Health risk assessment related to cyanotoxins exposure of a community living near Tri An Reservoir, Vietnam.

Cyanotoxins released by cyanobacteria are currently a concern due to potential impacts on plants, animals, and human health. Many instances of cyanotoxin poisoning have been reported around the world, including acute, chronic, and fatal cases. In recent years, the Tri An Reservoir (TAR) in Vietnam has experienced influxes of cyanotoxins from toxic blue-green algae at levels which exceed the World Health Organization's (WHO) permitted level of 1 µg/L. Previous studies have focused on assessing cyanobacterial diversity, the presence of cyanotoxins in water, or the effect on aquatic plants and animals. Therefore, this study aims to assess the health risks associated with cyanobacteria exposure for people living near the TAR in Dong Nai Province, Vietnam, using the Monte Carlo simulation technique. In total, 120 water samples were collected at five points of the reservoir from 2017 to 2019. Seventy-five local people who have been exposed to the water from the reservoir were interviewed. Microcystin (MC) concentration was measured with a high-pressure liquid chromatography (HPLC) system and was used to assess the health risk to local people. The results showed that the MC concentration in raw water ranged from below detection limit (BDL) to 18.67 µg/L in 2017 and from BDL to 8.6 µg/L in 2019, with the predominant variant being MC-RR. The concentration of MCs in the TAR in 2017 and 2019 exceeded the WHO's permitted level of 1 µg/L by 76% and 19%, respectively. The results showed that the rate of MC exposure likely to cause cancer was approximately 1-5%. The oral exposure rate while bathing was less than 2.5%. Direct oral exposure of cyanobacteria with no carcinogenicity was relatively high (40-50%) and the most affected group was adults.

Ultrasensitive DNA detection using oligonucleotide-silver nanoparticle conjugates.

Oligonucleotide-gold nanoparticle (OGN) conjugates are powerful tools for the detection of target DNA sequences due to the unique properties conferred upon the oligonucleotide by the nanoparticle. Practically all the research and applications of these conjugates have used gold nanoparticles to the exclusion of other noble metal nanoparticles. Here we report the synthesis of oligonucleotide-silver nanoparticle (OSN) conjugates and demonstrate their use in a sandwich assay format. The OSN conjugates have practically identical properties to their gold analogues and due to their vastly greater extinction coefficient both visual and absorption analyses can occur at much lower concentrations. This is the first report of OSN conjugates being successfully used for target DNA detection and offers improved sensitivity which is of interest to a range of scientists.

Stereoinversion of beta- and gamma-substituted alpha-amino acids using a chemo-enzymatic oxidation-reduction procedure.

Both D- and L-beta- and gamma-substituted alpha-amino acids can be interconverted to their respective L- and D- diastereoisomers by treatment with an enantioselective amino acid oxidase and a chemical reducing agent.

A new approach for DNA detection by SERRS.

A new approach for the detection of DNA using surface enhance resonance Raman scattering (SERRS) is reported. The majority of existing techniques use fluorescence spectroscopy with advanced probe design to provide information on the identity of specific DNA sequences down to single base resolution. A new approach to the labelling of DNA is discussed which uses Michael addition to couple thiolated DNA to dye labels specifically designed to attach to silver surfaces. When combined with existing strategies for sensitive detection of DNA using commercially available labels, a new class of biomolecular probe known as a SERRS Beacon was produced. The detection techniques of fluorescence and surface enhanced resonance Raman scattering (SERRS) are combined to give a sensitive and selective system for use in the development and creation of novel assays for specifically defined targets. It demonstrates improved potential for multiplexing analysis.

SERRS dyes. Part 3. Synthesis of reactive benzotriazole azo dyes for surface enhanced resonance Raman scattering.

A series of eleven specially designed benzotriazole monoazo dyes for use in surface enhanced resonance Raman scattering studies are reported. Unlike previous benzotriazole dyes produced for SERRS, these dyes have been synthesised to be trifunctional in nature. The presence of the benzotriazole moiety provides surface complexing properties, the azo linkage provides the colour and hence extra sensitivity and the nucleophilic amine group enables further functionalisation.