The effect of ginger (Zingiber officinale L.) liquid extract on growth, immune response, antioxidant defence mechanism, and general health of Holstein calves.

This study was carried out to examine the effects of ginger liquid extract (GLE) on the growth, immune response, antioxidative defence mechanism, and general health of Holstein calves. Sixteen calves (4-d old) were included in the experiment and randomly assigned to groups, and they were fed whole milk containing GLE at a rate of 0, 0.50, 0.72, and 1% of the milk amount consumed. Calves consuming 1% GLE were weaned at an earlier age and gained better body weight (BW) compared to the other groups. The group fed with 0.50% GLE consumed less daily starter than the other groups. The administration of GLE resulted in a non-significant decrease in fecal score (FS), the number of days with diarrhea (DDN), and illness (IDN) among the calves. Notably, the 1% GLE exhibited a significant inhibitory effect on the growth of E. coli, while its effect on the growth of other pathogenic bacteria was not statistically significant. Despite the non-significant reduction in malondialdehyde (MDA), total oxidative status (TOS), and oxidative stress index (OSI) values, the 1% GLE demonstrated support for antioxidative defence mechanism and immune response. The results indicated that 1% GLE can be effective in promoting the health of calves by enhancing their immune response and antioxidant capacity. This suggests that incorporating 1% GLE into their overall well-being, potentially leading to improved health outcomes and performance in calf rearing operations.

Effects of garlic powder-supplemented milk on growth and health indicators in Holstein calves.

This study aimed to investigate the effects of adding different doses of garlic powder (GP) into milk on the growth and health parameters of Holstein calves. Thirty Holstein calves were randomly divided into groups as follows: control (CON), T1 (10 mg GP/kg live weight (LW)), and T2 (30 mg GP/kg LW). Four days old calves were used as animal material. The calves were weaned when they consumed 800 g of starter for 3 consecutive days. The experiment was terminated when the calves reached 8-week age. Starter and water were available ad libitum. Both GP doses significantly decreased respiratory score, the number of illness days, and diarrheal days (p < 0.05). In addition, a significant improvement was observed in the general appearance of calves that received both GP doses (p < 0.05). Garlic powder created a significant decrease in oxidative stress index at 28 days old and total oxidative status at the end of the experiment (p < 0.05). Garlic powder was not significantly effective for the inhibition of pathogenic bacteria growth at the 28-day-old and the end of the experiment. The application of 30 mg/kg LW GP significantly reduced the incidence of cases such as diarrhea and respiratory diseases, which are common during the suckling period.

Chemical composition and biological activities of essential oils of seven Cultivated Apiaceae species.

The Apiaceae family contains many species used as food, spice and medicinal purposes. Different parts of plants including seeds could be used to obtain essential (EO) oils from members of the Apiaceae family. In the present study, EOs were components obtained through hydrodistillation from the seeds of anise (Pimpinella anisum), carrot (Daucus carota), celery (Apium graveolens), dill (Anethum graveolens), coriander (Coriandrum sativum), fennel (Foeniculum vulgare), and cumin (Cuminum cyminum). EO constituents were determined with Gas Chromatography/Mass Spectrometry (GC-MS) and Gas Chromatography/Flame Ionization Detector (GC-FID) and their antioxidant capacities were determined with the cupric reducing antioxidant capacity (CUPRAC) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) methods. The antimicrobial activity of EOs were tested against four pathogenic bacteria. Phenylpropanoids in anise (94.87%) and fennel (92.52%), oxygenated monoterpenes in dill (67.59%) and coriander (98.96%), monoterpene hydrocarbons in celery (75.42%), mono- (45.42%) and sesquiterpene- (43.25%) hydrocarbons in carrots, monoterpene hydrocarbon (34.30%) and aromatic hydrocarbons (32.92%) in cumin were the major compounds in the EOs. Anethole in anise and fennel, carotol in carrot, limonene in celery, carvone in dill, linalool in coriander, and cumin aldehyde in cumin were predominant compounds in these EOs. The high hydrocarbon content in cumin EO gave high CUPRAC activity (89.07 µmol Trolox g-1), and the moderate monoterpene hydrocarbon and oxygenated monoterpene content in dill EO resulted in higher DPPH activity (9.86 µmol Trolox g-1). The in vitro antibacterial activity of EOs against Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli was evaluated using the agar diffusion method and the minimum bactericidal concentration was determined. Coriander, cumin and dill EOs showed inhibitory effect against all tested strains except P. aeruginosa. While fennel and celery EOs were effective against E. coli and B. cereus strains, respectively, anise and carrot EOs did not show any antibacterial effect against the tested bacteria. Hierarchical Cluster Analysis (HCA) produced four groups based on EO constituents of seven species. The potential adoption of the cultivated Apiaceae species for EO extraction could be beneficial for the wild species that are endangered by over collection and consumption.

Dynamic changes occur in the cell wall composition and related enzyme activities during flower development in Rosa damascena.

