RESUMO
Kaixinsan powder (KXS), a classic prescription of traditional Chinese Medicine (TCM), is widely used in the treatment of depression, but its mechanism remains unclear. The network pharmacology method was used to constructe the "herb-component-target" network, and elucidated KXS potential mechanisms of action in the treatment of depression. Moreover, molecular docking was applied to valid the important interactions between the ingredients and the target protein. The "herb-component-target" network indicated that the ingredients of Girinimbin, Gomisin B and Asarone, and the protein targets of ESR, AR and NR3C1 mostly contribute to the antidepressant effect of KXS. KEGG pathway analysis highlighted the most significant pathways associated with depression treatment, including neuroactive ligand-receptor interaction pathway, serotonergic synapse pathway, PI3K-Akt signaling pathway and MAPK signaling pathway. Go enrichment analysis indicated that the mechanism of KXS in treating depression was involved in the biological process of GPCR signal transduction, hormone metabolism and nerve cell apoptosis. Moreover, molecular docking results showed that Polygalaxanthone III, Girinimbine and Pachymic acid performed greater binding ability with key antidepressant target 5-HTR. In conclusion, this study preliminarily revealed key active components in KXS, including Gomisin B, Asarone, Ginsenoside Rg1, Polygalaxanthone III and Pachymic acid, could interact with multiple targets (5-HTR, DR, ADRA, AR, ESR, NR3C1) and modulate the activation of multiple pathways (Neuroactive ligand -receptor interaction pathway, serotonergic synapse pathway, PI3K-Akt signaling pathway and MAPK signaling pathway).
Assuntos
Depressão , Fosfatidilinositol 3-Quinases , Pós , Simulação de Acoplamento Molecular , Depressão/tratamento farmacológico , Ligantes , Proteínas Proto-Oncogênicas c-akt , Antidepressivos/farmacologia , Antidepressivos/uso terapêuticoRESUMO
A rapid, simple, and efficient sample extraction method based on micro-matrix-solid-phase dispersion (micro-MSPD) was applied to the extraction of polyphenols from pomegranate peel. Five target analytes were determined by ultra-HPLC coupled with Q-TOF/MS. Carbon molecular sieve (CMS) was firstly used as dispersant to improve extraction efficiency in micro-MSPD. The major micro-MSPD parameters, such as type of dispersant, amount of dispersant, grinding time, and the type and the volume of elution solvents, were studied and optimized. Under optimized conditions, 26 mg of pomegranate peel was dispersed with 32.5 mg of CMS, the grinding time was selected as 90 s, the dispersed sample was eluted with 100 µL of methanol. Results showed that the proposed method was of good linearity for concentrations of analytes against their peak areas (coefficient of determination r2 > 0.990), the LOD was as low as 3.2 ng/mL, and the spiking recoveries were between 88.1 and 106%. Satisfactory results were obtained for the extraction of gallic acid, punicalagin A, punicalagin B, catechin, and ellagic acid from pomegranate peel sample, which demonstrated nice reliability and high sensitivity of this approach.
Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Lythraceae/química , Espectrometria de Massas/métodos , Polifenóis/isolamento & purificação , Extração em Fase Sólida/métodos , Carbono/química , Limite de Detecção , Modelos Lineares , Extratos Vegetais/química , Polifenóis/análise , Polifenóis/química , Reprodutibilidade dos TestesRESUMO
Nanotechnology shows great potential for producing food with higher quality and better taste through including new additives, improving nutrient delivery, and using better packaging. However, lack of investigations on safety issues of nanofood has resulted in public fears. How to characterize engineered nanomaterials in food and assess the toxicity and health impact of nanofood remains a big challenge. Herein, a facile and highly reliable separation method of TiO2 particles from food products (focusing on sugar-coated chewing gum) is reported, and the first comprehensive characterization study on food nanoparticles by multiple qualitative and quantitative methods is provided. The detailed information on nanoparticles in gum includes chemical composition, morphology, size distribution, crystalline phase, particle and mass concentration, surface charge, and aggregation state. Surprisingly, the results show that the number of food products containing nano-TiO2 (<200 nm) is much larger than known, and consumers have already often been exposed to engineered nanoparticles in daily life. Over 93% of TiO2 in gum is nano-TiO2 , and it is unexpectedly easy to come out and be swallowed by a person who chews gum. Preliminary cytotoxicity assays show that the gum nano-TiO2 particles are relatively safe for gastrointestinal cells within 24 h even at a concentration of 200 µg mL(-1) . This comprehensive study demonstrates accurate physicochemical property, exposure, and cytotoxicity information on engineered nanoparticles in food, which is a prerequisite for the successful safety assessment of nanofood products.
