Hepatoprotective action of papain-hydrolyzed buffalo milk protein on carbon tetrachloride oxidative stressed albino rats.

Buffalo skim milk retentate was hydrolyzed with papain for 4 h (enzyme:substrate, 1:200), resulting in a retentate hydrolysate (RH) with a degree of hydrolysis of 23%. We then investigated the potential hepatoprotective activity of RH at 250 and 500 mg/kg of body weight per day on carbon tetrachloride (CCl4)-induced oxidative stress in albino rats. Liver biomarkers (alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase), kidney biomarkers (urea, creatinine), and serum lipid profile (total lipids and triglycerides) were measured, in addition to histopathological status. Injection of CCl4 significantly increased all liver and kidney biomarkers compared with the negative control. In contrast, CCl4 injection significantly reduced hepatic antioxidant enzyme activities; that is, glutathione peroxidase and superoxide dismutase. Oral administration of RH for 28 d effectively maintained a physiologically normal range of liver and kidney biomarkers compared with the positive control. Furthermore, RH administration significantly increased activities of glutathione peroxidase and superoxide dismutase. Histopathological sections of CCl4-stressed rats treated with RH were different from that of the positive control and were similar to those of the negative control, in a concentration-dependent manner. Our results demonstrated the antihepatotoxic activities of buffalo milk RH and demonstrated that the higher RH concentration (500 mg/kg of body weight per day) could maintain the healthy biological status of the CCl4-injected rats.

Comparative study of water sorption, solubility, and tensile bond strength of two soft lining materials.

STATEMENT OF PROBLEM: Two soft denture lining materials with distinct chemical compositions were evaluated to determine whether these compositional variations manifest themselves in property differences. PURPOSE: This study evaluated and compared the water sorption, solubility, and tensile bond strength of a recently introduced silicone-based soft liner (Luci-sof) and a plasticized acrylic resin soft liner (Permasoft) using 2 processing techniques, laboratory-processed and autopolymerized at chairside, for the latter. MATERIAL AND METHODS: For water sorption and solubility testing, 24 disks (45 mm in diameter and 1 mm in thickness) were prepared for each group, stored in distilled water at 37 degrees C, and tested after 1, 4, and 6 weeks. The disks used for the first week were again tested after 4 and 6 weeks of continuous storage. Their weight was recorded and sorption and solubility were calculated using 2 methods. Two PMMA cylinders (25 mm in diameter, 25 mm in length), joined together by the soft liner, were used to determine the bond strength in tension at a loading rate of 2 mm/min after 48 hours and 12 weeks aging in distilled water. RESULTS: Permasoft had higher solubility (1.50% +/- 0.20% laboratory-processed and 1.42% +/- 0.16% autopolymerized at chairside) and sorption (2.45% +/- 0.36% laboratory-processed and 1. 76% +/- 0.08% autopolymerized at chairside) than Luci-sof (0.17% +/- 0.09% solubility and 0.41% +/- 0.17% sorption) after 6 weeks of aging. For tensile bond strength, there was no significant difference between each respective control and the 12-week bond strength for each material. However, Luci-sof (1.16 +/- 0.27 MPa) had a significantly higher tensile bond strength than Permasoft (0. 50 +/- 0.18 MPa laboratory-processed and 0.44 +/- 0.09 MPa autopolymerized at chairside). CONCLUSION: On the basis of lower water sorption and solubility and higher tensile bond strength, Luci-sof may provide better clinical success.