Application of Spectrophotometric Fingerprint in Cluster Analysis for Starch Origin Determination.

The botanical origin of starch is of importance in industrial applications and food processing because it may influence the properties of the final product. Current microscopic methods are time-consuming. Starch consists of an origin-dependent amylose/amylopectin ratio. Triiodide ions bind characteristically to the amylose and amylopectin depending on the botanical origin of the starch. The absorbance of the starch-triiodide complex was measured for: wheat, potato, corn, rye, barley, rice, tapioca and unknown origin starch; and within the different cultivars. Each starch sample had specific parameters: starch-triiodide complex peak wavelength maximum (λ max/nm), maximum absorbance change at λ max (ΔA) and λ max shift towards the unknown origin starch sample values. The visible absorption spectra (500-800 nm) for each starch sample were used as a unique fingerprint, and then elaborated by cluster analysis. The cluster analysis managed to distinguish data of two clusters, a cereal type cluster and a potato/tapioca/rice starch cluster. The cereal subclusters extensively distinguished wheat/barley/rye starches from corn starches. Data for cultivars were mostly in good agreement within the same subclaster. The proposed method that combines cluster analysis and visible absorbance data for starch-triiodide complex was able to distinguish starch of different botanical origins and cultivars within the same species. This method is simpler and more convenient than standard time-consuming methods.

Influence of Biological Therapeutics on Patient-Reported Quality-Of-Life Outcomes (Whoqol-Bref), Functional Scores and Disease Activity among Croatian Patients with Rheumatoid Arthritis: Our Experience.

BACKGROUND: Rheumatoid arthritis (RA) is a chronic and disabling disease with a great impact on the quality of life (QOL). The aim of this study was to assess QOL and health in RA patients treated with biological disease-modifying drugs (bDMARDs) as opposed to those treated with conventional synthetic DMARDs (csDMARDs). We analysed four domains of QOL: physical health (D1), mental health (D2), social relationships (D3) and one's surroundings (D4); as well as general quality of life (W1), general state of health (W2), and disease activity and physical disability. SUBJECTS AND METHODS: Seventy-seven RA patients (group A=29 on bDMARDs, group B=48 on csDMARDs) were enrolled in the study. QOL was evaluated using WHO questionnaire (WHOQOL-BREF), disease activity using Disease Activity Score 28C-reactive protein (DAS28CRP) and functional status using Health Assessment Questionnaire (HAQ). RESULTS: There was no statistically significant difference of mean values in the four domains of QOL, nor in the general QOL, between groups A and B. There was also no statistically significant difference regarding RA activity (3.51 vrs 3.54, p=0.56). However, we have found that the variable of the general state of health domain was statistically significantly higher in group B (2.66 vrs 2.89, p=0.001), while HAQ was statistically significantly higher in group A (1.19 vrs 1.07, p=0.018), as well as the duration of RA (6.25vrs 3.75 years, p=0.0006). Statistically significant correlation was found between HAQ and W2, disease duration and D3 in group A and DAS28CRP and D1, D2, W2 and HAQ and D1 and D2 in group B. CONCLUSION: These findings suggest that the inclusion of bDMARDs in the treatment regimen was overdue, with RA already advancing with developed functional disability, which prevented the achievement of the primary goals of treatment: low disease activity or remission and the improvement of patient's QOL.

Nail position has an influence on anterior knee pain after tibial intramedullary nailing.

Our aim was to determine the possible relationship between anterior knee pain (AKP) and nail position marked as a distance from tip of nail to tibial plateau (NP) and to the tuberositas tibiae (NT). Nail position has an influence on anterior knee pain after tibial intramedullary nailing. We evaluated postoperative outcome results of 50 patients in the last 3 years with healed fractures initially treated with intramedullary (IM) reamed nails with 2 or 3 interlocking screws on both parts of the nail and with the use of medial paratendinous incision for nail entry portal. Patients marked a point on the visual analog scale (VAS) that corresponded to the level of postoperative AKP felt. Two groups of patients were formed on the basis of AKP (pain level was neglected): groups A and B, with and without pain, respectively. The difference between the two groups concerning NP measurements was statistically significant (p < 0.05), but not concerning NT measurements at the p < 0.05 level. Patients were classified by pain with high accuracy (98%) according to a classification tree. Symptoms of AKP did not appear if the tip of the nail position was more than 6.0 mm from the NP and more than 2.6 mm from the NT. However, for better evaluation of these results it will be necessary to examine a larger number of postoperative patients with AKP.

Macro- and microelements in pumpkin seed oils: Effect of processing, crop season, and country of origin.

Macro- and microelements in the samples of virgin and cold pressed pumpkin seed oils produced in Croatia through two consecutive crop seasons were determined by inductively coupled plasma-optical emission spectroscopy (ICP-OES). Croatian oils were also compared to oils from Slovenia and Austria in order to assess differences in the element content. Magnesium, potassium, calcium, sodium, selenium, and iron were the dominant elements in all pumpkin seed oils. Their amounts together with barium, strontium, manganese, copper were up to ninefold higher (p ≤ 0.05) in virgin compared to cold pressed pumpkin seed oils. These differences occur due to the different processing conditions which include salt addition, heat treatment, and higher degree of equipment ware out during virgin pumpkin seed oil production. As the sodium level increases with the addition of salt, virgin pumpkin seed oil could be considered its hidden source and producers should pay attention to the amount added. Contents of cobalt, copper, selenium, and thallium significantly differed (p ≤ 0.05) between the two crop seasons. Principal component analysis revealed clear differences between samples with different origin that can be explained by the specifics in the production processes of each country. In comparison with Austrian and Slovenian, Croatian pumpkin seed oils had significantly lower contents of sodium, potassium, calcium, magnesium, and tin while bismuth and selenium were higher.