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The prevention of fungal proliferation in postharvest grains is critical for maintaining grain quality and reducing mycotoxin contamination. Fumigation with natural gaseous fungicides is a promising and sustainable approach to protect grains from fungal spoilage. In this study, the antifungal activities of (E)-2-alkenals (C5-C10) on Aspergillus flavus were tested in the vapor phase, and (E)-2-heptenal showed the highest antifungal activity against A. flavus. (E)-2-Heptenal completely inhibited A. flavus growth at 0.0125 µL/mL and 0.2 µL/mL in the vapor phase and liquid contact, respectively. (E)-2-Heptenal can disrupt the plasma membrane integrity of A. flavus via leakage of intracellular electrolytes. Scanning electron microscopy indicated that the mycelial morphology of A. flavus was remarkably affected by (E)-2-heptenal. Metabolomic analyses indicated that 49 metabolites were significantly differentially expressed in A. flavus mycelia exposed to 0.2 µL/mL (E)-2-heptenal; these metabolites were mainly involved in galactose metabolism, starch and sucrose metabolism, the phosphotransferase system, and ATP-binding cassette transporters. ATP production was reduced in (E)-2-heptenal-treated A. flavus, and Janus Green B staining showed reduced cytochrome c oxidase activity. (E)-2-Heptenal treatment induced oxidative stress in A. flavus mycelia with an accumulation of superoxide anions and hydrogen peroxide and increased activities of superoxide dismutase and catalase. Simulated storage experiments showed that fumigation with 400 µL/L of (E)-2-heptenal vapor could completely inhibit A. flavus growth in wheat grains with 20% moisture; this demonstrates its potential use in preventing grain spoilage. This study provides valuable insights into understanding the antifungal effects of (E)-2-heptenal on A. flavus. KEY POINTS : ⢠(E)-2-Heptenal vapor showed the highest antifungal activity against A. flavus among (C5-C10) (E)-2-alkenals. ⢠The antifungal effects of (E)-2-heptenal against A. flavus were determined. ⢠The antifungal actions of (E)-2-heptenal on A. flavus were revealed by metabolomics and biochemical analyses.
Assuntos
Antifúngicos , Aspergillus flavus , Antifúngicos/farmacologia , Antifúngicos/metabolismo , Aldeídos/metabolismo , MetabolômicaRESUMO
Fungal spoilage of postharvest grains poses serious problems with respect to food safety, human health, and the economic value of grains. The protection of cereal grains from deleterious fungi is a critical aim in postharvest grain management. Considering the bulk volume of grain piles in warehouses or bins and food safety, fumigation with natural gaseous fungicides is a promising strategy to control fungal contamination on postharvest grains. Increasing research has focused on the antifungal properties of biogenic volatiles. This review summarizes the literature related to the effects of biogenic volatiles from microbes and plants on spoilage fungi on postharvest grains and highlights the underlying antifungal mechanisms. Key areas for additional research on fumigation with biogenic volatiles in postharvest grains are noted. The research described in this review supports the protective effects of biogenic volatiles against grain spoilage by fungi, providing a basis for their expanded application in the management of postharvest grains.
Assuntos
Fungos , Fungicidas Industriais , Humanos , Antifúngicos/farmacologia , Fungicidas Industriais/farmacologia , Grão Comestível/microbiologiaRESUMO
Acid modification has been widely used to modify the structural properties of biochars. However, acid modification led to the large consumption of acid, increased difficulty of waste effluent disposal, and a high application cost. To evaluate the advantages and application potential of biochars prepared under CO2, utilizing pyrolysis to directly modify biochars to improve heavy metal removal efficiency and reduce production cost, would be an important prerequisite for the broad application of biochars. The sorption performance of Pb2+ with CO2-modified biochars was compared with that of HNO3-modified biochar. The elemental compositions and structural properties of biochars were characterized through elemental analysis, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy. The results revealed that for biochars produced at 500â, HNO3 modification produced abundant carboxylic groups and -NO2 (asy) and -NO2 (sym) groups, promoting the surface activities and complexing abilities of biochars. The CO2-modified biochars contained abundant carbonate minerals, which could remove Pb2+ by electrostatic ion exchange and coprecipitation or complex. In addition, compared to that of HNO3-modified biochars, CO2-modified biochars had the larger specific surface area and better microporous structures, which were beneficial to the diffusion of Pb2+ and further promoted surface sorption. CO2 modification increased the maximum Pb2+ sorption capacity of W500CO2 and W700CO2, which were 60.14 mg·g-1 and 71.69 mg·g-1. By contrast, HNO3-modified biochars W500N2-A and W700N2-A showed the lower Pb2+ sorption capacities, which were 42.26 mg·g-1 and 68.3 mg·g-1, respectively. The increasing of the specific surface area and functional groups simultaneously promoted the sorption capacity of CO2-modified biochars. Consequently, the CO2-modified biochar had the advantages of low cost, environmental friendliness, and high heavy metal removal efficiency, which is a modification method worthy of promotion and application.
