RESUMO
A number of studies investigating different aspects of IUCN endangered species, Aulopyge huegelii Heckel, 1843 (Dalmatian barbelgudgeon) biology have been conducted, but data on molecular genetics are lacking. The goal of this survey was to assess the genetic structure of the A. huegelii population from the Busko Blato reservoir, based on four mitochondrial DNA regions and five microsatellite loci. Excluding cytochrome b, more than one haplotype has been detected in all sequenced mtDNA regions, most of which had not been previously described. A total of seven composite haplotypes were detected. Nucleotide diversity was relatively low for all coding genes but slightly higher for the control region. Microsatellite analysis revealed a relatively high value of major allele frequency, lower values of observed and expected heterozygosity, as well as a moderately reduced number of alleles and genotypes in three of the five observed loci. Although with a clear trend of decline, the level of genetic diversity is still sufficient to ensure the subsistence of the population if the stressors are removed. Otherwise, the loss of heterozygosity will continue, possibly to the point of a complete eradication of the Dalmatian barbelgudgeon from the Busko Blato reservoir.
Assuntos
Cyprinidae/genética , DNA Mitocondrial/genética , Repetições de Microssatélites/genética , Animais , Bósnia e Herzegóvina , Espécies em Perigo de Extinção , Frequência do Gene , Variação Genética , Genética Populacional , Haplótipos , Perda de Heterozigosidade , ATPases Translocadoras de Prótons/genética , Análise de Sequência de DNARESUMO
This article presents a new approach to detect coiled coil and leucine zipper (L-Zip) motifs in protein sequences. The approach is based on protein scale calculation and sequence analysis. For this purpose, the wavelet-based local extrema extraction is employed, and window-based variations of local extrema afterward. This, in turn, provided a way to distinguish coiled coil subsequences and potential L-Zip motifs. The approach is validated on carefully chosen protein sequences that return inconclusive results within known frameworks for L-Zip detection, for example, 2ZIP. The results show that this new approach represents an improvement over previously presented approaches.