Assuntos
Carboidratos , Goma de Mascar , Aditivos Alimentares , Nanopartículas Metálicas/toxicidade , Titânio/toxicidade , Sistema Digestório/química , Humanos , Nanopartículas Metálicas/análise , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Titânio/análiseRESUMO
A green and efficient microemulsion liquid chromatographic (MELC) method using fatty acid as co-surfactant and electrochemical detection was established and validated for the determination of four caffeoylquinic acid isomers and caffeic acid in honeysuckle samples. The influences of each individual component within the isocratic oil-in-water (O/W) microemulsion mobile phase were systematically investigated, such as the type and concentration of co-surfactant, concentration of sodium dodecyl sulphate (SDS), organic modifier addition, type and concentration of oil phase, pH and detection voltage. Results indicated that excellent resolution was achieved using 3.0% w/v of propionic acid, 0.5% w/v of ethyl acetate, 1.0% w/v of SDS, 5% w/v acetonitrile, 90.5% v/v of water and 25 mM sodium dihydrogen phosphate at pH = 3 as microemulsion mobile phase and 0.8 V as the optimal voltage value. Under the optimal condition, analytical performance of developed method was evaluated. The detection limits were below 17.3 ng/mL and intra-day and inter-day precisions by relative standard deviations (RSD%) were between 0.5% and 3.6%. Satisfactory recovery (in the range of 83.8-109.1%) with good repeatability lower than 4.7% (n = 3) was obtained. Therefore, the developed O/W MELC method was rapid, precise and accurate for simultaneous determination of neochlorogenic acid, chlorogenic acid, isochlorogenic acid A and isochlorogenic acid C in honeysuckle samples, with contents of 2.6, 28.7, 18.1 and 5.2 mg/g, respectively.
Assuntos
Ácidos Cafeicos/análise , Cromatografia Líquida/métodos , Ácidos Graxos/química , Lonicera/química , Ácido Quínico/análogos & derivados , Tensoativos/química , Ácidos Cafeicos/química , Emulsões , Isomerismo , Limite de Detecção , Estrutura Molecular , Ácido Quínico/análise , Ácido Quínico/químicaRESUMO
A Newcastle disease virus (NDV) strain, APMV-1/Chicken/China(SC)/PT3/2016, was isolated from asymptomatic chickens at a breeding farm in China. The PT3 strain has a genome length of 15,198 nucleotides and is classified as subgenotype 1b of class I. Pathogenicity tests demonstrated that PT3 is a lentogenic strain.
RESUMO
The fast growing applications of ZnO nanoparticles (NPs) in food sector and other fields enhance the exposure possibility of human beings to ZnO NPs including via oral administration route. Although the oral toxicity of ZnO NPs has been studied, most of the research was performed on the normal animal models. Therefore, the understanding of the biological consequence of ZnO NPs on the population with diseases, especially gastrointestinal disease, is extremely limited. In this study, a mice model of inflammatory bowel disease (IBD) induced by indomethacin has been developed to comprehensively investigated the bioeffects of ZnO NPs on the specific population. The effect of the intestinal inflammation/injury on the distribution and toxicity of orally administrated ZnO NPs (nZnO, 20â¯nmâ¯×â¯100â¯nm and mZnO, â¼200â¯nm) in mice were analyzed. The results showed that there was a difference in the distribution of Zn and the essential trace elements (Fe and Cu) between the IBD mice and the normal mice. We also observed an obvious size effect. Higher hepatic Zn was detected in the IBD mice post-exposure to ZnO NPs, especially bigger ZnO NPs. In addition, the histopathological examination of main organs and biological parameters analysis showed that ZnO NPs caused slight toxicity to the liver and kidneys in the IBD mice. Our findings highlight the importance of the health status of animals on the bioeffects of nanomaterials.
