RESUMO
Autoantibodies raised against tumor-associated antigens have shown high promise as clinical biomarkers for reliable diagnosis, prognosis, and therapy monitoring of cancer. An electrochemical disposable biosensor for the specific and sensitive determination of p53-specific autoantibodies has been developed for the first time in this work. This biosensor involves the use of magnetic microcarriers (MBs) modified with covalently immobilized HaloTag fusion p53 protein as solid supports for the selective capture of specific autoantibodies. After magnetic capture of the modified MBs onto screen-printed carbon working electrodes, the amperometric signal using the system hydroquinone/H2O2 was related to the levels of p53-autoantibodies in the sample. The biosensor was applied for the analysis of sera from 24 patients with high-risk of developing colorectal cancer and 6 from patients already diagnosed with colorectal (4) and ovarian (2) cancer. The developed biosensor was able to determine p53 autoantibodies with a sensitivity higher than that of a commercial standard ELISA using a just-in-time produced protein in a simpler protocol with less sample volume and easily miniaturized and cost-effective instrumentation.
Assuntos
Autoanticorpos/sangue , Autoanticorpos/imunologia , Técnicas Biossensoriais/métodos , Neoplasias/sangue , Neoplasias/imunologia , Proteína Supressora de Tumor p53/imunologia , Técnicas Biossensoriais/instrumentação , Neoplasias Colorretais/sangue , Neoplasias Colorretais/imunologia , Técnicas Eletroquímicas/instrumentação , Técnicas Eletroquímicas/métodos , Desenho de Equipamento , Feminino , Humanos , Proteínas Imobilizadas/química , Proteínas Imobilizadas/imunologia , Imunidade Humoral , Limite de Detecção , Biópsia Líquida/instrumentação , Biópsia Líquida/métodos , Modelos Moleculares , Neoplasias Ovarianas/sangue , Neoplasias Ovarianas/imunologia , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/imunologia , Proteína Supressora de Tumor p53/químicaRESUMO
STUDY OBJECTIVE: To analyze factors that contribute to long-term success of loop endometrial resection (ER). DESIGN: Observational cohort study (Canadian Task Force classification II-2). SETTING: Tertiary care university hospital. PATIENTS: Two hundred eighty-six premenopausal women with menometrorrhagia resistant to medical treatment. INTERVENTION: Loop endometrial resection. MEASUREMENTS AND MAIN RESULTS: Mean follow-up was 47 months. Life table analysis with log rank test was determined to calculate the risk of hysterectomy after ER. Patient age, length of follow-up, adenomyosis, and uterine size were analyzed independently. More than 75% of patients benefited from the procedure, avoiding hysterectomy after 5 years. CONCLUSION: Length of follow-up, patient age, and the presence of adenomyosis strongly influence the long-term success of ER. The procedure should be considered an intermediate step between medical treatment and hysterectomy.