Transcriptome analysis reveals critical factors for survival after adenovirus serotype 4 infection.

Fowl adenovirus serotype-4 (FAdV-4) is highly lethal to poultry, making it one of the leading causes of economic losses in the poultry industry. However, a small proportion of poultry can survive after FAdV-4 infection. It is unclear whether there are genetic factors that protect chickens from FAdV-4 infection. Therefore, the livers from chickens uninfected with FAdV-4 (Normal), dead after FAdV-4 infection (Dead) or surviving after FAdV-4 infection (Survivor) were collected for RNA-seq, and 2,649 differentially expressed genes (DEGs) were identified. Among these, many immune-related cytokines and chemokines were significantly upregulated in the Dead group compared with the Survivor group, which might indicate that death is related to an excessive inflammatory immune response (cytokine storm). Subsequently, the KEGG results for DEGs specifically expressed in each comparison group indicated that cell cycle and apoptosis-related DEGs were upregulated and metabolism-related DEGs were downregulated in the Dead group, which also validated the reliability of the samples. Furthermore, GO and KEGG results showed DEGs expressed in all three groups were mainly associated with cell cycle. Among them, BRCA1, CDK1, ODC1, and MCM3 were screened as factors that might influence FAdV-4 infection. The qPCR results demonstrated that these 4 factors were not only upregulated in the Dead group but also significantly upregulated in the LMH cells after 24 h infection by FAdV-4. Moreover, interfering with BRCA1, CDK1, ODC1, and MCM3 significantly attenuated viral replication of FAdV-4. And interfering of BRCA1, CDK1, and MCM3 had more substantial hindering effects. These results provided novel insights into the molecular changes following FAdV-4 infection but also shed light on potential factors driving the survival of FAdV-4 infection in chickens.

Recognition of pornographic web pages by classifying texts and images.

With the rapid development of the World Wide Web, people benefit more and more from the sharing of information. However, Web pages with obscene, harmful, or illegal content can be easily accessed. It is important to recognize such unsuitable, offensive, or pornographic Web pages. In this paper, a novel framework for recognizing pornographic Web pages is described. A C4.5 decision tree is used to divide Web pages, according to content representations, into continuous text pages, discrete text pages, and image pages. These three categories of Web pages are handled, respectively, by a continuous text classifier, a discrete text classifier, and an algorithm that fuses the results from the image classifier and the discrete text classifier. In the continuous text classifier, statistical and semantic features are used to recognize pornographic texts. In the discrete text classifier, the naive Bayes rule is used to calculate the probability that a discrete text is pornographic. In the image classifier, the object's contour-based features are extracted to recognize pornographic images. In the text and image fusion algorithm, the Bayes theory is used to combine the recognition results from images and texts. Experimental results demonstrate that the continuous text classifier outperforms the traditional keyword-statistics-based classifier, the contour-based image classifier outperforms the traditional skin-region-based image classifier, the results obtained by our fusion algorithm outperform those by either of the individual classifiers, and our framework can be adapted to different categories of Web pages.

Semantic-based surveillance video retrieval.

Visual surveillance produces large amounts of video data. Effective indexing and retrieval from surveillance video databases are very important. Although there are many ways to represent the content of video clips in current video retrieval algorithms, there still exists a semantic gap between users and retrieval systems. Visual surveillance systems supply a platform for investigating semantic-based video retrieval. In this paper, a semantic-based video retrieval framework for visual surveillance is proposed. A cluster-based tracking algorithm is developed to acquire motion trajectories. The trajectories are then clustered hierarchically using the spatial and temporal information, to learn activity models. A hierarchical structure of semantic indexing and retrieval of object activities, where each individual activity automatically inherits all the semantic descriptions of the activity model to which it belongs, is proposed for accessing video clips and individual objects at the semantic level. The proposed retrieval framework supports various queries including queries by keywords, multiple object queries, and queries by sketch. For multiple object queries, succession and simultaneity restrictions, together with depth and breadth first orders, are considered. For sketch-based queries, a method for matching trajectories drawn by users to spatial trajectories is proposed. The effectiveness and efficiency of our framework are tested in a crowded traffic scene.

