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1.
Ann Bot ; 132(5): 1033-1050, 2023 11 30.
Artigo em Inglês | MEDLINE | ID: mdl-37850481

RESUMO

Anthocyanin composition is responsible for the red colour of grape berries and wines, and contributes to their organoleptic quality. However, anthocyanin biosynthesis is under genetic, developmental and environmental regulation, making its targeted fine-tuning challenging. We constructed a mechanistic model to simulate the dynamics of anthocyanin composition throughout grape ripening in Vitis vinifera, employing a consensus anthocyanin biosynthesis pathway. The model was calibrated and validated using six datasets from eight cultivars and 37 growth conditions. Tuning the transformation and degradation parameters allowed us to accurately simulate the accumulation process of each individual anthocyanin under different environmental conditions. The model parameters were robust across environments for each genotype. The coefficients of determination (R2) for the simulated versus observed values for the six datasets ranged from 0.92 to 0.99, while the relative root mean square errors (RRMSEs) were between 16.8 and 42.1 %. The leave-one-out cross-validation for three datasets showed R2 values of 0.99, 0.96 and 0.91, and RRMSE values of 28.8, 32.9 and 26.4 %, respectively, suggesting a high prediction quality of the model. Model analysis showed that the anthocyanin profiles of diverse genotypes are relatively stable in response to parameter perturbations. Virtual experiments further suggested that targeted anthocyanin profiles may be reached by manipulating a minimum of three parameters, in a genotype-dependent manner. This model presents a promising methodology for characterizing the temporal progression of anthocyanin composition, while also offering a logical foundation for bioengineering endeavours focused on precisely adjusting the anthocyanin composition of grapes.


Assuntos
Vitis , Vinho , Vitis/genética , Antocianinas/análise , Antocianinas/metabolismo , Frutas/genética , Frutas/metabolismo , Vinho/análise
2.
Phytopathology ; 112(7): 1575-1583, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35113670

RESUMO

Brown rot in stored stone fruits, caused by Monilinia spp., may be due to preharvest and storage factors, but the combined effect of these factors has yet to be investigated. We set up two experiments to monitor the progression of brown rot during the storage of nectarines subjected to various preharvest and storage conditions. We assessed the effects of different agricultural practices (irrigation regimen × fruit load) and harvest dates on brown rot progress during storage in 2018 and the effect of different storage temperatures in 2019. We found that the cumulative incidence of brown rot during storage increased with individual fruit mass, which was influenced by agricultural practices, and for later harvest dates. It also increased with storage temperature. We observed that during storage no secondary infections developed in nectarines not in direct contact with fruits infected with Monilinia laxa. These findings led to the identification of candidate variables describing the brown rot risk on nectarines during storage, such as individual fruit mass, meteorological conditions before fruit harvest, prevalence of brown rot at harvest, and storage temperature. We used these variables to build a mathematical model for estimating the time-to-appearance of brown rot symptoms in stored nectarines. This model fitted the experimental data well, highlighting the need to pay greater attention to the interaction between preharvest and storage conditions. This model could be used to evaluate management strategies for reducing the impact of brown rot in nectarines during storage.


Assuntos
Frutas , Doenças das Plantas
3.
New Phytol ; 230(4): 1489-1502, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33550584

RESUMO

The trade-off between yield and quality, a major problem for the production of fleshy fruits, involves fruit expansive growth and sugar metabolism. Here we developed an integrative model by coupling a biophysical model of fleshy fruit growth processes, including water and carbon fluxes and organ expansion, with an enzyme-based kinetic model of sugar metabolism to better understand the interactions between these two processes. The integrative model was initially tested on tomato fruit, a model system for fleshy fruit. The integrative model closely simulated the biomass and major carbon metabolites of tomato fruit developing under optimal or stress conditions. The model also performed robustly when simulating the fruit size and sugar concentrations of different tomato genotypes including wild species. The validated model was used to explore ways of uncoupling the size-sweetness trade-off in fruit. Model-based virtual experiments suggested that larger sweeter tomatoes could be obtained by simultaneously manipulating certain biophysical factors and transmembrane transports. The integrative fleshy fruit model provides a promising tool to facilitate the targeted bioengineering and breeding of tomatoes and other fruits.


