RESUMO
We describe the longitudinal kinetics of the serological response in COVID-19 recovered patients over a period of 14 months. The antibody kinetics in a cohort of 192 recovered patients, including 66 patients for whom follow-up serum samples were obtained at two to four clinic visits, revealed that RBD-specific antibodies decayed over the 14 months following the onset of symptoms. The decay rate was associated with the robustness of the response in that antibody levels that were initially highly elevated after the onset of symptoms subsequently decayed more rapidly. An exploration of the differences in the longitudinal kinetics between recovered patients and naïve vaccinees who had received two doses of the BNT162b2 vaccine showed a significantly faster decay in the naïve vaccinees, indicating that serological memory following natural infection is more robust than that following to vaccination. Our data highlighting the differences between serological memory induced by natural infection vs. vaccination contributed to the decision-making process in Israel regarding the necessity for a third vaccination dose.
Assuntos
COVID-19 , Anticorpos Neutralizantes , Anticorpos Antivirais , Vacina BNT162 , Humanos , Cinética , VacinaçãoRESUMO
MOTIVATION: A comprehensive characterization of the humoral response towards a specific antigen requires quantification of the B-cell receptor repertoire by next-generation sequencing (BCR-Seq), as well as the analysis of serum antibodies against this antigen, using proteomics. The proteomic analysis is challenging since it necessitates the mapping of antigen-specific peptides to individual B-cell clones. RESULTS: The PASA web server provides a robust computational platform for the analysis and integration of data obtained from proteomics of serum antibodies. PASA maps peptides derived from antibodies raised against a specific antigen to corresponding antibody sequences. It then analyzes and integrates proteomics and BCR-Seq data, thus providing a comprehensive characterization of the humoral response. The PASA web server is freely available at https://pasa.tau.ac.il and open to all users without a login requirement.
Assuntos
Anticorpos , Internet , Proteômica/métodos , Software , Animais , Anticorpos/sangue , Anticorpos/imunologia , Antígenos/imunologia , Linfócitos B/imunologia , Bases de Dados de Proteínas , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , CamundongosRESUMO
It is predicted that the antibiotic resistance crisis will result in an annual death rate of 10 million people by the year 2050. To grapple with the challenges of the impending crisis, there is an urgent need for novel and rapid diagnostic tools. In this study, we developed a novel monoclonal antibody-named mAb-EspB-B7-that targets the EspB protein, a component within the bacterial type 3 secretion system (T3SS), which is mainly expressed in Gram-negative pathogens and is essential for bacterial infectivity. We found that mAb-EspB-B7 has high affinity and specificity toward recombinant and native EspB proteins; is stable over a range of pH levels, temperatures, and salt concentrations; and retains its functionality in human serum. We identified the epitope for mAb-EspB-B7 and validated it by competitive enzyme-linked immunosorbent assay (ELISA). Since this epitope is conserved across several T3SS-harboring pathogens, mAb-EspB-B7 holds great potential for development as an active component in precise and rapid diagnostic tools that can differentiate between commensal and pathogenic bacterial strains. To this end, we integrated the well-characterized monoclonal antibody into an electrochemical biosensor and demonstrated its high specificity and sensitivity capabilities in detecting pathogenic bacterial T3SS-associated antigens as well as intact bacteria. We foresee that in the near future it will be possible to design and develop a point-of-care biosensor with multiplexing capabilities for the detection of a panel of pathogenic bacteria.
Assuntos
Anticorpos Monoclonais/sangue , Técnicas Biossensoriais , Técnicas Eletroquímicas , Bactérias Gram-Negativas/genética , Testes Imediatos , Sistemas de Secreção Tipo III/sangue , Ensaio de Imunoadsorção Enzimática , Bactérias Gram-Negativas/patogenicidade , Humanos , Concentração de Íons de Hidrogênio , Temperatura , Sistemas de Secreção Tipo III/genéticaRESUMO
The use of protein crystals as a source of nanoscale biotemplates has attracted growing interest in recent years owing to their inherent internal order. As these crystals are vulnerable to environmental changes, potential applications require their stabilization by chemical crosslinking. We have previously shown that such intermolecular chemical crosslinking reactions occurring within protein crystals are not random events, but start at preferred crosslinking sites imposed by the alignment of protein molecules and their packing within the crystalline lattice. Here we propose a new working hypothesis and demonstrate its feasibility in enabling us to extricate homogeneous populations of single protein molecules that display chemical point mutations or of dimers that show homogeneous chemical crosslinking, and that have the potential for isolation of higher structures. Characterization of the crosslinking mechanism and its end products opens the way to the potential retrieval of such specific modified/intermolecular crosslinked products simply by effecting partial crosslinking at identified preferred sites, followed by time-controlled arrest of the crosslinking reaction and dissolution of the crystals by medium exchange complemented by chromatographic purification.
