RESUMO
As the rotation speed of ground based hyperspectral imaging system is too fast in the image collection process, which exceeds the speed limitation, there is data missed in the rectified image, it shows as the_black lines. At the same time, there is serious distortion in the collected raw images, which effects the feature information classification and identification. To solve these problems, in this paper, we introduce the each component of the ground based hyperspectral imaging system at first, and give the general process of data collection. The rotation speed is controlled in data collection process, according to the image cover area of each frame and the image collection speed of the ground based hyperspectral imaging system, And then the spatial orientation model is deduced in detail combining with the star scanning angle, stop scanning angle and the minimum distance between the sensor and the scanned object etc. The oriented image is divided into grids and resampled with new spectral. The general flow of distortion image corrected is presented in this paper. Since the image spatial resolution is different between the adjacent frames, and in order to keep the highest image resolution of corrected image, the minimum ground sampling distance is employed as the grid unit to divide the geo-referenced image. Taking the spectral distortion into account caused by direct sampling method when the new uniform grids and the old uneven grids are superimposed to take the pixel value, the precise spectral sampling method based on the position distribution is proposed. The distortion image collected in Lao Si Cheng ruin which is in the Zhang Jiajie town Hunan province is corrected through the algorithm proposed on above. The features keep the original geometric characteristics. It verifies the validity of the algorithm. And we extract the spectral of different features to compute the correlation coefficient. The results show that the improved spectral sampling method is better than the direct sampling method. It provides the reference for the similar product used on the ground.
RESUMO
As one of the most promising candidates for all-solid-state sodium-ion batteries and sodium-metal batteries, polyvinylidene difluoride (PVDF) and amorphous hexafluoropropylene (HFP) copolymerized polymer solid electrolytes still suffer from a relatively low room temperature ionic conductivity. To modify the properties of PVDF-HEP copolymer electrolytes, we introduce the graphitic C3N4 (g-C3N4) nanosheets as a novel nanofiller to form g-C3N4 composite solid polymer electrolytes (CSPEs). The analysis shows that the g-C3N4 filler can not only modify the structure in g-C3N4CSPEs by reducing the crystallinity, compared to the PVDF-HFP solid polymer electrolytes (SPEs), but also promote a further dissociation with the sodium salt through interaction between the surface atoms of the g-C3N4 and the sodium salt. As a result, enhanced electrical properties such as ionic conductivity, Na+ transference number, mechanical properties and thermal stability of the composite electrolyte can be observed. In particular, a low Na deposition/dissolution overpotential of about 100 mV at a current density of 1 mA cm-2 was found after 160 cycles with the incorporation of g-C3N4. By applying the g-C3N4 CSPEs in the sodium-metal battery with Na3V2(PO4)3 cathode, the coin cell battery exhibits a lower polarization voltage at 90 mV, and a stable reversible capacity of 93 mAh g-1 after 200 cycles at 1 C.
RESUMO
One of the most critical challenges for commercialization of sodium-ion battery (SIB) is to develop carbon anodes with high capacity and good rate performance. Graphene would be an excellent SIB anode candidate due to its success in various kinds of batteries. Liquid-phase exfoliation (LPE) method is an inexpensive, facile and potentially scalable method to produce less-defected graphene sheets. In this work, we developed an improved, dispersant-assisted LPE method to produce graphene composite materials from raw graphite with high yield and better quality for SIB anode. Here, bacterial cellulose (BC) was used as a green dispersant/stabilizer for LPE, a "spacer" for anti-restacking, as well as a carbon precursor in the composite. As a result, the carbonized BC (CBC)/LPE graphene (LEGr) presented improved performance compared to composite with graphene prepared by Hummers method. It exhibited a specific capacity of 233 mAh g-1 at a current density of 20 mA g-1, and 157 mAh g-1 after 200 cycles at a high current density of 100 mA g-1 with capacity retention rate of 87.73%. This method not only provides new insight in graphene composites preparation, but also takes a new step in the exploration of anode materials for sodium-ion batteriesSIBs.
RESUMO
Linear-array photoacoustic computed tomography (LA-PACT), for its flexibility and simplicity, has great potential in providing anatomical and functional information of tissues. However, the limited coverage view impedes the LA-PACT obtaining high-quality images. In this study, a photoacoustic tomographic system with a hyperbolic-array transducer was developed for stereoscopic PA imaging of carotid artery. The hyperbolic-array PACT increases the receiving sensitivity for PA signal detection due to its transducer's geometric structure matching with the spherical wave. The control phantom experiment shows that the proposed system can expand the angular coverage of â¼1/3 more than that of the LA-PACT system, and the volumetric PA images of rat's carotid artery demonstrates the potential of the system for carotid artery imaging. Furthermore, volumetric imaging of the human forearm verifies that the system has significant capability in human imaging, which indicates that it has bright prospect for assisting diagnosis in the vascular disease.