Assuntos
Solanum lycopersicum , Metabolismo dos Carboidratos , Carbono , Frutas , Melhoramento Vegetal
4.
J Exp Bot ; 71(12): 3463-3474, 2020 06 22.
Artigo em Inglês | MEDLINE | ID: mdl-32420599

RESUMO

We develop a model based on the biophysical representation of water and sugar flows between the pedicel, fruit xylem and phloem, and the fruit apoplast and symplast in order to identify diurnal patterns of transport in the pedicel-fruit system of peach. The model predicts that during the night water is mainly imported to the fruit through the xylem, and that fruit phloem-xylem transfer of water allows sugar concentrations in the phloem to be higher in the fruit than in the pedicel. This results in relatively high sugar transport to the fruit apoplast, leading to relatively high sugar uptake by the fruit symplast despite low sugar concentrations in the pedicel. At midday, the model predicts a xylem backflow of water driven by a lower pressure potential in the xylem than in the fruit apoplast. In addition, fruit xylem-to-phloem transfer of water decreases the fruit phloem sugar concentration, resulting in moderate sugar uptake by the fruit symplast, despite the high sugar concentration in the pedicel. Globally, the predicted fruit xylem-phloem water transfers buffer the sugar concentrations in the fruit phloem and apoplast, leading to a diurnally regulated uptake of sugar. A possible fruit xylem-to-apoplast recirculation of water through the fruit phloem reduces water lost by xylem backflow at midday.


Assuntos
Frutas , Prunus persica , Floema , Açúcares , Água , Xilema
5.
J Exp Bot ; 71(16): 5010-5026, 2020 08 06.
Artigo em Inglês | MEDLINE | ID: mdl-32472678

RESUMO

Although fleshy fruit is mainly made up of water, little is known about the impact of its water status on sugar metabolism and its composition. In order to verify whether fruit water status is an important driver of carbohydrate composition in tomato fruit, an adaptation of the SUGAR model proposed previously by M. Génard and M. Souty was used. Two versions of the model, with or without integrating the influence of fruit water content on carbohydrate metabolism, were proposed and then assessed with the data sets from two genotypes, Levovil and Cervil, grown under different conditions. The results showed that, for both genotypes, soluble sugars and starch were better fitted by the model when the effects of water content on carbohydrate metabolism were taken into consideration. Water content might play a regulatory role in the carbon metabolism from sugars to compounds other than sugars and starch in Cervil fruit, and from sugars to starch in Levovil fruit. While water content influences tomato fruit carbohydrate concentrations by both metabolism and dilution/dehydration effects in the early developmental stage, it is mainly by dilution/dehydration effects in the late stage. The possible mechanisms underlying the effect of the fruit water content on carbohydrate metabolism are also discussed.


Assuntos
Solanum lycopersicum , Metabolismo dos Carboidratos , Carboidratos , Frutas , Solanum lycopersicum/genética , Água
6.
J Exp Bot ; 71(18): 5521-5537, 2020 09 19.
Artigo em Inglês | MEDLINE | ID: mdl-32556164

RESUMO

The cuticle is composed of cutin and cuticular waxes, and it is the first protective barrier to abiotic and biotic stresses in fruit. In this study, we analysed the composition of and changes in cuticular waxes during fruit development in nectarine (Prunus persica L. Batsch) cultivars, in parallel with their conductance and their susceptibility to Monilinia laxa. The nectarine waxes were composed of triterpenoids, mostly ursolic and oleanolic acids, phytosterols, and very-long-chain aliphatics. In addition, we detected phenolic compounds that were esterified with sugars or with triterpenoids, which are newly described in cuticular waxes. We quantified 42 compounds and found that they changed markedly during fruit development, with an intense accumulation of triterpenoids during initial fruit growth followed by their decrease at the end of endocarp lignification and a final increase in very-long-chain alkanes and hydroxylated triterpenoids until maturity. The surface conductance and susceptibility to Monilinia decreased sharply at the beginning of endocarp lignification, suggesting that triterpenoid deposition could play a major role in regulating fruit permeability and susceptibility to brown rot. Our results provide new insights into the composition of cuticular waxes of nectarines and their changes during fruit development, opening new avenues of research to explore brown rot resistance factors in stone fruit.