Assuntos
Doenças Inflamatórias Intestinais/metabolismo , Intestinos/efeitos dos fármacos , Nanopartículas Metálicas/toxicidade , Óxido de Zinco/toxicidade , Administração Oral , Animais , Cobre/metabolismo , Modelos Animais de Doenças , Indometacina , Doenças Inflamatórias Intestinais/induzido quimicamente , Doenças Inflamatórias Intestinais/patologia , Mucosa Intestinal/metabolismo , Intestinos/patologia , Ferro/metabolismo , Rim/efeitos dos fármacos , Rim/metabolismo , Rim/patologia , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Masculino , Camundongos Endogâmicos ICR , Distribuição Tecidual , Óxido de Zinco/administração & dosagem , Óxido de Zinco/metabolismoRESUMO
A simple and effective method of miniaturized solid-phase extraction (mini-SPE) was developed for the simultaneous purification and enrichment of macrolide antibiotics (MACs) (i.e. azithromycin, clarithromycin, erythromycin, lincomycin and roxithromycin) from honey and skim milk. Mesoporous MCM-41 silica was synthesized and used as sorbent in mini-SPE. Several key parameters affecting the performance of mini-SPE procedure were thoroughly investigated, including sorbent materials, amount of sorbent and elution solvents. Under the optimized condition, satisfactory linearity (r2â¯>â¯0.99), acceptable precision (RSDs, 0.3-7.1%), high sensitivity (limit of detection in the range of 0.01-0.76⯵g/kg), and good recoveries (83.21-105.34%) were obtained. With distinct advantages of simplicity, reliability and minimal sample requirement, the proposed mini-SPE procedure coupled with ultrahigh performance liquid chromatography and quadrupole time-of-flight tandem mass spectrometry could become an alternative tool to analyze the residues of MACs in complex food matrixes.
Assuntos
Análise de Alimentos/métodos , Mel/análise , Macrolídeos/isolamento & purificação , Leite/química , Dióxido de Silício/química , Extração em Fase Sólida/métodos , Animais , Antibacterianos/análise , Antibacterianos/isolamento & purificação , Bovinos , Cromatografia Líquida de Alta Pressão , Limite de Detecção , Macrolídeos/análise , Reprodutibilidade dos Testes , Solventes/análise , Espectrometria de Massas em TandemRESUMO
In this study, dispersive micro solid phase extraction (DMSPE) combined with ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry method was established to extract and determine sudan dyes and their metabolites in real samples. The crown ether microfunctionalized multi-walled carbon nanotubes (MWCNTs) was applied as the sorbent in DMSPE procedure. Several experimental parameters that can effect the extraction performance of the DMSPE method were investigated separately by a univariate method. The validation data showed that the limits of detection were in the range of 0.084-13.13⯵g/kg, the mean recoveries were ranged from 80.15 to 103.58% for six samples. Compared with other published methods, the proposed method was more effective, more time-saving and more eco-friendly. Finally, the developed method was successfully applied to enrich and detect sudan dyes and their metabolites in real samples.
Assuntos
Compostos Azo/isolamento & purificação , Cromatografia Líquida de Alta Pressão/métodos , Corantes/isolamento & purificação , Análise de Alimentos/métodos , Microextração em Fase Sólida/métodos , Espectrometria de Massas em Tandem/métodos , Compostos Azo/análise , Corantes/análise , Éteres de Coroa/química , Limite de Detecção , Nanotubos de Carbono/químicaRESUMO
Avian infectious bronchitis (IB) is a highly contagious disease, and hazardous to the poultry industry. Immune failure often occurs due to the emergence of new serotypes or field strains antigenically different from the vaccine strains. To prepare a candidate vaccine against the prevalent avian infectious bronchitis virus (IBV) in China, the GI-19/QX-like field isolate Sczy3 was selected as the progenitor strain and attenuated via passaging in chicken embryo kidney (CEK) cells for 100 times. The 100th generation of CEK-adapted strain, designated SczyC100, was safe to use on one-day old specific pathogen-free (SPF) chicken as determined by pathogenicity and virulence reversion test. The efficacies of SczyC100 and two commonly used commercial vaccines (H120 and 4/91) against prevalent GI-19/QX and GI-7/TWI type virulent strains were evaluated. Sczy3C100 effectively reduced the morbidity, mortality, mean lesion scores (MLSs), and viral load of trachea of chickens challenged by GI-19/QX and GI-7/TWI strains. CEK-adapted SczyC100 is therefore a potential vaccine candidate for the control of IB in China.
