RESUMO
Retrotransposition affects genome structure by increasing repetition and producing insertional mutations. Dispersion of the retrotransposon L1 throughout mammalian genomes suggests that L1 activity might be an important evolutionary force. Here we report that L1 retrotransposition contributes to rapid genome evolution in the mouse, because a number of L1 sequences from the T(F) subfamily are retrotransposition competent. We show that the T(F) subfamily is large, young and expanding, containing approximately 4,800 full-length members in strain 129. Eleven randomly isolated, full-length T(F) elements averaged 99.8% sequence identity to each other, and seven of these retrotransposed in cultured cells. Thus, we estimate that the mouse genome contains approximately 3,000 active T(F) elements, 75 times the estimated number of active human L1s. Moreover, as T(F) elements are polymorphic among closely related mice, they have retrotransposed recently, implying rapid amplification of the subfamily to yield genomes with different patterns of interspersed repetition. Our data show that mice and humans differ considerably in the number of active L1s, and probably differ in the contribution of retrotransposition to ongoing sequence evolution.
Assuntos
Evolução Molecular , Amplificação de Genes , Genoma , Regiões 5' não Traduzidas , Animais , Humanos , Camundongos , Dados de Sequência Molecular , Mutação , Polimorfismo Genético , Retroelementos , Especificidade da EspécieRESUMO
Human La protein has been shown to serve as a transcription factor for RNA polymerase III (pol III) by facilitating transcription termination and recycling of transcription complexes. In addition, La binds to the 3' oligo(U) ends common to all nascent pol III transcripts, and in the case of B1-Alu RNA, protects it from 3'-end processing (R. J. Maraia, D. J. Kenan, and J. D. Keene, Mol. Cell. Biol. 14:2147-2158, 1994). Others have previously dissected the La protein into an N-terminal domain that binds RNA and a C-terminal domain that does not. Here, deletion and substitution mutants of La were examined for general RNA binding, RNA 3'-end protection, and transcription factor activity. Although some La mutants altered in a C-terminal basic region bind RNA in mobility shift assays, they are defective in RNA 3'-end protection and do not support transcription, while one C-terminal substitution mutant is defective only in transcription. Moreover, a C-terminal fragment lacking RNA binding activity appears able to support low levels of transcription by pol III. While efficient multiround transcription is supported only by mutants that bind RNA and contain a C-terminal basic region. These analyses indicate that RNA binding contributes to but is not sufficient for La transcription factor activity and that the C-terminal domain plays a role in transcription that is distinguishable from simple RNA binding. The transcription factor activity of La can be reversibly inhibited by RNA, suggesting the potential for feedback inhibition of pol III transcription.
Assuntos
Autoantígenos/metabolismo , RNA Polimerase III/metabolismo , RNA/metabolismo , Ribonucleoproteínas/metabolismo , Fatores de Transcrição/metabolismo , Animais , Autoantígenos/genética , Extratos Celulares , Núcleo Celular , Humanos , Camundongos , Mutação , Ligação Proteica , Processamento Pós-Transcricional do RNA/fisiologia , Proteínas Recombinantes de Fusão , Ribonucleoproteínas/genética , Fatores de Transcrição/genética , Transcrição Gênica/fisiologia , Antígeno SS-BRESUMO
Eukaryotic precursor (pre)-tRNAs are processed at both ends prior to maturation. Pre-tRNAs and other nascent transcripts synthesized by RNA polymerase III are bound at their 3' ends at the sequence motif UUUOH [3' oligo(U)] by the La antigen, a conserved phosphoprotein whose role in RNA processing has been associated previously with 3'-end maturation only. We show that in addition to its role in tRNA 3'-end maturation, human La protein can also modulate 5' processing of pre-tRNAs. Both the La antigen's N-terminal RNA-binding domain and its C-terminal basic region are required for attenuation of pre-tRNA 5' processing. RNA binding and nuclease protection assays with a variety of pre-tRNA substrates and mutant La proteins indicate that 5' protection is a highly selective activity of La. This activity is dependent on 3' oligo(U) in the pre-tRNA for interaction with the N-terminal RNA binding domain of La and interaction of the C-terminal basic region of La with the 5' triphosphate end of nascent pre-tRNA. Phosphorylation of La is known to occur on serine 366, adjacent to the C-terminal basic region. We show that this modification interferes with the La antigen's ability to protect pre-tRNAiMet from 5' processing either by HeLa extract or purified RNase P but that it does not affect interaction with the 3' end of pre-tRNA. These findings provide the first evidence to indicate that tRNA 5'-end maturation may be regulated in eukaryotes. Implications of triphosphate recognition is discussed as is a role for La phosphoprotein in controlling transcriptional and posttranscriptional events in the biogenesis of polymerase III transcripts.
