Effect of electrokinetic process on in situ stabilization and detoxification of arsenic-contaminated soil with high content of calcium.

Owing to the high risk of human exposure to arsenic-contaminated soil, reducing its toxicity is essential. This study used the electrokinetic (EK) process with iron-rich electrodes to synchronously achieve the accumulate, stabilize and detoxify soil arsenic. Changes in arsenic valence, leaching toxicity, and microbial communities related to toxicity were comprehensively considered. The results demonstrated that arsenic was mainly transported toward the anode and accumulated by electromigration owing to the negatively charged arsenic anions under EK conditions. The cathode approaching effectively promote arsenic movement to the anode; the largest As(T) transportation rate of 30.61% was achieved near the cathode (S4). The transportation ratio of As(III) was 1.84 times more than that of As(V). The As(III) content and leaching toxicity of soil arsenic in all treatments decreased after applying the EK process. In particular, the anode approaching effectively elevated the average ratios of soil As(III) oxidation and stabilization to 37.88% and 61.73%, respectively. Correspondingly, the total phospholipid fatty acid content increased substantially after EK treatment and showed a pollution stress elimination effect. The electrokinetic effect can essentially cause highly active and easily migrated arsenic to accumulate near the anode and middle sections. The electric field mediated iron mineralization and stabilized arsenic by oxidizing As(III) and reacting with newly formed iron-rich phases (S). Meanwhile, the electric field regulated the form of soil calcium from CaCO3 to CaSO4 and caused calcium-bound arsenic to change to a more stable form. According to these results, in situ stabilization and detoxification of arsenic-contaminated soil can be realized by the EK process, avoiding stabilizer addition and excavation.

Response characteristics and functional predictions of soil microorganisms to heavy metals, antibiotics, and their resistance genes originating from different animal farms amended with Herbaspirillum huttiense.

Current understanding is limited regarding technologies that use biochar and microorganisms to simultaneously treat soils contaminated with both veterinary antibiotics (VAs) and heavy metals (HMs) from different animal farms. The contributions of the keystone taxa and their similarities from different animal farms under VA and HM stresses before and after soil remediation should be further investigated as well. An innovative treatment of Herbaspirillum huttiense (HHS1) inoculated waste fungus chaff-based (WFCB) biochar was designed for immobilization of copper (Cu) and zinc (Zn), and the removal of oxytetracycline (OTC), enrofloxacin (ENR), and a subsequent reduction in their resistance genes in soils from pig, cow, and chicken farms. Roles of indigenous microorganisms which can treat soils contaminated with VAs and HMs were summarized. Results showed that available Cu and Zn were reduced by 19.5% and 28.1%, respectively, while 49.8% of OTC and 85.1% of ENR were removed by WFCB-HHS1. The decrease in ENR improved overall microbial community diversity, and the increases in genera HHS1, Pedobacter, Flavobacterium and Aequorivita, along with the decreases of genera Bacillus, Methylobacter, and Fermentimonas were indirectly favorable to treat HMs and VAs in soils from different animal farms. Bacterial communities in different animal farm soils were predominantly influenced by stochastic processes. The regulations of functional genes associated with metabolism and environmental information processing, which contribute to HM and VA defense, were altered when using WFCB-HHS1. Furthermore, the spread of their antibiotic resistance genes was restricted.

Highly efficient screening and optimal combination of functional isolates for bioremediation of hydrocarbon-polluted soil.

The key to enhancing the efficacy of bioremediation of hydrocarbon-contaminated soil is the precise and highly efficient screening of functional isolates. Low screening effectiveness, narrow screening range and an unstable structure of the constructed microflora during bioremediation are the shortcomings of the traditional shaking culture (TSC) method. To improve the secondary screening of isolates and microflora implemented for alkane degradation, this work evaluated the characterization relationship between bacterial function and enzyme activity and devised an enzyme activity assay (EAA) method. The results indicated a substantial positive correlation (r = 0.97) between 24 candidate isolates and their whole enzymes, proving that whole enzyme activity properly reflects the metabolic functions of microorganisms. The functional analysis of the isolates demonstrated that the EAA method in conjunction with microbial abundance and metabolite determination could broaden the screening range of functional isolates, including aliphatic acid-metabolizing isolates (isolates H4 and H7) and aliphatic acid-sensitive isolates (isolate H2) with n-hexadecane degradation ability. The EAA method also guided the construction of functional microflora and optimized the mode of application using combinations of alkane-degrading bacteria and aliphatic acid-degrading bacteria successively (e.g., F1+H7+H7). The combinations maintained a high abundance of functional isolates and stable α diversity and community composition throughout the experiment, which contributed to more advanced alkane degradation and mineralization ability (p < 0.01). Assuming a workload of 100 tests, the screening efficiency of the EAA method is more than 16 times that of the TSC method, and the greater the quantity of isolates, the higher the screening efficiency, enabling high-throughput screening. In conclusion, the EAA method has a broad-spectrum, accurate and highly efficient screening ability for functional isolates and microflora, which can provide intensive technical support for the development of bioremediation materials and the application of bioremediation technology.

Assessment of the human health risk of polycyclic aromatic hydrocarbons in soils from areas of crude oil exploitation.

