PRO-INFLAMMATORY EFFECTS OF EXPERIMENTAL HYPERTHYROIDISM IN COLON OF MICE (IMMUNOHISTOCYTOCHEMICAL STUDY).

Thyroid hormones can exert responses in various immune cells affecting several inflammation-related processes. The interactions between the endocrine and immune systems have been shown to contribute to pathophysiological conditions. Aim of study - to answer the question if hyper- or/and hypothyroid state can be as provoking factor for inflammation in colon. The study was performed on 60 mature male mice of the inbred line C3H-A. On male mice was reproduced a model of experimental hyperthyroidism and hypothyroidism by oral intake of L-thyroxin and propylthyouracil, accordingly. By the 22nd week of the experiment all animals were sacrificed. The rectum was removed for histo- and immunocytochemical examination. In histological examination in hyperthyroid group the hypertrophy of the mucous membrane of the colon is detected. Simultaneously the frequency of cell mitosis is increased. Among the epithelium glands of the colon can clearly be seen apoptotic bodies. Moderate or expressed infiltration by lymphocytes and plasma cells is elucidated in mucous membrane of colon relatively to mucous membrane of colon of euthyroid group i.e. control one. In hyporthyroid group the hyportrophy of the mucous membrane of the colon was detected. The lymphocytic infiltration is not found. In immunocytochemical examination the frequency of CD20 and CD56 positive cells was significantly higher (>23%) in cytological preparation of membrane mucous of colon from hyperthyroid mice (p<0.01). Meanwhile CD20 and CD56 positive cells were detected in single cases in cytological preparation of membrane mucous of colon from hypothyroid mice. The same observation was done for healthy membrane mucous from control (euthyroid) group. It is possible that the thyroid status is one of the factors modulating the inflammation. This discussion is far from over, because it is unclear whether prolonged hyperthyroidism can actually activate pro-inflammatory reactions, which subsequently, in turn, activate carcinogenesis.

UNEQUAL HORMONAL RESPONSE OF MALE AND FEMALE C3H-A MICE TO THE THYROID AND ANTITHYROID AGENT TREATMENTS.

The state of physiological functions of the whole organism, its vital activity and adaptation to various changes in the surrounding and internal environment is controlled by neurohumoral mechanisms. The main place in the implementation of those mechanisms belongs to hormones. A clinically relevant problem is currently the relationship between activity of thyroid gland and prolactin. Aim of study. - To elucidate further the relationship of thyroid-stimulating hormone, thyroxine and prolactin. The study was performed on virgin 30 mature male mice and 33 mature female mice of the inbred line C3H-A. On male and female mice was reproduced a model of experimental hyperthyroid and hypothyroid status by the administration to L-thyroxin and propylthyouracil, accordingly. The blood samples from animals were assayed for TSH, T4 (total) and prolactin. In the hyperthyroid male mice the level of T4 (total) was significantly higher as compared to the hypothyroid and control groups. No deference for the level of TSH and prolactin was found between the hyperthyroid and the hypothyroid groups. In the hypothyroid female mice the level of TSH and T4 (total) was significantly lower and the level of prolactin was significantly higher as compared to the hyperthyroid and the control groups. The male and female mice responded in different ways upon the administration to L-thyroxin and propylthyouracil on the level of TSH, T4 (total) and prolactin.

The incidence of breast tumor during experimental hyperthyroidism.

The incidence of breast tumor in inbred C3H-A mice (virgin female) from hyperthyroid, hypothyroid, and euthyroid control groups at week 44 of the experiment was 86.67, 30, and 62.5%, respectively. The percentage of metastatic lymph nodes in these groups was 65.9±16.7, 35.9±5.9, and 53.9±7.2%, respectively (p<0.005). It was shown that hyperthyroidism was associated with increased incidence and aggressiveness of breast tumor, while hypothyroidism was associated with lower incidence of breast tumor in comparison with the control.

[Clinical and immunological efficiency of sodium nucleospermate in treating chronic endometritis and infertility complicated by HPV infection].

Clinical efficacy of sodium nucleospermate (SNS) has shown in the treatment of 26 female patients with chronic endometritis, unsuccessful attempts at in vitro fertilization, and persistent viral infection. Cells from endometrium were processed by liquid cytology and studied by immunocytochemistry techniques. It has been clearly established that, after SNS treatment, the frequency of CD20- and CD56-positive cells was significantly decreased (p < 0.05) and the frequency of TLR4 and TLR9 expressing cells in endometrium has been significantly improved (p < 0.05). In addition, the human papilloma virus (HPV) load was reduced as manifested by the real-time PCR test. The obtained results show good prospects for successful use SNS as a means of pathogenetic therapy in patients with this diagnosis.

[The time course of experimentally altered thyroid status manifestations in female C3H-A mice].

The female C3H-A mice with agouty fur color were used to model hyper- and hypothyroidism in the long lasting experiment. The study was carried out for 44 weeks. Hyperthyroidism was induced by the administration of the L-thyroxine injections on alternate days during the whole period of the investigation. Hypothyroidism was achieved by adding propylthiouracil to the drinking water. The change of thyroid state was characterized by biphasic change in body weight. At the beginning of the experiment the hypothyroid animals were retarding by their weight. Otherwise the hyperthyroid animals were advancing by their weight. But since the 18th-21st week the initial trends changed, i. e. the hypothyroid mice body weight started ahead the hyperthyroid one. In the open field test both hypo- and hyperthyroid animals demonstrated the higher level of the investigating activity in comparison with the euthyroid mice. In the hyperthyroid mice the frequency of side-activity acts (grooming) increased significantly. Thus, the hyperthyroid animals appeared to be more anxious. To the 18th week of the experiment the animals of study groups started to demonstrate the apparent visual difference in their fur color. The hyperthyroid mice fur color became darker than one of the hypothyroid and the euthyroid mice. It is worthy of note that the hyperthyroid mice fur color was getting lighter than one of the euthyroid animals. The results are discussed in the context of hypothalamic-pituitary-thyroid axis functioning. The possible mechanisms of hormonal regulation of the fur color in mice are considered to include the hypothalamic-pituitary-thyroid axis hormones activities.