Assuntos
Adenosina Trifosfatases/metabolismo , Autoantígenos/metabolismo , Precursores de RNA/metabolismo , Ribonucleoproteínas/metabolismo , Fatores de Transcrição/metabolismo , Sistema Livre de Células , Endorribonucleases/metabolismo , Células HeLa , Humanos , Fosforilação , Poli U/metabolismo , RNA Bacteriano/metabolismo , RNA Catalítico/metabolismo , RNA de Transferência de Metionina/metabolismo , Ribonuclease P , Antígeno SS-BRESUMO
Two putative group I introns in the nuclear ribosomal RNA genes of Hymenoscyphus ericae are described. One is in the small subunit gene about 30 nucleotides upstream of the 3' end of the gene at a site common to several other group I introns. The other is in the large subunit gene approx. 930 bp downstream of the 5' end of the gene. This is the only report of an intron at this location.
Assuntos
Ascomicetos/genética , Genes Fúngicos , Íntrons , RNA Ribossômico/genética , Sequência de Bases , Dados de Sequência MolecularRESUMO
Transposon-like elements flanked by inverted repeats, although common in invertebrates, have only recently been found in vertebrates. We report the presence of Tc1 transposon-like sequences in salmon, trout and charr species and find that these elements belong to several families that do not follow phylogenetic lines. As many as 15,000 copies reside in the Atlantic salmon haploid genome. The complete DNA sequence of one of these transposon-like elements (SALT1) is 1535 base-pairs long, including 35 nucleotide-long terminal inverted repeats. It contains a degenerate open reading frame (ORF) of 1273 nucleotides whose inferred amino acid sequence shares sequence similarity with the "D,D35E" family of transposases, particularly those from Caenorhabditis sp. and Drosophila sp. Southern blot analysis indicated that Tc1 transposon-like sequences are present in other lower vertebrates, including several fish species and amphibians, but the copy number can vary significantly in different lineages.
Assuntos
Elementos de DNA Transponíveis/genética , Variação Genética/genética , Salmonidae/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Clonagem Molecular , Dados de Sequência Molecular , Filogenia , Sequências Repetitivas de Ácido Nucleico/genética , Alinhamento de Sequência , Análise de Sequência de DNA , Especificidade da EspécieRESUMO
When Atlantic salmon (Salmo salar) genomic DNA is digested with the restriction endonuclease BglI and the fragments separated by agarose-gel electrophoresis, bands corresponding to approximately 430 and 923 bp are visualized after EtdBr staining. The 923-bp band was excised from a preparative gel and used to screen a salmon genomic library for recombinant phage (re-phage) containing the repeat. The BglI repeat element is tandemly arrayed, and an array from one re-phage has been sequenced. The BglI repeats comprise 2.3% of the S. salar genome and have been found in the vicinity of rDNA genes (encoding ribosomal RNA). Southern blot hybridization detects a homologue of the Atlantic salmon BglI repeat in the brown trout (Salmo trutta) genome, but not in other salmonids. However, a DNA fragment with sequence homology to part of the BglI repeat has recently been isolated from Arctic charr (Salvelinus alpinus; S.E. Hartley and W.S.D., unpublished data). In addition, the BglI repeat detects RFLPs in Atlantic salmon.
Assuntos
Sequências Repetitivas de Ácido Nucleico , Salmão/genética , Animais , Sequência de Bases , Clonagem Molecular , DNA Ribossômico , Desoxirribonucleases de Sítio Específico do Tipo II , Marcadores Genéticos , Genoma , Dados de Sequência Molecular , Polimorfismo Genético , Análise de Sequência de DNA , Especificidade da EspécieRESUMO
We present here the sequence and characterization of various minisatellite-like tandem repeat loci isolated from the genome of Atlantic salmon (Salmo salar). Their diversity of sequence and lack of core motifs common to minisatellites of other species suggest the presence of numerous and previously unidentified simple sequence repeat families in this salmonid. Evidence for their ubiquity was provided by screening of a salmon genomic library. Southern blot analysis of the phylogenetic distribution of a subset of the minisatellites found one sequence to be pervasive among vertebrates, others present only in Salmoninae or Salmonidae species, and one amplified only in Atlantic salmon. There is evidence for the positioning of microsatellite and minisatellite arrays in close proximity at many loci. Furthermore, one tandem repeat appears to have been inserted into the transposase coding region of a copy of the Tc1 transposon-like element recently identified in salmonids.