Assuntos
Estabilidade Proteica , Proteínas/química , CristalizaçãoRESUMO
The lubrication properties of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) extended supported bilayers were studied and compared to those of surface-attached DSPC small unilamellar vesicles (liposomes) in order to elucidate the effect of phospholipid geometrical packaging on the lubrication and mechanical properties of these boundary layers. The topography and response to the nanoindentation of bilayer- and liposome-covered surfaces were studied by an atomic force microscope (AFM). In parallel, normal and shear (frictional) forces between two opposing surfaces bearing DSPC vesicles/bilayers across water were studied with the surface force balance (SFB). A correlation between nanomechanical performance in the AFM and stability and lubrication in the SFB was observed. Bilayers were readily punctured by the AFM tip and exhibited substantial hysteresis between approach and retraction curves, whereas liposomes were not punctured and exhibited purely elastic behavior. At the same time, SFB measurements showed that bilayers are less stable and less efficient lubricants compared to liposomes. Bilayers provided efficient lubrication with very low friction coefficients, 0.002-0.008 up to pressures of more then 50 atm. However, bilayers were less robust and tended to detach from the surface as a result of shear, leading to high friction for subsequent approaches at the same contact position. In contrast, liposomes showed reversible and reproducible behavior under shear and compression, exhibiting ultralow friction coefficients of µ ≈ 10(-4) for pressures as high as 180 atm. This is attributed to the increased mechanical stability of the self-closed, closely packed liposomes, which we believe results from the more defect-free nature of the finitely sized vesicles.
Assuntos
Lipossomos/química , Fosfatidilcolinas/química , Lubrificação , Microscopia de Força AtômicaRESUMO
BACKGROUND: The human milk antibody response following maternal immunization with the BNT162b2 mRNA vaccine is important for the protection of the infant during infancy. The vaccine-specific antibody response is still unclear at different stages of human milk production, as are the effects of maternal immunization timing on the robustness of the antibody response. OBJECTIVES: The study aimed to assess the antibody response (IgG/IgA/IgM) during various lactation stages and identify the best vaccination timing during pregnancy. METHODS: A prospective cohort study of 73 postpartum women who were administered the BNT162b2 COVID-19 mRNA vaccine during the second or third trimester of pregnancy were recruited. Statistical comparison was conducted using 16 human milk samples from a prepandemic control group. RESULTS: Excluding 11 women, the study included 62 lactating women who were administered the mRNA vaccine during the second or third trimester of pregnancy. A total of 149 samples of human milk were collected at different lactation stages. Our findings reveal that colostrum exhibits significantly higher levels of IgG (95% confidence interval [CI]: 1.3, 9.0; P = 0.023), IgA (95% CI: 55.98, 100.2; P = 0.0034), and IgM (95% CI: 0.03, 0.62; P < 0.0001) compared with mature milk IgG (95% CI: 0.25, 0.43), IgA (95% CI: 9.65, 13.74), IgM (95% CI: 0.03, 0.04). The timing of maternal immunization affected the antibody response. The level of IgA in mature milk was higher when immunization occurred in the second trimester (95% CI: 11.14, 19.66; P = 0.006) than in the third trimester (95% CI: 7.16, 11.49). Conversely, IgG levels in mature milk were higher when immunization occurred during the third trimester (95% CI: 0.36, 0.65; P < 0.0001) than in the second trimester (95% CI: 0.09, 0.38). CONCLUSIONS: Our study provides evidence that administering the mRNA vaccine to pregnant women during the second trimester increases vaccine-specific IgA levels during lactation. Considering the significance of human milk IgA in mucosal tissues and its prevalence throughout lactation, it is reasonable to recommend maternal immunization with the BNT162b2 mRNA vaccine during the second trimester. This trial was registered at the Helsinki Committee of the Tel Aviv Medical Center as clinical trial number 0172-TLV.