A system for learning statistical motion patterns.

Analysis of motion patterns is an effective approach for anomaly detection and behavior prediction. Current approaches for the analysis of motion patterns depend on known scenes, where objects move in predefined ways. It is highly desirable to automatically construct object motion patterns which reflect the knowledge of the scene. In this paper, we present a system for automatically learning motion patterns for anomaly detection and behavior prediction based on a proposed algorithm for robustly tracking multiple objects. In the tracking algorithm, foreground pixels are clustered using a fast accurate fuzzy K-means algorithm. Growing and prediction of the cluster centroids of foreground pixels ensure that each cluster centroid is associated with a moving object in the scene. In the algorithm for learning motion patterns, trajectories are clustered hierarchically using spatial and temporal information and then each motion pattern is represented with a chain of Gaussian distributions. Based on the learned statistical motion patterns, statistical methods are used to detect anomalies and predict behaviors. Our system is tested using image sequences acquired, respectively, from a crowded real traffic scene and a model traffic scene. Experimental results show the robustness of the tracking algorithm, the efficiency of the algorithm for learning motion patterns, and the encouraging performance of algorithms for anomaly detection and behavior prediction.

Learning sparse kernel classifiers for multi-instance classification.

We propose a direct approach to learning sparse kernel classifiers for multi-instance (MI) classification to improve efficiency while maintaining predictive accuracy. The proposed method builds on a convex formulation for MI classification by considering the average score of individual instances for bag-level prediction. In contrast, existing formulations used the maximum score of individual instances in each bag, which leads to nonconvex optimization problems. Based on the convex MI framework, we formulate a sparse kernel learning algorithm by imposing additional constraints on the objective function to enforce the maximum number of expansions allowed in the prediction function. The formulated sparse learning problem for the MI classification is convex with respect to the classifier weights. Therefore, we can employ an effective optimization strategy to solve the optimization problem that involves the joint learning of both the classifier and the expansion vectors. In addition, the proposed formulation can explicitly control the complexity of the prediction model while still maintaining competitive predictive performance. Experimental results on benchmark data sets demonstrate that our proposed approach is effective in building very sparse kernel classifiers while achieving comparable performance to the state-of-the-art MI classifiers.

MILIS: multiple instance learning with instance selection.

Multiple instance learning (MIL) is a paradigm in supervised learning that deals with the classification of collections of instances called bags. Each bag contains a number of instances from which features are extracted. The complexity of MIL is largely dependent on the number of instances in the training data set. Since we are usually confronted with a large instance space even for moderately sized real-world data sets applications, it is important to design efficient instance selection techniques to speed up the training process without compromising the performance. In this paper, we address the issue of instance selection in MIL. We propose MILIS, a novel MIL algorithm based on adaptive instance selection. We do this in an alternating optimization framework by intertwining the steps of instance selection and classifier learning in an iterative manner which is guaranteed to converge. Initial instance selection is achieved by a simple yet effective kernel density estimator on the negative instances. Experimental results demonstrate the utility and efficiency of the proposed approach as compared to the state of the art.

Mixing linear SVMs for nonlinear classification.

In this paper, we address the problem of combining linear support vector machines (SVMs) for classification of large-scale nonlinear datasets. The motivation is to exploit both the efficiency of linear SVMs (LSVMs) in learning and prediction and the power of nonlinear SVMs in classification. To this end, we develop a LSVM mixture model that exploits a divide-and-conquer strategy by partitioning the feature space into subregions of linearly separable datapoints and learning a LSVM for each of these regions. We do this implicitly by deriving a generative model over the joint data and label distributions. Consequently, we can impose priors on the mixing coefficients and do implicit model selection in a top-down manner during the parameter estimation process. This guarantees the sparsity of the learned model. Experimental results show that the proposed method can achieve the efficiency of LSVMs in the prediction phase while still providing a classification performance comparable to nonlinear SVMs.