The flowering period of oil-bearing rose is short and many physiological processes occur during flower development. Changes in the cell wall composition and associated enzyme activities are important as they allow cells to divide, differentiate and grow. In the present study, changes in seven cell wall components and six cell wall-related enzyme activities at five flower development stages were investigated and the relationships between these parameters and flowering were examined. Ash content did not change between stages I to II but decreased at later stages. Neutral detergent fiber (NDF), acid detergent fiber (ADF) and hemicellulose contents increased after stage I but did not change significantly at the other developmental periods. Total pectin content increased throughout flower development. An "increase-decrease" trend was observed in total cellulose content and a "decrease-increase" pattern in uronic acid content. The activities of both glycosidases (ß-galactosidase, ß-glucosidase and endoglucanase) and pectinases (pectin lyase, pectin methyl esterase and polygalacturonase) increased until stage IV and decreased significantly at stage V of flower development. Correlation analysis revealed 14 positive and one negative correlation with the studied parameters. Cell wall enzymes showed positive correlations with each other. Principal component analysis (PCA) showed that ADF, NDF and cellulose content were significantly altered at stage II of flower development, and significant changes occurred in all cell wall enzyme activities between stages III and V. Overall, blooming is correlated closely with increased pectin and decreased cellulose contents, and changes in cell wall glucosidase and pectin hydrolysis enzyme activities. These results show that cell wall modifying enzymes are part of the flower development process in oil-bearing rose. Therefore, remodeling of cell wall components in petals is a process of flower development.

Investigation of phenological, primary and secondary metabolites changes during flower developmental of Rosa damascena.

Oil-bearing rose is a very valuable member of the Rosa genus. Despite the importance of oil-bearing rose, metabolic changes during flower development are not well understood. Thus, the objective of this study was to investigate the changes in phenological, primary and secondary metabolites and their interactions at five developmental stages of oil-bearing rose. Flower width, flower and petal fresh weights, petal area and petal relative water content increased from bud stage to blooming stage, while flower length and sepal area increased only at early stages. Thirty-seven essential oil components were identified at different stages of petal development and nonadecane, ß-citronellol and n-heneicosane were the prevalent essential oil components regardless of stage. Sixteen fatty acids were identified and the amount of saturated fatty acids was higher than the mono and polyunsaturated fatty acids in all developmental stages. Eight organic acids were detected in petals and four of them (tartaric, malic, citric and succinic acids) showed significant changes, and total organic acids content decreased during flower development. Catechin and epicatechin were the most abundant phenolic compounds in petals. While total phenolic, flavonoid and free amino acids contents decreased during flower development, total free fatty acids content increased, but was not significant between the developmental stages. Correlation analysis between phenological traits and some metablolites revealed 20 significant correlations and 11 of which were positive. Results showed that flower development stages had significant effects on metabolite content and quality of products obtained, and significant shifts in metabolite type and content occurred at flower development stages III and IV.

Effect of harvesting hour on some physical and mechanical properties of Rosa damascena Mill.

BACKGROUND: Mechanization of Rosa damascena flower harvest is limited because of the asynchronous flowering period, the necessity for the harvest operation to take place in the early hour of the morning, and a lack of knowledge regarding the physical-mechanical properties of R. damascena. This study investigated the effect of harvesting hour on some physical-mechanical properties of R. damascena. RESULTS: The terminal velocity of the rose flower ranged from 4.00 to 2.38 m s(-1) . The highest picking force was found to be 6.29 N for the harvesting hour of 06:00 am. Tear forces of petal changed from 1.54 to 0.97 N for the harvesting hour of 06:00 am and 12:00 pm, respectively. The tension force of pedicel varied between 8.25 and 7.76 N. The weight of the rose flower was measured as an average of 1.61 and 1.50 g at 06:00 am and 12:00 pm, respectively. Horizontal and vertical projection areas of rose flowers were positively correlated with harvesting hour, with an R(2) of 0.97 and 0.75, respectively. Results showed that there was a minor loss in colour for both inner and outer surfaces of petals. CONCLUSION: The results of physical-mechanical properties of R. damascena obtained from this study should be considered for the operation of flower harvest mechanization.

The influence of harvest time on essential oil composition, phenolic constituents and antioxidant properties of Turkish oregano (Origanum onites L.).

BACKGROUND: The aim of this research was to determine essential oil composition, phenolic constituents and antioxidant properties of Turkish oregano (Origanum onites L.) leaves harvested during the months of June to September. RESULT: The maximum essential oil yield in the leaves appeared in the middle of July. The main components of oregano oil were carvacrol, thymol, gamma-terpinene, p-cymene, alpha-terpinene and alpha-pinene. Carvacrol was highest in the July harvest. The maximum extract yield was found in September. Oil distilled from early-season (June) harvested leaves had the highest antioxidant ability, expressed as low concentration providing 50% inhibition of free radical scavenging activity and high levels of reducing/antioxidant capacity. Twelve phenolic compounds of oregano extract were identified and the main components were found to be rosmarinic acid and acecetin. The maximum rosmarinic acid and acecetin were found in harvests of July and June, respectively. Total phenolic contents, free radical scavenging activities and reducing/antioxidant capacities were found to be highest in the July harvest. DISCUSSION: All yields, chemical compositions, free radical scavenging activities and reducing/antioxidant capacities of extracts and essential oils of Turkish oregano changed importantly depending on vegetative periods of growing season.