Assuntos
Vacinas contra COVID-19 , COVID-19 , Imunoglobulina A , Leite Humano , Feminino , Humanos , Lactente , Gravidez , Anticorpos Antivirais , Vacina BNT162 , COVID-19/prevenção & controle , Imunização , Imunoglobulina G , Imunoglobulina M , Lactação , Leite Humano/imunologia , Segundo Trimestre da Gravidez , Estudos Prospectivos , VacinaçãoRESUMO
Interactions in aqueous media between uniformly charged surfaces are well understood, but most real surfaces are heterogeneous and disordered. Here we show that two such heterogeneous surfaces covered with random charge domains experience a long-range attraction across water that is orders of magnitude stronger than van der Waals forces, even in the complete absence of any charge correlations between the opposing surfaces. We demonstrate that such strong attraction may arise generally, even for overall neutral surfaces, from the inherent interaction asymmetry between equally and between oppositely charged domains.
RESUMO
The role of B cells in the tumor microenvironment (TME) has largely been under investigated, and data regarding the antibody repertoire encoded by B cells in the TME and the adjacent lymphoid organs are scarce. Here, we utilized B cell receptor high-throughput sequencing (BCR-Seq) to profile the antibody repertoire signature of tumor-infiltrating lymphocyte B cells (TIL-Bs) in comparison to B cells from three anatomic compartments in a mouse model of triple-negative breast cancer. We found that TIL-Bs exhibit distinct antibody repertoire measures, including high clonal polarization and elevated somatic hypermutation rates, suggesting a local antigen-driven B-cell response. Importantly, TIL-Bs were highly mutated but non-class switched, suggesting that class-switch recombination may be inhibited in the TME. Tracing the distribution of TIL-B clones across various compartments indicated that they migrate to and from the TME. The data thus suggests that antibody repertoire signatures can serve as indicators for identifying tumor-reactive B cells.
Assuntos
Diversidade de Anticorpos , Subpopulações de Linfócitos B/imunologia , Cadeias Pesadas de Imunoglobulinas/genética , Linfócitos do Interstício Tumoral/imunologia , Neoplasias Mamárias Experimentais/imunologia , Receptores de Antígenos de Linfócitos B/imunologia , Neoplasias de Mama Triplo Negativas/imunologia , Microambiente Tumoral/imunologia , Animais , Células Sanguíneas/imunologia , Medula Óssea/patologia , Linhagem Celular Tumoral/transplante , Movimento Celular , Feminino , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Imunoglobulina G/genética , Imunoglobulina G/imunologia , Imunoglobulina M/genética , Imunoglobulina M/imunologia , Região Variável de Imunoglobulina/genética , Linfonodos/patologia , Neoplasias Mamárias Experimentais/patologia , Camundongos , Camundongos Endogâmicos BALB C , Especificidade de Órgãos , Receptores de Antígenos de Linfócitos B/genética , Hipermutação Somática de Imunoglobulina , Neoplasias de Mama Triplo Negativas/patologiaRESUMO
The importance of breastmilk in postnatal life lies in the strong association between breastfeeding and the reduction in the risk of infection and infection-related infant mortality. However, data regarding the induction and dynamics of breastmilk antibodies following administration of the Pfizer-BioNTech BNT162b2 COVID-19 mRNA vaccine is scarce, as pregnant and lactating women were not included in the initial vaccine clinical trials. Here, we investigate the dynamics of the vaccine-specific antibody response in breastmilk and serum in a prospective cohort of ten lactating women who received two doses of the mRNA vaccine. We show that the antibody response is rapid and highly synchronized between breastmilk and serum, reaching stabilization 14 days after the second dose. The response in breastmilk includes both IgG and IgA with neutralization capacity.