Assuntos
Ascomicetos , Prunus persica , Frutas , Ceras
7.
J Exp Bot ; 71(19): 5823-5836, 2020 10 07.
Artigo em Inglês | MEDLINE | ID: mdl-32592486

RESUMO

Fleshy fruits are very varied, whether in terms of their composition, physiology, or rate and duration of growth. To understand the mechanisms that link metabolism to phenotypes, which would help the targeting of breeding strategies, we compared eight fleshy fruit species during development and ripening. Three herbaceous (eggplant, pepper, and cucumber), three tree (apple, peach, and clementine) and two vine (kiwifruit and grape) species were selected for their diversity. Fruit fresh weight and biomass composition, including the major soluble and insoluble components, were determined throughout fruit development and ripening. Best-fitting models of fruit weight were used to estimate relative growth rate (RGR), which was significantly correlated with several biomass components, especially protein content (R=84), stearate (R=0.72), palmitate (R=0.72), and lignocerate (R=0.68). The strong link between biomass composition and RGR was further evidenced by generalized linear models that predicted RGR with R-values exceeding 0.9. Comparison of the fruit also showed that climacteric fruit (apple, peach, kiwifruit) contained more non-cellulosic cell-wall glucose and fucose, and more starch, than non-climacteric fruit. The rate of starch net accumulation was also higher in climacteric fruit. These results suggest that the way biomass is constructed has a major influence on performance, especially growth rate.


Assuntos
Actinidia , Climatério , Biomassa , Etilenos , Frutas , Melhoramento Vegetal
8.
PLoS Comput Biol ; 15(6): e1007121, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31220080

RESUMO

The growth of plant organs is a complex process powered by osmosis that attracts water inside the cells; this influx induces simultaneously an elastic extension of the walls and pressure in the cells, called turgor pressure; above a threshold, the walls yield and the cells grow. Based on Lockhart's seminal work, various models of plant morphogenesis have been proposed, either for single cells, or focusing on the wall mechanical properties. However, the synergistic coupling of fluxes and wall mechanics has not yet been fully addressed in a multicellular model. This work lays the foundations of such a model, by simplifying as much as possible each process and putting emphasis on the coupling itself. Its emergent properties are rich and can help to understand plant morphogenesis. In particular, we show that the model can display a new type of lateral inhibitory mechanism that amplifies growth heterogeneities due e.g to cell wall loosening.


Assuntos
Parede Celular/fisiologia , Modelos Biológicos , Desenvolvimento Vegetal/fisiologia , Água/fisiologia , Biologia Computacional
9.
Ann Bot ; 126(3): 455-470, 2020 08 13.
Artigo em Inglês | MEDLINE | ID: mdl-32333754

RESUMO

BACKGROUND AND AIMS: Sugar concentration is a key determinant of fruit quality. Soluble sugars and starch concentrations in fruits vary greatly from one species to another. The aim of this study was to investigate similarities and differences in sugar accumulation strategies across ten contrasting fruit species using a modelling approach. METHODS: We developed a coarse-grained model of primary metabolism based on the description of the main metabolic and hydraulic processes (synthesis of compounds other than sugar and starch, synthesis and hydrolysis of starch, and water dilution) involved in the accumulation of soluble sugars during fruit development. KEY RESULTS: Statistical analyses based on metabolic rates separated the species into six groups according to the rate of synthesis of compounds other than sugar and starch. Herbaceous species (cucumber, tomato, eggplant, pepper and strawberry) were characterized by a higher synthesis rate than woody species (apple, nectarine, clementine, grape and kiwifruit). Inspection of the dynamics of the processes involved in sugar accumulation revealed that net sugar importation, metabolism and dilution processes were remarkably synchronous in most herbaceous plants, whereas in kiwifruit, apple and nectarine, processes related to starch metabolism were temporally separated from other processes. Strawberry, clementine and grape showed a distinct dynamic compared with all other species. CONCLUSIONS: Overall, these results provide fresh insights into species-specific regulatory strategies and into the role of starch metabolism in the accumulation of soluble sugars in fleshy fruits. In particular, inter-specific differences in development period shape the co-ordination of metabolic processes and affect priorities for carbon allocation across species. The six metabolic groups identified by our analysis do not show a clear separation into climacteric and non-climacteric species, possibly suggesting that the metabolic processes related to sugar concentration are not greatly affected by ethylene-associated events.


