Effects of the water-soluble fraction of the mixture fuel oil/diesel on the microalgae Dunaliella tertiolecta through growth.

There is evidence that water-soluble fraction (WSF) from fuel oil/diesel mixture affects marine microbiota. In order to establish a sequence of WSF effects during microalgal growth, this work aimed to monitor Dunaliella tertiolecta exposed to WSF during 15 days. Three different pigments (chlorophyll a, lutein, and ß-carotene) and four metabolites (protein, lipids, fatty acids, and phenols) were studied, and FTIR spectroscopy was used to determine the biomolecular transitions of lipids and their accumulation. The results show that D. tertiolecta triggered a physiological and biochemical response with changes in growth rate, pigments, phenols, lipids, and proteins of the microalga, although fatty acid profile was unaltered. For all the biochemical parameters altered, there were significant differences with the controls. At the end of the assay, exposed D. tertiolecta showed similar values with the control on all the compounds analyzed, except lipids. FTIR absorbance showed an increase in unsaturated acyl chains within the exposed microalgae, giving support for a possible uptake of hydrocarbons from WSF. Variation in pigments and phenol contents is presented as an integrated antioxidant response to the stress imposed by WSF. Overall, this research provides information about the effects of WSF on D. tertiolecta, and the ability of this microalga to recover after long-term exposure to the water-soluble fraction of fuel oil/diesel.

Study of the morphology of ZnS thin films deposited on different substrates via chemical bath deposition.

In this work, the influence of substrate on the morphology of ZnS thin films by chemical bath deposition is studied. The materials used were zinc acetate, tri-sodium citrate, thiourea, and ammonium hydroxide/ammonium chloride solution. The growth of ZnS thin films on different substrates showed a large variation on the surface, presenting a poor growth on SiO2 and HfO2 substrates. The thin films on ITO substrate presented a uniform and compact growth without pinholes. The optical properties showed a transmittance of about 85% in the visible range of 300-800 nm with band gap of 3.7 eV.

Solvothermal synthesis of nickel-tungsten sulfides for 2-propanol dehydration.

The bimetallic nickel-tungsten catalysts were prepared via solvothermal method. The X-ray Diffractometer (XRD) analysis revealed that the corresponding peaks at 14°, 34°, and 58° were for tungsten disulfide (WS2 ) hexagonal phase. The catalysts displayed different crystalline phase with nickel addition, and as an effect the WS2 surface area decreased from 74.7 to 2.0 m(2) g(--1) . In this sense, high-resolution transmission electron microscopy (HRTEM) showed the layers set in direction (002) with an onion-like morphology, and in the center of the particles there is a large amount of nickel contained with 6-8 layers covering it. The catalytic dehydration of 2-propanol was selective to propene in 100% at 250 °C for the sample with 0.7 of atomic ratio of Ni/Ni + W.

Effect of polycyclic aromatic hydrocarbons on the pallial fluid buffering capacity of the marine mussel, Mytilus galloprovincialis.

Pallial fluid buffering capacity of the sea mussel Mytilus galloprovincialis was investigated to establish the potential of this biological parameter to serve as a biomarker. Four groups of 15 animals were used in a 72-h toxicity test. Group 1, the aerobic control group, was placed in a filtered aerated natural seawater aquarium. Groups 2-4 were subjected to hypoxic conditions by removal from water after animals were injected with a single dose of the following: group 2 (anaerobic control) was administered 10 microl of UV-treated filtered natural seawater; group 3 (anaerobic solvent control) was injected with 10 microl of acetonitrile and group 4 (PAH exposed group) with 10 microl of 2 mM anthracene. Pallial fluid was taken from all the animals following seawater immersion or air exposure. Pallial fluid from each individual was extracted, adjusted to pH 5.0 and titrated with NaOH until reaching pH 6.0. The buffering capacity index (beta), defined as the amount of mu equivalents of NaOH needed to change in one unit the pH of a 5-ml sample of pallial fluid, was calculated for each group. Values were: for group 1, beta = 3.17 (+/- 0.782); for group 2, beta=15.713 (+/-2.992); group 3 was beta=18.124 (+/-2.288); and group 4 was beta=28.109 (+/-11.398). The statistically significant increase (P<0.05) in group 4 compared with the other groups indicates that the buffering capacity index (beta) is a worthy biological parameter to be further explored as a biomarker for ecotoxicological monitoring programs. The increase in buffering capacity is discussed and a biochemical link between anaerobic metabolism and the exposure to PAH is suggested to explain changes of this biological parameter.