Analytical evaluation of Sysmex UF-1000i for flow cytometric analysis of peritoneal fluid.

OBJECTIVE: To evaluate analytical performance of Sysmex UF-1000i for peritoneal fluid analysis. METHODS: Functional sensitivity, imprecision, linearity and comparison studies were performed on peritoneal fluids. RESULTS: Total imprecision was 1.6-4.7%, functional sensitivity 27/µL for white blood cell (WBC) and 32/µL for total nucleated cell (TNC) count. Linearity was excellent up to 983 cell/µL, carry-over <0.2%, correlation with manual microscopy always greater than 0.992. CONCLUSION: The instrument exhibited optimal performance at the conventional WBC diagnostic thresholds.