Assuntos
Actinidia , Solanum lycopersicum , Metabolismo dos Carboidratos , Frutas , Açúcares
10.
Plant J ; 94(4): 685-698, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-29543354

RESUMO

The concentrations of sugars in fruit vary with fruit development, environment and genotype. In general, there were weak correlations between the variations in sugar concentrations and the activities of enzymes directly related with the synthesis or degradation of sugars. This finding suggests that the relationships between enzyme activities and metabolites are often non-linear and are difficult to assess. To simulate the concentrations of sucrose, glucose, fructose and sorbitol during the development of peach fruit, a kinetic model of sugar metabolism was developed by taking advantage of recent profiling data. Cell compartmentation (cytosol and vacuole) was described explicitly, and data-driven enzyme activities were used to parameterize equations. The model correctly accounts for both annual and genotypic variations, which were observed in 10 genotypes derived from an interspecific cross. They provided important information on the mechanisms underlying the specification of phenotypic differences. In particular, the model supports the hypothesis that a difference in fructokinase affinity could be responsible for a low fructose-to-glucose ratio phenotype, which was observed in the studied population.


Assuntos
Frutose/metabolismo , Glucose/metabolismo , Prunus persica/metabolismo , Frutas/genética , Frutas/metabolismo , Genótipo , Cinética , Modelos Biológicos , Fenótipo , Prunus persica/genética , Prunus persica/crescimento & desenvolvimento , Sorbitol/metabolismo , Sacarose/metabolismo , Açúcares/metabolismo
11.
J Exp Bot ; 70(21): 6215-6228, 2019 11 18.
Artigo em Inglês | MEDLINE | ID: mdl-31504751

RESUMO

The development of a new organ is the result of coordinated events of cell division and expansion, in strong interaction with each other. This study presents a dynamic model of tomato fruit development that includes cell division, endoreduplication, and expansion processes. The model is used to investigate the potential interactions among these developmental processes within the context of the neo-cellular theory. In particular, different control schemes (either cell-autonomous or organ-controlled) are tested and compared to experimental data from two contrasting genotypes. The model shows that a pure cell-autonomous control fails to reproduce the observed cell-size distribution, and that an organ-wide control is required in order to get realistic cell-size variations. The model also supports the role of endoreduplication as an important determinant of the final cell size and suggests that a direct effect of endoreduplication on cell expansion is needed in order to obtain a significant correlation between size and ploidy, as observed in real data.


Assuntos
Tamanho Celular , Simulação por Computador , Frutas/citologia , Modelos Biológicos , Ploidias , Solanum lycopersicum/citologia , Proliferação de Células , Endorreduplicação/genética , Genótipo , Solanum lycopersicum/genética , Especificidade de Órgãos , Análise de Componente Principal
12.
J Exp Bot ; 70(9): 2505-2521, 2019 04 29.
Artigo em Inglês | MEDLINE | ID: mdl-30357362

RESUMO

The growth of fleshy fruits is still poorly understood as a result of the complex integration of water and solute fluxes, cell structural properties, and the regulation of whole plant source-sink relationships. To unravel the contribution of these processes to berry growth, a biophysical grape (Vitis vinifera L.) berry growth module was developed and integrated with a whole-plant functional-structural model, and was calibrated on two varieties, Cabernet Sauvignon and Sangiovese. The model captured well the variations in growth and sugar accumulation caused by environmental conditions, changes in leaf-to-fruit ratio, plant water status, and varietal differences, with obvious future application in predicting yield and maturity under a variety of production contexts and regional climates. Our analyses illustrated that grapevines strive to maintain proper ripening by partially compensating for a reduced source-sink ratio, and that under drought an enhanced berry sucrose uptake capacity can reverse berry shrinkage. Sensitivity analysis highlighted the importance of phloem hydraulic conductance, sugar uptake, and surface transpiration on growth, while suggesting that cell wall extensibility and the turgor threshold for cell expansion had minor effects. This study demonstrates that this integrated model is a useful tool in understanding the integration and relative importance of different processes in driving fleshy fruit growth.


