Proteínas do Tecido Nervoso/genética , Neurônios/química , Neurônios/ultraestruturaRESUMO
Antibodies to horseradish peroxidase (HRP) recognize 27 of 302 neurons and several non-neuronal cells in adult hermaphrodites of the soil nematode Caenorhabditis elegans and can be used to label these cells for cytological analysis in whole animals. The antibodies bind to the anterior members, but not to the posterior members of a set of mechanosensory neurons in wild type animals. Binding to one of the posterior mechanosensory neurons (PVM) occurs when this neuron migrates to an abnormal anterior position in mab-5 mutant animals, suggesting that expression of the epitope recognized by these antibodies is position dependent or that mab-5 mutations transform PVM into AVM intrinsically. The antibodies were used to characterize morphologies of two pairs of lumbar neurons (PHC and PVN) in uncoordinated mutants representing 95 unc genes. PHC and PVN morphologies were normal in most of the unc mutants examined, however, in mutants of 9 unc genes (unc-6, unc-13, unc-33, unc-44, unc-51, unc-61, unc-71, unc-73, and unc-98), misdirected PHC and/or PVN processes were observed at a high frequency. The morphologies of 2 other lumbar neurons, PHA and PHB, were determined previously in these mutants (Hedgecock et al., 1985). Mutations in most, but not all of these 9 unc genes affect the growth of the embryonic lumbar neurons PHA and PHB differently than they affect the growth of the postembryonic lumbar neurons PHC and PVN, indicating that these neurons require different, but overlapping sets of genes for different stages of normal growth and guidance.
Assuntos
Caenorhabditis/genética , Mutação , Neurônios/fisiologia , Alelos , Animais , Anticorpos/isolamento & purificação , Caenorhabditis/fisiologia , Peroxidase do Rábano Silvestre/imunologia , Imuno-Histoquímica , Neurônios/citologiaRESUMO
The UNC-5 guidance receptor, in response to the UNC-6/netrin path cue, orients growing axons in a dorsal direction along the epidermis of Caenorhabditis elegans. When ectopically expressed in the touch neurons, which normally extend ventrally or longitudinally, UNC-5 is able to reorient their axons toward the dorsal side in an UNC-6-dependent manner. This forms the basis of a genetic screen to identify other mutations that, like unc-6 mutations, suppress unc-5-induced growth cone guidance. These mutations may identify new components required for pioneer axon guidance by unc-5. In this paper, we describe eight genes that are required for ectopic unc-5-induced growth cone steering. Mutations in four of these identify the previously known axon guidance genes unc-6, unc-40, unc-34, and unc-44 and mutations in four others identify the novel genes unc-129, seu-1, seu-2, and seu-3. Several of these mutations cause axon guidance defects similar to those found in unc-5 mutants. We propose that some or all of these genes may function in a developmentally important unc-5 signaling pathway.
Assuntos
Axônios/fisiologia , Proteínas de Caenorhabditis elegans , Caenorhabditis elegans/genética , Genes de Helmintos , Proteínas de Helminto/fisiologia , Proteínas de Membrana/fisiologia , Receptores de Superfície Celular , Receptores de Fatores de Crescimento/fisiologia , Animais , Anquirinas/fisiologia , Caenorhabditis elegans/fisiologia , Citoesqueleto/fisiologia , MutaçãoRESUMO
From egg through adult, C. elegans has six life stages including an option for dauer formation and diapause at larval stage L3 in adverse environments. Somatic cells throughout the organism make consistent choices and advance in unison, suggesting a mechanism of coordinate regulation at these stage transitions. Earlier studies showed that daf-12, which encodes a nuclear receptor (W. Yeh, 1991, Doctoral Thesis. University of Missouri-Columbia), regulates dauer formation; epistasis experiments placed daf-12 near the end of the dauer signaling pathway. Here we describe novel daf-12 alleles that reveal a general role in advancing L3 stage programs. In these mutants, somatic cells repeat L2-specific cellular programs of division and migration at the L3 stage; epistasis experiments place daf-12 between lin-14 and lin-28 within the heterochronic pathway. We propose daf-12 and other heterochronic genes provide cellular memories of chronological stage for selecting stage-appropriate developmental programs. Endocrine factors could coordinate these stage transitions and specify developmental alternatives.
