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1.
Rev Esp Enferm Dig ; 116(4): 230-231, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-37170551

RESUMO

Intestinal ganglioneuromatosis (GN) is a benign disease of the autonomic nervous system characterized by hyperplasia of intramural plexuses of the gastrointestinal tract and enteric nerve fibers. Next, we expose an intestinal ganglioneuromatosis case that was difficult to diagnose despite an exhaustive evaluation, for further understanding of the disease.


Assuntos
Ganglioneuroma , Intestinos , Humanos , Ganglioneuroma/diagnóstico por imagem
2.
Can Vet J ; 61(11): 1155-1158, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-33149351

RESUMO

Clinical signs, imaging findings and long-term follow-up of 3 West Highland white terrier dogs with constrictive myelopathy secondary to caudal articular vertebral process dysplasia are described. Clinical signs were consistent with an acute or chronic T3-L3 myelopathy in all dogs. Diagnostic imaging revealed hypoplasia or aplasia of the caudal articular vertebral processes, extradural compressive myelographic pattern (hourglass-like pattern) with a reduced diameter of the spinal cord, and focal thickening of extradural soft tissues. Medical treatment initially improved the clinical signs in 2 dogs; however, mild proprioceptive deficits remained in all cases. Key clinical message: Constrictive myelopathy secondary to caudal articular vertebral process dysplasia in West Highland white terrier dogs should be considered as a differential diagnosis of an acute or chronic T3-L3 myelopathy in this breed.


Myélopathie constrictive secondaire à une dysplasie du processus articulaire caudal vertébral chez des chiens terriers West Highland white. Les signes cliniques, les trouvailles en imagerie et le suivi à longterme de trois chiens West Highland white avec myélopathie constrictive secondaire à une dysplasie du processus articulaire caudal vertébral sont décrits. Les signes cliniques étaient compatibles avec une myélopathie aiguë ou chronique au niveau T3­L3 chez tous les chiens. L'imagerie diagnostique a révélé une hypoplasie ou une aplasie des processus articulaires caudaux vertébraux, un patron myélographique de compression extra-dural (patron en sablier) avec un diamètre réduit de la moëlle épinière, et épaississement focal des tissus mous extra-duraux. Un traitement médical permis une amélioration des signes cliniques chez deux des chiens; toutefois, des déficits légers de proprioception ont persisté dans tous les cas.Message clinique clé:Une myélopathie constrictive secondaire à une dysplasie du processus articulaire caudal vertébral chez des chiens terriers West Highland white devrait être considérée comme un diagnostic différentiel d'une myélopathie aiguë ou chronique de T3­L3 chez cette race.(Traduit par Dr Serge Messier).


Assuntos
Doenças do Cão , Doenças da Medula Espinal , Animais , Diagnóstico Diferencial , Doenças do Cão/diagnóstico , Doenças do Cão/etiologia , Cães , Doenças da Medula Espinal/etiologia , Doenças da Medula Espinal/veterinária
3.
Hum Genet ; 138(5): 509-513, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30847549

RESUMO

Startle disease, or hyperekplexia, is a glycinergic disorder characterized by hypertonia and apnea that is triggered by noise and/or touch. Mutations in five genes have been associated with startle disease in humans, dogs, cattle, and mice. We identified a novel recessive startle disease in a family of Spanish greyhounds. Whole genome resequencing of an affected dog revealed a homozygous two base pair deletion in the ninth exon of SLC6A5, encoding the presynaptic glycine transporter. The deletion is predicted to cause a frameshift, p.S460FfsX47, leading to a premature stop codon that truncates over a third of the protein. Family members were genotyped for the deletion, and findings were consistent with an autosomal recessive inheritance pattern. The pathogenic variant was absent from 34 unrelated greyhounds, 659 domestic dogs of pure and mixed breeds, and 54 wild canids, suggesting it occurred recently and may be private to the family. The findings of this study can be used to inform future breeding decisions and prevent dissemination of the deleterious allele in greyhounds.