Assuntos
Carbono/metabolismo , Vitis/metabolismo , Água/metabolismo , Frutas/crescimento & desenvolvimento , Frutas/metabolismo , Modelos Biológicos , Pressão Osmótica/fisiologia , Floema/crescimento & desenvolvimento , Floema/metabolismo , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Vitis/crescimento & desenvolvimento
13.
J Environ Manage ; 236: 1-16, 2019 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-30710877

RESUMO

In this study, we analyzed the patterns of relationships between multiple ecosystem services in apple orchards by considering the cascade that links agricultural practices to ecosystem functions and then to ecosystem services. Five major ecosystem services were considered: fruit production, soil nitrogen availability, climate regulation, water cycle maintenance and regulation, including water quality, and pest and disease control. We derived indicators of ecosystem functions and of ecosystem services from model simulations of orchards driven by virtual cropping systems combining various modalities of nitrogen fertilization, irrigation, and pest control. We deciphered the links between practices and ecosystem functions and between those functions and ecosystem services and clustered cropping systems according to their ecosystem service supply. Noticeable synergies were found between yield, fruit mass and sequestrated carbon. The contribution of carbon allocation to fruit in sequestrated carbon was considerable. Nitrogen absorption, impacted by fertilization and irrigation, was a major driver of these relationships. The typology built from these virtual cropping systems clearly followed a gradient of provisioning and regulating ecosystem services. Five cropping systems optimized the compromise between provisioning and regulating services and were essentially characterized by organo-mineral fertilization, comfort irrigation, apple scab-resistant cultivars and exclusion nets against codling moth. Our approach could contribute to the design of cropping systems that would provide an acceptable compromise between multiple ecosystem services in orchards.


Assuntos
Ecossistema , Malus , Agricultura , Frutas , Solo
14.
Ann Bot ; 122(1): 1-21, 2018 06 28.
Artigo em Inglês | MEDLINE | ID: mdl-29718072

RESUMO

Background: One of the key goals of fruit biology is to understand the factors that influence fruit growth and quality, ultimately with a view to manipulating them for improvement of fruit traits. Scope: Primary metabolism, which is not only essential for growth but is also a major component of fruit quality, is an obvious target for improvement. However, metabolism is a moving target that undergoes marked changes throughout fruit growth and ripening. Conclusions: Agricultural practice and breeding have successfully improved fruit metabolic traits, but both face the complexity of the interplay between development, metabolism and the environment. Thus, more fundamental knowledge is needed to identify further strategies for the manipulation of fruit metabolism. Nearly two decades of post-genomics approaches involving transcriptomics, proteomics and/or metabolomics have generated a lot of information about the behaviour of fruit metabolic networks. Today, the emergence of modelling tools is providing the opportunity to turn this information into a mechanistic understanding of fruits, and ultimately to design better fruits. Since high-quality data are a key requirement in modelling, a range of must-have parameters and variables is proposed.


Assuntos
Frutas/metabolismo , Redes e Vias Metabólicas , Modelos Biológicos , Plantas/metabolismo , Agricultura , Produtos Agrícolas , Frutas/genética , Frutas/crescimento & desenvolvimento , Metabolômica , Desenvolvimento Vegetal , Plantas/genética , Proteômica
15.
J Exp Bot ; 67(11): 3419-31, 2016 05.
Artigo em Inglês | MEDLINE | ID: mdl-27117339