Assuntos
Técnicas Fotoacústicas , Animais , Imagens de Fantasmas , Ratos , Análise Espectral , Tomografia , Tomografia Computadorizada por Raios X , TransdutoresRESUMO
Imaging systems that integrate multiple modalities can reveal complementary anatomic and functional information as they exploit different contrast mechanisms, which have shown great application potential and advantages in preclinical studies. A portable and easy-to-use imaging probe will be more conducive to transfer to clinical practice. Here, we present a tri-modal ultrasonic (US), photoacoustic (PA), and thermoacoustic (TA) imaging system with an excitation-reception collinear probe. The acoustic field, light field, and electric field of the probe were designed to be coaxial, realizing homogeneous illumination and high-sensitivity detection at the same detection position. US images can provide detailed information about structures, PA images can delineate the morphology of blood vessels in tissues, and TA images can reveal dielectric properties of the tissues. Moreover, phantoms and in vivo human finger experiments were performed by the tri-modal imaging system to demonstrate its performance. The results show that the tri-modal imaging system with the proposed probe has the ability to detect small breast tumors with a radius of only 2.5 mm and visualize the anatomical structure of the finger in three dimensions. Our work confirms that the tri-modal imaging system equipped with a collinear probe can be applied to a variety of different scenarios, which lays a solid foundation for the application of the tri-modality system in clinical trials.
Assuntos
Neoplasias da Mama , Técnicas Fotoacústicas , Acústica , Feminino , Humanos , Imagens de Fantasmas , UltrassomRESUMO
We report a study in Drosophila melanogaster of latitudinal clines for 23 SNPs embedded in 13 genes (Pgi, Gapdh1, UGPase, Pglym78, Pglym87, Eno, Men, Gdh, Sod, Pgk, Mdh1, TreS, Treh) representing various metabolic enzymes. Our samples are from 10 populations spanning latitude from southern Florida to northern Vermont. Three new clines with latitude were detected. These are the amino acid polymorphisms in the NAD-dependent glutamate dehydrogenase (Gdh) and trehalase (Treh) genes, and a silent site polymorphism in the UDP-glucose pyrophosphorylase gene (UGPase). The result, when combined with the overall incidence and pattern of reports for six other genes (Adh, Gpdh, Pgm, G6pd, 6Pgd, Hex-C), presents a picture of latitudinal clines in metabolic genes prevalent around the branch point of competing pathways. For six of the seven amino acid polymorphisms showing significant latitudinal clines in North America, the derived allele is the one increasing with latitude, suggesting temperate adaptation. This is consistent with a model of an Afrotropical ancestral species adapting to temperate climates through selection favoring new mutations.
Assuntos
Adaptação Fisiológica/genética , Drosophila melanogaster/genética , Proteínas de Insetos/genética , Polimorfismo Genético , Alelos , Substituição de Aminoácidos , Animais , Drosophila melanogaster/metabolismo , Feminino , Masculino , Mutação , América do Norte , Seleção Genética , TemperaturaRESUMO
The allele frequency of four allozyme loci in four grasshopper species from two families of Catantopidae and Oedipodidae was examined using horizontal starch gel electrophoresis. The zymograms show that all four species have two loci in malate dehydrogenase (MDH). At MDH-1 one moderately migrated allele is shared and dominant in all four species. Locusta migratoria manilensi has two allele fixations in lactate dehydrogenase (LDH) and malic enzyme (ME); while Gastrimargus saussure has a fixed allele at MDH-1 and a unique fixation at MDH-2. The overall genetic variance was the highest in Oxya chinensis (Allele per locus = 3.0; He = 0.22), but the lowest in L. migratoria manilens (Allele per locus = 1.5; He = 0.013). Allozyme data suggests the four species are close in phylogenetic relationship, but differentiated in genetic variation levels.