Assuntos
Aleitamento Materno , Vacinas contra COVID-19/genética , RNA Mensageiro/sangue , Adulto , Animais , Formação de Anticorpos/genética , Formação de Anticorpos/fisiologia , Vacina BNT162 , Feminino , Humanos , Leite/química , RNA Mensageiro/análise , Vacinas Sintéticas/uso terapêutico , Vacinas de mRNARESUMO
Antibodies are widely used in therapeutic, diagnostic, and research applications, and antibody derivatives such as F(ab')2 fragments are used when only a particular antibody region is required. F(ab')2 can be produced through antibody engineering, but some applications require F(ab')2 produced from an original formulated antibody or directly from a polyclonal antibody pool. The cysteine protease immunoglobulin-degrading enzyme (IdeS) from Streptococcus pyogenes digests immunoglobulin G (IgG) specifically and efficiently to produce F(ab')2 . Here we detail the production and purification of recombinant IdeS; its utilization to digest monoclonal or polyclonal antibodies to F(ab')2 fragments; and F(ab')2 purification through consecutive affinity chromatography steps. The resultant F(ab')2 exhibit high purity, retain antigen-binding functionality, and are readily utilizable in various downstream applications. © 2020 by John Wiley & Sons, Inc. Basic Protocol: Production and purification of F(ab')2 fragments from monoclonal and polyclonal antibodies using IdeS Alternate Protocol: Purification of polyclonal antigen-specific F(ab')2 fragments from human serum or secretions Support Protocol: Production and purification of IdeS.
Assuntos
Anticorpos Monoclonais/metabolismo , Fragmentos Fab das Imunoglobulinas/isolamento & purificação , Fragmentos Fab das Imunoglobulinas/metabolismo , Animais , Antígenos/imunologia , Cromatografia de Afinidade , Cisteína Proteases/isolamento & purificação , Cisteína Proteases/metabolismo , Escherichia coli/enzimologia , Escherichia coli/genética , Humanos , Fragmentos Fab das Imunoglobulinas/imunologia , Fragmentos Fc das Imunoglobulinas/metabolismo , Imunoglobulina G/metabolismo , Soro/química , Streptococcus pyogenes/enzimologiaRESUMO
Drugs formulated from monoclonal antibodies (mAbs) are clinically effective in various diseases. Repeated administration of mAbs, however, elicits an immune response in the form of anti-drug-antibodies (ADA), thereby reducing the drug's efficacy. Notwithstanding their importance, the molecular landscape of ADA and the mechanisms involved in their formation are not fully understood. Using a newly developed quantitative bio-immunoassay, we found that ADA concentrations specific to TNFα antagonists can exceed extreme concentrations of 1 mg/ml with a wide range of neutralization capacity. Our data further suggest a preferential use of the λ light chain in a subset of neutralizing ADA. Moreover, we show that administration of TNFα antagonists result in a vaccine-like response whereby ADA formation is governed by the extrafollicular T cell-independent immune response. Our bio-immunoassay coupled with insights on the nature of the immune response can be leveraged to improve mAb immunogenicity assessment and facilitate improvement in therapeutic intervention strategies.
Assuntos
Anticorpos Monoclonais/imunologia , Fator de Necrose Tumoral alfa/antagonistas & inibidores , Humanos , Imunoensaio , Fator de Necrose Tumoral alfa/imunologiaRESUMO
Strong nanofibers composed entirely of a model globular protein, namely, bovine serum albumin (BSA), were produced by electrospinning directly from a BSA solution without the use of chemical cross-linkers. Control of the spinnability and the mechanical properties of the produced nanofibers was achieved by manipulating the protein conformation, protein aggregation, and intra/intermolecular disulfide bonds exchange. In this manner, a low-viscosity globular protein solution could be modified into a polymer-like spinnable solution and easily spun into fibers whose mechanical properties were as good as those of natural fibers made of fibrous protein. We demonstrate here that newly formed disulfide bonds (intra/intermolecular) have a dominant role in both the formation of the nanofibers and in providing them with superior mechanical properties. Our approach to engineer proteins into biocompatible fibrous structures may be used in a wide range of biomedical applications such as suturing, wound dressing, and wound closure.
Assuntos
Nanoestruturas/química , Proteínas/química , Animais , Bovinos , Dissulfetos/química , Concentração de Íons de Hidrogênio , Teste de Materiais , Microscopia Eletrônica de Varredura , Nanopartículas/química , Nanotecnologia/métodos , Engenharia de Proteínas/métodos , Soroalbumina Bovina/química , Estresse Mecânico , Resistência à Tração , CicatrizaçãoRESUMO
Nanoparticles (NPs) which enter physiological fluids are rapidly coated by proteins, forming a so-called corona which may strongly modify their interaction with tissues and cells relative to the bare NPs. In this work the interactions between a living cell and a nano-object, and in particular the effect on this of the adsorption of serum proteins, are directly examined by measuring the forces arising as an Atomic Force Microscope tip (diameter 20 nm) - simulating a nano-object - approaches and contacts a cell. We find that the presence of a serum protein corona on the tip strongly modifies the interaction as indicated by pronounced increase in the indentation, hysteresis and work of adhesion compared to a bare tip. Classically one expects an AFM tip interacting with a cell surface to be repelled due to cell elastic distortion, offset by tip-cell adhesion, and indeed such a model fits the bare-tip/cell interaction, in agreement with earlier work. However, the force plots obtained with serum-modified tips are very different, indicating that the cell is much more compliant to the approaching tip. The insights obtained in this work may promote better design of NPs for drug delivery and other nano-medical applications.
