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1.
J Asian Nat Prod Res ; 25(1): 53-60, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-35446749

RESUMO

Two new sesquiterpenoids (1 and 3), one new natural product (2), and two known compounds (4 and 5) were isolated from the leaves of Chimonanthus nitens. Their structures were elucidated by spectroscopic analysis, and the absolute configuration of compound 3 was determined by the X-ray single-crystal diffraction analysis. The cytotoxicity of compounds 1-5 was evaluated at three concentrations on two human breast cancer cell lines (MDA-MB-468 and MDA-MB-231) by MTT assay. As a result, we found that the cytotoxicity was weak even with a concentration of these compounds up to 100 µM.


Assuntos
Calycanthaceae , Medicamentos de Ervas Chinesas , Sesquiterpenos , Humanos , Folhas de Planta/química , Medicamentos de Ervas Chinesas/química , Calycanthaceae/química , Sesquiterpenos/farmacologia , Estrutura Molecular
2.
Zhongguo Zhong Yao Za Zhi ; 42(20): 3938-3944, 2017 Oct.
Artigo em Zh | MEDLINE | ID: mdl-29243431

RESUMO

To study sesquiterpenes with anti-metastasis breast cancer activity from Chloranthus henryi, ten sesquiterpenes ,zedoarofuran (1), chlorajapolide D (2), 4ß, 8ß-dihydroxy-5α(H)-eudesm-7(11)-en-8, 12-olide (3), curcolonol (4), lasianthuslactone A (5), chlomultin C (6), (1E,4Z)-8-hydroxy-6-oxogermacra-1(10), 4, 7(11) -trieno-12, 8-lactone (7), shizukanolide E (8) , shizukanolide F (9) , 9α-hydroxycurcolonol (10), and five bis-sesquiterpenes, shizukaol B (11), shizukaol C (12) , cycloshizukaol A (13) , sarcandrolide B (14) , henriol A(15), were isolated by using different kinds of column chromatography methods from the ethyl acetate part of Ch.henryi and their structures were identified based on spectroscopic methods. Compounds 2, 8, 9, and 10 were obtained from the genus Chloranthus for the first time. Compounds 2, 5, 8-10, 12,and 14 were obtained from this plant for the first time. Some isolated compounds were subjected to evaluate the anti-metastasis breast cancer activity by using pharmacological methods, and only compounds 4, 11, and 12 were potent active.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Neoplasias da Mama/patologia , Sesquiterpenos/farmacologia , Traqueófitas/química , Antineoplásicos Fitogênicos/isolamento & purificação , Neoplasias da Mama/tratamento farmacológico , Linhagem Celular Tumoral , Humanos , Estrutura Molecular , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Sesquiterpenos/isolamento & purificação
3.
Zhongguo Zhong Yao Za Zhi ; 39(1): 10-3, 2014 Jan.
Artigo em Zh | MEDLINE | ID: mdl-24754160

RESUMO

Projects which supported by National Natural Science Foundation of China (NSFC) in discipline of pharmacology of Chinese medicine between 2010 to 2013 financial years were reviewed. Based on these research items, new features and problems were summarized in this field.


Assuntos
Fundações/economia , Medicina Tradicional Chinesa/economia , Disciplinas das Ciências Naturais/economia , Pesquisa/economia , China , Humanos
4.
Fitoterapia ; 176: 106000, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38729248

RESUMO

Five new characteristic cembrane-type diterpenoids (olibacartiols A-E, 1-5) were acquired from the gum resin of Boswellia carterii. The structures of these diterpenoids were characterized by detailed spectroscopic analysis, and compounds 1-3 were unambiguously confirmed by single-crystal X-ray diffraction experiments. The anti-inflammatory activities of the isolated compounds were evaluated using LPS-induced BV2 cell model and compounds 2-5 showed moderate NO inhibitory effects with IC50 values of 8.84 ± 1.02, 9.82 ± 1.95, 9.75 ± 2.24, and 7.39 ± 1.24 µM, respectively.


