Characterization of color variation in bamboo sheath of Chimonobambusa hejiangensis by UPLC-ESI-MS/MS and RNA sequencing.

BACKGROUND: Chimonobambusa hejiangensis (C.hejiangensis) is a high-quality bamboo species native to China, known for its shoots that are a popular nutritional food. Three C.hejiangensis cultivars exhibit unique color variation in their shoot sheaths, however, the molecular mechanism behind this color change remains unclear. METHODS: We investigated flavonoid accumulation in the three bamboo cultivar sheaths using metabolomics and transcriptomics. RESULTS: UPLC-MS/MS identified 969 metabolites, with 187, 103, and 132 having differential accumulation in the yellow-sheath (YShe) vs. spot-sheath (SShe)/black-sheath (BShe) and SShe vs. BShe comparison groups. Flavonoids were the major metabolites that determined bamboo sheath color through differential accumulation of metabolites (DAMs) analysis. Additionally, there were 33 significantly differentially expressed flavonoid structural genes involved in the anthocyanin synthesis pathway based on transcriptome data. We conducted a KEGG analysis on DEGs and DAMs, revealing significant enrichment of phenylpropanoid and flavonoid biosynthetic pathways. Using gene co-expression network analysis, we identified nine structural genes and 29 transcription factors strongly linked to anthocyanin biosynthesis. CONCLUSION: We identified a comprehensive regulatory network for flavonoid biosynthesis which should improve our comprehension of the molecular mechanisms responsible for color variation and flavonoid biosynthesis in bamboo sheaths.

Daratumumab for Refractory IgD Multiple Myeloma with Lung Cancer and Persistent Thrombocytopenia: a Case Report.

BACKGROUND: Immunoglobulin D multiple myeloma (IgD-MM) is a rare but aggressive disease. The safety and effectiveness of anti-CD38 monoclonal antibody (daratumumab) have not been known in either IgD-MM or MM complicated with secondary neoplasm. METHODS: A fragile IgD-MM patient had an aggressively relapsed disease concurrent with lung cancer and severe thrombocytopenia, which led to a dilemma for management. After a failure of ixazomib-based chemotherapy, a salvage therapy with daratumumab unexpectedly induced complete remission and platelet recovery, and the patient successfully proceeded to lung cancer surgery. CONCLUSIONS: Our case indicates daratumumab is both safe and effective for refractory IgD-MM with severe complications.

A novel fluorescent probe based on a triphenylamine derivative for the detection of HSO3- with high sensitivity and selectivity.

A novel highly active fluorescence chemical sensor (TBQN) for HSO3- was synthesized by the Knoevenagel reaction based on triphenylamine-benzothiazole as a new fluorophore. The probe possessed good selectivity toward HSO3- and anti-interference ability with common ions. The fluorescence and UV-vis spectra of the TBQN probe were significantly changed after the addition of HSO3-. At the same time, the probe solution released obvious green fluorescence. Moreover, the limit of detection for HSO3- was calculated to be 3.19 × 10-8 M. The TBQN probe displayed a rapid response to HSO3- and it took about 3 min to complete the recognition. The detection mechanism is the nucleophilic addition reaction between HSO3- and -C[double bond, length as m-dash]C- in the probe molecule. The π-conjugation and ICT (intramolecular charge transfer) transition in the TBQN molecule were destroyed by this addition, which resulted in the change of the fluorescence before and after the addition of HSO3-. Then, the mechanism was verified by theoretical calculations, 1H NMR measurements and mass spectroscopy. In addition, the probe showed low cytotoxicity and could be used for biological imaging in RAW264.7 cells.