RESUMO
In this study, a virtual screening procedure was applied to identify new potential nt-MGAM inhibitors as a possible medication for type 2 diabetes. To this aim, a series of salacinol analogues were first investigated by docking analysis for their binding to the X-ray structure of the biological target nt-MGAM. Key interactions for ligand binding into the receptor active site were identified which shared common features to those found for other known inhibitors, which strengthen the results of this study. 3D QSAR model was then built and showed to be statistically significant and with a good predictive power for the training (R2â¯=â¯0.99, SDâ¯=â¯0.17, Fâ¯=â¯555.3 and Nâ¯=â¯27) and test set (Q2â¯=â¯0.81, Pearson(r)â¯=â¯0.92, RMSEâ¯=â¯0.52, Nâ¯=â¯08). The model was then used to virtually screen the ZINC database with the aim of identifying novel chemical scaffolds as potential nt-MGAM inhibitors. Further, in silico predicted ADME properties were investigated for the most promising molecules. The outcome of this investigation sheds light on the molecular characteristics of the binding of salacinol analogues to nt-MGAM enzyme and identifies new possible inhibitors which have the potential to be developed into drugs, thus significantly contributing to the design and optimization of therapeutic strategies against type 2 diabetes.
Assuntos
Inibidores de Glicosídeo Hidrolases/química , Hipoglicemiantes/química , Álcoois Açúcares/química , Ésteres do Ácido Sulfúrico/química , alfa-Glucosidases/química , Diabetes Mellitus Tipo 2/tratamento farmacológico , Avaliação Pré-Clínica de Medicamentos , Ligação de Hidrogênio , Ligantes , Simulação de Acoplamento Molecular , Relação Quantitativa Estrutura-AtividadeRESUMO
In this communication we report on the self-organising properties of a novel asymmetric bolaphile composed of sugar and steroidal units. The material was found to exhibit unusual phase changes in that it formed a cubic phase upon heating, whereas upon cooling it formed a lamellar phase. This change in property was ascribed to kinetic versus thermodynamic behaviour at the phase transition to the liquid.