Assuntos
Anti-Inflamatórios , Boswellia , Diterpenos , Óxido Nítrico , Compostos Fitoquímicos , Resinas Vegetais , Diterpenos/farmacologia , Diterpenos/isolamento & purificação , Diterpenos/química , Boswellia/química , Óxido Nítrico/metabolismo , Estrutura Molecular , Animais , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/isolamento & purificação , Anti-Inflamatórios/química , Resinas Vegetais/química , Camundongos , Compostos Fitoquímicos/farmacologia , Compostos Fitoquímicos/isolamento & purificação , Linhagem Celular , China , Gomas Vegetais/química , Gomas Vegetais/farmacologia
5.
J Sep Sci ; 35(3): 384-91, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22258808

RESUMO

A novel method, micro-solid-phase extraction based on membrane-protected molecularly imprinted polymer, was developed to extract hyperoside and isoquercitrin in rat plasma. Synthesized hyperoside MIPs were packed in a porous polyether sulfone membrane envelope to perform extraction. The parameters sorbent materials, membrane types, extraction time and desorption conditions were optimized for micro-solid-phase extraction. Under the optimal conditions, correlation coefficients, 0.998 and 0.999, were obtained for hyperoside and isoquercitrin, respectively, with the linear range between 1 and 120 µg/mL. The absolute extraction recoveries from 84.5 to 89.3% were found. The method detection limits of hyperoside and isoquercitrin were 0.24 and 0.22 µg/mL, respectively. Compared with traditional methods, solid-phase extraction, liquid-liquid extraction and protein precipitation, the developed method was simple, highly efficient for extraction, environmentally friendly, and particularly suitable for complex biological samples.


Assuntos
Quercetina/análogos & derivados , Extração em Fase Sólida , Animais , Cromatografia Líquida de Alta Pressão , Quercetina/sangue , Quercetina/química , Ratos
6.
Fitoterapia ; 154: 105019, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34403777

RESUMO

Six new cadinane-type sesquiterpenoids, named Chimnitensin A-F (1-6) were isolated from the leaves of Chimonanthus nitens Oliv. Their structures were elucidated by comprehensive spectroscopic analyses and comparison with structurally related known analogues. In vitro MTT assay showed that all six compounds had cytotoxicity against two selected human breast cancer cell lines (MDA-MB-468 and MDA-MB-231), which indicate their potential of developing into anticancer drugs.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Calycanthaceae/química , Sesquiterpenos Policíclicos/farmacologia , Antineoplásicos Fitogênicos/isolamento & purificação , Linhagem Celular Tumoral , China , Humanos , Estrutura Molecular , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Folhas de Planta/química , Sesquiterpenos Policíclicos/isolamento & purificação
7.
Dig Dis Sci ; 54(8): 1643-50, 2009 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-19034653

RESUMO

Reactive oxygen species (ROS) are increased in inflammatory bowel disease (IBD) and have been implicated as mediators of intestinal inflammation. We investigated the hypothesis that N-acetylcysteine (NAC) as a glutathione (GSH) precursor attenuates disease progression in a murine dextran sodium sulfate (DSS)-induced colitis model. A colitis model was induced by adding 5% DSS into the drinking water for 7 days. BALB/c mice were injiciatur enema with saline, 5-ASA, N-acetylcysteine, respectively, and free drinking water as control group. DSS-treated mice developed severe colitis as shown by bloody diarrhea, weight loss, and pathologic involvement. Colon lengths were significantly decreased in DSS-treated mice with decreased GSH activity too (P < 0.01). ROS in the colon, the level of interleukin 1 beta (IL-1 beta) in colonic mucosa, serum tumor necrosis factor a (TNF-alpha), MPO, and MDA were significantly increased in DSS-treated animals (P < 0.01), with decreased PON1 activity (P < 0.01). However, NAC significantly decreased colonic MPO activity, ROS, TNF-alpha and IL-1 beta levels and increased PON1 activity and GSH concentration. Moreover, NAC attenuated the macroscopic colonic damage and the histopathologic changes-induced by DSS while similar to 5-ASA group. These results suggest that NAC may be effective in the treatment of colitis through its up-regulating PON1 and scavenging oxygen-derived free radicals.


Assuntos
Acetilcisteína/farmacologia , Antioxidantes/farmacologia , Arildialquilfosfatase/metabolismo , Colite/induzido quimicamente , Colite/metabolismo , Sulfato de Dextrana/efeitos adversos , Regulação para Cima/efeitos dos fármacos , Acetilcisteína/uso terapêutico , Animais , Antioxidantes/uso terapêutico , Colite/prevenção & controle , Colo/efeitos dos fármacos , Colo/metabolismo , Colo/patologia , Modelos Animais de Doenças , Regulação para Baixo/efeitos dos fármacos , Glutationa/metabolismo , Interleucina-1beta/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Estresse Oxidativo/efeitos dos fármacos , Peroxidase/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Índice de Gravidade de Doença , Fator de Necrose Tumoral alfa/metabolismo
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