Assuntos
Frequência do Gene , Variação Genética , Gafanhotos/genética , L-Lactato Desidrogenase/genética , Malato Desidrogenase/genética , Alelos , Animais , Genes Dominantes , Genética Populacional , Gafanhotos/enzimologia , Filogenia , Especificidade da EspécieRESUMO
The genetic structure of the two populations of Pararcyptera microptera meridionalis (Ikonn.) from Hebei and Liaoning in China was analyzed with horizontal starch gel electrophoresis. Among 15 loci of 11 enzymes identified in zymograms, Adk-1, Fbp-1, Mdh-2 and G3pd-1 showed low variability with few alleles. Higher allelic polymorphisms were observed at Fbp-2, Mdh-1 and Me-1. The two populations demonstrated high percentage of polymorphic loci (93.3% and 100.0%) but low observable overall heterozygosity (0.061 and 0.086), that could be attributed to heterozygote deficiencies, which led to the genotype frequency deviating from Hardy-Weinberg expectations. It is reasoned that the strong movement capability of the insect makes the individuals likely to be exposed to drastically varied environments, which tends to maintain dynamic equilibrium of genetic polymorphisms. The F-statistics between the two populations was comparatively smaller ( F(st) = 0.084), but larger when compared with those in migratory locusts like Locusta migratoria manilensis. Nei's genetic identity (I) and Roger's genetic distance (D) also showed close genetic relationship of the two populations by their high genetic identity (I = 0.904) and small genetic distance (D = 0.256). However,considerably qualitative and quantitative differences were noted at loci Acp-1 (F(st) = 0.462) and Pgi-1 (F(st) = 0.182).
Assuntos
Isoenzimas/genética , Ortópteros/enzimologia , Ortópteros/genética , Animais , Frequência do Gene , Variação Genética , Heterozigoto , Ortópteros/classificaçãoRESUMO
The rice grasshopper Oxya chinensis exhibits polymorphic loci at Ldh, Gpi, Pgm and Me. The data of the mean number of alleles per locus (A = 2.8), percentage of polymorphic loci (P = 80.0%), the observed mean heterozygosities (Ho = 0.271 approximately 0.279) and the expected mean heterozygosities (He = 0.305 approximately 0.316) of the species suggest that O. chinensis possesses sufficient genetic diversity. It was hypothesized that the high polymorphisms at Ldh, Gpi, Pgm and Me might make it possible for pesticide avermectin to act as a selective agent through differential lethality among the insect individuals with different genotypes. In this study a total of 855 grasshoppers were injected with avermectin (1.3 x 10(-2) g/g) to obtain a mortality of 54% after 24 hours. The allozyme analysis was then employed to determine the genotypes of Ldh, Gpi, Pgm and Me for both dead and surviving individuals. Contingency table chi2 tests showed that avermectin displayed random lethal effects on the genotypes at the loci of Ldh, Pgm and Me, without correlation between the genotype and mortality. In contrast, at Gpi locus, the grasshopper demonstrated a mortality cline of Gpi-AA (38%), Gpi-AB (51%), Gpi-BB (58%) and Gpi-BC (74%). The significant mortality differences were found among the following genotype pairs: Gpi-AA vs. Gpi-BB, Gpi-AA vs. Gpi-BC and Gpi-AB vs. Gpi-BC. These data implied the Gpi-AA genotype was likely related to the specie's resistance to the pesticide avermectin. It was also noted that the Gpi-A allele was present in the genotypes with low morality,while Gpi-B was present in the genotypes with moderate mortality, and the individuals with Gpi-C allele exhibited the highest mortality. The data obtained in this study suggested that the increasing proportion of Gpi-AA genotype and perhaps Gpi-A allele in a population may be useful as a potential resistant biomarker of O. chinensis to pesticide avermectin.
