RESUMO
DNA polymerase ß (Pol ß) repairs cellular DNA damage. When such damage is inflicted upon the DNA in tumor cells treated with DNA targeted antitumor agents, Pol ß thus diminishes their efficacy. Accordingly, this enzyme has long been a target for antitumor therapy. Although numerous inhibitors of the lyase activity of the enzyme have been reported, none has yet proven adequate for development as a therapeutic agent. In the present study, we developed a new strategy to identify lyase inhibitors that critically engage the lyase active site primary nucleophile Lys72 as part of the binding interface. This involves a parallel evaluation of the effect of the inhibitors on the wild-type DNA polymerase ß (Pol ß) and Pol ß modified with a lysine analogue at position 72. A model panel of five structurally diverse lyase inhibitors identified in our previous studies (only one of which has been published) with unknown modes of binding were used for testing, and one compound, cis-9,10-epoxyoctadecanoic acid, was found to have the desired characteristics. This finding was further corroborated by in silico docking, demonstrating that the predominant mode of binding of the inhibitor involves an important electrostatic interaction between the oxygen atom of the epoxy group and Nε of the main catalytic nucleophile, Lys72. The strategy, which is designed to identify compounds that engage certain structural elements of the target enzyme, could find broader application for identification of ligands with predetermined sites of binding.
Assuntos
DNA Polimerase beta/metabolismo , Ácidos Esteáricos/metabolismo , Sítios de Ligação , Domínio Catalítico , DNA Polimerase beta/antagonistas & inibidores , DNA Polimerase beta/genética , Humanos , Ligantes , Simulação de Acoplamento Molecular , Mutagênese Sítio-Dirigida , Ligação Proteica , Ácidos Esteáricos/químicaRESUMO
[reaction: see text] 10,11-Methylenedioxy-14-azacamptothecin, a potent analogue of the antitumor agent camptothecin (CPT), has been prepared via a key condensation between AB and DE ring precursors. The biological testing of this compound validated a strategy for modulation of the off-rate of camptothecin analogues from the topoisomerase-DNA-CPT ternary complex via structural modification.
Assuntos
Antineoplásicos Fitogênicos , Camptotecina , Inibidores da Topoisomerase I , Antineoplásicos Fitogênicos/síntese química , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Camptotecina/análogos & derivados , Camptotecina/síntese química , Camptotecina/química , Camptotecina/farmacologia , DNA/química , DNA Topoisomerases Tipo I/genética , Ensaios de Seleção de Medicamentos Antitumorais , Estrutura Molecular , Relação Estrutura-AtividadeRESUMO
14-Azacamptothecin, a potent, water-soluble analogue of the antitumor agent camptothecin, has been prepared by a convergent synthesis. The key condensation of the AB and DE rings with concomitant formation of ring C of 14-aza CPT was carried out in two stages, the latter of which involved a radical cyclization strategy. [structure: see text]
Assuntos
Antineoplásicos/síntese química , Camptotecina/análogos & derivados , Inibidores da Topoisomerase I , Antineoplásicos/farmacologia , Camptotecina/síntese química , Camptotecina/farmacologia , Ciclização , Humanos , Estrutura Molecular , Água/químicaRESUMO
[structure: see text] In an effort to improve the water solubility of camptothecin, four 20-O-phosphate and phosphonate analogues have been prepared. These analogues are freely water soluble, stable at physiological pH, and stabilize the human topoisomerase I-DNA covalent binary complex with the same sequence selectivity as camptothecin itself. All four compounds inhibited the growth of yeast expressing human topoisomerase I in an enzyme-dependent fashion.
