Selected freshwater fish species for assessing the water quality of the lower catchment of the Kelani River, Sri Lanka.

Kelani River is the most polluted river in Sri Lanka and the lower catchment is more polluted than the upper catchment. In the present study, freshwater fish species of the lower catchment of the river were investigated for the use of assessing the water quality. Cast net sampling and identification recorded 34 freshwater fish species from the lower catchment, the majority represented by family Cyprinidae. Fish species richness, diversity indices, distribution, abundance and the regression analysis of fish species with water quality parameters revealed high sensitivity and tolerance of three fish species with certain water quality parameters. Dawkinsia singhala was tolerant to the fluctuations of the chemical parameters of the water, while Rasbora daniconius and Pethia reval were tolerant to the physical parameters. Positive correlations were evident between the ammonium and phosphate concentrations of the water and distribution and abundance of D. singhala, while R. daniconius and P. reval showed positive correlations with turbidity of water and pH value respectively. Furthermore, the study reveals that D. singhala is more suitable for predicting the water quality of urban and peri-urban locations of the river, while P. reval and R. daniconius are more suitable for assessing the water quality of rural locations. Thus, the present study reveals a strong possibility of using D. singhala, R. daniconius and P. reval, as biological indicators for assessing the variation of water quality of the lower catchment of the Kelani River. However, despite the fact that such a study has been conducted for the first time in Sri Lanka, it is restrained by certain limitations, and seasonal variations of water quality parameters with fish parameters, adaptations inherent to fish species and food availability in different locations combined with long-term monitoring of fish assemblages have not been considered. Future studies investigating these aspects will further enhance the value of the study.

Lethal and sub-lethal effects on the Asian common toad Duttaphrynus melanostictus from exposure to hexavalent chromium.

Chromium discharged in industrial effluents frequently occurs as an environmental pollutant, but the lethal and sub-lethal effects the heavy metal might cause in animals exposed to it have been insufficiently investigated. Selecting the amphibian Duttaphrynus melanostictus, we carried out laboratory tests to investigate the effects of short and long term exposure to hexavalent chromium (Cr(VI)) in both tadpoles and adult toads. The concentrations used were 0.002, 0.02, 0.2, 1.0 and 2.0mg/L, the first three corresponding to field levels. In vitro exposures were also carried out using toad erythrocytes and Cr(VI) concentrations of 0.0015, 0.003, 0.015, 0.03, 0.15mg/L. Mortality, growth retardation, developmental delays and structural aberrations were noted in the metal-treated tadpoles, with increasing incidence corresponding to increase in Cr(VI) level and duration of exposure. Many of the sub-lethal effects were evident with long term exposure to environmentally relevant levels of the toxicant. Changes in selected blood parameters and erythrocyte morphometry were also detected in Cr(VI) exposed toads, indicating anaemic and leucopenic conditions. In the genotoxicity study, DNA damage indicated by comet assay and increased micronuclei frequency, occurred at the low Cr(VI) concentrations tested. The multiple deleterious effects of exposure to chromium signal the need for monitoring and controlling the discharge of chromium to the environment. The dose-dependency and genotoxic effects observed in this widely distributed Asian toad indicates its suitability for monitoring heavy metal pollution in aquatic systems.