Direct Measurement of Hexacontatetrapole, E6 γ Decay from

The only proposed observation of a discrete, hexacontatetrapole (E6) transition in nature occurs from the T_{1/2}=2.54(2)-min decay of ^{53m}Fe. However, there are conflicting claims concerning its γ-decay branching ratio, and a rigorous interrogation of γ-ray sum contributions is lacking. Experiments performed at the Australian Heavy Ion Accelerator Facility were used to study the decay of ^{53m}Fe. For the first time, sum-coincidence contributions to the weak E6 and M5 decay branches have been firmly quantified using complementary experimental and computational methods. Agreement across the different approaches confirms the existence of the real E6 transition; the M5 branching ratio and transition rate have also been revised. Shell model calculations performed in the full fp model space suggest that the effective proton charge for high-multipole, E4 and E6, transitions is quenched to approximately two-thirds of the collective E2 value. Correlations between nucleons may offer an explanation of this unexpected phenomenon, which is in stark contrast to the collective nature of lower-multipole, electric transitions observed in atomic nuclei.

Electric Monopole Transition from the Superdeformed Band in

The electric monopole (E0) transition strength ρ^{2} for the transition connecting the third 0^{+} level, a "superdeformed" band head, to the "spherical" 0^{+} ground state in doubly magic ^{40}Ca is determined via e^{+}e^{-} pair-conversion spectroscopy. The measured value ρ^{2}(E0;0_{3}^{+}→0_{1}^{+})=2.3(5)×10^{-3} is the smallest ρ^{2}(E0;0^{+}→0^{+}) found in A<50 nuclei. In contrast, the E0 transition strength to the ground state observed from the second 0^{+} state, a band head of "normal" deformation, is an order of magnitude larger ρ^{2}(E0;0_{2}^{+}→0_{1}^{+})=25.9(16)×10^{-3}, which shows significant mixing between these two states. Large-scale shell-model (LSSM) calculations are performed to understand the microscopic structure of the excited states and the configuration mixing between them; experimental ρ^{2} values in ^{40}Ca and neighboring isotopes are well reproduced by the LSSM calculations. The unusually small ρ^{2}(E0;0_{3}^{+}→0_{1}^{+}) value is due to destructive interference in the mixing of shape-coexisting structures, which are based on several different multiparticle-multihole excitations. This observation goes beyond the usual treatment of E0 strengths, where two-state shape mixing cannot result in destructive interference.

Comprehensive Test of the Brink-Axel Hypothesis in the Energy Region of the Pygmy Dipole Resonance.

The validity of the Brink-Axel hypothesis, which is especially important for numerous astrophysical calculations, is addressed for ^{116,120,124}Sn below the neutron separation energy by means of three independent experimental methods. The γ-ray strength functions (GSFs) extracted from primary γ-decay spectra following charged-particle reactions with the Oslo method and with the shape method demonstrate excellent agreement with those deduced from forward-angle inelastic proton scattering at relativistic beam energies. In addition, the GSFs are shown to be independent of excitation energies and spins of the initial and final states. The results provide a critical test of the generalized Brink-Axel hypothesis in heavy nuclei, demonstrating its applicability in the energy region of the pygmy dipole resonance.

Radiative Width of the Hoyle State from γ-Ray Spectroscopy.

The cascading 3.21 and 4.44 MeV electric quadrupole transitions have been observed from the Hoyle state at 7.65 MeV excitation energy in ^{12}C, excited by the ^{12}C(p,p^{'}) reaction at 10.7 MeV proton energy. From the proton-γ-γ triple coincidence data, a value of Γ_{rad}/Γ=6.2(6)×10^{-4} was obtained for the radiative branching ratio. Using our results, together with Γ_{π}^{E0}/Γ from Eriksen et al. [Phys. Rev. C 102, 024320 (2020)PRVCAN2469-998510.1103/PhysRevC.102.024320] and the currently adopted Γ_{π}(E0) values, the radiative width of the Hoyle state is determined as Γ_{rad}=5.1(6)×10^{-3} eV. This value is about 34% higher than the currently adopted value and will impact models of stellar evolution and nucleosynthesis.