Assuntos
Camptotecina/análogos & derivados , Camptotecina/farmacologia , Inibidores da Topoisomerase I , Camptotecina/química , DNA/química , DNA/metabolismo , DNA Topoisomerases Tipo I/metabolismo , Humanos , Concentração de Íons de Hidrogênio , Estrutura Molecular , Saccharomyces cerevisiae , SolubilidadeRESUMO
A series of iminoxyl radicals of the general formula R(C=NO(*))R(1), with R and R(1) usually tertiary, was synthesized in a search for radicals of increased persistence. Three new radicals were isolated as blue liquids: Et(3)C(C=NO(*))Bu-t (1), t-C(5)H(11)(C=NO(*))Bu-t (2), and (t-C(5)H(11))(2)C=NO(*) (3). Oxidation of oximes Et(3)C(C=NOH)Ph (4H), PhCH(2)CMe(2)(C=NOH)Bu-t (5H), PhCMe(2)(C=NOH)Bu-t (6H), and Me(2)CH(C=NOH)C(5)H(11)-t (7H), among others, did not lead to isolable iminoxyls. A new, convenient synthesis of symmetrical tertiary imines from tert-RCl, tert-RCN, and Na is described. Radical t-Bu(2)C=NO(*) (8) and cyclohexene readily gave the allylic substitution product, 2,2,4,4-tetramethyl-3-hexanone O-(2'-cyclohexen-1'-yl)oxime.
RESUMO
To investigate how structural changes in the amino acid side chain affect nucleotide substrate selection in human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT), a variety of non-natural tyrosine analogues were substituted for Tyr115 of p66 RT. RT variants containing meta-Tyr, nor-Tyr, aminomethyl-Phe, and 1- and 2-naphthyl-Tyr were produced in an Escherichia coli coupled transcription/translation system. Mutant p66 subunits were reconstituted with wild-type (WT) p51 RT and purified by affinity chromatography. Each modified enzyme retained DNA polymerase activity following this procedure. Aminomethyl-Phe115 RT incorporated dCTP more efficiently than the WT and was resistant to the chain terminator (-)-beta-2',3'-dideoxy-3'-thiacytidine triphosphate (3TCTP) when examined in a steady-state fidelity assay. However, 2-naphthyl-Tyr115 RT inefficiently incorporated dCTP at low concentrations and was kinetically slower with all dCTP analogues tested. Models of RT containing these side chains suggest that the aminomethyl-Phe115 substitution provides new hydrogen bonds through the minor groove to the incoming dNTP and the template residue of the terminal base pair. These hydrogen bonds likely contribute to the increased efficiency of dCTP incorporation. In contrast, models of HIV-1 RT containing 2-naphthyl-Tyr115 reveal significant steric clashes with Pro157 of the p66 palm subdomain, necessitating rearrangement of the active site.
Assuntos
Transcriptase Reversa do HIV/química , Transcriptase Reversa do HIV/metabolismo , HIV-1/enzimologia , Engenharia de Proteínas , Substituição de Aminoácidos , Sítios de Ligação , Sistema Livre de Células , Citidina Trifosfato/análogos & derivados , Citidina Trifosfato/metabolismo , Citidina Trifosfato/farmacologia , DNA/biossíntese , DNA/efeitos dos fármacos , Nucleotídeos de Desoxicitosina/metabolismo , Didesoxinucleotídeos , Escherichia coli , Ligação de Hidrogênio , Cinética , Lamivudina/análogos & derivados , Lamivudina/metabolismo , Lamivudina/farmacologia , Modelos Moleculares , Biossíntese de Proteínas , Inibidores da Transcriptase Reversa/metabolismo , Inibidores da Transcriptase Reversa/farmacologia , Transcrição Gênica , Tirosina/análogos & derivadosRESUMO
A recent X-ray crystallographic analysis of the binding of a water soluble camptothecin analogue to the human topoisomerase I-DNA covalent binary complex has suggested the existence of some novel features in the way that camptothecin is bound to the binary complex. Four additional models based on chemical and biochemical data have also been proposed. Presently we describe S-containing analogues of camptothecin prepared on the basis of these models, and report their ability to form stable ternary complexes with human topoisomerase I, and to mediate cytotoxicity at the locus of topoisomerase I. The results indicate that replacement of the 20-OH group of CPT with a SH functionality results in diminution of the potency of CPT as a topoisomerase I poison, while replacement of the O atoms at positions 20 and 21 with S atoms results in essentially complete loss of topoisomerase I inhibitory activity.
