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1.
Br J Dermatol ; 178(3): 749-760, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-28815554

RESUMO

BACKGROUND: Striae gravidarum (SG), or stretch marks of pregnancy, begin as erythematous streaks and mature into hypopigmented atrophic bands. OBJECTIVES: In order to investigate molecular alterations that may promote atrophy of SG, we investigated dermal type I collagen fibrils, which provide human skin with support. METHODS: We obtained skin samples of recently developed, erythematous abdominal SG from pregnant women. To examine the organization of collagen fibrils, second-harmonic generation imaging was performed using multiphoton microscopy. Immunostaining was used to determine protein expression and localization of type I procollagen, the precursor of type I collagen fibrils. Real-time polymerase chain reaction was used to determine gene expression levels. RESULTS: In control (hip) and stretched normal-appearing perilesional abdominal skin, dermal collagen fibrils were organized as tightly packed, interwoven bundles. In SG, collagen bundles appeared markedly separated, especially in the mid-to-deep dermis. In the spaces separating these bundles, loosely packed wavy collagen fibrils lacking organization as bundles were present. These disorganized fibrils persisted into the postpartum period and failed to form densely packed bundles. Numerous large fibroblasts displaying type I procollagen expression were in close proximity to the disorganized fibrils, suggesting that the fibrils are newly synthesized. Supporting this possibility, immunostaining and gene expression of type I procollagen were increased throughout the dermis of SG. CONCLUSIONS: Early SG display marked separation of collagen bundles and emergence of disorganized collagen fibrils that fail to form bundles. These alterations may reflect ineffective repair of collagen bundles disrupted by intense skin stretching. Persistent disruption of the collagenous extracellular matrix likely promotes formation and atrophy of SG.


Assuntos
Doenças do Colágeno/patologia , Complicações na Gravidez/patologia , Estrias de Distensão/patologia , Estudos de Casos e Controles , Doenças do Colágeno/metabolismo , Colágeno Tipo I/metabolismo , Feminino , Colágenos Fibrilares/fisiologia , Fibroblastos/metabolismo , Humanos , Gravidez , Complicações na Gravidez/metabolismo , Pró-Colágeno/biossíntese , Pele/irrigação sanguínea , Estrias de Distensão/metabolismo , Adulto Jovem
2.
Br J Dermatol ; 173(6): 1420-30, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26179468

RESUMO

BACKGROUND: Striae gravidarum (SG), or 'stretch marks' of pregnancy, begin as erythematous streaks, and mature over months to years to become permanent scar-like bands that may be hypopigmented, atrophic and lax. OBJECTIVES: To investigate early molecular alterations that may promote laxity of mature SG, we investigated the dermal elastic fibre network, which provides human skin with elastic properties. METHODS: We obtained skin samples of newly developed, erythematous abdominal SG in healthy pregnant women. The elastic fibre network was examined by Verhoeff elastic staining and immunofluorescence staining of skin sections. Gene expression was measured by real-time polymerase chain reaction. RESULTS: The normal elastic fibre network appeared markedly disrupted in SG, compared with perilesional abdominal skin or control (normal-appearing hip skin). This disruption was accompanied by the emergence of short, disorganized, thin, thread-like 'fibrils', which were observed prominently in the mid-to-deep dermis. These fibrils were rich in tropoelastin (the main component of normal elastic fibres), and persisted into the postpartum period without forming normal-appearing elastic fibres. The emergence of these fibrils was accompanied by increased gene expression of tropoelastin and fibrillin-1, but not other elastic fibre components, including fibrillin-2 and fibulin-1, -2 or -5. CONCLUSIONS: In early SG, the elastic fibre network appears markedly disrupted, and newly synthesized tropoelastin-rich fibrils emerge, likely as a result of uncoordinated synthesis of elastic fibre components. Because they are thin and disorganized, tropoelastin-rich fibrils likely do not function as normal elastic fibres do. These observations provide the foundations for elucidating pathogenic mechanisms by which laxity may develop in SG.