Effect of starvation and refeeding on the hepatopancreas of whiteleg shrimp Penaeus vannamei (Boone) using computer-assisted image analysis.

Under normal farming conditions, shrimp can experience starvation periods attributable to disease outbreaks or adverse environmental conditions. Starvation leads to significant morphological changes in the hepatopancreas (HP), being the main organ for absorption and storage of nutrients. In the literature, limited research has described the effect on the HP of periods of starvation followed by refeeding and none in whiteleg shrimp (Penaeus vannamei) using computer-assisted image analysis (CAIA). This study describes the effect of starvation and starvation followed by refeeding on the HP of whiteleg shrimp using CAIA. Visiopharm® software was used to quantify the following morphological parameters, measured as ratio to the total tissue area (TLA): total lumen area (TLA:TTA), haemocytic infiltration area in the intertubular spaces (HIA:TTA), B-cell vacuole area (VBA:TTA), lipid droplet area within R cells (LDA:TTA) and F-cell area (FCA:TTA). Significant changes were measured for HIA:TTA and LDA:TTA during starvation (increase in HIA:TTA associated with decrease in LDA:TTA) and starvation followed by refeeding (decrease in HIA:TTA associated with increase in LDA:TTA). In the future, HIA:TTA and LDA:TTA have the potential to be used in a pre-emptive manner to monitor the health of the HP, facilitate early diagnosis of diseases and study the pathophysiology of the organ.

Use of computer-assisted image analysis for semi-quantitative histology of the hepatopancreas in whiteleg shrimp Penaeus vannamei (Boone).

Despite the increasing use of novel molecular techniques in pathology, histology remains the standard method for monitoring tissue alterations and for assessing pathology. Histopathological evaluation is generally laborious and subjective with risk of discrepancies in semi-quantitative scoring between pathologists. In contrast, computer-assisted image analysis (CAIA) is potentially faster, more objective and thus suitable for routine screening. Limited research has been carried out on CAIA in crustacean histopathology, and the methods described were not fully automated. Therefore, the aim of this study was to develop CAIA in whiteleg shrimp (Penaeus vannamei) for the study of the hepatopancreas. Paraffin sections were immunohistochemically stained with monoclonal antibodies WSH8 against haemocytes and counterstained with Mayer's haematoxylin for detection of haemocytes and B-cell vacuoles, and modified toluidine blue protocol was used for detection of F-cells; frozen sections were stained with Oil Red O for detection of lipid droplets within R-cells. Visiopharm® software was used to develop and validated protocols for the quantification of morphological parameters (areas of haemocyte infiltration, F-cells, B-cell vacuoles, lipid droplets and their ratios to total tissue area and total lumen area). These protocols enable the future use of CAIA for determination of the nutritional and pathological condition of this organ.

Cranial morphology in the brachygnathic sheep.

BACKGROUND: Craniofacial morphology of sheep with phenotypically observed mandibular distocclusion was analysed using the multivariate techniques principle component analysis and cluster analysis in order to test whether different types of craniofacial malformations can be distinguished. RESULTS: The results showed 8 principal components with a variance of 82.72% in the database. The method creates new variables then used in the Cluster analysis indicating 7 clusters with 3 different facial types: Normal, prognathia inferior and brachygnathia inferior. CONCLUSION: The brachygnathic facial type was mainly characterised as a shortened mandible, the upper jaw is not significantly involved. The correlations to the temporomandibular joint were shown. Molar and premolar malocclusions were revealed in two of three Clusters. Phenotypical distocclusion was not a single criterion for the affected sheep.

Treatment with corticosteroids and the risk of anastomotic leakage following lower gastrointestinal surgery: a literature survey.