Assuntos
Doenças do Cão/genética , Mutação da Fase de Leitura/genética , Proteínas da Membrana Plasmática de Transporte de Glicina/genética , Rigidez Muscular Espasmódica/genética , Rigidez Muscular Espasmódica/veterinária , Animais , Códon sem Sentido/genética , Modelos Animais de Doenças , Cães , Deleção de Sequência/genética , Sequenciamento Completo do Genoma
4.
Vet Radiol Ultrasound ; 60(1): E1-E5, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28449234

RESUMO

An 8-year-old female Boxer was examined for acute onset of seizures. On magnetic resonance imaging (MRI), an intra-axial mass with imaging features consistent with glioma was observed in the right cerebral hemisphere. A defect in the temporal bone adjacent to the mass was observed. Postmortem computed tomography (CT) confirmed temporal bone osteolysis and necropsy demonstrated a glioblastoma with associated calvarial erosion. Although occasionally described in human medicine, to our knowledge, this is the first description of a brain glioma causing calvarial erosion in a dog. Glioma should be included as a differential diagnosis for intracranial lesions that could cause bony changes in the skull.


Assuntos
Neoplasias Encefálicas/veterinária , Doenças do Cão/diagnóstico por imagem , Glioma/veterinária , Osteólise/veterinária , Crânio/patologia , Animais , Neoplasias Encefálicas/diagnóstico por imagem , Neoplasias Encefálicas/patologia , Diagnóstico Diferencial , Doenças do Cão/patologia , Cães , Feminino , Glioma/diagnóstico por imagem , Glioma/patologia , Imageamento por Ressonância Magnética , Osteólise/diagnóstico por imagem , Osteólise/patologia , Crânio/diagnóstico por imagem , Osso Temporal/patologia , Tomografia Computadorizada por Raios X
7.
Vet Radiol Ultrasound ; 55(4): 424-7, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-23815770

RESUMO

An 8-year-old, male Boxer was examined for an acute onset of ambulatory paraparesis. Neurologic examination was consistent with a T3-L3 myelopathy. Myelography revealed an extradural spinal cord compression in the region of the T10-T13 vertebrae. On magnetic resonance (MR) imaging, a well-defined epidural mass lesion was detected. The mass was mildly hyperintense on T1-weighted, hyperintense on T2-weighted and STIR images compared to normal spinal cord and enhanced strongly and homogenously. Postmortem examination confirmed a primary epidural hemangiosarcoma. Findings indicated that the MRI characteristics of spinal epidural hemangiosarcoma may mimic other lesions including meningioma and epidural hemorrhages/hematomas of non-neoplastic etiology.


Assuntos
Doenças do Cão/diagnóstico , Hematoma Epidural Espinal/veterinária , Paraplegia/veterinária , Compressão da Medula Espinal/veterinária , Animais , Diagnóstico Diferencial , Doenças do Cão/etiologia , Cães , Eutanásia , Hematoma Epidural Espinal/diagnóstico , Hematoma Epidural Espinal/etiologia , Imageamento por Ressonância Magnética/veterinária , Masculino , Mielografia/veterinária , Paraplegia/diagnóstico , Paraplegia/etiologia , Paraplegia/cirurgia , Compressão da Medula Espinal/diagnóstico , Compressão da Medula Espinal/etiologia , Vértebras Torácicas/patologia
8.
Vet Radiol Ultrasound ; 55(3): 300-4, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-23738896

RESUMO

A 3-year-old, intact female Golden Retriever was presented with acute tetraplegia. Neurologic examination was consistent with a C1-C5 myelopathy. On magnetic resonance (MR) imaging a well-defined, extradural mass was detected within the spinal canal at the level of C1-C2. The mass was isointense to normal spinal cord gray matter on T1-weighted (T1W) images, hyperintense on T2-weighted (T2W), and gradient-echo (GE) images, and enhanced homogeneously after intravenous contrast administration. MR imaging features were mainly consistent with a meningioma. Surgical treatment was refused by the owners, and the dog was euthanized. Postmortem examination demonstrated that the intraspinal mass was a schwannoma.


Assuntos
Doenças do Cão/diagnóstico , Meningioma/veterinária , Neurilemoma/veterinária , Animais , Diagnóstico Diferencial , Doenças do Cão/patologia , Cães , Feminino , Imageamento por Ressonância Magnética/veterinária , Meningioma/diagnóstico , Meningioma/patologia , Pescoço/patologia , Neurilemoma/diagnóstico , Neurilemoma/patologia , Quadriplegia , Doenças da Medula Espinal
9.
J Avian Med Surg ; 28(4): 309-15, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25843469

RESUMO

A 5-month-old African grey parrot (Psittacus erithacus) was examined after 3 weeks of weakness, ataxia, mental depression, and seizures. Results of a complete blood cell count and plasma biochemical analysis were unremarkable. Magnetic resonance imaging revealed a severe bilateral hydrocephalus. The bird failed to improve with supportive care, and the owner requested euthanasia. Necropsy findings were severe bilateral hydrocephalus with no evidence of cerebrospinal fluid obstruction. Histologic examination of the brain revealed microspongiosis, edema, gliosis, and neuronal chromatolysis of surrounding periventricular tissue. Aquaporins (AQP) and astrocytes were examined to elucidate the participation of these water channel proteins and glial cells in the pathophysiology and resolution of hydrocephalus. Results showed AQP4 and glial fibrillary acidic protein were overexpressed, especially near the ventricles, but expression of AQP1 was decreased. This is the first report, to our knowledge, of AQP immunolabeling in hydrocephalus in avain species.