RESUMO

Knowledge of the genetic control of sugar metabolism is essential to enhance fruit quality and promote fruit consumption. The sugar content and composition of fruits varies with species, cultivar and stage of development, and is controlled by multiple enzymes. A QTL (quantitative trait locus) study was performed on peach fruit [Prunus persica (L.) Batsch], the model species for Prunus Progeny derived from an interspecific cross between P. persica cultivars and P. davidiana was used. Dynamic QTLs for fresh weight, sugars, acids, and enzyme activities related to sugar metabolism were detected at different stages during fruit development. Changing effects of alleles during fruit growth were observed, including inversions close to maturity. This QTL analysis was supplemented by the identification of genes annotated on the peach genome as enzymes linked to sugar metabolism or sugar transporters. Several cases of co-locations between annotated genes, QTLs for enzyme activities and QTLs controlling metabolite concentrations were observed and discussed. These co-locations raise hypotheses regarding the functional regulation of sugar metabolism and pave the way for further analyses to enable the identification of the underlying genes. In conclusion, we identified the potential impact on fruit breeding of the modification of QTL effect close to maturity.


Assuntos
Metabolismo dos Carboidratos/genética , Prunus persica/genética , Cruzamentos Genéticos , Frutas/enzimologia , Frutas/genética , Frutas/crescimento & desenvolvimento , Prunus persica/enzimologia , Prunus persica/crescimento & desenvolvimento , Locos de Características Quantitativas
16.
Ann Bot ; 118(2): 317-30, 2016 08.
Artigo em Inglês | MEDLINE | ID: mdl-27279576

RESUMO

BACKGROUND AND AIMS: Plant growth depends on carbon availability and allocation among organs. QualiTree has been designed to simulate carbon allocation and partitioning in the peach tree (Prunus persica), whereas MappleT is dedicated to the simulation of apple tree (Malus × domestica) architecture. The objective of this study was to couple both models and adapt QualiTree to apple trees to simulate organ growth traits and their within-tree variability. METHODS: MappleT was used to generate architectures corresponding to the 'Fuji' cultivar, accounting for the variability within and among individuals. These architectures were input into QualiTree to simulate shoot and fruit growth during a growth cycle. We modified QualiTree to account for the observed shoot polymorphism in apple trees, i.e. different classes (long, medium and short) that were characterized by different growth function parameters. Model outputs were compared with observed 3D tree geometries, considering shoot and final fruit size and growth dynamics. KEY RESULTS: The modelling approach connecting MappleT and QualiTree was appropriate to the simulation of growth and architectural characteristics at the tree scale (plant leaf area, shoot number and types, fruit weight at harvest). At the shoot scale, mean fruit weight and its variability within trees was accurately simulated, whereas the model tended to overestimate individual shoot leaf area and underestimate its variability for each shoot type. Varying the parameter related to the intensity of carbon exchange between shoots revealed that behaviour intermediate between shoot autonomy and a common assimilate pool was required to properly simulate within-tree fruit growth variability. Moreover, the model correctly dealt with the crop load effect on organ growth. CONCLUSIONS: This study provides understanding of the integration of shoot ontogenetic properties, carbon supply and transport between entities for simulating organ growth in trees. Further improvements regarding the integration of retroaction loops between carbon allocation and the resulting plant architecture are expected to allow multi-year simulations.


Assuntos
Carbono/metabolismo , Malus , Modelos Biológicos , Transporte Biológico , Metabolismo dos Carboidratos , Simulação por Computador , Frutas/anatomia & histologia , Frutas/crescimento & desenvolvimento , Frutas/metabolismo , Malus/anatomia & histologia , Malus/crescimento & desenvolvimento , Malus/metabolismo , Folhas de Planta/anatomia & histologia , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Brotos de Planta/anatomia & histologia , Brotos de Planta/crescimento & desenvolvimento , Brotos de Planta/metabolismo , Árvores
17.
Plant Physiol ; 164(3): 1204-21, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24474652