AIM: Background Anastomotic leakage is a serious complication in colorectal surgery. Treatment with corticosteroids is known to impair wound healing but their effect on the healing of a colorectal anastomosis remains unclear, and studies have reported conflicting results. Objective The aim of this study was to evaluate the current evidence regarding the effect of corticosteroids on the risk of anastomotic leakage following colorectal surgery. METHOD: Search strategy A systematic review was conducted following a search of PubMed and Embase. Selection criteria Inclusion criteria were studies published in English and involving humans. A minimum cohort of 50 patients was required and anastomoses involving the ileum, colon and rectum were included. Studies that investigated corticosteroids as a risk factor for anastomotic leakage were included regardless of the duration and the dose of corticosteroids. Data Collection and analysis A comparison was conducted between anastomotic leakage in noncorticosteroid- and corticosteroid-treated patients. The main outcome measure was the risk of anastomotic leakage. RESULTS: Twelve studies with a total of 9564 patients were included in the review. In total, 1034 patients received corticosteroids in the preoperative period, and 344 patients were diagnosed with anastomotic leakage, 70 of whom had received corticosteroids. Six of the 12 studies showed an increased risk for anastomotic leakage in the corticosteroid group. Overall, the anastomotic leakage rate was 6.77% (95% CI: 5.48-9.06) in the corticosteroid group and 3.26% (95% CI: 2.94-3.58) in the noncorticosteroid group. CONCLUSION: Caution should be shown in patients scheduled for lower gastrointestinal surgery with anastomosis.

Evidence for the dipole nature of the low-energy γ enhancement in 56Fe.

The γ-ray strength function of 56Fe has been measured from proton-γ coincidences for excitation energies up to ≈11 MeV. The low-energy enhancement in the γ-ray strength function, which was first discovered in the (3He,αγ)56Fe reaction, is confirmed with the (p,p'γ)56Fe experiment reported here. Angular distributions of the γ rays give for the first time evidence that the enhancement is dominated by dipole transitions.

A twelve-year longitudinal study of hearing thresholds among professional divers.

In this prospective study over twelve years, we have studied 30 young professional divers. The aim of the study was to see if changes in hearing thresholds were related to cumulative diving exposure. The study started at the beginning of the divers' education to become professional divers. Over the follow-up period the divers performed air-dives to shallow sea levels with a median number of 477 dives (range: 40-4458). The examination was performed by measuring air conduction thresholds in a sound treated booth. During follow-up, a significant reduction in auditory function was found at 0.25, 0.5, 2, 3 and 6 kHz for the right ear and 3, 4 and 6 kHz for the left ear. A reduction in hearing function associated with diving was found at 4 and 8 kHz (p < 0.01) both ears combined. Hearing impairment among this group of professional divers, with possible noise exposure, shows that hearing impairment is associated with their profession.

Periodontal disease in research beagle dogs--an epidemiological study.

OBJECTIVES: To estimate the prevalence and describe the extent and severity of periodontal disease and associated periodontal parameters in beagle dogs. METHODS: A full-mouth, site-specific examination was performed in 98 beagle dogs. Focus was placed on clinical attachment loss, pocket depth and bleeding on probing. RESULTS: The prevalence of clinical attachment loss greater than equal to 1 mm was 20 per cent in the one-year-old dogs, increasing to 84 per cent of the dogs aged more than three years. The number of sites affected with clinical attachment loss greater than equal to 1 mm showed a skewed distribution. The prevalence of clinical attachment loss greater than equal to 4 mm was only seven per cent. A probing pocket depth of 4+ mm was observed in 44 to 81 per cent of the dogs, depending on age. Also, the distribution of the number of deepened pockets/dog was skewed. The teeth most prone to clinical attachment loss greater than equal to 1 mm were the P2, the P3 and the P4 of the maxilla. The teeth most prone to pocket depth greater than equal to 4 mm were the maxillary canines. CLINICAL SIGNIFICANCE: Periodontal disease in terms of clinical attachment loss greater than equal to 1 mm and pocket depth greater than equal to 4 mm is common in beagle dogs, but the major disease burden is carried by only a few dogs. The prevalence increases with increased age but is high already at the age of two years.

