Use of distributed acoustic sensing for Doppler tracking of moving sources.

Distributed acoustic sensing (DAS) via fiber-optic reflectometry techniques is finding more and more applications in recent years. In many of these applications, the position of detected acoustic or seismic sources is defined with a single longitudinal coordinate which specifies the distance between the detection point in the fiber to the DAS interrogator. In this paper we describe a DAS system which is intended to operate in a fluid (air or water) and to detect and localize moving objects, with three spatial coordinates, using the acoustic waves they generate or reflect and their Doppler shifts. The new method uses optical frequency domain reflectometry (OFDR) and lumped Rayleigh reflectors (LRR's) to ensure sufficiently high sensitivity for operation in fluid media. The new method was used to track a narrowband (CW) signal source.

Lumped Rayleigh reflectors.

Distributed acoustic sensing (DAS) via optical fibers makes use of Rayleigh backscattering for the detection of acoustic waves that interact with the fiber along its entire length. The random nature of Rayleigh backscattering leads to nonuniform performance along the fiber and, occasionally, to complete signal fading. In addition, distance-dependent signal-to-noise (SNR) degradation is always present due to propagation loss. In contrast, using arrays of discrete reflectors [such as weak fiber Bragg gratings (FBGs) with equal center wavelengths] offers deterministic performance which can be designed to be uniform along the fiber. Here we describe an approach for implementing Rayleigh-based discrete reflectors that can offer enhanced detection performance in selected regions. It is based on enclosing sections of the fiber in acoustically insulated boxes to create lumped Rayleigh reflectors. Besides diminishing the randomness in detection sensitivity, the method enables increasing the detection SNR far beyond the typical value for Rayleigh-based DAS and obtaining sensitivities comparable with discrete reflectors. The proposed method was successfully tested via both simulation and experiment.

On the sensitivity of distributed acoustic sensing.

In distributed acoustic sensing (DAS) an optical fiber is transformed into an array of thousands of "virtual microphones." Most current DAS methodologies are based on coherent interference of Rayleigh backscattered light and thus are prone to signal fading. Hence, the sensitivities of the "microphones" fluctuate randomly along the fiber. Therefore, specifying the sensitivity of DAS without considering its random nature is incomplete and of limited value. In this Letter, the statistical properties of DAS SNR and DAS sensitivity are studied in detail for the first time, to the best of our knowledge. It is shown that the mean dynamic DAS SNR is proportional to the SNR obtained in a single measurement of the fiber's "static" backscatter profile and, in turn, to the energy of the interrogation pulse. Finally, the minimum input signal, which produces a specified mean DAS SNR, is proposed as a new figure of merit for the characterization of system performances and for comparison between the sensitivities of different DAS modalities.

Multiplexing of fiber-optic ultrasound sensors via swept frequency interferometry.

The use of fiber-optic sensors for ultrasound (US) detection has many advantages over conventional piezoelectric detectors. However, the issue of multiplexing remains a major challenge. Here, a novel approach for multiplexing fiber-optic based US sensors using swept frequency interferometry is introduced. Light from a coherent swept source propagates in an all-fiber interferometric network made of a reference arm and a parallel connection of N sensing arms. Each sensing arm comprises a short polyimide coated sensing section (~4cm), which is exposed to the US excitation, preceded by a delay of different length. When the instantaneous frequency of the laser is linearly swept, the receiver output contains N harmonic beat components which correspond to the various optical paths. Exposing the sensing sections to US excitation introduces phase modulation of the harmonic components. The US-induced signals can be separated in the frequency domain and be extracted from their carriers by common demodulation techniques. The method was demonstrated by multiplexing 4 sensing fibers and detecting microsecond US pulses which were generated by a 2.25MHz ultrasound transducer. The pulses were successfully measured by all sensing fibers without noticeable cross-talk.

Optical frequency domain reflectometry at maximum update rate using I/Q detection.

We introduce a new optical frequency domain reflectometry (OFDR) system and processing method that utilize negative beat frequencies for the first time. The new approach enables efficient use of the available system bandwidth and facilitates distributed sensing with the maximum allowable update rate for a given fiber length. This is achieved by using a coherent optical-communications-type receiver that detects both the in-phase (I) and quadrature (Q) components of the backscatter field. The I and Q components are digitally combined to produce a complex backscatter signal whose Fourier transform is not necessarily symmetric. Judicious processing of the complex backscatter signal maps the reflection profile of one half of the sensing fiber to positive beat-frequencies and the profile of the other half to negative beat-frequencies. The new approach was tested via comprehensive computer simulations and experiment.

Continuous wide-field characterization of drug release from skin substitute using off-axis interferometry.

We achieved continuous, noncontact wide-field imaging and characterization of drug release from a polymeric device in vitro by uniquely using off-axis interferometric imaging. Unlike the current gold-standard methods in this field, which are usually based on chromatography and spectroscopy, our method requires no user intervention during the experiment and involves less lab consumable instruments. Using a simplified interferometric imaging system, we experimentally demonstrate the characterization of anesthetic drug release (Bupivacaine) from a soy-based protein matrix, which is used as a skin substitute for wound dressing. Our results demonstrate the potential of interferometric imaging as an inexpensive and easy-to-use alternative for characterization of drug release in vitro.

Dual-channel low-coherence interferometry and its application to quantitative phase imaging of fingerprints.

We introduce an off-axis, wide-field, low-coherence and dual-channel interferometric imaging system, which is based on a simple-to-align, common-path interferometer. The system requires no optical-path-difference matching between the interferometric arms in order to obtain interference with low-coherence light source, and is capable of achieving two channels of off-axis interference with high spatial frequency. The two 180°-phase-shifted interferograms are acquired simultaneously using a single digital camera, and processed into a single, noise-reduced and DC-suppressed interferogram. We demonstrate using the proposed system for phase imaging of fingerprint templates. Due to the fact that conventional phase unwrapping algorithms cannot handle the complex and deep surface topography imposed by fingerprint templates, we experimentally implemented two-wavelength phase unwrapping using a supercontinuum laser coupled to acousto-optical tunable filter, together functioning as a low-coherence tunable light source. From the unwrapped phase map, we produced high quality depth profiles of fingerprint templates.

Optical-mechanical signatures of cancer cells based on fluctuation profiles measured by interferometry.

We propose to establish a cancer biomarker based on the unique optical-mechanical signatures of cancer cells measured in a noncontact, label-free manner by optical interferometry. Using wide-field interferometric phase microscopy (IPM), implemented by a portable, off-axis, common-path and low-coherence interferometric module, we quantitatively measured the time-dependent, nanometer-scale optical thickness fluctuation maps of live cells in vitro. We found that cancer cells fluctuate significantly more than healthy cells, and that metastatic cancer cells fluctuate significantly more than primary cancer cells. Atomic force microscopy (AFM) measurements validated the results. Our study shows the potential of IPM as a simple clinical tool for aiding in diagnosis and monitoring of cancer.