Assuntos
Camptotecina/metabolismo , DNA Topoisomerases Tipo I/metabolismo , Inibidores Enzimáticos/metabolismo , Oxigênio/química , Autorradiografia , Cristalografia por Raios X , Humanos , Espectroscopia de Ressonância Magnética , Espectrometria de Massas por Ionização por ElectrosprayRESUMO
On the basis of an analysis of luotonin A and its D-ring deaza analogue as topoisomerase I poisons and topoisomerase I-dependent cytotoxic agents, a novel analogue of the structurally related antitumor antibiotic camptothecin (CPT) was prepared. 14-Azacamptothecin was found to have much greater aqueous solubility than CPT, to inhibit topoisomerase I-mediated DNA relaxation more efficiently than CPT, and to stabilize the covalent binary complex to almost the same extent. 14-Aza CPT was found to be slightly less active than CPT in mediating cytotoxicity toward yeast expressing human topoisomerase I, possibly as a consequence of its greater off-rate from the CPT-topoisomerase I-DNA ternary complex.
Assuntos
Camptotecina/análogos & derivados , Camptotecina/química , Camptotecina/farmacologia , Inibidores Enzimáticos/química , Inibidores Enzimáticos/farmacologia , Inibidores da Topoisomerase I , Humanos , Cinética , Pirróis/química , Pirróis/farmacologia , Quinonas/química , Quinonas/farmacologia , Solubilidade , Água/químicaRESUMO
By employing a general biosynthetic method for the elaboration of proteins containing unnatural amino acid analogues, we incorporated (aminooxy)acetic acid into positions 10 and 27 of Escherichia coli dihydrofolate reductase. Introduction of the modified amino acid into DHFR was accomplished in an in vitro protein biosynthesizing system by readthrough of a nonsense (UAG) codon with a suppressor tRNA that had been activated with (aminooxy)acetic acid. Incorporation of the amino acid proceeded with reasonable efficiency at codon position 10 but less well at position 27. (Aminooxy)acetic acid was also incorporated into position 72 of DNA polymerase beta. Peptides containing (aminooxy)acetic acid have been shown to adopt a preferred conformation involving an eight-membered ring that resembles a gamma-turn. Accordingly, the present study may facilitate the elaboration of proteins containing conformationally biased peptidomimetic motifs at predetermined sites. The present results further extend the examples of ribosomally mediated formation of peptide bond analogues of altered connectivity and provide a conformationally biased linkage at a predetermined site. It has also been shown that the elaborated protein can be cleaved chemically at the site containing the modified amino acid.
Assuntos
Ácido Amino-Oxiacético/metabolismo , Proteínas/metabolismo , Aminoacil-RNA de Transferência/química , Tetra-Hidrofolato Desidrogenase/química , Sequência de Aminoácidos , Animais , Sítios de Ligação , Códon , DNA Polimerase beta/química , DNA Polimerase beta/metabolismo , Escherichia coli/enzimologia , Metionina/metabolismo , Modelos Moleculares , Dados de Sequência Molecular , Conformação de Ácido Nucleico , RNA Mensageiro/genética , Aminoacil-RNA de Transferência/metabolismo , Coelhos , Reticulócitos/metabolismo , Tetra-Hidrofolato Desidrogenase/genética , Tetra-Hidrofolato Desidrogenase/metabolismoRESUMO
Luotonin A is a pyrroloquinazolinoquinoline alkaloid isolated from the Chinese herbal medicinal plant Peganum nigellastrum. Although previously shown to exhibit cytotoxicity against the murine leukemia P-388 cell line, the mechanism of action of luotonin A is unknown. Presently, we demonstrate that luotonin A stabilizes the human DNA topoisomerase I-DNA covalent binary complex, affording the same pattern of cleavage as the structurally related topoisomerase I inhibitor camptothecin. Luotonin A also mediated topoisomerase I-dependent cytotoxicity toward Saccharyomyces cerevisiae lacking yeast topoisomerase I, but harboring a plasmid having the human topoisomerase I gene under the control of a galactose promoter. This finding identifies a putative biochemical locus for the cytotoxic action of luotonin A and has important implications for the mechanism of action of camptothecin and the design of camptothecin analogues.
Assuntos
Alcaloides/química , Alcaloides/farmacologia , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , DNA Topoisomerases Tipo I/metabolismo , Inibidores Enzimáticos/química , Inibidores Enzimáticos/farmacologia , Pirróis/química , Pirróis/farmacologia , Quinonas/química , Quinonas/farmacologia , Inibidores da Topoisomerase I , Camptotecina/farmacologia , DNA/química , DNA/metabolismo , HumanosRESUMO
Luotonin A is a cytotoxic pyrroloquinazolinoquinoline alkaloid that has been shown to stabilize the human topoisomerase I-DNA covalent binary complex in the same fashion as the antitumor alkaloid camptothecin. A study of the structural elements in luotonin A required for binary complex stabilization has revealed key differences relative to those required for camptothecin.