Assuntos
Tecido Elástico/patologia , Estrias de Distensão/patologia , Doenças do Colágeno/patologia , Tecido Elástico/metabolismo , Feminino , Humanos , Gravidez , Transtornos Puerperais/metabolismo , Transtornos Puerperais/patologia , Estrias de Distensão/metabolismo , Tropoelastina/metabolismo , Adulto Jovem
3.
Clin Case Rep ; 11(5): e7182, 2023 May.
Artigo em Inglês | MEDLINE | ID: mdl-37180317

RESUMO

Sarcomatoid sweat gland carcinomas are rare among cutaneous cancers, with less than 20 cases described. A 54-year-old woman with sarcomatoid sweat gland carcinoma of the right upper extremity suffered extensive recurrence at 15 months, unresponsive to chemotherapy. There is no standard treatment or chemotherapy regimens for metastatic sweat gland carcinoma.

4.
J Prev Med Hyg ; 60(1): E36-E42, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31041409

RESUMO

INTRODUCTION: Since Vietnam has signed WHO framework on tobacco control (FCTC) in 2003 and has issued tobacco control law in 2013, there has been little research concerning about what impacts smoke-free regulations have had on public compliance. The objective of this study was to assess public exposure to secondhand smoke and reaction toward smoke-free policy regulations in Vietnam and the associated factor. METHODS: Using the design of GATS (Global Adult Tobacco Survey), a nationally representative sample of 8,996 adults were approached for data collection. Logistic regression was used to examine the associated factor. RESULTS: The study revealed that the prevalence of respondents exposed to secondhand smoke was much higher in bars/café/tea shops (90.07%) and restaurants (81.81%) than in any other public places, universities (36.70%), government buildings (31.12%), public transport (20.04%), healthcare facilities (17.85%) and schools (15.84%). 13.23% of respondents saw smokers violate smoke-free regulations. Among those who saw them violate smoke-free regulations, just one-third cautioned them to stop smoking. Strikingly, a higher rate of cautioning smokers to stop smoking was observed among the older, married, and better educated respondents. Respondents who were married, better educated and in lower economic status were more likely to remind smokers to stop smoking. CONCLUSIONS: The study has called for strengthening two of the six MPOWER (Monitor, Protect, Offer, Warn, Enforce and Raise) components of the tobacco free initiative introduced by WHO, Monitoring tobacco use and prevention policies and Protecting people from tobacco smoke.


Assuntos
Crime/estatística & dados numéricos , Política Antifumo/legislação & jurisprudência , Poluição por Fumaça de Tabaco/estatística & dados numéricos , Adolescente , Adulto , Idoso , Feminino , Instalações de Saúde , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Restaurantes , Instituições Acadêmicas , Inquéritos e Questionários , Poluição por Fumaça de Tabaco/legislação & jurisprudência , Poluição por Fumaça de Tabaco/prevenção & controle , Meios de Transporte , Universidades , Vietnã , Adulto Jovem
5.
Eur J Clin Nutr ; 62(6): 810-6, 2008 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-17622264

RESUMO

OBJECTIVE: To describe the household intakes of retinol and carotenoids and social economic factors determining their intakes. SUBJECTS: Data on a total of 1001 households (771 in rural areas and 230 in urban areas) were used in the analyses. Interviewed person was household food preparer. RESULTS: Mean (s.d.) intake of carotenoids was 4178 (3154) microg/capita/day in rural and 4208 (3408) microg/capita/day in urban areas and intake of retinol was 101 (275) microg/capita/day in rural and 201 (470) microg/capita/day in urban areas. Multivariate analyses show that the subjects in households with four or more members consume about 700 microg carotenoids less compared to households with less than three members. Households with a higher expenditure (fourth quartile) consumed about 100 microg retinol/day more than those with a lower expenditure (first quartile). CONCLUSION: Carotenoids from plant food sources is the main source of vitamin A intake of the population and its main determinants are household expenditure and size of household. Food fortification and dietary diversification with special emphasis on promotion of consumption of animal foods should be key strategies for overcoming vitamin A deficiency in Vietnam.