10.
Can Vet J ; 54(10): 948-54, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24155414

RESUMO

Clinical, imaging, and histological features of 8 canine spinal meningiomas, including a cervical cystic meningioma with imaging and intraoperative features of an arachnoid cyst, are described. All meningiomas were histologically classified and graded following the international World Health Organization human classification for tumors. Six meningiomas were located in the cervical spinal cord. Myelography showed intradural/ extramedullary lesions in 3/4 cases. Magnetic resonance imaging revealed hyperintense intradural/extramedullary masses on pre-contrast T1-weighted and T2-weighted images with homogeneous contrast enhancement in 7/8 cases. One dog had a cerebrospinal fluid-filled subarachnoid cavity dorsal to the cervical spinal cord. A spinal arachnoid cyst was diagnosed on imaging, but the histopathological study of the resected tissue revealed a grade I meningothelial cystic meningioma. There were no differences in outcome associated with tumor grade and surgical treatment (6/8). Cystic meningioma should be considered in the differential diagnosis of intraspinal cystic lesions, and biopsy is necessary for definitive diagnosis.


Méningiome spinal chez les chiens : description de 8 cas incluant une nouvelle présentation radiologique et histopathologique. Les caractéristiques cliniques et histologiques et l'imagerie de 8 méningiomes spinaux canins, incluant un méningiome cystique cervical avec des caractéristiques intraopératoires et l'imagerie d'un kyste arachnoïde, sont décrites. Tous les méningiomes ont été classifiés histologiquement et ont été évalués en suivant la classification humaine des tumeurs de l'Organisation mondiale de la santé. Six méningiomes ont été repérés dans la colonne vertébrale cervicale. La myélographie a montré des lésions intradurales/extramédullaires dans 3 cas sur 4. L'imagerie par résonance magnétique a révélé des masses intradurales/extramédullaires hyperintenses sur les images précontraste pondérées T1 et pondérés T2 avec une augmentation de contraste homogène dans 7 cas sur 8. Un chien avait une cavité rachidienne remplie de liquide cérébrospinal dorsalement à la colonne vertébrale cervicale. Un kyste arachnoïde spinal a été diagnostiqué à l'imagerie, mais l'étude histopathologique du tissu réséqué a révélé un méningiome cystique méningothélial de grade I. Il n'y avait aucune différence au niveau des résultats associée au grade de la tumeur et au traitement chirurgical (6/8). Les méningiomes sclérokystiques devraient être considérés dans le diagnostic différentiel des lésions cystiques intraspinales et une biopsie est nécessaire pour un diagnostic définitif.(Traduit par Isabelle Vallières).


Assuntos
Doenças do Cão/diagnóstico , Neoplasias Meníngeas/veterinária , Meningioma/veterinária , Animais , Doenças do Cão/patologia , Doenças do Cão/cirurgia , Cães , Feminino , Masculino , Neoplasias Meníngeas/diagnóstico , Neoplasias Meníngeas/patologia , Neoplasias Meníngeas/cirurgia , Meningioma/diagnóstico , Meningioma/patologia , Meningioma/cirurgia , Estudos Retrospectivos
11.
Can Vet J ; 54(7): 679-82, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24155462

RESUMO

An 8-year-old cat was presented with severe neurological deficits secondary to a traumatic cervical spinal cord injury caused by an airgun pellet. This report describes, for the first time, the myelographic findings of a dural rupture in a cat and also describes a bilateral Horner's syndrome in a cat.


Déchirure durale et myélomalacie causées par le plomb d'un pistolet pneumatique chez un chat. Un chat âgé de 8 ans a été présenté avec des déficits neurologiques graves secondaires à une blessure traumatique de la moelle épinière cervicale causée par le plomb d'un pistolet pneumatique. Ce rapport décrit, pour la première fois, les résultats myélographiques d'une rupture durale chez un chat et aussi un syndrome de Horner chez un chat.(Traduit par Isabelle Vallières).