Assuntos
Gafanhotos/enzimologia , Gafanhotos/genética , Inseticidas/farmacologia , Ivermectina/análogos & derivados , Ivermectina/farmacologia , Alelos , Animais , Genótipo , Glucose-6-Fosfato Isomerase/genética , L-Lactato Desidrogenase/genética , Fosfoglucomutase/genéticaRESUMO
The allozyme analysis using horizontal starch gel electrophoresis was employed to compare the genetic structure in the population of oriental migratory locust Locusta migratoria manilensis from two breeding areas, Beidagang(Tianjin) and Huanghua(Hebei). The two areas are adjacent but with distinct ecological features, with the recorded locust outbreaks and migration. The zymograms showed that among nineteen loci four (Mdh-1, Pgm, Adk and G3pd) showed extremely low variability level with the frequency of the most common allele higher than 0.95 in the populations from both sites. The rest loci had 2 to 4 alleles but the allele frequencies between the two populations were all similar except Fbp and Got-2 loci. In the 27 chi 2-tests for the genotypes at polymorphic loci only two (Pgi and Got-1) of beidagang population did fit the Hardy-Weinberg's expectations. This is due to high frequencies of the most common homozygotes and the corresponding heterozygote deficiency. The allozyme data demonstrated that the locusts had remarkable genetic variability within each population, but little divergence between the populations. The genetic variability measurements were found similar: Percentage of polymorphic loci (P) was between 73.7% and 78.9%; The mean number of alleles per locus (A) was from 2.9 to 3.1; and the mean heterozygosity (Ho) was nearly identical (about 0.138). The F-statistics (FST = 0.053) also showed the genetic uniformity of the populations, corresponding to the high Nei's genetic identity (I = 0.938). These results of the allozyme analysis suggested that the two populations appeared to be a part of a large population. It is reasoned that the genetic polymorphism and differentiation at certain loci between the two populations may depend on at least two agnostic factors that are all related to migration. First, the unusual dispersal capability of L. m. manilensis tends to make a continuous genetic structure distribution. Second, the frequent migration also results in the individuals to be exposed to drastically various environments. Since the broad adaptability is crucial to survive the changing environments, the genetic variation at population level is necessarily required to offer the population resilience for successful survival and reproduction under those ecologically divergent abiotic and biotic conditions. Thus, the migration contributes to the maintenance of dynamic equilibrium of genetic polymorphism in this highly specialized subspecies.
Assuntos
Enzimas/genética , Gafanhotos/enzimologia , Alelos , Animais , China , DNA/genética , Eletroforese em Gel de Amido , Enzimas/análise , Frequência do Gene , Genética Populacional , Genótipo , Gafanhotos/genética , Desequilíbrio de LigaçãoRESUMO
The genetic structure of eight locust species in three families (Catantopidae, Oedipodidae and Arcypteridae) from Shanxi Province in China was compared using allozyme analysis with horizontal starch gel electrophoresis. Among 17 loci identified in zymograms, Ao-1, Est-3, G3pd-1, Idh-2 and Mdh-2 had low variability with a few alleles. High polymorphism was observed at Ldh-1, Me-1 and Gpi-1. Each of the eight species demonstrated high percentage of polymorphic loci (P = 64.7%-94.1%) but low observed heterozygosity (H0 = 0.024-0.087) due to heterozygote deficiency. It was noted that the migratory locusts usually had higher percentage of polymorphic loci (P = 88.2%-94.1%) than non-migratory species (P = 64.7%-94.1%). The only exception is Oxya chinensis(P = 94.1%). It is reasoned that the higher polymorphism is necessary for migratory species to cope with the environments that might be drastically different from the habitats before migration. The taxon relationships using cluster analysis based on Nei's genetic identity (I) and Roger's genetic distance (D) were the same at species and genus levels. The differences were found at family level, possibly due to the alternative algorithms. The cladogram using Roger's genetic distance (D) overlapped the relationship obtained from karyotypic analyses, which demonstrated that the species examined in Catantopidae displayed somewhat closer relationship to those in Oedipodidae than to those in Arcypteridae. It is suggested that the allozyme analysis is useful as molecular marker for locusts in phylogenetic reconstruction at the species and genus level, while additional data from other studies are necessary when used for higher taxa.
Assuntos
Enzimas/genética , Gafanhotos/genética , Alelos , Animais , China , Eletroforese em Gel de Amido , Enzimas/análise , Frequência do Gene , Genética Populacional , Genótipo , Gafanhotos/classificação , Gafanhotos/enzimologia , Desequilíbrio de Ligação , FilogeniaRESUMO
Allozyme electrophoresis was employed to compare the difference in mortality among the genotypes at two polymorphic loci of Pgm and Me of grasshopper Oxya chinensis individuals acutely exposed to 1.5g/L malathion which resulted in 56% mortality in 24 hours. The selective lethal effects were observed among the genotypes at Pgm locus but not at Me locus. It is noted that the genotype Pgm-ab experienced the highest mortality (80%), whereas Pgm-bb and Pgm-bc were 49%, lower than the average. The chi(2) tests showed significant difference in morality between Pgm-bb and Pgm-cc. After exposure the allele frequency of Pgm-b showed a notable increase among surviving individuals. The cluster analysis based on Roger's genetic distance indicated that the acute exposure to malathion can cause differentiation in genetic composition at population level in Oxya chinensis. Because malathion is commonly used as the insecticide for grasshopper control, the data obtained in this study suggest that the similar genotype-mortality effects may occur in crop fields.