Assuntos
Proteínas Sanguíneas/química , Fibroblastos/química , Nanopartículas/química , Animais , Bovinos , Adesão Celular , Módulo de Elasticidade , Microscopia de Força Atômica , Cultura Primária de CélulasRESUMO
BACKGROUND: Whether outcome from in-hospital cardiopulmonary resuscitation (CPR) is poorer when it occurs during the night remains controversial. This study examined the relationship between CPR during the various hospital shifts and survival to discharge. METHODS: CPR attempts occurring in a tertiary hospital with a dedicated, certified resuscitation team were recorded prospectively (Utstein template guidelines) over 24 months. Medical records and patient characteristics were retrieved from patient admission files. RESULTS: Included were 174 in-hospital cardiac arrests; 43%, 32% and 25% in morning evening and night shifts, respectively. Shift populations were comparable in demographic and treatment related variables. Asystole (p < 0.01) and unwitnessed arrests (p = 0.05) were more common during the night. Survival to discharge was poorer following night shift CPR than following morning and evening shift CPR (p = 0.04). When asystole (being synonymous with death) was excluded from the analysis, the odds of survival to discharge was not higher for witnessed compared to unwitnessed arrest but was 4.9 times higher if the cardiac arrest did not occur during the night shift (p = 0.05, logistic regression). The relative risk of eventual in-hospital death for patients with return of spontaneous circulation (ROSC) following night shift resuscitation was 1.9 that of those with ROSC following morning or evening resuscitation (Cox regression). CONCLUSIONS: Although unwitnessed arrest is more prevalent during night shift, resuscitation during this shift is associated with poorer outcomes independently of witnessed status. Further research is required into the causes for the increased mortality observed after night shift resuscitation.
Assuntos
Reanimação Cardiopulmonar/normas , Parada Cardíaca/mortalidade , Hospitalização , Idoso , Circulação Sanguínea/fisiologia , Feminino , Humanos , Masculino , Estudos Prospectivos , Análise de Regressão , TempoRESUMO
Phosphatidylcholine (PC) vesicles have been shown to have remarkable boundary lubricating properties under physiologically-high pressures. Here we carry out a systematic study, using a surface force balance, of the normal and shear (frictional) forces between two opposing surfaces bearing different PC vesicles across water, to elucidate the origin of these properties. Small unilamellar vesicles (SUVs, diameters < 100 nm) of the symmetric saturated diacyl PCs DMPC (C(14)), DPPC (C(16)) and DSPC (C(18)) attached to mica surfaces were studied in their solid-ordered (SO) phase on the surface. Overall liposome lubrication ability improves markedly with increasing acyl chain length, and correlates strongly with the liposomes' structural integrity on the substrate surface: DSPC-SUVs were stable on the surface, and provided extremely efficient lubrication (friction coefficient µ ≈ 10(-4)) at room temperature at pressures up to at least 18 MPa. DMPC-SUVs ruptured following adsorption, providing poor high-pressure lubrication, while DPPC-SUVs behavior was intermediate between the two. These results can be well understood in terms of the hydration-lubrication paradigm, but suggest that an earlier conjecture, that highly-efficient lubrication by PC-SUVs depended simply on their being in the SO rather than in the liquid-disordered phase, should be more nuanced. Our results indicate that the resistance of the SUVs to mechanical deformation and rupture is the dominant factor in determining their overall boundary lubrication efficiency in our system.