Cloning and sequencing of cDNAs specifying a novel class of phosphoribosyl diphosphate synthase in Arabidopsis thaliana.

cDNAs specifying four active phosphoribosyl diphosphate synthase isozymes were isolated from an Arabidopsis thaliana cDNA library. In contrast to other phosphoribosyl diphosphate synthases the activity of two of the A. thaliana isozymes are independent of Pi. Amino acid sequence comparison and phylogenetic analysis indicate that these two isozymes belong to a novel class of phosphoribosyl diphosphate synthases.

Single port laparoscopic long-term tube gastrostomy in Göttingen minipigs.

Oral dosing by gavage is often used to test compounds in minipigs. This method is also used for certain nutritional studies that require exact dosing. This procedure may be stressful for the animal and requires the assistance of more than one technician. We investigated whether a gastrostomy tube could be placed and maintained in Göttingen minipigs using a single port laparoscopic technique. As part of another study, laparoscopic gastrostomy tube placement was performed in 12 Göttingen minipigs (32 ± 2 kg) under general anesthesia. The procedure involved single port laparoscopic visualization of the stomach and placement of a locking pigtail catheter into the fundus region of the stomach. The minipigs were followed for three weeks after surgery and macroscopic and microscopic tissue reactions were evaluated at necropsy. All catheters were successfully placed and were easy to use. At necropsy it was evident that the catheter had entered the stomach in the fundus region in 11/12 of the animals. In one animal the catheter had entered the antrum region. None of the animals developed leakage or clinically detectable reactions to the gastrostomy tube. Histopathologically, only discrete changes were observed. Single port laparoscopic tube gastrostomy with a locking pigtail catheter is safe, simple and reliable and is an appropriate alternative to, for example, percutaneous endoscopic gastrostomy, when long-term enteral delivery of pharmacological or nutritional compounds is needed. The use of the gastrostomy tube was easy and, based on subjective assessment, feeding was minimally stressful to the animals.

A comparison of anatomical lateral distal femoral angles obtained with four femoral axis methods in canine femora.

OBJECTIVES: To report the repeatability and reproducibility of four different anatomical proximal femoral axis (a-PFA) methods for measuring anatomical lateral distal femoral angle (a-LDFA), and to compare a-LDFA values produced by each method at three different femoral elevation angles. METHODS: Digital radiographs were obtained of seven dry canine femora at 0°, 12.5° and 25° elevations. Using image analysis software, landmarks defining four different a-PFA and the condylar axis were identified by two independent observers on two separate occasions. Corresponding a-LDFA were calculated for each femur, elevation and a-PFA. Repeatability and reproducibility parameters were calculated and compared statistically, along with the effect of technique and elevation on a-LDFA value. RESULTS: Interobserver repeatability coefficients were subjectively better for three of the a-PFA methods at 2° compared to the fourth at 3.1°. Median a-LDFA increased significantly (p ≤ 0.002) with increasing femoral elevation for all a-PFA methods, with a median increase of 3.3°. The median difference in a-LDFA between a-PFA methods yielding the highest and lowest measurements was 2.6° over all three elevations. CLINICAL SIGNIFICANCE: The combined effects of a-PFA choice, femoral elevation and measurement reproducibility may produce typical errors of ± 2.6°, which could have implications for the selection of candidates for corrective osteotomies. Clinicians need to be aware that values obtained with one method and femoral elevation may not be equivalent to values obtained with other methods or elevations.

Consequences of crown shortening canine teeth in Greenland sled dogs.