Assuntos
Doenças do Gato/patologia , Dura-Máter/lesões , Mielografia/veterinária , Traumatismos da Medula Espinal/veterinária , Ferimentos por Arma de Fogo/veterinária , Animais , Doenças do Gato/etiologia , Gatos , Traumatismos da Medula Espinal/patologia , Ferimentos por Arma de Fogo/patologia
12.
Vet Rec ; 190(12): e1529, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-35278224

RESUMO

BACKGROUND: As they have been seldomly described in the veterinary literature, the aims of this retrospective study were to describe the clinical presentation, MRI findings and long-term outcome after medical or surgical treatment of dogs presenting with foraminal and far lateral thoracolumbar intervertebral disc herniations. METHODS: Retrospective multicentre study of dogs diagnosed with foraminal and far lateral thoracolumbar intervertebral disc herniations between 2009 and 2020 in seven referral hospitals. RESULTS: Thirty-seven dogs were included. Dachshunds and mixed breeds were most affected. Median age at presentation and duration of clinical signs were 6 years and 14 days, respectively. Pain was the most frequent clinical finding (92%), particularly on spinal palpation and/or hip manipulation, followed by pelvic limb lameness (71%). Eighty-seven percent of herniations occurred at L5-L6 or L6-L7. A good to excellent outcome was seen in 95% of surgically and 90% of medically treated dogs. CONCLUSION: Although much less frequently recognised than herniation affecting the vertebral canal, foraminal and far lateral thoracolumbar intervertebral disc herniations should be considered in the differential diagnosis list of chondrodystrophic dogs presenting with pain and pelvic limb lameness. Most dogs had a good to excellent outcome following medical or surgical treatment.


Assuntos
Doenças do Cão , Deslocamento do Disco Intervertebral/veterinária , Disco Intervertebral , Animais , Doenças do Cão/diagnóstico por imagem , Doenças do Cão/etiologia , Doenças do Cão/cirurgia , Cães , Disco Intervertebral/diagnóstico por imagem , Disco Intervertebral/patologia , Deslocamento do Disco Intervertebral/complicações , Deslocamento do Disco Intervertebral/diagnóstico por imagem , Deslocamento do Disco Intervertebral/cirurgia , Coxeadura Animal/etiologia , Vértebras Lombares/diagnóstico por imagem , Imageamento por Ressonância Magnética/veterinária , Dor/etiologia , Dor/veterinária , Estudos Retrospectivos , Vértebras Torácicas/diagnóstico por imagem
13.
Top Companion Anim Med ; 43: 100509, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33503466

RESUMO

A 6-year-old, female spayed rabbit (Oryctolagus cuniculus) presented with right paradoxical vestibular signs. Magnetic resonance imaging was performed and findings were consistent with an ischemic infarct of the cerebellum. The patient improved gradually and was free of clinical signs at the time this article was written. To the authors' knowledge this is the first case report of a paradoxical vestibular syndrome in a rabbit secondary to a presumptive ischemic infarct. Strokes should be included in the differential diagnosis of central vestibular syndrome in rabbits.


Assuntos
Doenças Cerebelares , Coelhos , Animais , Doenças Cerebelares/diagnóstico , Doenças Cerebelares/veterinária , Diagnóstico Diferencial , Feminino , Infarto/veterinária , Imageamento por Ressonância Magnética
14.
Vet Clin Pathol ; 50(3): 359-368, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34510505

RESUMO

BACKGROUND: Microscopic cell counts and nucleated cell identification are the "gold standard" for cerebrospinal fluid (CSF) assessments and are labor intensive and subject to operator variability. The use of automated methods could be an alternative to the current manual technique. OBJECTIVES: We aimed to assess the utility of the Sysmex XN-V body fluid (BF) module analyzer to count and differentiate nucleated cells in canine CSF and evaluate the accuracy and correlation between this and the manual method. METHODS: We prospectively analyzed 150 CSF samples from dogs using the Sysmex XN-V BF module and compared the results with those obtained using the manual counting method. We also evaluated the linearity, detection limits, and imprecision of the Sysmex XN-V BF module. RESULTS: The Sysmex XN-V BF module analyzer performance had a sensitivity of 92.59% and specificity of 94.30%. The lower limit of quantification for the total nucleated cell count (TNCC) was 0 cells/µL. A Pearson´s correlation coefficient of 0.945 was found between both methods for TNCC, with 0.997 and 0.940 for samples with TNCC >10 cells/µL and TNCC >5 cells/µL, respectively. The correlation coefficient for the mononuclear and polymorphonuclear differential cell count was -0.031 and -0.019, respectively, and it was 0.576 for the RBC count. CONCLUSIONS: The Sysmex XN-V BF module provides reliable TNCCs for canine CSF, even for samples with low cell numbers, but manual cytologic evaluation is still needed for differential cell counts.


