Former laboratory dogs' psychological and behavioural characteristics.

It is becoming increasingly common for laboratories to allow the rehoming of dogs when they are no longer needed. There is no research, however, comparing the characteristics of dogs from laboratories to dogs acquired from more traditional sources for pets. This study used the Canine Behavioral Assessment and Research Questionnaire (C-BARQ) to compare emotional and behavioural characteristics of 100 former laboratory Beagles who were rehomed to a convenience sample of 244 pet Beagles with no known history of laboratory use. Our results revealed that the former laboratory Beagles exhibited increased fearfulness, attention and attachment behaviours, and more abnormal behaviours than the convenience sample, yet were significantly less aggressive, and likely to chase other animals or escape. Importantly, we also found no significant differences in a majority of the everyday behaviours assessed. Our findings demonstrate dogs rehomed from laboratories form strong bonds with their caregivers, and make equally good companions as their non-laboratory counterparts. Our findings may be helpful in designing appropriate rehabilitation programmes, educating laboratory workers and potential adopters, and support the ongoing efforts to offer these dogs for adoption.

Psychological Distress in Chimpanzees Rescued From Laboratories.

The United States is one of the last countries allowing invasive research on chimpanzees. Biomedical research on chimpanzees commonly involves maternal deprivation, social isolation, intensive confinement, and repetitive invasive procedures. These physically harmful and psychologically traumatic experiences cause many chimpanzees to develop symptoms of psychopathology that persist even after relocation from laboratories to sanctuaries. Through semistructured interviews with chimpanzee caregivers, direct behavioral observations, and consultation of laboratory records, we were interested in qualitatively analyzing symptoms of psychological distress in a sample of 253 chimpanzees rescued from biomedical research now residing at an accredited chimpanzee sanctuary. We present the results of this analysis and include an illustrative case study of one rescued chimpanzee who engages in self-injurious behaviors and meets modified Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition, criteria for posttraumatic stress disorder. We discuss our results in light of recent policy changes regarding the use of chimpanzees in biomedical research in the United States and their implications for those involved in the rescue and rehabilitation of chimpanzees from biomedical research.

The Benefits and Challenges of Conducting Primate Research in Different Settings.

Internationally, primate research takes place in laboratories, zoos, sanctuaries, and the wild. All of these settings present unique advantages and challenges in terms of methodology, translatability, animal welfare, and ethics. In this novel commentary, we explore the scientific and ethical benefits and drawbacks of conducting non-invasive psychological research with primates in each setting. We also suggest ways to overcome some of the barriers. We argue that while there may be greater experimental control in laboratory-based research, settings that more closely mirror primates' natural habitats are generally better suited to meet their specialized needs. More naturalistic research settings, including field studies, may also circumvent some ethical concerns associated with research in captivity, and yield more ecologically valid data.

Comparing the attractor strength of intra- and interpersonal interlimb coordination using cross-recurrence analysis.

Previous research has demonstrated that intra- and interpersonal rhythmic interlimb coordination are both constrained by the self-organizing entrainment process of coupled oscillators. Despite intra- and interpersonal coordination exhibiting the same stable macroscopic movement patterns the variability of the coordination is typically found to be much greater for inter- compared to intrapersonal coordination. Researchers have assumed that this is due to the interpersonal visual-motor coupling producing a weaker attractor dynamic than the intrapersonal neuromuscular coupling. To determine whether this assumption is true, two experiments were conducted in which pairs of participants coordinated hand-held pendulums swung about the wrist, either intra- and interpersonally. Using the cross-recurrence statistics of percent recurrence and maxline to independently index the level of noise and the attractor strength of the coordination, respectively, the results confirmed that the attractor strength was significantly weaker for inter- compared to intrapersonal coordination and that a similar magnitude of noise underlies both.

Period basin of entrainment for unintentional visual coordination.

Researchers have demonstrated that a person's rhythmic movements can become unintentionally entrained to another person's rhythmic movements or an environmental event. There are indications, however, that in both cases the likelihood of entrainment depends on the difference between the uncoupled periods of the two rhythms. The authors examined the range of period differences over which unintentional visual coordination might occur in 16 participants (Experiment 1) and 15 participants (Experiment 2). Cross-spectral coherence analysis and the distribution of continuous relative phase revealed that visual entrainment decreased as the difference between participants' preferred period and the experimenter-determined period of the environmental stimulus increased. The present findings extend the dynamical systems perspective on person-environment coupling and highlight the significance of period difference to the emergence of unintentional coordination.