Assuntos
Carotenoides/administração & dosagem , Dieta , População Rural/estatística & dados numéricos , População Urbana/estatística & dados numéricos , Deficiência de Vitamina A/prevenção & controle , Vitamina A/administração & dosagem , Análise de Variância , Análise por Conglomerados , Estudos Transversais , Características da Família , Feminino , Promoção da Saúde , Humanos , Masculino , Análise Multivariada , Fatores Socioeconômicos , Inquéritos e Questionários , Vietnã/epidemiologia , Deficiência de Vitamina A/dietoterapia , Deficiência de Vitamina A/epidemiologia
7.
Eur J Clin Nutr ; 58(12): 1594-603, 2004 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-15226755

RESUMO

OBJECTIVE: To assess the performance of low BMI, and define optimal BMI cut-off values in order to detect fever-associated adult morbidity. DESIGN: A cohort study of adults between 18 and 60 y in rural Vietnam, whose BMI and health status were assessed at baseline, and who were then monitored for illness events for 4 months. Nonparametric receiver operating characteristic (ROC) analysis was used to evaluate the performance of low BMI to detect the average number of restricted-days due to illness and to determine optimal cut-off values. SETTING: A rural commune in the Red River Delta, northern Vietnam. SUBJECTS: The study included 693 men and 739 women aged 18-60-y. RESULTS: At baseline, 21% of the study participants had a BMI<18.5 kg/m(2). As BMI progressively decreased, the percentage of participants experiencing morbidity with fever increased. The areas under the ROC curves for BMI were significantly greater than 0.5 for all levels of monthly average restricted-days of illness (MARDI) with fever, with best performance for >5 days/month. Excluding participants with acute or chronic disease at baseline improved the performance of BMI to detect MARDI with fever of >5 days (area under ROC curve 0.95; 95% CI 0.92, 0.99). With increasing levels of MARDI with fever, BMI cut-offs fell to 17.9 kg/m(2) when MARDI with fever was >5 days. CONCLUSIONS: The ROC analysis demonstrates that low BMI performs well as a risk indicator of MARDI with fever of >5 days with an optimal BMI cut-off value of 17.9 kg/m(2).


Assuntos
Índice de Massa Corporal , Peso Corporal/fisiologia , Distúrbios Nutricionais/mortalidade , Saúde da População Rural , Adolescente , Adulto , Antropometria , Área Sob a Curva , Estudos de Coortes , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Razão de Chances , Valor Preditivo dos Testes , Curva ROC , Padrões de Referência , Fatores de Risco , Sensibilidade e Especificidade , Vietnã
8.
J Bone Joint Surg Am ; 77(5): 661-73, 1995 May.
Artigo em Inglês | MEDLINE | ID: mdl-7744891

RESUMO

Twenty-one complex fractures of the tibial plateau in twenty patients were treated with closed reduction, interfragmental screw fixation of the articular fragments, and application of a unilateral half-pin external fixator. The average duration of external fixation was twelve weeks (range, three to twenty weeks). The fixator was left in situ until the fracture had united in all but two patients. All of the fractures healed. The complications with this technique were attributable primarily to the proximal half-pins of the external fixator. Seven patients needed antibiotics for an infection at a pin site, and two had septic arthritis that necessitated arthrotomy and débridement. The average duration of follow-up was thirty-eight months. The range of motion of nineteen of the twenty-one knees was at least a 115-degree arc. Laxity was evident in seven knees, but no patient complained of instability of the knee. Radiographs showed malalignment of more than 6 degrees in three knees compared with the normal, contralateral knee and evidence of post-traumatic osteoarthrosis in five knees. The Iowa knee score, determined for nineteen patients, averaged 87 points (range, 55 to 100 points). The SF-36 general health survey demonstrated that most patients had function close to that of age-matched controls. We concluded that external fixation with limited internal fixation is a satisfactory technique for the treatment of selected complex fractures of the tibial plateau.


Assuntos
Fixação de Fratura/métodos , Fraturas da Tíbia/cirurgia , Adulto , Fixadores Externos , Feminino , Seguimentos , Fixação Interna de Fraturas/métodos , Humanos , Masculino , Pessoa de Meia-Idade , Dor Pós-Operatória , Qualidade de Vida , Radiografia , Amplitude de Movimento Articular , Fraturas da Tíbia/diagnóstico por imagem
9.
Vet Parasitol ; 93(2): 141-7, 2000 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-11035232