Assuntos
Envelhecimento/fisiologia , Caenorhabditis elegans/crescimento & desenvolvimento , Regulação da Expressão Gênica no Desenvolvimento/genética , Genes de Helmintos/genética , Larva/crescimento & desenvolvimento , Receptores Citoplasmáticos e Nucleares/genética , Alelos , Animais , Movimento Celular/fisiologia , Mapeamento Cromossômico , Gônadas/crescimento & desenvolvimento , Larva/citologia , Larva/genética , Microscopia de Contraste de Fase , Muda/fisiologia , Mutagênese/genética , Fenótipo , Transdução de Sinais/fisiologia , Temperatura , Cromossomo X/genéticaRESUMO
Eight pairs of chemosensory neurons in Caenorhabditis elegans take up fluorescein dyes entering through the chemosensory organs. These are amphid neurons ADF, ASH, ASI, ASJ, ASK, and ADL and phasmid neurons PHA and PHB. When filled with dye, the processes and cell bodies of these neurons can be examined in live animals by fluorescence microscopy. Using this technique, we have identified five genes, unc-33, unc-44, unc-51, unc-76, and unc-106, that affect the growth of the amphid and phasmid axons. These genes were found to affect the axons of the mechanosensory PDE neurons as well. The unc-33 mutation specifically affects neuronal microtubules. Sensory dendrites in this mutant have a superabundance of microtubules. Moreover, many of these microtubules are abnormal in diameter, and some form hooks or multiple tubules.
Assuntos
Axônios/fisiologia , Caenorhabditis/ultraestrutura , Fluoresceínas/metabolismo , Neurônios Aferentes/fisiologia , Animais , Axônios/ultraestrutura , Caenorhabditis/genética , Fluoresceína-5-Isotiocianato , Genes , Locomoção , Microscopia Eletrônica , Microscopia de Fluorescência , Microtúbulos/ultraestrutura , Mutação , Neurônios Aferentes/ultraestrutura , Fenótipo , Tiocianatos/metabolismoRESUMO
Eight classes of chemosensory neurons in C. elegans fill with fluorescein when living animals are placed in a dye solution. Fluorescein enters the neurons through their exposed sensory cilia. Mutations in 14 genes prevent dye uptake and disrupt chemosensory behaviors. Each of these genes affects the ultrastructure of the chemosensory cilia or their accessory cells. In each case, the cilia are shorter or less exposed than normal, suggesting that dye contact is the principal factor under selection. Ten genes affect many or all of the sensory cilia in the head. The daf-19 (m86) mutation eliminates all cilia, leaving only occasional centrioles in the dendrites. The cilia in che-13 (e1805), osm-1 (p808), osm-5 (p813), and osm-6 (p811) mutants have normal transition zones and severely shortened axonemes. Doublet-microtubules, attached to the membrane by Y links, assemble ectopically proximal to the cilia in these mutants. The amphid cilia in che-11 (e1810) are irregular in diameter and contain dark ground material in the middle of the axonemes. Certain mechanocilia are also affected. The amphid cilia in che-10 (e1809) apparently degenerate, leaving dendrites with bulb-shaped endings filled with dark ground material. The mechanocilia lack striated rootlets. Cilia defects have also been found in che-2, che-3, and daf-10 mutants. The osm-3 (p802) mutation specifically eliminates the distal segment of the amphid cilia. Mutations in three genes affect sensillar support cells. The che-12 (e1812) mutation eliminates matrix material normally secreted by the amphid sheath cell. The che-14 (e1960) mutation disrupts the joining of the amphid sheath and socket cells to form the receptor channel. A similar defect has been observed in daf-6 mutants. Four additional genes affect specific classes of ciliated sensory neurons. The mec-1 and mec-8 (e398) mutations disrupt the fasciculation of the amphid cilia. The cat-6 (e1861) mutation disrupts the tubular bodies of the CEP mechanocilia. A cryophilic thermotaxis mutant, ttx-1 (p767), lacks fingers on the AFD dendrite, suggesting this neuron is thermosensory.