Learning to collaborate: bringing together behavior and quantitative genomics.

The genetic basis of complex trait like learning and memory have been well studied over the decades. Through those groundbreaking findings, we now have a better understanding about some of the genes and pathways that are involved in learning and/or memory. However, few of these findings identified the naturally segregating variants that are influencing learning and/or memory within populations. In this special issue honoring the legacy of Troy Zars, we review some of the traditional approaches that have been used to elucidate the genetic basis of learning and/or memory, specifically in fruit flies. We highlight some of his contributions to the field, and specifically describe his vision to bring together behavior and quantitative genomics with the aim of expanding our knowledge of the genetic basis of both learning and memory. Finally, we present some of our recent work in this area using a multiparental population (MPP) as a case study and describe the potential of this approach to advance our understanding of neurogenetics.

Dopaminergic neurons can influence heat-box place learning in Drosophila.

Dopamine provides crucial neuromodulatory functions in several insect and rodent learning and memory paradigms. However, an early study suggested that dopamine may be dispensable for aversive place memory in Drosophila. Here we tested the involvement of particular dopaminergic neurons in place learning and memory. We used the thermogenetic tool Gr28bD to activate protocerebral anterior medial (PAM) cluster and non-PAM dopaminergic neurons in an operant way in heat-box place learning. We show that activation of PAM neurons influences performance during place learning, but not during memory testing. These findings provide a gateway to explore how dopamine influences place learning.

Reinforcement signaling of punishment versus relief in fruit flies.

Painful events establish opponent memories: cues that precede pain are remembered negatively, whereas cues that follow pain, thus coinciding with relief are recalled positively. How do individual reinforcement-signaling neurons contribute to this "timing-dependent valence-reversal?" We addressed this question using an optogenetic approach in the fruit fly. Two types of fly dopaminergic neuron, each comprising just one paired cell, indeed established learned avoidance of odors that preceded their photostimulation during training, and learned approach to odors that followed the photostimulation. This is in striking parallel to punishment versus relief memories reinforced by a real noxious event. For only one of these neuron types, both effects were strong enough for further analyses. Notably, interfering with dopamine biosynthesis in these neurons partially impaired the punishing effect, but not the relieving after-effect of their photostimulation. We discuss how this finding constraints existing computational models of punishment versus relief memories and introduce a new model, which also incorporates findings from mammals. Furthermore, whether using dopaminergic neuron photostimulation or a real noxious event, more prolonged punishment led to stronger relief. This parametric feature of relief may also apply to other animals and may explain particular aspects of related behavioral dysfunction in humans.

Aversive olfactory associative memory loses odor specificity over time.

Avoiding associatively learned predictors of danger is crucial for survival. Aversive memories can, however, become counter-adaptive when they are overly generalized to harmless cues and contexts. In a fruit fly odor-electric shock associative memory paradigm, we found that learned avoidance lost its specificity for the trained odor and became general to novel odors within a day of training. We discuss the possible neural circuit mechanisms of this effect and highlight the parallelism to over-generalization of learned fear behavior after an incubation period in rodents and humans, with due relevance for post-traumatic stress disorder.