RESUMO
Enteral administration (every day, 10 times) of zinc aspartate (33 mg/kg) or zinc sulfate (25 mg/kg) to young rats weighing 50 - 60 g induces an amino acid imbalance in the blood plasma, liver, and myocardium. Zinc sulfate causes much more pronounced changes in the amino acid balance in the liver and myocardium, as compared to that in rats treated with zinc aspartate. One possible mechanism of the above changes can be the effect of zinc salts on nutrient absorption processes and metabolic features of zinc cations in the small intestine.
Assuntos
Aminoácidos/metabolismo , Ácido Aspártico/farmacologia , Intestino Delgado , Fígado , Miocárdio , Sulfato de Zinco/farmacologia , Animais , Intestino Delgado/metabolismo , Intestino Delgado/patologia , Fígado/metabolismo , Fígado/patologia , Miocárdio/metabolismo , Miocárdio/patologia , RatosRESUMO
Dose-dependent effects of monoclonal antibodies to tumor necrosis factor alpha (TNFa) in the form of infliximab preparation have been studied in Wistar rats upon with alcohol intoxication for 10 weeks (Lieber- De Carli liquid diet). It is established that the intraperitoneal administration of infliximab within the last 10 days of alcoholization in doses of 1 mg/kg and 10 mg/kg leads to dose-dependent changes in individual indices of the free amino acids pool and the amino acid balance in blood lymphocytes. Infliximab administered on the background of alcohol intoxication increases the pool of free amino acids and activates their metabolism in rat blood lymphocytes, which is probably due to inactivation of TNFalpha and adaptive changes in the amino acid transport system.
Assuntos
Intoxicação Alcoólica/sangue , Aminoácidos/metabolismo , Anti-Inflamatórios não Esteroides/farmacologia , Anticorpos Monoclonais/farmacologia , Linfócitos/química , Linfócitos/metabolismo , Aminoácidos/análise , Animais , Transporte Biológico/efeitos dos fármacos , Cromatografia Líquida de Alta Pressão , Doença Crônica , Relação Dose-Resposta a Droga , Etanol/farmacologia , Feminino , Infliximab , Injeções Intraperitoneais , Linfócitos/efeitos dos fármacos , Ratos , Ratos Wistar , Fator de Necrose Tumoral alfa/antagonistas & inibidoresRESUMO
The effect of infliximab (1 and 10 mg/kg, i.p., during 10 days) on alcoholic steatohepatitis has been studied in rats fed with ethanol-containing liquid diet over 10 weeks. Both doses of infliximab showed immunomodulatory and anti-inflammatory effects, whereas only the maximum drug dose significantly decreased lipid accumulation in the liver. It can be concluded that infliximab (10 mg/kg) acts as an effective hepatoprotector, anti-inflammatory agent, and immunomodulator in rats with experimental alcoholic steatohepatitis.
Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Anticorpos Monoclonais/farmacologia , Fígado Gorduroso Alcoólico/tratamento farmacológico , Fatores Imunológicos/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Animais , Depressores do Sistema Nervoso Central/efeitos adversos , Depressores do Sistema Nervoso Central/farmacologia , Modelos Animais de Doenças , Etanol/efeitos adversos , Etanol/farmacologia , Fígado Gorduroso Alcoólico/sangue , Fígado Gorduroso Alcoólico/etiologia , Feminino , Infliximab , Ratos , Ratos WistarRESUMO
Single intragastric injection of tritarg leads to different multidirectional changes in the concentration of free proteinogenic amino acids in the blood serum and lymphocytes isolated from the blood and liver. Changes in the amino acid stock of blood and liver lymphocytes were observed 3 and 24 hours after the drug injection. The changes in concentrations of individual free amino acids are more pronounced in liver lymphocytes than in blood lymphocytes. There is a decrease in the content of proteinogenic amino acids in blood serum, which may reflect the supply of these compounds to cells and is indicative of the stimulation of polypeptide and protein synthesis.
Assuntos
Aminoácidos/sangue , Suplementos Nutricionais , Linfócitos/efeitos dos fármacos , Biossíntese de Proteínas/efeitos dos fármacos , Administração Oral , Animais , Arginina/administração & dosagem , Asparagina/administração & dosagem , Fígado/citologia , Fígado/metabolismo , Linfócitos/citologia , Linfócitos/metabolismo , Masculino , Ratos , Taurina/administração & dosagem , Fatores de Tempo , Triptofano/administração & dosagem , Zinco/administração & dosagemRESUMO
A mixture of leucine and zinc sulfate (4 : 1; 100 mg/kg body weight) produces a hepatoprotective effect, preventing the ultrastructural injury of hepatic tissue and the disturbance of free amino acid metabolism caused by a toxic dose of paracetamol.
Assuntos
Acetaminofen/antagonistas & inibidores , Analgésicos não Narcóticos/antagonistas & inibidores , Citoproteção , Leucina/farmacologia , Fígado/efeitos dos fármacos , Sulfato de Zinco/farmacologia , Acetaminofen/toxicidade , Aminoácidos/sangue , Analgésicos não Narcóticos/toxicidade , Animais , Combinação de Medicamentos , Feminino , Fígado/metabolismo , Fígado/ultraestrutura , Ratos , Ratos WistarRESUMO
The intraperitoneal injection of taurine--zinc sulfate composition (400 mg/kg body weight) led to a considerable increase in the plasma taurine levels, activated the hepatic uptake of free amino acids, and modulated the metabolism of sulfur-containing amino acids.
Assuntos
Aminoácidos/metabolismo , Fígado/metabolismo , Taurina/farmacologia , Sulfato de Zinco/farmacologia , Aminoácidos/sangue , Aminoácidos Essenciais/sangue , Aminoácidos Essenciais/metabolismo , Aminoácidos Sulfúricos/sangue , Aminoácidos Sulfúricos/metabolismo , Animais , Combinação de Medicamentos , Masculino , Ratos , Ratos WistarRESUMO
Administration of infliximab (a monoclonal antibody to tumor necrosis factor-a 1-10 mg/kg for 10 days) to Wistar rats consuming ethanol for 10 weeks significantly changed levels of free amino acids and their nitrogenous metabolites in liver lymphocytes.