RESUMO

Over a period of 13 months, faecal samples were collected monthly from approximately 45 cattle over 3 months of age. Additionally, 74 calves of 1-2 months were sampled to determine the presence of Toxocara vitulorum eggs. Individual egg counts and infective strongyle larvae from pooled faecal samples were examined. Post-mortem worm counts were carried out on six groups of tracer calves (n=12) that had been kept for 4 weeks on pasture in and around the village studied. The following helminths were identified: T. vitulorum, Cooperia punctata, C. pectinata, C. oncophora, Oesophagostomum radiatum, Trichostrongylus axei, T. colubriformis, Haemonchus spp., Fasciola spp. and Paramphistomum spp. In 8% of the samples collected from young calves, individual egg counts for T. vitulorum were found indicative for pathogenic worm burdens. Strongyle egg counts and worm counts indicated that transmission is low without a distinct seasonality. In animals of 3-9 months old, a strongyle egg count peak can be demonstrated which at a higher age steadily and significantly decreased. In faecal cultures Cooperia spp. were most prominent in all age groups throughout the year with the exception of the period September-November when Haemonchus spp. and Oesophagostomum spp. were most prevalent. Fasciola spp. eggs were found in 22% of the collected faecal samples and the egg counts were low indicating that the intensity of Fasciola spp. infection is mild. Based on the present data, regular anthelmintic treatments seem not to be justified, except for a single treatment at the age of 2 weeks against toxocariosis.


Assuntos
Doenças dos Bovinos/epidemiologia , Fasciolíase/veterinária , Infecções por Nematoides/veterinária , Animais , Bovinos , Fasciolíase/epidemiologia , Fezes/parasitologia , Infecções por Nematoides/epidemiologia , Contagem de Ovos de Parasitas/veterinária , Prevalência , Vietnã/epidemiologia
10.
Vet Parasitol ; 111(2-3): 115-23, 2003 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-12531288

RESUMO

Although Trypanosoma evansi is not considered as an important pathogen in pigs, it may interfere with other pathogens or vaccinations by its immunosuppressive nature. In order to determine whether T. evansi alters pig performance and induces immunosuppression in pigs, induction of immune responses by vaccination against classical swine fever (CSF) and by immunization with a control antigen, human serum albumin (HSA), was assessed in T. evansi-infected and non-infected animals. Although T. evansi infection did not have a significant influence on growth performance, feed conversion or PCV, antibody responses against both the test antigen HSA and the CSF vaccine were significantly reduced in T. evansi-infected animals as compared to uninfected animals. Moreover, the reduced response against the CSF vaccine appears to be accompanied by a less well-developed protection against CSF with higher fever responses and leukopenia. This immunosuppression might explain the accounts of poor protection of CSF-vaccinated pigs reported in T. evansi-endemic areas of Vietnam, and suggests that prior treatments with trypanocidal drugs to improve the efficacy of CSF vaccination, may be justified.


Assuntos
Peste Suína Clássica/imunologia , Doenças dos Suínos/imunologia , Trypanosoma/imunologia , Tripanossomíase/veterinária , Vacinação/veterinária , Animais , Anticorpos Antiprotozoários/sangue , Anticorpos Antivirais/sangue , Bioensaio , Biópsia/veterinária , Peste Suína Clássica/sangue , Peste Suína Clássica/parasitologia , Peste Suína Clássica/prevenção & controle , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Imunofluorescência , Hematócrito/veterinária , Contagem de Leucócitos/veterinária , Masculino , Camundongos , Parasitemia/parasitologia , Parasitemia/veterinária , Albumina Sérica/imunologia , Suínos , Doenças dos Suínos/parasitologia , Doenças dos Suínos/fisiopatologia , Doenças dos Suínos/virologia , Trypanosoma/crescimento & desenvolvimento , Tripanossomíase/imunologia , Tripanossomíase/fisiopatologia , Vacinas Virais/imunologia , Vacinas Virais/normas
11.
J Nutr Sci Vitaminol (Tokyo) ; 47(1): 64-8, 2001 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11349893

RESUMO

In order to have basic data for dietary preventive measures against some vascular diseases caused by disorder of lipid metabolism, food intake and lipid status were investigated in three Vietnamese populations with different per capita income levels. Totally, 299 people aged over 18 were involved in this study. Among them, 98 subjects were selected from one commune with low income, 100 and 101 subjects from two urban areas with medium and high income respectively. Food intake was assessed by 24-h recall method and lipid status was evaluated by measuring serum concentration of triglyceride, total cholesterol and its subfractions. The results showed that food consumption, dietary habits and lipid status varied with income levels. In general, diet was still unbalanced in all three groups, and low in fat and protein. In the rural, fat and protein intakes were lower than in the urban. The high prevalence of low serum cholesterol was observed in all three groups, the highest in the rural (45.3%) and the lowest in the urban (11.2%). On the contrary, the prevalence of high serum cholesterol was the lowest in the rural (2%) and the highest in the urban (16%). From these data, it is suggested that the majority of the Vietnamese might be vulnerable to some vascular diseases such as stroke if the situation of low fat intake and low serum cholesterol is not a problem of concern.