OBJECTIVES: To evaluate the consequences of crown shortening, focusing on the prevalence of pulp exposure and periapical pathology in Greenland sled dogs that had had their canine crowns shortened at an early age. METHODS: Five cadaver heads and 54 sled dogs underwent an oral examination for dental fractures and pulp exposure of canines. All canines were radiographed and evaluated for periapical pathology. RESULTS: The prevalence of canine pulp exposure in 12 (5 heads and 7 dogs) crown shortened dogs was 91 · 7%, and 21 · 3% in 47 not-crown shortened dogs. A significant (P < 0 · 001) risk of pulp exposure of the canines in the crown shortened group compared to the not-crown shortened group was seen with a relative risk of 4 · 3 on a dog basis and a relative risk of 12 · 2 on a tooth basis. In dogs with pulp exposure of canines (n = 51) the prevalence of periapical pathology was 82 · 4%, but only 0 · 8% in dogs without pulp exposure (n = 133) resulting in a significant (relative risk, 109 · 5; P < 0 · 001) risk of periapical pathology in teeth with pulp exposure compared to teeth without pulp exposure. CLINICAL SIGNIFICANCE: The high risk of periapical pathology observed in teeth with pulp exposure confirms that these teeth should not be neglected in affected dogs.

Ultrastructural features of secretory cells in the bovine oviduct epithelium.

The non-ciliated (NC) cells of the bovine oviduct epithelium, have been shown to release embryotrophic substances to the oviduct lumen. The aim of the present study was to investigate the ultrastructure, focusing on aspects of the secretory machinery, of NC cells in different segments of the oviduct during and after transoviduct migration of zygotes and embryos. Dairy heifers (n = 8) were superovulated with an ECG/cloprostenol regimen, and the time of ovulation was estimated by ultrasound scanning. Samples from the infundibulum, ampulla, isthmus and uterotubal-junction of the oviduct were surgically collected from animals at 19-96 h and 7 1/2-8 1/2 days after ovulation and processed for transmission electron microscopy, following standard procedures. The NC cells contained characteristic membrane-bound secretory granules composed of a lamellar cortex encaging an amorphous medulla. The two components could still be recognized during extrusion of the granule content into the oviduct lumen by exocytosis. During granulogenesis, small maturing granules without the lamellar structure were observed, but distinct condensing vacuoles were absent. An abundance of granules was found in the early versus the late group. In both groups the uterotubual junction was almost free of granules. This segment, on the contrary, was characterized by the presence of primary and secondary lysosome-like bodies. In the early group the intracellular location of the granules varied between oviduct segments.(ABSTRACT TRUNCATED AT 250 WORDS)

Frequency of dental visits in Sweden during 1974-77.

A longitudinal study of the frequency of dental visits has been conducted on a sample of 1302 individuals aged 20-60 years. These individuals had previously been interviewed regarding their dental care habits. They had also (subsequent to the interview) been clinically examined by a dentist. The results presented are based on the official dental care consumption statistics in Sweden, and thus differ from the information obtained from the interviews. In all age groups, women had a higher frequency of dental visits than men. The average number of courses of treatment carried out over a 4-year period was 2.0. Only 23% of the individuals received treatment once a year or more often, whereas 79% received treatment at least once during a period of 4 years. These figures were lower than those reported in the interviews. Individuals with a higher number of courses of treatment had also had a higher frequency of dental visits before, had been called in by their dentist for check-ups, had a higher income and knew more about teeth and their care, used oral hygiene aids daily and were living in metropolitan areas. Younger people with many remaining teeth and those with many decayed teeth had had a higher frequency of courses of treatment.

Treatment of caries and periodontitis in Sweden in 1974-77.

During 1974 and 1975 a sample of individuals in Sweden between 20 and 60 years were interviewed about dental care habits. 938 of them were then clinically examined. An estimation of the time needed for treatment of dental caries and periodontitis was based upon the clinical examination. Dental status and the estimated treatment time were then related to the dental care carried out during one period of 18 months and one of 48 months after the clinical examination. For these calculations, information on dental care was acquired from the patient register of the National Social Insurance Board. The results derived from the analyses showed that the requisite time for treating caries related well to the treatment executed. On the other hand the time for treating periodontal disease showed a poorer agreement with what could have been expected from the clinical periodontal status. The treatment time devoted to periodontal disease was considerably higher in the metropolitan areas, with their higher density of dentists, than in other parts of Sweden. Thus, dental care in Sweden between 1974 and 1978 concentrated primarily on restorative procedures while a progression of periodontal disease was largely ignored by dental practitioners.