Negative hysteresis in the behavioral dynamics of the affordance "graspable".

One commonly perceives whether a visible object will afford grasping with one hand or with both hands. In experiments in which differently sized objects of a fixed type are presented, the transition from using one of these manual modes to the other depends on the ratio of object size to hand span and on the presentation sequence, with size increasing versus decreasing. Conventional positive hysteresis (i.e., a larger transition ratio for the increasing sequence) can be accommodated by the order parameter dynamics that typify self-organizing systems (Lopresti-Goodman, Turvey, and Frank, Attention, Perception, & Psychophysics 73:1948-1965, 2011). Here we identified and addressed conditions of unconventional negative hysteresis (i.e., a larger transition ratio for the decreasing sequence). They suggest a second control parameter in the self-organization of affordance perception, one that is seemingly regulated by inhibitory dynamics occurring in the agent-task-environment system. Our experimental results and modeling extend the investigation of affordance perception within dynamical systems theory.

Stereotypical behaviors in chimpanzees rescued from the african bushmeat and pet trade.

Many orphaned chimpanzees whose mothers are illegally killed for their meat (bushmeat) in Africa are sold as pets or kept caged at hotels and businesses to attract tourists. As a result of being separated from their mothers and other chimpanzees at an early age, and spending years in impoverished captive conditions, some of these individuals engage in abnormal behaviors, including stereotypically scratching at their flesh and repetitively rocking back and forth. This paper presents case studies of Poco and Safari, two chimpanzees who were rescued by sanctuaries after living alone on display for humans at businesses for the first 7 to 8 years of their lives. Decades after their rescue, they still engage in stereotypical behaviors as a result of the psychological and physical trauma they endured early on. This paper combines data from in depth interviews with caregivers and direct observations of abnormal behaviors to assess psychological distress in captive-living chimpanzees. Our results highlight some lesser known harms of the bushmeat trade and the detrimental life-long consequences that keeping chimpanzees as "pets" can have on their mental health.

Behavioral dynamics of the affordance "graspable".

On a daily basis, one perceives whether an object affords grasping with one hand or with both hands. In experiments in which differently sized objects of a fixed type have been presented, the transition from using one manual mode to the other has depended on both the ratio of object size to hand span and the presentation sequence-that is, size increasing versus decreasing. The transitions and their observed hysteresis (i.e., a transition ratio larger for the increasing sequence) can be accommodated by the order parameter dynamics typifying self-organizing systems. Here, we show that hysteresis magnitude depends on (a) the interaction between the attractors (one hand vs. two hands) and (b) the strength of the two-hands attractor. Through modeling and experimental results, we extend the investigation of affordance perception within dynamical-systems theory.

Perceiving action-relevant properties of tools through dynamic touch: effects of mass distribution, exploration style, and intention.

At issue in the present series of experiments was the ability to prospectively perceive the action-relevant properties of hand-held tools by means of dynamic touch. In Experiment 1, participants judged object move-ability. In Experiment 2, participants judged how difficult an object would be to hold if held horizontally, and in Experiments 3 and 4, participants rated how fast objects could be rotated. In each experiment, the first and second moments of mass distribution of the objects were systematically varied. Manipulations of wielding speed and orientation during restricted exploration revealed perception to be constrained by (a) the moments of mass distribution of the hand-tool system, (b) the qualities of exploratory wielding movements, and (c) the intention to perceive each specific property. The results are considered in the context of the ecological theory of dynamic touch. Implications for accounts of the informational basis of dynamic touch and for the development of a theory of haptically perceiving the affordance properties of tools are discussed.

Task constraints on affordance boundaries.

The actualization of a simple affordance task-grasping and moving wooden planks of different sizes using either one or two hands-was assessed in the context of task-relevant (plank sequence, plank presentation speed) and task-irrelevant (cognitive load) manipulations. In Experiment 1, fast (3 s/plank) and self-paced ( approximately 5 s/plank) presentation speeds revealed hysteresis; the transition point for ascending series was greater than the transition point for descending series. Hysteresis was eliminated in the slowest presentation speed (10 s/plank). In Experiment 2, hysteresis was exaggerated by a cognitive load (counting backward by seven) for both fast and slow presentation speeds. These results suggest that behavioral responses to the attractor dynamics of perceived affordances are processes that require minimal cognitive resources.

Order parameter dynamics of body-scaled hysteresis and mode transitions in grasping behavior.