Assuntos
Filogenia , Sequências Repetitivas de Ácido Nucleico , Salmão/genética , Animais , Sequência de Bases , Repetições de Microssatélites , Dados de Sequência MolecularRESUMO
The genomes of the two species in the genus Salmo (Atlantic salmon, S. salar; brown trout, S. trutta) contain a 380-bp repetitive element that is flanked by the recognition sequence of the restriction enzyme NheI. These elements, which comprise approximately 1.2% of the salmon genome, do not exist in long tandem arrays as is typical of satellite DNA. A comparison of the sequences of 16 salmon and 7 trout elements revealed that members of this family of repetitive DNA are closely related to one another (over 95% identity). Subfamily structure exists and there is evidence that members of the same subfamilies are found in both Salmo species. A search of the GenBank database indicated that sequences homologous to the NheI repeat are located within a 1424-bp segment inserted immediately downstream of the 5' end of a Tc1 transposon-like sequence isolated from Atlantic salmon (A.R. Radice, B. Bugaj, D.H. Fitch, and S.W. Emmons, unpublished data; GenBank accession No. L12206).
Assuntos
Sequências Repetitivas de Ácido Nucleico , Salmão/genética , Truta/genética , Animais , Sequência de Bases , Evolução Biológica , Sequência Consenso , DNA/genética , Elementos de DNA Transponíveis , Desoxirribonucleases de Sítio Específico do Tipo II , Genoma , Dados de Sequência Molecular , Família Multigênica , Filogenia , Salmonidae/classificação , Salmonidae/genética , Homologia de Sequência do Ácido Nucleico , Especificidade da EspécieRESUMO
Efficient synthesis of many small abundant RNAs is achieved by the proficient recycling of RNA polymerase (pol) III and stable transcription complexes. Cellular Alu and related retroposons represent unusual pol III genes that are normally repressed but are activated by viral infection and other conditions. The core sequences of these elements contain pol III promoters but must rely on fortuitous downstream oligo(dT) tracts for terminator function. We show that a B1-Alu gene differs markedly from a classical pol III gene (tRNAiMet) in terminator sequence requirements. B1-Alu genes that differ only in terminator sequence context direct differential RNA 3' end formation. These genes are assembled into stable transcription complexes but differ in their ability to be recycled in the presence of the La transcription termination factor. La binds to the nascent RNA 3' UUUOH end motif that is generated by transcriptional termination within the pol III termination signal, oligo(dT). We found that the recycling efficiency of the B1-Alu genes is correlated with the ability of La to access the 3' end of the nascent transcript and protect it from 3'-5' exonucleolytic processing. These results illuminate a relationship between RNA 3' end formation and transcription termination, and La-mediated reinitiation by pol III.
Assuntos
Elementos Alu/genética , Autoantígenos/fisiologia , RNA Polimerase III/metabolismo , RNA/biossíntese , Ribonucleoproteínas/fisiologia , Regiões Terminadoras Genéticas/genética , Fatores de Transcrição/metabolismo , Humanos , Regiões Promotoras Genéticas/genética , RNA de Transferência de Metionina/genética , Proteínas de Ligação a RNA/metabolismo , Transcrição Gênica/genética , Fatores Estimuladores Upstream , Antígeno SS-BRESUMO
Active LINE-1 (L1) elements possess the ability to transduce non-L1 DNA flanking their 3' ends to new genomic locations. Occasionally, the 3' end processing machinery may bypass the L1 polyadenylation signal and instead utilize a second downstream polyadenylation site. To determine the frequency of L1-mediated transduction in the human genome, we selected 66 previously uncharacterized L1 sequences from the GenBank database. Fifteen (23%) of these L1s had transposed flanking DNA with an average transduction length of 207 nucleotides. Since there are approximately 400 000 L1 elements, we estimate that insertion of transduced sequences alone may have enlarged the diploid human genome as much as 19 Mb or 0.6%. We also examined 24 full-length mouse L1s and found two long transduced sequences. Thus, L1 retrotransposition in vivo commonly transduces sequence flanking the 3' end of the element.