Assuntos
Pirróis/química , Quinonas/química , Inibidores Enzimáticos/química , Inibidores Enzimáticos/farmacologia , Humanos , Pirróis/farmacologia , Quinonas/farmacologia , Inibidores da Topoisomerase IRESUMO
Luotonin A, a naturally occurring pyrroloquinazolinoquinoline alkaloid, has been previously demonstrated to be a topoisomerase I poison. A number of luotonin A derivatives have now been prepared through the condensation of anthranilic acid derivatives and 1,2-dihydropyrrolo[3,4-b]quinoline-3-one in the presence of phosphorus oxychloride. When dichloromethane was used as solvent the reaction proceeded to a single product. In contrast when the reaction was carried out in tetrahydrofuran or in phosphorus oxychloride, an additional isomeric product was obtained. The luotonin A analogues were evaluated for their ability to effect stabilization of the covalent binary complex formed between human topoisomerase I and DNA, and for cytotoxicity toward a yeast strain expressing the human topoisomerase I.
Assuntos
Pirróis/síntese química , Quinonas/síntese química , Inibidores da Topoisomerase I , DNA/metabolismo , DNA Topoisomerases Tipo I/genética , DNA Topoisomerases Tipo I/metabolismo , Humanos , Organismos Geneticamente Modificados , Pirróis/farmacologia , Quinonas/farmacologia , Saccharomyces cerevisiae/efeitos dos fármacos , Saccharomyces cerevisiae/genética , Solventes , Relação Estrutura-AtividadeRESUMO
Methylphosphonate-bearing oligonucleotides are characterized by the replacement of one of the nonbridging oxygen atoms with a methyl group. While neutralizing the negative charge associated with the phosphodiester at the point of substitution, the methyl group also imparts chirality to the phosphorus atom. Herein we report the synthesis of a number of oligonucleotides containing isomerically pure S(p) and R(p) methylphosphonates at single positions for the purpose of investigating the hydrogen-bonding contacts necessary for human topoisomerase I function. It was possible to correlate these data to the recent X-ray crystal structure of a truncated form of the enzyme and demonstrate a severe decrease of cleavage efficiency when any of the nonbridging oxygen atoms upstream from the cleavage site was removed. Also observed was increased cleavage for oligonucleotides substituted with methylphosphonates downstream from the cleavage site. These effects were shown to be due primarily to alteration of the binding of the modified DNA substrates by human DNA topoisomerase I.
Assuntos
DNA Topoisomerases Tipo I/metabolismo , DNA/metabolismo , Compostos Organofosforados/química , Oxigênio/química , Pareamento de Bases , Sequência de Bases , DNA/química , DNA Topoisomerases Tipo I/química , Humanos , Ligação de Hidrogênio , Modelos Moleculares , Estrutura Molecular , Oligonucleotídeos/química , Oligonucleotídeos/metabolismoRESUMO
A highly efficient cell-free translation system has been combined with suppressor tRNA technology to substitute nor-Tyr and 3-fluoro-Tyr in place of Tyr183 at the DNA polymerase active site of p66 of human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT). Supplementing the wild-type HIV-1 p51 RT subunit into this translation system permitted reconstitution of the biologically relevant p66/p51 heterodimer harboring Tyr analogs exclusively on the catalytically competent p66 subunit. Addition of an affinity tag at the p66 C-terminus allowed rapid, one-step purification of reconstituted and selectively mutated heterodimer HIV-1 RT via strep-Tactin-agarose affinity chromatography. The purified enzyme was demonstrated to be free of contaminating nucleases, allowing characterization of the DNA polymerase and ribonuclease H activities associated with HIV-1 RT. Preliminary characterization of HIV-1 RT(nor-Tyr) and HIV-1 RT(m-fluoro-Tyr) is presented. The success of this strategy will facilitate detailed molecular analysis of structurally and catalytically critical amino acids via their replacement with closely related, unnatural analogs.