RESUMO

To assess the influence of the environment on fruit metabolism, tomato (Solanum lycopersicum 'Moneymaker') plants were grown under contrasting conditions (optimal for commercial, water limited, or shaded production) and locations. Samples were harvested at nine stages of development, and 36 enzyme activities of central metabolism were measured as well as protein, starch, and major metabolites, such as hexoses, sucrose, organic acids, and amino acids. The most remarkable result was the high reproducibility of enzyme activities throughout development, irrespective of conditions or location. Hierarchical clustering of enzyme activities also revealed tight relationships between metabolic pathways and phases of development. Thus, cell division was characterized by high activities of fructokinase, glucokinase, pyruvate kinase, and tricarboxylic acid cycle enzymes, indicating ATP production as a priority, whereas cell expansion was characterized by enzymes involved in the lower part of glycolysis, suggesting a metabolic reprogramming to anaplerosis. As expected, enzymes involved in the accumulation of sugars, citrate, and glutamate were strongly increased during ripening. However, a group of enzymes involved in ATP production, which is probably fueled by starch degradation, was also increased. Metabolites levels seemed more sensitive than enzymes to the environment, although such differences tended to decrease at ripening. The integration of enzyme and metabolite data obtained under contrasting growth conditions using principal component analysis suggests that, with the exceptions of alanine amino transferase and glutamate and malate dehydrogenase and malate, there are no links between single enzyme activities and metabolite time courses or levels.


Assuntos
Meio Ambiente , Frutas/enzimologia , Frutas/crescimento & desenvolvimento , Metaboloma , Solanum lycopersicum/enzimologia , Solanum lycopersicum/crescimento & desenvolvimento , Carboxiliases/metabolismo , Análise por Conglomerados , Frutoquinases/metabolismo , Frutas/metabolismo , Hexoses/metabolismo , Solanum lycopersicum/metabolismo , Solanum lycopersicum/fisiologia , Tamanho do Órgão , Proteínas de Plantas/metabolismo , Análise de Componente Principal , Reprodutibilidade dos Testes , Amido/metabolismo , Fatores de Tempo , Vacúolos/metabolismo , Água
18.
J Exp Bot ; 66(11): 3391-404, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25873655

RESUMO

A detailed study of the diurnal compositional changes was performed in tomato (Solanum lycopersicum cv. Moneymaker) leaves and fruits. Plants were cultivated in a commercial greenhouse under two growth conditions: control and shaded. Expanding fruits and the closest mature leaves were harvested during two different day/night cycles (cloudy or sunny day). High-throughput robotized biochemical phenotyping of major compounds, as well as proton nuclear magnetic resonance and mass spectrometry metabolomic profiling, were used to measure the contents of about 70 metabolites in the leaves and 60 metabolites in the fruits, in parallel with ecophysiological measurements. Metabolite data were processed using multivariate, univariate, or clustering analyses and correlation networks. The shaded carbon-limited plants adjusted their leaf area, decreased their sink carbon demand and showed subtle compositional modifications. For source leaves, several metabolites varied along a diel cycle, including those directly linked to photosynthesis and photorespiration. These metabolites peaked at midday in both conditions and diel cycles as expected. However, transitory carbon storage was limited in tomato leaves. In fruits, fewer metabolites showed diel fluctuations, which were also of lower amplitude. Several organic acids were among the fluctuating metabolites. Diel patterns observed in leaves and especially in fruits differed between the cloudy and sunny days, and between the two conditions. Relationships between compositional changes in leaves and fruits are in agreement with the fact that several metabolic processes of the fruit appeared linked to its momentary supply of sucrose.


Assuntos
Carbono/metabolismo , Frutas/metabolismo , Metabolômica , Solanum lycopersicum/metabolismo , Sequestro de Carbono , Ritmo Circadiano , Fotossíntese , Folhas de Planta/metabolismo , Sacarose/metabolismo
19.
J Sci Food Agric ; 95(5): 1055-65, 2015 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-24948582