Assuntos
Lipossomos/química , Lubrificação , Fosfatidilcolinas/química , 1,2-Dipalmitoilfosfatidilcolina/química , Adsorção , Silicatos de Alumínio , Microscopia Crioeletrônica , Dimiristoilfosfatidilcolina/química , Fricção , Microscopia de Força Atômica , Resistência ao Cisalhamento , Propriedades de Superfície , Fatores de TempoRESUMO
Wolman disease is a rapidly fatal lysosomal storage disease caused by the complete absence of lysosomal acid lipase activity. We report the cure of an infant with Wolman disease following transplantation of unrelated HLA-mismatched umbilical cord blood-derived stem cells. Umbilical cord blood was chosen as the stem-cell source because of its immediate availability and reduced tendency to cause graft-versus-host disease. The transplantation resulted in restoration of normal acid lipase levels before the onset of permanent end-organ damage. Four years after transplantation, the patient is thriving and has normal levels of acid lipase in peripheral blood cells. To our knowledge, this is the first report of a successful unrelated cord blood transplant in a patient with Wolman disease. Umbilical cord stem cells transplantation can restore acid lipase levels in Wolman disease, and if performed early, can cure the disease.
Assuntos
Transplante de Células-Tronco de Sangue do Cordão Umbilical , Doença de Wolman/terapia , Encéfalo/anatomia & histologia , Desenvolvimento Infantil , Pré-Escolar , Feminino , Doença Enxerto-Hospedeiro/prevenção & controle , Humanos , Lactente , Imageamento por Ressonância Magnética , Transplante HomólogoRESUMO
Screening of mutant libraries for in vitro enzyme evolution is carried out primarily by physical separation of the cells, followed by growth of individual clones and screening of biocatalytic activity on the basis of color or fluorescence signal development. Currently, most frequently employed methods are labor-intensive or require robotic equipment, resulting in screening limited to a relatively small fraction of the potential inherent in a given library. In this study we present a design, development, and feasibility demonstration of a new screening approach, providing convenient handling of large libraries consisting of 106 to 107 clones and screening based on a simultaneous enzymatic assay with commercially available substrates. This new screening method is based on the "cell immobilized on adsorbed bead" approach: the cell population to be screened is mixed with an excess of medium pre-equilibrated polyacrylamide beads, chemically derivatized to affect quantitative cell immobilization by adsorption. The resulting bead population, comprising of single cell on a bead or blank beads, is then immobilized on a solid glass support. After removal of the freely flowing liquid, the cells immobilized on the adsorbed beads are allowed to grow into microcolonies, utilizing the medium retained within the supporting hydrogel matrix. These colonies are subsequently equilibrated with chromogenic or fluorogenic substrate and screening is affected under a stereomicroscope, resulting in readily retrieved of the most active colonies. This technique may be particularly useful when the screened mutants are expressed and displayed on the cell surface, providing an active and homogeneous "naturally immobilized" enzyme population with minimal substrate diffusion limitations.
Assuntos
Escherichia coli/isolamento & purificação , Escherichia coli/metabolismo , Biblioteca de Peptídeos , Proteínas/análise , Proteínas/química , beta-Lactamases/análise , beta-Lactamases/química , Adsorção , Células Imobilizadas/citologia , Células Imobilizadas/metabolismo , Escherichia coli/citologia , Estudos de Viabilidade , Citometria de Fluxo/métodos , Microesferas , Proteínas/metabolismo , beta-Lactamases/biossíntese , beta-Lactamases/genéticaRESUMO
The electrospinning process was used successfully to embed single-walled carbon nanotubes (SWCNTs) in a poly(ethylene oxide) (PEO) matrix, forming composite nanofibers. Initial dispersion of SWCNTs in water was achieved by the use of an amphiphilic alternating copolymer of styrene and sodium maleate. The resulting dispersions were stable, having a dark, smooth, ink-like appearance. For electrospinning, the dispersions were mixed with PEO solution in an ethanol/water mixture. The distribution and conformation of the nanotubes in the nanofibers were studied by transmission electron microscopy (TEM). Oxygen plasma etching was used to expose the nanotubes within the nanofibers to facilitate direct observation. Nanotube alignment within the nanofibers was shown to depend strongly on the quality of the initial dispersions. Well-dispersed and separated nanotubes were embedded in a straight and aligned form, while entangled nonseparated nanotubes were incorporated as dense aggregates. X-ray diffraction demonstrated a high degree of orientation of the PEO crystals in the electrospun nanofibers with embedded SWCNTs. This result is in pronounced distinction to the detrimental effect of incorporation of multiwalled carbon nanotubes on polymer orientation in electrospun nanofibers, as reported previously.