Several experimental studies have shown that human grasping behavior exhibits a transition from one-handed to two-handed grasping when to-be-grasped objects become larger and larger. The transition point depends on the relative size of objects measured in terms of human body-scales. Most strikingly, the transitions between the two different behavioral 'modes' of grasping exhibit hysteresis. That is, one-to-two hand transitions and two-to-one hand transitions occur at different relative object sizes when objects are scaled up or down in size. In our study we approach body-scaled hysteresis and mode transitions in grasping by exploiting the notion that human behavior in general results from self-organization and satisfies appropriately-defined order parameter equations. To this end, grasping transitions and grasping hysteresis are discussed from a theoretical perspective in analogy to cognitive processes defined by Haken's neural network model for pattern recognition. In doing so, issues such as the exclusivity of grasping modes, biomechanical constraints, mode-mode interactions, single subject behavior and population behavior are explored.

Acute alcohol intoxication enhances myocardial eIF4G phosphorylation despite reducing mTOR signaling.

Acute alcohol intoxication impairs myocardial protein synthesis in rats, secondary to a diminished mRNA translational efficiency. Decreased mRNA translational efficiency occurs through altered regulation of peptide chain initiation. The purpose of the present set of experiments was to determine whether acute alcohol intoxication alters the phosphorylation state of eukaryotic initiation factor (eIF) 4G, eIF4G.eIF4E complex formation, and the mammalian target of rapamycin (mTOR) signaling pathway in the heart. Acute alcohol intoxication was induced by injection of alcohol (75 mmol/kg body wt ip). Control animals received an equal volume of saline. Alcohol administration enhanced phosphorylation of eIF4G (Ser(1108)) approximately threefold. Alcohol administration lowered formation of the active eIF4G.eIF4E complex by >90%, whereas it increased the abundance of the inactive 4E-binding protein 1 (4E-BP1).eIF4E complex by approximately 160%. Phosphorylation of mTOR on Ser(2448) and Ser(2481) was decreased by 50%. Reduced mTOR phosphorylation did not result from decreased phosphorylation of PKB. Phosphorylation of 4E-BP1 and S6 kinase 1 (Thr(389)), downstream targets of mTOR, were also reduced after acute alcohol administration. These data suggest that acute alcohol-induced impairments in myocardial mRNA translation initiation result, in part, from marked decreases in eIF4G.eIF4E complex formation, which appear to be independent of changes in phosphorylation of eIF4G but dependent on mTOR.

Nutrient regulation of PKCepsilon is mediated by leucine, not insulin, in skeletal muscle.

Nutrients enhance signaling pathways involved in skeletal muscle growth through an increased rate of protein synthesis. These studies have led to an understanding of the potential role of the mammalian target of rapamycin (mTOR) in this process. However, activation of mTOR cannot account for all the stimulatory effects of nutrients. The purpose of these experiments was to examine the effect of nutrients on the cellular distribution and activation state of novel PKC isoforms (PKCepsilon and PKCdelta) in the gastrocnemius of rats by use of modification state-dependent phosphopeptide-specific antibodies. The phosphorylation of PKCepsilon on the catalytic domain autophosphorylation site (Ser(729)) was elevated during feeding and then returned to basal levels when the feeding period ended. Meal feeding augmented the phosphorylation of the downstream effectors of mTOR, namely S6K1 and 4E-BP1. In contrast, the phosphorylation of PKCdelta on either the catalytic domain autophosphorylation site (Ser(643)) or activation loop site (Thr(505)) was unaffected. Similar results were obtained when animals were given leucine either acutely via gavage or chronically by dietary supplementations. The effect of leucine was not mimicked by injecting animals with insulin but could be induced by gavage with norleucine, a structural analog of leucine that does not increase plasma insulin concentration. Thus rises in insulin secondary to meal intake or leucine gavage are probably not responsible for increased phosphorylation of PKCepsilon in response to meal feeding. Elevating the leucine concentration stimulated the phosphorylation of PKCepsilon in gastrocnemius from perfused hindlimb and caused a shift in the distribution of PKCepsilon from the membrane fraction to the cytosolic fraction. The results indicate that leucine leads to an activation (autophosphorylation) and subcellular redistribution of PKCepsilon, but not PKCdelta, in gastrocnemius both in vivo and in vitro. Furthermore, activation of the mTOR signaling pathway above basal conditions does not appear to be necessary to induce phosphorylation or translocation of PKCepsilon, suggesting that multiple signaling pathways become activated with leucine.