Assuntos
Líquidos Corporais , Animais , Contagem de Células/veterinária , Líquido Cefalorraquidiano , Cães , Contagem de Eritrócitos/veterinária , Contagem de Leucócitos/veterinária , Neutrófilos , Reprodutibilidade dos Testes
15.
J Vet Intern Med ; 35(4): 1902-1917, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34117807

RESUMO

BACKGROUND: Gliomas in dogs remain poorly understood. OBJECTIVES: To characterize the clinicopathologic findings, diagnostic imaging features and survival of a large sample of dogs with glioma using the Comparative Brain Tumor Consortium diagnostic classification. ANIMALS: Ninety-one dogs with histopathological diagnosis of glioma. METHODS: Multicentric retrospective case series. Signalment, clinicopathologic findings, diagnostic imaging characteristics, treatment, and outcome were used. Tumors were reclassified according to the new canine glioma diagnostic scheme. RESULTS: No associations were found between clinicopathologic findings or survival and tumor type or grade. However, definitive treatments provided significantly (P = .03) improved median survival time (84 days; 95% confidence interval [CI], 45-190) compared to palliative treatment (26 days; 95% CI, 11-54). On magnetic resonance imaging (MRI), oligodendrogliomas were associated with smooth margins and T1-weighted hypointensity compared to astrocytomas (odds ratio [OR], 42.5; 95% CI, 2.42-744.97; P = .04; OR, 45.5; 95% CI, 5.78-333.33; P < .001, respectively) and undefined gliomas (OR, 84; 95% CI, 3.43-999.99; P = .02; OR, 32.3; 95% CI, 2.51-500.00; P = .008, respectively) and were more commonly in contact with the ventricles than astrocytomas (OR, 7.47; 95% CI, 1.03-53.95; P = .049). Tumor spread to neighboring brain structures was associated with high-grade glioma (OR, 6.02; 95% CI, 1.06-34.48; P = .04). CONCLUSIONS AND CLINICAL IMPORTANCE: Dogs with gliomas have poor outcomes, but risk factors identified in survival analysis inform prognosis and the newly identified MRI characteristics could refine diagnosis of tumor type and grade.


Assuntos
Neoplasias Encefálicas , Doenças do Cão , Glioma , Oligodendroglioma , Animais , Neoplasias Encefálicas/diagnóstico por imagem , Neoplasias Encefálicas/veterinária , Doenças do Cão/diagnóstico por imagem , Cães , Glioma/diagnóstico por imagem , Glioma/veterinária , Imageamento por Ressonância Magnética/veterinária , Oligodendroglioma/veterinária , Estudos Retrospectivos , Análise de Sobrevida
16.
Vet Rec ; 187(9): 356, 2020 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-32532842

RESUMO

BACKGROUND: Despite appropriate antiseizure drug (ASD) treatment, around two-thirds of dogs with idiopathic epilepsy (IE) have seizures long-term and 20-30per cent of affected dogs remain poorly controlled. METHODS: The current study aim is to test in a field trial the efficacy and tolerability of a commercially available diet enriched with 6.5per cent medium chain triglyceride (MCT) oil in dogs (n=21) with at least a tier 1 idiopathic epilepsy diagnosis, without cluster seizures, in 10 veterinary practices across Europe. Each dog's quality of life (QoL), ataxia, sedation and frequency and severity of seizures were recorded by owners throughout the study. RESULTS: The mean seizure frequency per month, averaged over the entire 84-day study, significantly (P=0.04) decreased 32per cent compared with the baseline monthly seizure frequency recorded during the month immediately before feeding the diet. Similarly, the seizure days rate (days/month) also declined (P<0.001) by 42per cent. QoL was reported as very good to excellent (>8.5/10) in 20 of the 21 dogs before starting the diet and this remained unchanged during the trial. CONCLUSIONS: This study demonstrates the use of a diet enriched with MCTs as an adjunct to ASD treatment may have some antiseizure properties for dogs diagnosed with IE, as demonstrated in previous studies.