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Plant volatile organic compounds (VOCs) with antimicrobial activity could potentially be extremely useful fumigants to prevent and control the fungal decay of agricultural products postharvest. In this study, antifungal effects of volatile compounds in essential oils extracted from Origanum vulgare L. against Aspergillus flavus growth were investigated using transcriptomic and biochemical analyses. Carvacrol was identified as the major volatile constituent of the Origanum vulgare L. essential oil, accounting for 66.01 % of the total content. The minimum inhibitory concentrations of carvacrol were 0.071 and 0.18 µL/mL in gas-phase fumigation and liquid contact, respectively. Fumigation with 0.60 µL/mL of carvacrol could completely inhibit A. flavus proliferation in wheat grains with 20 % moisture, showing its potential as a biofumigant. Scanning electron microscopy revealed that carvacrol treatment caused morphological deformation of A. flavus mycelia, and the resulting increased electrolyte leakage indicates damage to the plasma membrane. Confocal laser scanning microscopy confirmed that the carvacrol treatment caused a decrease in mitochondrial membrane potential, reactive oxygen species accumulation, and DNA damage. Transcriptome analysis revealed that differentially expressed genes were mainly associated with fatty acid degradation, autophagy, peroxisomes, the tricarboxylic acid cycle, oxidative phosphorylation, and DNA replication in A. flavus mycelia exposed to carvacrol. Biochemical analyses of hydrogen peroxide and superoxide anion content, and catalase, superoxide dismutase, and glutathione S-transferase activities showed that carvacrol induced oxidative stress in A. flavus, which agreed with the transcriptome results. In summary, this study provides an experimental basis for the use of carvacrol as a promising biofumigant for the prevention of A. flavus contamination during postharvest grain storage.
Assuntos
Óleos Voláteis , Origanum , Antifúngicos/farmacologia , Antifúngicos/química , Óleos Voláteis/farmacologia , Óleos Voláteis/química , Aspergillus flavus , Origanum/química , Triticum , Monoterpenos/químicaRESUMO
Biochar has high carbon stability and is a good carbon sequestration material. Sludge biochar is rich in inorganic minerals, which would provide enrichment in the preparation process of pyrolysis, affecting its carbon sequestration capacity in practice. In this study, municipal sludge biochar (SZB), pharmaceutical sludge biochar (YCB), and chicken manure biochar (JFB) were prepared under the pyrolysis process at 500, 600, and 700â, respectively, and their aging process in soil for 70-100 years was simulated. The physicochemical properties and the carbon loss calculation of the biochars were determined using elemental analysis, FTIR, XRF, ICP, and XRD. The results demonstrated that the type and mass fraction of endogenous minerals in the biochars determined their carbon loss during pyrolysis. Ca and Mg were the main carbon-protecting minerals, whereas Fe may have reduced the carbon stability of the sludge biochars and therefore increased the carbon loss. For the aging process, the stability of the endogenous carbon in the biochars played a major role in its carbon loss, whereas the endogenous minerals played a supporting role. These findings elucidated the effect of the stability of endogenous carbon and the composition of mineral components on the carbon loss of biochars, which may provide references for soil carbon sequestration using sludge and chicken manure biochar.
Assuntos
Carbono , Gado , Animais , Esterco , Esgotos , Galinhas , SoloRESUMO
Migration characteristics of the heavy metals Fe, Zn, Mn, and Ni during the preparation of biochar from municipal sludge were studied, and the optimal pyrolysis temperature for the preparation of biochar was determined based on potential environmental risks. Four heavy metals (Fe, Zn, Mn, and Ni) with high total contents in the biochar were selected to determine their species and content changes under different pyrolysis temperatures using the BCR extraction method. An environmental risk assessment for sludge-based biochar was also carried out using the potential ecological risk index (PERI) and risk assessment code (RAC). The results showed that the volatility of the four metals is ranked as follows:Zn>Mn>Fe>Ni. The distribution and transformation of the four metal species were different, but their migration paths shared similar characteristics. In the pyrolysis stage at low temperatures (<500â), unstable fractions gradually changed into more stable species; under high temperatures (>500â), some of the oxidizable and residual fractions were broken, which transformed into reducible fractions, and other fractions escaped into the atmosphere. In the environmental risk assessment, biochar prepared under high pyrolysis temperatures (>500â) showed lower environmental risks, with the best outcomes at 500â.