Assuntos
Gafanhotos/efeitos dos fármacos , Inseticidas/farmacologia , Malation/farmacologia , Fosfoglucomutase/genética , Polimorfismo Genético/efeitos dos fármacos , Alelos , Animais , Análise por Conglomerados , Frequência do Gene , Variação Genética , Genética Populacional , Genótipo , Gafanhotos/classificação , Gafanhotos/enzimologia , Gafanhotos/genética , Malato Desidrogenase/efeitos dos fármacos , Malato Desidrogenase/genética , Fosfoglucomutase/efeitos dos fármacosRESUMO
Using the plant survivorship theory, the age structure, and the relationship between tree height and diameter (DBH) of Quercus wutaishanica population in Lingkong Mountain were analyzed, and the static life table was compiled and the survival curve plotted. The shuttle shape in age structure of Q. wutaishanica population suggested its temporal stability. The linear regression significantly fitted the positive correlation between tree height and DBH. The maximal life expectancy was observed among the trees beyond the age of the highest mortality and coincided with the lowest point of mortality density, suggesting the strong vitality of the seedlings and young trees that survived in the natural selection and intraspecific competition. The population stability of the Q. wutaishanica population was characterized by the Deevey-II of the survival curve. The dynamic pattern was characterized by the recession in the early phase, growth in the intermediate phase, and stability in the latter phase.
Assuntos
Quercus/crescimento & desenvolvimento , China , Ecossistema , Dinâmica Populacional , Plântula , ÁrvoresRESUMO
The phylogenetic relationships of genera in the subfamily Apaturinae were examined using mtDNA sequence data from 1,471 bp of cytochrome oxidase subunit?(COI). The mitochondrial COI gene from a total of 16 species in 11 genera were sequenced to obtain mtDNA data, along with those of 4 species obtained from GenBank, to construct the MP and the NJ trees using Athyma jina, Penthema adelma, Polyura nepenthes, and Charaxes bernardus as outgroups. The transitions at the third codon positions of the COI data set were found saturated, but they were retained for analysis, because they contain the majority of the phylogenetic information. The impacts of equal weight assumptions for all characters in the parsimonious analysis were assessed by potential alternations in clades in response to different transition/transversion weighting schemes. The results indicated four distinct major groups in Apaturinae. Moreover, several well supported and stable clades were found in the Apaturinae. The study also identified undetermined taxon groups whose positions were weakly supported and were subject to changes under different weighting schemes. Within the Apaturinae, the clustering results are approximately identical to the classical morphological classification. The mtDNA data suggest the genus Mimathyma as a monophyletic group. Lelecella limenitoides and Dilipa fenestra have close relationship with very strong support in all phylogenetic trees. It also supports the taxonomic revision of removing several species from Apatura to other genera, namely Mimathyma schrenckii, M. chevana, M. nycteis, Chitoria subcaerulea, C. fasciola, C. pallas, and Helcyra subalba.
Assuntos
Complexo IV da Cadeia de Transporte de Elétrons/genética , Genes Mitocondriais , Lepidópteros/classificação , Lepidópteros/genética , Filogenia , Animais , Sequência de Bases , DNA Mitocondrial/genética , Lepidópteros/citologia , Lepidópteros/enzimologia , MasculinoRESUMO
Allozyme analysis, microsatellite primer PCR (SSRP-PCR), and amplified fragment length polymorphism (AFLP) techniques were used to assess genetic diversity and population structure of the Chinese oriental migratory locust, Locusta migratoria manilensis. A total of 299 PCR markers (67 SSRPs and 232 AFLPs) were detected in eight populations, of which 98.7% were polymorphic markers. The proportion of polymorphic loci (95.5-98.8%) by SSRP+AFLP markers indicated no significant differences between populations, and all populations exhibited a similar level of variability; results of the allozyme analysis demonstrated that 19 loci gave rise to a lower level of polymorphism (55.6-66.7%). The genetic distances between the populations were relatively low. Shannon's index and Nei's gene diversity showed low differentiation among the populations. Allozyme analysis, however, reflected greater similarity and smaller differentiation between the populations than those shown by SSRP and AFLP markers. Neighbor-joining dendrograms derived from both the allozyme and SSRP+AFLP markers showed that the genetic distances among Chinese oriental migratory locust populations were not greatly influenced by geographic distance and breeding habitats.