Assuntos
Anticonvulsivantes/farmacologia , Convulsões/veterinária , Triglicerídeos/farmacologia , Ração Animal/análise , Animais , Anticonvulsivantes/administração & dosagem , Dieta/veterinária , Suplementos Nutricionais/análise , Cães , Relação Dose-Resposta a Droga , Europa (Continente) , Feminino , Masculino , Distribuição Aleatória , Convulsões/dietoterapia , Triglicerídeos/administração & dosagem , Triglicerídeos/metabolismo
17.
Vet Rec ; 181(22): 594-599, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29051312

RESUMO

MRI is considered gold standard for the diagnosis of presumptive acute hydrated non-compressive nucleus pulposus extrusions (AHNCNPE). This retrospective study describes the myelographic findings in dogs with AHNCNPE diagnosed by low-field MRI and their association with neurological grade, need of surgical decompression and outcome. Forty-two myelographies (21 dogs with presumptive AHNCNPE, 21 dogs with Hansen type I disc disease herniation) were blindly evaluated. Site of herniation, compression pattern, ratio of length of the lesion to length of the second lumbar vertebra (LL:L2) and degree of spinal cord compression (SCC) were measured on the myelographies of dogs with presumptive AHNCNPE and were compared with the corresponding MRI features. Percentage of extruded volume of nucleus pulposus (VNP) was calculated on MR images. Myelographic interobserver agreement for presumptive diagnosis of AHNCNPE was almost perfect (κ=0.8). Accuracy of myelography to detect site of herniation was 80.9 per cent and to identify extradural compression was 57.1 per cent. Mean SCC was 5.8±2.6 per cent for myelography and 6.6±3 per cent for MRI. Mean LL:L2 ratio was 1.7±0.9 for myelography and 1.2±0.8 for MRI. Mean percentage of extruded VNP was 40±14 per cent, and it was positively associated with neurological grade.


Assuntos
Doenças do Cão/diagnóstico por imagem , Imageamento por Ressonância Magnética/veterinária , Núcleo Pulposo/diagnóstico por imagem , Compressão da Medula Espinal/veterinária , Doença Aguda , Animais , Cães , Feminino , Vértebras Lombares , Imageamento por Ressonância Magnética/métodos , Masculino , Estudos Retrospectivos , Compressão da Medula Espinal/diagnóstico por imagem
20.
Vet J ; 209: 125-32, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26831167

RESUMO

Gliomas are the most common primary brain tumours in humans and are associated with a poor prognosis. An accurate animal model of human glioma tumorigenesis is needed to test new treatment strategies. Dogs represent a promising model because they develop spontaneous diffusely-infiltrating gliomas. This study investigated whether spontaneous canine gliomas contain cancer stem cells previously identified in all grades of human gliomas. Twenty spontaneous cases of canine gliomas were graded according to the human WHO classification. The expression of different markers of lineage differentiation was evaluated with immunohistochemistry as follows: nestin and CD133 for neural stem cells, doublecortin for neuronal progenitor cells, Olig2 for glial progenitor cells, glial fibrillary acidic protein, vimentin and S-100 for mature glial cells, and NeuN and ßIII-tubulin for mature neurons. Gliomas were characterised as follows: five grade II (oligodendrogliomas); nine grade III (seven anaplastic oligodendrogliomas, one anaplastic astrocytoma, one anaplastic oligoastrocytoma); six grade IV (glioblastomas). Immunohistochemical evaluation revealed that (1) nestin and CD133 were expressed in all grades of gliomas with a higher proportion of positive cells in high-grade gliomas; (2) the expression of S-100 protein and Olig2 did not differ substantially between astrocytic and oligodendroglial tumours, and (3) all gliomas were negative for mature neuron markers. The results demonstrated the presence of undifferentiated neural progenitors in all grades of spontaneous canine gliomas, confirming the relevance of this animal model for further studies on cancer stem cells.


Assuntos
Neoplasias Encefálicas/veterinária , Doenças do Cão/etiologia , Glioma/veterinária , Células-Tronco Neoplásicas/fisiologia , Animais , Neoplasias Encefálicas/fisiopatologia , Linhagem da Célula , Doenças do Cão/fisiopatologia , Cães , Feminino , Glioma/etiologia , Glioma/fisiopatologia , Imuno-Histoquímica/veterinária , Masculino , Gradação de Tumores/veterinária , Células-Tronco Neoplásicas/classificação
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