Motor resonance in monkey parietal and premotor cortex during action observation: Influence of viewing perspective and effector identity.

Observing others performing motor acts like grasping has been shown to elicit neural responses in the observer`s parieto-frontal motor network, which typically becomes active when the observer would perform these actions him/herself. While some human studies suggested strongest motor resonance during observation of first person or egocentric perspectives compared to third person or allocentric perspectives, other research either report the opposite or did not find any viewpoint-related preferences in parieto-premotor cortices. Furthermore, it has been suggested that these motor resonance effects are lateralized in the parietal cortex depending on the viewpoint and identity of the observed effector (left vs right hand). Other studies, however, do not find such straightforward hand identity dependent motor resonance effects. In addition to these conflicting findings in human studies, to date, little is known about the modulatory role of viewing perspective and effector identity (left or right hand) on motor resonance effects in monkey parieto-premotor cortices. Here, we investigated the extent to which different viewpoints of observed conspecific hand actions yield motor resonance in rhesus monkeys using fMRI. Observing first person, lateral and third person viewpoints of conspecific hand actions yielded significant activations throughout the so-called action observation network, including STS, parietal and frontal cortices. Although region-of-interest analysis of parietal and premotor motor/mirror neuron regions AIP, PFG and F5, showed robust responses in these regions during action observation in general, a clear preference for egocentric or allocentric perspectives was not evident. Moreover, except for lateralized effects due to visual field biases, motor resonance in the monkey brain during grasping observation did not reflect hand identity dependent coding.

Functional MRI Responses to Passive, Active, and Observed Touch in Somatosensory and Insular Cortices of the Macaque Monkey.

Neurophysiological data obtained in primates suggests that merely observing others' actions can modulate activity in the observer's motor cortices. In humans, it has been suggested that these multimodal vicarious responses extend well beyond the motor cortices, including somatosensory and insular brain regions, which seem to yield vicarious responses when witnessing others' actions, sensations, or emotions (Gazzola and Keysers, 2009). Despite the wealth of data with respect to shared action responses in the monkey motor system, whether the somatosensory and insular cortices also yield vicarious responses during observation of touch remains largely unknown. Using independent tactile and motor fMRI localizers, we first mapped the hand representations of two male monkeys' primary (SI) and secondary (SII) somatosensory cortices. In two subsequent visual experiments, we examined fMRI brain responses to (1) observing a conspecific's hand being touched or (2) observing a human hand grasping or mere touching an object or another human hand. Whereas functionally defined "tactile SI" and "tactile SII" showed little involvement in representing observed touch, vicarious responses for touch were found in parietal area PFG, consistent with recent observations in humans (Chan and Baker, 2015). Interestingly, a more anterior portion of SII, and posterior insular cortex, both of which responded when monkeys performed active grasping movements, also yielded visual responses during different instances of touch observation.SIGNIFICANCE STATEMENT Common coding of one's own and others' actions, sensations, and emotions seems to be widespread in the brain. Although it is currently unclear to what extent human somatosensory cortices yield vicarious responses when observing touch, even less is known about the presence of similar vicarious responses in monkey somatosensory cortex. We therefore localized monkey somatosensory hand representations using fMRI and investigated whether these regions yield vicarious responses while observing various instances of touch. Whereas "tactile SI and SII" did not elicit responses during touch observation, a more anterior portion of SII, in addition to area PFG and posterior insular cortex, all of which responded during monkeys' own grasping movements, yielded vicarious responses during observed touch.

Decoding the content of working memory in school-aged children.

Developmental improvements in working memory (WM) maintenance predict many real-world outcomes, including educational attainment. It is thus critical to understand which WM mechanisms support these behavioral improvements, and how WM maintenance strategies might change through development. One challenge is that specific WM neural mechanisms cannot easily be measured behaviorally, especially in a child population. However, new multivariate decoding techniques have been designed, primarily in adult populations, that can sensitively decode the contents of WM. The goal of this study was to deploy multivariate decoding techniques known to decode memory representations in adults to decode the contents of WM in children. We created a simple computerized WM game for children, in which children maintained different categories of information (visual, spatial or verbal). We collected electroencephalography (EEG) data from 20 children (7-12-year-olds) while they played the game. Using Multivariate Pattern Analysis (MVPA) on children's EEG signals, we reliably decoded the category of the maintained information during the sensory and maintenance period. Across exploratory reliability and validity analyses, we examined the robustness of these results when trained on less data, and how these patterns generalized within individuals throughout the testing session. Furthermore, these results matched theory-based predictions of WM across individuals and across ages. Our proof-of-concept study proposes a direct and age-appropriate potential alternative to exclusively behavioral WM maintenance measures in children. Our study demonstrates the utility of MVPA to measure and track the uninstructed representational content of children's WM. Future research could use our technique to investigate children's WM maintenance and strategies.