Assuntos
Infecções por Coronavirus/veterinária , Vírus da Bronquite Infecciosa/imunologia , Doenças das Aves Domésticas/prevenção & controle , Vacinas Virais/imunologia , Animais , Linhagem Celular , Galinhas , China , Infecções por Coronavirus/imunologia , Infecções por Coronavirus/patologia , Infecções por Coronavirus/prevenção & controle , Células Epiteliais/virologia , Vírus da Bronquite Infecciosa/crescimento & desenvolvimento , Vírus da Bronquite Infecciosa/patogenicidade , Doenças das Aves Domésticas/imunologia , Inoculações Seriadas , Análise de Sobrevida , Traqueia/virologia , Vacinas Atenuadas/administração & dosagem , Vacinas Atenuadas/imunologia , Vacinas Atenuadas/isolamento & purificação , Carga Viral , Vacinas Virais/administração & dosagem , Vacinas Virais/isolamento & purificação , Virulência , Cultura de Vírus/métodosRESUMO
Mycotoxins are a group of secondary fungi metabolites present in foods that cause adverse effects in humans and animals. The objective of this study was to develop and validate a reliable and sensitive method to determine the presence of fumonisin B1, aflatoxin B1, ochratoxin B, T-2 toxin, ochratoxin A and zearalenone. A rapid, effective process, which involves microwave-assisted dispersive micro-solid phase extraction (MA-d-µ-SPE), has been proposed for the extraction and detection of 6 mycotoxins in peach seed, milk powder, corn flour and beer sample matrixes, for subsequent analysis by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF/MS). Several experimental parameters (type of dispersant, concentration of dispersant, vortex time, type of desorption solvent and pH) affecting the extraction efficiency were systematically studied and optimized. The optimum extraction conditions involved immersing 2.5⯵g/mL of nano zirconia (as dispersant) in a 5â¯mL sample solution. After 2â¯min of extraction by vigorous shaking, the target analytes were desorbed by 100⯵L of chloroform at pH 4.5. The results indicated good linearity in the range of 0.0074-3.6⯵g/mL (râ¯≥â¯0.9982), low limits of detection (0.0036-0.033⯵g/kg for solid samples and 0.0022-0.017â¯ng/mL for beer), acceptable reproducibility (relative standard deviation (RSD%) 2.08-2.76% for retention time and 3.51-4.59% for peak area, nâ¯=â¯3), and satisfactory spiked recoveries (84.27-104.96%) for studied mycotoxins in sample matrixes, which demonstrated that MA-d-µ-SPE coupled with UHPLC-Q-TOF/MS is a useful tool for analysis of multi-mycotoxin.
Assuntos
Análise de Alimentos/métodos , Nanopartículas Metálicas/química , Micro-Ondas , Micotoxinas/análise , Micotoxinas/isolamento & purificação , Microextração em Fase Sólida/métodos , Zircônio/química , Cerveja/análise , Cromatografia Líquida de Alta Pressão/métodos , Humanos , Limite de Detecção , Espectrometria de Massas em Tandem/métodosRESUMO
A simple, rapid and eco-friendly approach based on matrix solid-phase dispersion microextraction (MSPDM) followed by ultrahigh performance liquid chromatography coupled with electrochemical detection (UHPLC-ECD) was presented for the microextraction and determination of six phenolic acids in a plant preparation (Danshen tablets). The parameters that influenced the extraction performance of phenolic acids were investigated and optimized. The optimal MSPDM conditions were determined as follows: sorbent, using graphene nanoplatelets with sample/sorbent ratio of 1:1, grinding time set at 60 s, and 0.2 mL of water as elution solvent. Under the optimum conditions, the validation experiments indicated that the proposed method exhibited good linearity (r2 ≥ 0.9991), excellent precision (RSD ≤ 4.57%), and satisfactory recoveries (82.34-98.34%). The limits of detection were from 1.19 to 4.62 ng/mL for six phenolic acids. Compared with other reported methods, this proposal required less sample, solvent and extraction time. Consequently, the proposed method was successfully used to the extraction and determination of phenolic acids in Danshen tablets.
RESUMO
An environmentally friendly ionic liquid-in-water (IL/W) microemulsion was established and applied as mobile phase in microemulsion liquid chromatography (MELC) with ultraviolet (UV) detection or electrochemical detector (ECD) for analysis of phenolic compounds in real samples. The optimal condition of the method was using the best composition of microemulsion (0.2% w/v [HMIM]PF6, 1.0% w/v SDS, 3.0% w/v n-butanol, 95.8% v/v water, pH 2.5) with UV detection. The validation results indicated that the method provided high degree of sensitivity, precision and accuracy with the low limit of detections ranged from 17.9-238ng/mL, satisfactory mean recovery values in the range of 80.1-105% and good linearity (r2>0.9994). Additionally, this method exhibited high selectivity and resolution for the analytes and was more eco-friendly compared with traditional MELC method. Consequently, the established IL/W MELC method was successfully applied to simultaneously separate and determine target compounds in Danshen sample and its preparation.
Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/análise , Hidroxibenzoatos/análise , Líquidos Iônicos/química , Salvia miltiorrhiza/química , Cromatografia Líquida de Alta Pressão/instrumentação , Eletroquímica/instrumentação , Eletroquímica/métodos , Emulsões/química , Fenantrolinas/análise , Espectrofotometria Ultravioleta/instrumentação , Espectrofotometria Ultravioleta/métodos , Água/químicaRESUMO
An ionic liquid-(IL) based micellar extraction combined with microcrystalline cellulose- (MCC) assisted dispersive micro solid-phase extraction method was developed to extract phenolic compounds from propolis. A total of 20 target compounds were identified by ultra-high- performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. The main extraction parameters were optimized and included the ultrasonic power, ultrasonic time, sample pH, type of IL, the concentration of [C12mim]Br, extraction time, concentration of MCC, type of sorbent and type of elution solvents. Under the optimum conditions, the proposed method exhibited good linearities (r2 ≥ 0.999) for all plant phenolic compounds with the lower limits of detection in the range of 0.21-0.41 ng/mL. The recoveries ranged from 82.74% to 97.88% for pinocembrin, chrysin and galangin. Compared with conventional solvent extraction, the present method was simpler and more efficient and required less organic solvent and a shorter extraction time. Finally, the methodology was successfully used for the extraction and enrichment of phenolic compounds in propolis.
Assuntos
Celulose/química , Líquidos Iônicos/química , Microextração em Fase Líquida/métodos , Micelas , Fenóis/isolamento & purificação , Própole/química , Ondas Ultrassônicas , Adsorção , Fenóis/químicaRESUMO
Two kinds of extraction methods ultrasonic-assisted micellar extraction (UAME) and microwave-assisted micellar extraction (MAME) coupled with ultra-high performance liquid chromatography with ultraviolet detector (UHPLC-UV) were developed and evaluated for extraction and determination of zingerone, 6-gingerol, 8-gingerol, 6-shogaol and 10-gingerol in Rhizoma Zingiberis and Rhizoma Zingiberis Preparata. A biosurfactant, hyodeoxycholic acid sodium salt, was used in micellar extraction. Several experimental parameters were studied separately by a univariate method. The result indicated that the MAME was more efficient than UAME. The optimal conditions of MAME were as follows: 100mM of hyodeoxycholic acid sodium salt was used as surfactant, the irradiation time was set at 10s and the extraction temperature was set at 60°C. The validation results indicated that the limits of detection were in the range of 3.80-8.11ng/mL. The average recoveries were in the range of 87.32-103.12% for the two samples at two spiking levels. Compared with other reported methods, the proposed MAME-UHPLC-UV method was more effective, quicker (10s) and more eco-friendly.
Assuntos
Catecóis/isolamento & purificação , Técnicas de Química Analítica/métodos , Cromatografia Líquida de Alta Pressão , Álcoois Graxos/isolamento & purificação , Zingiber officinale/química , Limite de Detecção , Micelas , Micro-Ondas , Rizoma/química , Tensoativos/química , Temperatura , UltrassomRESUMO
Zwitterionic surfactant, used as extractant in microwave-assisted extraction (MAE) was investigated for the first time to extract organic and inorganic iodines from kelp samples. Optimized conditions for the MAE were 200W of microwave irradiation power, 100°C of extraction temperature, 10min of microwave irradiation time, 1g of sample, and 20mL of solvent volume. Ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) was used for the quantitative and qualitative analyses of the iodines. Under the optimum experimental conditions, KI, MIT and DIT were identified in kelp samples, the limits of detection of these analytes were ranged between 3.39 and 6.31ng/mL. The recoveries for spiked samples obtained from different areas were all higher than 92.48%. Compared with the ultrasound-assisted extraction, the proposed method is faster and more effective. Thus, the combination of zwitterionic surfactant-MAE and UHPLC-Q-TOF/MS made up a simple, rapid and effective approach for extraction and determination of iodine compounds in complex seaweed materials.