Assuntos
Doenças Cardiovasculares/sangue , Doenças Cardiovasculares/economia , Dieta/economia , Renda , Lipídeos/sangue , Adulto , Doenças Cardiovasculares/etiologia , Doenças Cardiovasculares/prevenção & controle , Colesterol/sangue , Gorduras na Dieta/administração & dosagem , Gorduras na Dieta/efeitos adversos , Proteínas Alimentares/administração & dosagem , Ingestão de Energia , Comportamento Alimentar , Feminino , Humanos , Masculino , Rememoração Mental , Pessoa de Meia-Idade , Estado Nutricional , População Rural , População Urbana , Vietnã
12.
Aquat Toxicol ; 118-119: 48-53, 2012 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-22516674

RESUMO

The tolerance and effects of nitrite on ion balance and haematology were investigated in the striped snakehead, Channa striata Bloch 1793, which is an air-breathing fish with reduced gills of importance for aquaculture in South East Asia. C. striata was nitrite tolerant with a 96 h LC50 of 4.7 mM. Effects of sub-lethal exposures to nitrite (0mM, 1.4mM, and 3.0mM) were determined during a 7-day exposure period. Plasma nitrite increased, but the internal concentration remained well below ambient levels. Extracellular nitrate rose by several mM, indicating that a large proportion of the nitrite taken up was converted to nitrate. Nitrite reacted with erythrocyte haemoglobin (Hb) causing methaemoglobin (metHb) to increase to 30% and nitrosylhaemoglobin (HbNO) to increase to 10% of total Hb. Both metHb and HbNO stabilised after 4 days, and functional Hb levels accordingly never fell below 60% of total Hb. Haematocrit and total Hb were unaffected by nitrite. Although the effects of nitrite exposure seemed minor in terms of plasma nitrite and metHb increases, ion balance was strongly affected. In the high exposure group, total osmolality decreased from 320 mOsm to 260 mOsm, and plasma sodium from 150 mM to 120 mM, while plasma chloride fell from 105 mM to 60mM and plasma bicarbonate rose from 12 mM in controls to 20mM in exposed fish. The extreme changes in ion balance in C. striata are different from the response reported in other fish, and further studies are needed to investigate the mechanism behind the observed changes in regulation.


Assuntos
Hemoglobinas/metabolismo , Metemoglobina/metabolismo , Nitritos/toxicidade , Perciformes/sangue , Poluentes Químicos da Água/toxicidade , Animais , Bicarbonatos/sangue , Cloretos/sangue , Hematócrito/veterinária , Nitritos/sangue , Nitritos/metabolismo , Concentração Osmolar , Distribuição Aleatória , Sódio/sangue , Poluentes Químicos da Água/metabolismo , Equilíbrio Hidroeletrolítico/efeitos dos fármacos
13.
Aquat Toxicol ; 104(1-2): 86-93, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21545779