Assuntos
Regiões 3' não Traduzidas/genética , Elementos Nucleotídeos Longos e Dispersos/genética , Recombinação Genética , Regiões 5' não Traduzidas/genética , Animais , Composição de Bases/genética , Sequência de Bases , Linhagem Celular , Sequência Consenso , Humanos , Camundongos , Dados de Sequência Molecular , Alinhamento de SequênciaRESUMO
Unlike human L1 retrotransposons, the 5' UTR of mouse L1 elements contains tandem repeats of approximately 200 bp in length called monomers. Multiple L1 subfamilies exist in the mouse which are distinguished by their monomer sequences. We previously described a young subfamily, called the T(F) subfamily, which contains approximately 1800 active elements among its 3000 full-length members. Here we characterize a novel subfamily of mouse L1 elements, G(F), which has unique monomer sequence and unusual patterns of monomer organization. A majority of these G(F) elements also have a unique length polymorphism in ORF1. Polymorphism analysis of G(F) elements in various mouse subspecies and laboratory strains revealed that, like T(F), the G(F) subfamily is young and expanding. About 1500 full-length G(F) elements exist in the diploid mouse genome and, based on the results of a cell culture assay, approximately 400 G(F) elements are potentially capable of retrotransposition. We also tested 14 A-type subfamily elements in the assay and estimate that about 900 active A elements may be present in the mouse genome. Thus, it is now known that there are three large active subfamilies of mouse L1s; T(F), A, and G(F), and that in total approximately 3000 full-length elements are potentially capable of active retrotransposition. This number is in great excess to the number of L1 elements thought to be active in the human genome.
Assuntos
Elementos Nucleotídeos Longos e Dispersos/genética , Regiões 3' não Traduzidas/genética , Regiões 5' não Traduzidas/genética , Animais , Sequência de Bases , Células HeLa , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Dados de Sequência Molecular , Mutagênese Insercional/genética , Fases de Leitura Aberta/genética , Filogenia , Polimorfismo Genético/genética , Células Tumorais CultivadasRESUMO
The human La antigen is an RNA-binding protein that facilitates transcriptional termination and reinitiation by RNA polymerase III. Native La protein fractionates into transcriptionally active and inactive forms that are unphosphorylated and phosphorylated at serine 366, respectively, as determined by enzymatic and mass spectrometric analyses. Serine 366 comprises a casein kinase II phosphorylation site that resides within a conserved region in the La proteins from several species. RNA synthesis from isolated transcription complexes is inhibited by casein kinase II-mediated phosphorylation of La serine 366 and is reversible by dephosphorylation. This work demonstrates a novel mechanism of transcriptional control at the level of recycling of stable transcription complexes.
Assuntos
Autoantígenos/genética , Autoantígenos/metabolismo , RNA Polimerase III/genética , RNA Polimerase III/metabolismo , Ribonucleoproteínas/genética , Ribonucleoproteínas/metabolismo , Autoantígenos/isolamento & purificação , Western Blotting , Caseína Quinase II , Fracionamento Celular , Proteínas de Ligação a DNA/isolamento & purificação , Proteínas de Ligação a DNA/metabolismo , Células HeLa/enzimologia , Humanos , Focalização Isoelétrica , Espectrometria de Massas , Dados de Sequência Molecular , Mapeamento de Peptídeos , Fosforilação , Proteínas Serina-Treonina Quinases/isolamento & purificação , Proteínas Serina-Treonina Quinases/metabolismo , Ribonucleoproteínas/isolamento & purificação , Homologia de Sequência de Aminoácidos , Serina/metabolismo , Fatores de Transcrição/genética , Fatores de Transcrição/isolamento & purificação , Fatores de Transcrição/metabolismo , Transcrição Gênica/fisiologia , Antígeno SS-BRESUMO
Conversion of a nascent precursor tRNA to a mature functional species is a multipartite process that involves the sequential actions of several processing and modifying enzymes. La is the first protein to interact with pre-tRNAs in eukaryotes. An opal suppressor tRNA served as a functional probe to examine the activities of yeast and human (h)La proteins in this process in fission yeast. An RNA recognition motif and Walker motif in the metazoan-specific C-terminal domain (CTD) of hLa maintain pre-tRNA in an unprocessed state by blocking the 5'-processing site, impeding an early step in the pathway. Faithful phosphorylation of hLa on serine 366 reverses this block and promotes tRNA maturation. The results suggest that regulation of tRNA maturation at the level of RNase P cleavage may occur via phosphorylation of serine 366 of hLa.