Assuntos
Fracionamento Químico/métodos , Iodo/isolamento & purificação , Extratos Vegetais/isolamento & purificação , Alga Marinha/química , Tensoativos/química , Verduras/química , Fracionamento Químico/instrumentação , Cromatografia Líquida de Alta Pressão/métodos , Iodo/química , Espectrometria de Massas/métodos , Micelas , Micro-Ondas , Extratos Vegetais/químicaRESUMO
This study presents an effective method of using miniaturized matrix solid phase dispersion (MSPD) for the microextraction of inorganic iodine and iodinated amino acids from seaweed samples. Quantification of the target analytes was performed by ultrahigh performance liquid chromatography with UV detection. Molecular sieve (SBA-15) was chosen as the dispersing adsorbent with an ionic liquid (1-dodecyl-3-methylimidazolium bromide) as the elution solvent. The experimental conditions for the MSPD, such as the type of sorbent, ratio of sorbent to sample, type and concentration of the elution solvent, and grinding time were evaluated and optimized. Under the final working conditions, good recoveries were obtained in the range of 86.5-95.4%, with relative standard deviation values below 6.0% in all cases. The limits of detection and limits of quantitation were in the ranges of 3.7-16.7ng/mL and 12.4ng/mL, respectively. Compared with common ultrasound assisted extraction, the advantages of this green approach are low consumption of the sorbent and solvent, short extraction time and good selectivity, even in complicated matrices. The proposed SBA-15-based MSPD method was successfully applied to the microextraction of potassium iodide, 3-iodo-l-tyrosine, and 3,5-diiodo-l-tyrosine from kelp, nori and undaria pinnatifida, respectively.
Assuntos
Aminoácidos/isolamento & purificação , Iodo/isolamento & purificação , Líquidos Iônicos/química , Extração em Fase Sólida/métodos , Adsorção , Calibragem , Cromatografia Líquida de Alta Pressão , Imidazóis/química , Limite de Detecção , Porosidade , Reprodutibilidade dos Testes , Alga Marinha/química , Alga Marinha/ultraestrutura , Dióxido de Silício/química , Solventes/química , Espectroscopia de Infravermelho com Transformada de Fourier , Fatores de TempoRESUMO
The aim of this study was to decipher the molecular epidemiological and antigenic characteristics of infectious bronchitis virus strains (IBVs) isolated in recent years in southwestern China. A total of 24 field strains were isolated from diseased chickens between 2012 and 2016. Phylogenetic analysis based on S1 nucleotide sequences showed that 16 of the 24 isolates were clustered into four distinct genotypes: QX (37.5%), TW (16.7%, TWI and TWII), Mass (8.3%), and J2 (4.2%). The QX genotype was still the prevalent genotype in southwestern China. Recombination analysis of the S1 subunit gene showed that eight of the 24 field strains were recombinant variants that originated from field strains and vaccine strains. A new potential recombination hotspot [ATTTT(T/A)] was identified, implying that recombination events may become more and more common. The antigenicity of ten IBVs, including seven field strains and commonly used vaccine strains, were assayed with a viral cross-neutralization assay in chicken embryonated kidney cells (CEK). The results showed that the ten IBVs could be divided into four serotypes (Massachusetts, 793B, Sczy3, and SCYB). Sczy3 and 793B were the predominant serotypes. Six of the seven field isolates (all except for cK/CH/SCYB/140913) cross-reacted well with anti-sera against other field strains. In conclusion, the genetic and antigenic features of IBVs from southwestern China in recent years have changed when compared to the previous reports. The results could provide a reference for vaccine development and the prevention of infectious bronchitis in southwestern China.
Assuntos
Infecções por Coronavirus/virologia , Vírus da Bronquite Infecciosa/classificação , Vírus da Bronquite Infecciosa/genética , Animais , Antígenos Virais/genética , Galinhas/virologia , China , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/imunologia , Genótipo , Vírus da Bronquite Infecciosa/imunologia , Rim/virologia , Pulmão/virologia , Epidemiologia Molecular , FilogeniaRESUMO
Nanotechnology has stepped into the food industry, from the farm to the table at home, in order to improve the taste and nutritional value, extend the shelf-life and monitor the food quality. In fact, as consumers we have already been in contact, via oral exposure, with a number of food products containing engineered nanomaterials (ENMs) more often than most people think. However, the fate of ENMs after entering the GI tract of the human body is not yet clearly understood. Hence, the related safety issue is raised, and attracts much attention and wide debate from the public, even including protest demonstrations against nanotechnology in food. In this review, we summarize the up-to-date information about the characterization and safety evaluation of common inorganic ENMs (with a focus on silver, titanium dioxide, silica and zinc oxide nanoparticles) in food. Based on the literature, a whole scenario of the safety issue of these ENMs in food and an outlook on the future studies are given.