RESUMO

BACKGROUND: The literature abounds with the impacts of drought conditions on the concentration of non-structural compounds (NSC) in peach fruits without distinction as to the direct effect of drought on fruit metabolism and its indirect effect through dilution. Moreover, there is a need to investigate the sensitivity of the fruit composition to progressive water deficit in semi-arid conditions, as well as the origin of variations in fruit composition - not only in carbohydrates and organic acids, but also in secondary metabolites such as polyphenols. RESULTS: The increase in stress intensity resulted in smaller fruits and a reduction in yield. Drought increased fruit dry matter content, structural dry matter (SDM) content and firmness due to lower water import to fruits, although drought reduced fruit surface conductance and its transpiration. Drought significantly affected the concentrations of each NSC either through the decrease in dilution and/or modifications of their metabolism. The increase in hexoses and sorbitol concentrations of fruits grown under drought conditions resulted in an increase in the sweetness index but not near harvest. Malic acid concentration and content:SDM ratio increased as drought intensified, whereas those of citric and quinic acids decreased. Polyphenol concentration and content increased under severe drought. CONCLUSION: The increase in stress intensity strongly affected fruit mass. The concentration of total carbohydrates and organic acid at harvest increased mainly through a decrease in fruit dilution, whereas the concentrations of polyphenols were also strongly affected through an impact on their metabolism.


Assuntos
Produtos Agrícolas/crescimento & desenvolvimento , Secas , Qualidade dos Alimentos , Frutas/crescimento & desenvolvimento , Polifenóis/biossíntese , Prunus persica/crescimento & desenvolvimento , Estresse Fisiológico , Irrigação Agrícola , Algoritmos , Fenômenos Químicos , Ácido Cítrico/análise , Ácido Cítrico/metabolismo , Produtos Agrícolas/química , Produtos Agrícolas/metabolismo , Carboidratos da Dieta/análise , Frutas/química , Frutas/metabolismo , Frutas/normas , Hexoses/análise , Hexoses/biossíntese , Irã (Geográfico) , Malatos/análise , Malatos/metabolismo , Fenômenos Mecânicos , Polifenóis/análise , Prunus persica/química , Prunus persica/metabolismo , Ácido Quínico/análise , Ácido Quínico/metabolismo , Estações do Ano , Sorbitol/análise , Sorbitol/metabolismo , Propriedades de Superfície
20.
BMC Plant Biol ; 14: 310, 2014 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-25403512

RESUMO

BACKGROUND: Malate is one of the most important organic acids in many fruits and its concentration plays a critical role in organoleptic properties. Several studies suggest that malate accumulation in fruit cells is controlled at the level of vacuolar storage. However, the regulation of vacuolar malate storage throughout fruit development, and the origins of the phenotypic variability of the malate concentration within fruit species remain to be clarified. In the present study, we adapted the mechanistic model of vacuolar storage proposed by Lobit et al. in order to study the accumulation of malate in pre and postharvest fruits. The main adaptation concerned the variation of the free energy of ATP hydrolysis during fruit development. Banana fruit was taken as a reference because it has the particularity of having separate growth and post-harvest ripening stages, during which malate concentration undergoes substantial changes. Moreover, the concentration of malate in banana pulp varies greatly among cultivars which make possible to use the model as a tool to analyze the genotypic variability. The model was calibrated and validated using data sets from three cultivars with contrasting malate accumulation, grown under different fruit loads and potassium supplies, and harvested at different stages. RESULTS: The model predicted the pre and post-harvest dynamics of malate concentration with fairly good accuracy for the three cultivars (mean RRMSE = 0.25-0.42). The sensitivity of the model to parameters and input variables was analyzed. According to the model, vacuolar composition, in particular potassium and organic acid concentrations, had an important effect on malate accumulation. The model suggested that rising temperatures depressed malate accumulation. The model also helped distinguish differences in malate concentration among the three cultivars and between the pre and post-harvest stages by highlighting the probable importance of proton pump activity and particularly of the free energy of ATP hydrolysis and vacuolar pH. CONCLUSIONS: This model appears to be an interesting tool to study malate accumulation in pre and postharvest fruits and to get insights into the ecophysiological determinants of fruit acidity, and thus may be useful for fruit quality improvement.


Assuntos
Frutas/química , Frutas/metabolismo , Malatos/metabolismo , Modelos Biológicos , Vacúolos/metabolismo , Trifosfato de Adenosina/metabolismo , Concentração de Íons de Hidrogênio , Musa/química , Musa/crescimento & desenvolvimento , Musa/metabolismo
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