RESUMO

In this study we investigated nitrite (NO2⁻) effects in striped catfish, a facultative air-breather. Fish were exposed to 0, 0.4, and 0.9 mM nitrite for 0, 1, 2, 4, and 7 days, and levels of functional haemoglobin, methaemoglobin (metHb) and nitrosyl haemoglobin (HbNO) were assessed using spectral deconvolution. Plasma concentrations of nitrite, nitrate, chloride, potassium, and sodium were also measured. Partitioning of oxygen consumption was determined to reveal whether elevated metHb (causing functional hypoxia) induced air-breathing. The effects of nitrite on maximum oxygen uptake (MO(2max)) and critical swimming speed (U(crit)) were also assessed. Striped catfish was highly tolerant to nitrite exposure, as reflected by a 96 h LC50 of 1.65 mM and a moderate nitrite uptake into the blood. Plasma levels of nitrite reached a maximum after 1 day of exposure, and then decreased, never exceeding ambient levels. MetHb, HbNO and nitrate (a nitrite detoxification product) also peaked after 1 day and then decreased. Only high levels of nitrite and metHb caused reductions in MO(2max) and U(crit). The response of striped catfish contrasts with that seen in most other fish species and discloses efficient mechanisms of combating nitrite threats. Furthermore, even though striped catfish is an efficient air-breather, this species has the ability to sustain aerobic scope and swimming performance without air-breathing, even when faced with nitrite-induced reductions in blood oxygen carrying capacity. Our study is the first to confirm that high levels of nitrite and metHb reduce MO(2max) and thereby aerobic scope, while more moderate elevations fail to do so. Further studies are needed to elucidate the mechanisms underlying the low nitrite accumulation in striped catfish.


Assuntos
Peixes-Gato/fisiologia , Hemoglobinas/metabolismo , Nitritos/toxicidade , Natação/fisiologia , Poluentes Químicos da Água/toxicidade , Animais , Peixes-Gato/metabolismo , Nitritos/sangue , Consumo de Oxigênio/efeitos dos fármacos , Respiração/efeitos dos fármacos , Poluentes Químicos da Água/sangue , Equilíbrio Hidroeletrolítico/efeitos dos fármacos
15.
Hosp Pharm ; 26(9): 823, 827, 1991 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-10114013
16.
J Bone Joint Surg Br ; 90(9): 1228-33, 2008 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-18757965

RESUMO

The aim of this retrospective multicentre study was to report the continued occurrence of compartment syndrome secondary to paediatric supracondylar humeral fractures in the period 1995 to 2005. The inclusion criteria were children with a closed, low-energy supracondylar fracture with no associated fractures or vascular compromise, who subsequently developed compartment syndrome. There were 11 patients (seven girls and four boys) identified from eight hospitals in three countries. Ten patients with severe elbow swelling documented at presentation had a mean delay before surgery of 22 hours (6 to 64). One patient without severe swelling documented at presentation suffered arterial entrapment following reduction, with a subsequent compartment syndrome requiring fasciotomy 25 hours after the index procedure. This series is noteworthy, as all patients had low-energy injuries and presented with an intact radial pulse. Significant swelling at presentation and delay in fracture reduction may be important warning signs for the development of a compartment syndrome in children with supracondylar fractures of the humerus.


Assuntos
Síndromes Compartimentais/etiologia , Fraturas Fechadas/complicações , Fraturas do Úmero/complicações , Complicações Pós-Operatórias/etiologia , Adolescente , Criança , Pré-Escolar , Feminino , Fraturas Fechadas/cirurgia , Humanos , Fraturas do Úmero/cirurgia , Masculino , Nova Zelândia , Estudos Retrospectivos , Medição de Risco , Fatores de Risco , Fatores de Tempo , Resultado do Tratamento , Reino Unido , Estados Unidos
17.
Trop Anim Health Prod ; 37(6): 457-67, 2005 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-16248217

RESUMO

This study is concerned with the evaluation of established diagnostic tests for diagnosis of Trypanosoma evansi in pigs. The immune trypanolysis test (TL), card agglutination test (CATT), latex agglutination test (LATEX), enzyme-linked immunosorbent assay (ELISA), microhaematocrit centrifugation technique (MHCT) and mouse inoculation (MI) tests were initially evaluated in experimentally infected fattening pigs. All infected pigs were confirmed parasitologically positive with both MHCT and MI. Results of the serological assays indicated that the TL could be a reference test for the presence of RoTat 1.2 antibodies in pigs. The results of the CATT and LATEX were inconsistent with the TL while the ELISA results correlated with the TL results. The four serological assays were subsequently used in two field surveys in Vietnam and Thailand. Results of the two agglutination assays (CATT and LATEX) were not consistent and did not correlate with TL results. The ELISA at percentage positivity of 22 appeared to have good ability to discriminate between seropositive and seronegative animals. Of the 437 samples collected at smallholder pig premises in northern Vietnam, no positive pigs were detected with the TL test. In Thailand, 77 samples were collected from five farrowing farms with a history of surra. Two parasitologically positive sows were found and on each farm seropositive sows were detected.


Assuntos
Testes Sorológicos/veterinária , Doenças dos Suínos/diagnóstico , Trypanosoma/isolamento & purificação , Tripanossomíase/veterinária , Testes de Aglutinação/métodos , Testes de Aglutinação/veterinária , Animais , Anticorpos Antiprotozoários/sangue , Ensaio de Imunoadsorção Enzimática/métodos , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Hematócrito/veterinária , Testes de Fixação do Látex/métodos , Testes de Fixação do Látex/veterinária , Masculino , Sensibilidade e Especificidade , Testes Sorológicos/métodos , Testes Sorológicos/normas , Suínos , Doenças dos Suínos/sangue , Doenças dos Suínos/parasitologia , Tailândia , Trypanosoma/imunologia , Tripanossomíase/sangue , Tripanossomíase/diagnóstico , Vietnã
18.
Curr Opin Pediatr ; 13(1): 42-6, 2001 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11176242

RESUMO

Lower extremity bowing is a normal physiologic process that commonly occurs in walking children under the age of 2 years. After this age, any significant amount of residual bowing is abnormal and may be due to infections, traumatic physeal injuries, genetic predisposition, metabolic factors, or a combination of these. Of the pathologic conditions, infantile Blount's disease is the most common and is predominantly found in obese children with an early onset of walking. Because of its frequency in occurrence and general tendency for spontaneous resolution, physiologic genu varum must be clearly distinguished from other pathologic processes that result in bowleggedness. Treatment of the latter cases may consist of early bracing or surgery.


Assuntos
Doenças do Desenvolvimento Ósseo/diagnóstico , Ossos da Perna , Acondroplasia/complicações , Adolescente , Doenças do Desenvolvimento Ósseo/etiologia , Doenças do Desenvolvimento Ósseo/terapia , Criança , Pré-Escolar , Feminino , Humanos , Ossos da Perna/anormalidades , Ossos da Perna/diagnóstico por imagem , Ossos da Perna/crescimento & desenvolvimento , Masculino , Osteocondrite/complicações , Osteocondrite/diagnóstico , Radiografia/métodos , Valores de Referência , Raquitismo/complicações , Raquitismo/diagnóstico
19.
Curr Opin Pediatr ; 12(1): 48-51, 2000 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-10676774

RESUMO

Transient synovitis of the hip is one of the most common causes of hip pain and limp in young children. Its cause is still largely unknown, but its natural history is that of a self-limiting disorder with no residual sequelae, although recurrences are possible. Because this benign condition is similar to more significant disorders, such as septic arthritis, the diagnosis should remain one of exclusion. Once transient synovitis is diagnosed, treatment consists of rest, anti-inflammatory agents, and a tincture of time.


Assuntos
Sinovite , Algoritmos , Anti-Inflamatórios/uso terapêutico , Artrite Infecciosa/diagnóstico , Criança , Pré-Escolar , Diagnóstico Diferencial , Marcha , Articulação do Quadril/diagnóstico por imagem , Humanos , Dor/tratamento farmacológico , Dor/etiologia , Recidiva , Fatores Sexuais , Sinovite/complicações , Sinovite/diagnóstico , Sinovite/terapia , Ultrassonografia
20.
Am J Hum Genet ; 31(5): 581-5, 1979 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-507052

RESUMO

Extraembryonic membranes and fetal tissues were obtained from 55 specimens of 5--11 weeks conceptual age. The glucose-6-phosphate dehydrogenase (G6PD) electrophoretic phenotype was determined and correlated with that of maternal blood. Fifteen specimens were heterozygous for G6PD A, and for nine of these the maternal allele could be determined. In none of these specimens did the isozyme pattern of the membraneous chorion or chorionic villi differ significantly from that of fetal tissue. We have obtained no evidence of non-random inactivation in extraembryonic membranes of human fetal specimens at this stage of development.


Assuntos
Membranas Extraembrionárias/enzimologia , Glucosefosfato Desidrogenase/genética , Cromossomos Sexuais , Cromossomo X , Herança Extracromossômica , Feminino , Variação Genética , Heterozigoto , Humanos , Isoenzimas/genética , Fenótipo , Gravidez
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