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous carcinogenic pollutants. Areas where crude oil has been exploited are at risk due to PAHs from both natural and anthropogenic sources. The Shengli Oilfield in China was used to assess the health risk posed by PAHs in areas with different population densities. A risk assessment showed that in the areas with low, median, and high population densities, the probabilities of the total carcinogenic risk (TCR) exceeding 10-6 for adults were 9.9%, 9.3%, and 13.4%, respectively, whereas these were 7.8%, 7.1%, and 10.1%, respectively, for children. Crude oil, traffic, and residential emissions were the major sources of PAHs based on a factor analysis with a nonnegative constraint analysis. Crude oil sources accounted for 96.1% of the TCR in the low population area, whereas traffic accounted for 94.4% of the TCR in the high population area. Based on the national action plan, guidelines, and new standard for soil pollution control promulgated by the Chinese government, we assumed a reduced rate of soil PAHs from different sources, and the carcinogenic risk from PAHs in the area of crude oil exploitation could be forecast. The average probabilities of the TCR exceeding 10-6 for adults and children could be reduced by 45.8% and 49.4%, respectively, in 2040 relative to current values under a pollution control scenario. These findings highlight that the risks associated with soil contamination could be effectively controlled by implementing control policies.

Influence of electrical fields enhanced phytoremediation of multi-metal contaminated soil on soil parameters and plants uptake in different soil sections.

The influence of electrical fields on phytoremediation of multi-metal (Cd, Cu, and Zn) naturally contaminated soils has been investigated based on different soil sections. After ryegrass and hybrid penisetum were sowed for 30 d, electrical fields were applied during 30 days with the switching polarity every 30 min and continuing for 16 h d-1. After electrokinetic (EK) assisted phytoremediation process, soil electrical conductivity (EC) in anode section and available soil potassium (K) in cathode section were obviously elevated. Plants biomass in middle and cathode sections were increased in both plants, especially in middle section the overall biomass of hybrid penisetum increased by 68.8%. The influence of electrical field on the contents of heavy metals in plants was different depending on the species of plants, kind of heavy metals and soil section. For Cd, Cu, and Zn co-contaminated soils, shoot metals accumulation in middle section in both plants were improved at least about 20% (with the exception of Zn in ryegrass). Electrical fields had the most significant effect on copper absorption by ryegrass and shoot Cu accumulation were elevated 32.5% in all the section. The soil EC maybe an important factor that affected electrical fields enhanced plants growth, plant metals concentrations and remediation efficiency.

Enhancement of electrokinetic-bioremediation by ryegrass: Sustainability of electrokinetic effect and improvement of n-hexadecane degradation.

Phytoremediation-assisted electrokinetic-bioremediation is a novel technology for soil remediation. We aimed to study the effects of a plant (ryegrass) on electrokinetic-bioremediation in n-hexadecane-contaminated soil. After treatment for 40 days, the n-hexadecane degradation ratio of electrokinetic-bioremediation-ryegrass (EK-Bio-RG) was 4.86% higher than that of electrokinetic-bioremediation (EK-Bio) (p < 0.05), with a maximum constant degradation rate (107.23 ± 4.62 mg kg-1· d-1). Owing to the improved electrical conductivity, 73.28% of the initial current was maintained on the 40th day in EK-Bio-RG, which was 1.62 times that in EK-Bio. Furthermore, ryegrass reduced the soil zeta potential, which indicated the alleviation of the soil electric double layer compression and prevention of the aggregation of small soil colloids into larger ones. The fine colloidal structure was conducive to mass transfer in electrokinetic-bioremediation. An analysis of the microbial community showed that the degradation of n-hexadecane was mainly attributable to gram-positive bacteria, and a new microbial community was gradually constructed in the rhizosphere, which still metabolized n-hexadecane. The results indicated that the sustainability of the electrokinetic effect was improved combined with ryegrass, and the harmonious micro-environment in the rhizosphere was constructed which furtherly optimized the EK-Bio technology to remediate organics-polluted soil.

Distribution of ion contents and microorganisms during the electro-bioremediation of petroleum-contaminated saline soil.

This study investigated the distribution of ion contents and microorganisms during the electro-bioremediation (EK-Bio) of petroleum-contaminated saline soil. The results showed that soil ions tend to accumulate around the electrodes, and the concentration was correlated with the distance from the electrodes. The average soil ion content was 7.92 g/kg around the electrodes (site A) and 0.55 g/kg at the furthest distance from the electrodes (site B) after 112 days of treatment, while the initial average content was 3.92 g/kg. Smooth linear (R2 = 0.98) loss of soil ions was observed at site C, which was closer to the electrodes than site B, and had a final average soil ion content of 1.96 g/kg. The dehydrogenase activity was much higher in EK-Bio test soil than in the Bio test soil after 28 days of treatment, and followed the order: site C > site B > site A. However, the soil dehydrogenase activity dropped continuously when the soil ion reached very high and low concentrations at sites A and B. The soil microbial community varied in sample sites that had different ion contents, and the soil microbial diversity followed the order: site C > site B > site A. The applied electric field clearly enhanced the biodegradation efficiency for soil petroleum contaminants. However, the biodegradation promotion effects were weakening in soils where the ion contents were extremely high and low (sites A and B). These results can provide useful information for EK-Bioremediation of organic-contaminated saline soil.

Isolation and characterization of heavy polycyclic aromatic hydrocarbon-degrading bacteria adapted to electrokinetic conditions.

Polycyclic aromatic hydrocarbon (PAH)-degrading bacteria capable of growing under electrokinetic conditions were isolated using an adjusted acclimation and enrichment procedure based on soil contaminated with heavy PAHs in the presence of an electric field. Their ability to degrade heavy PAHs under an electric field was individually investigated in artificially contaminated soils. The results showed that strains PB4 (Pseudomonas fluorescens) and FB6 (Kocuria sp.) were the most efficient heavy PAH degraders under electrokinetic conditions. They were re-inoculated into a polluted soil from an industrial site with a PAH concentration of 184.95 mg kg(-1). Compared to the experiments without an electric field, the degradation capability of Pseudomonas fluorescens and Kocuria sp. was enhanced in the industrially polluted soil under electrokinetic conditions. The degradation extents of total PAHs were increased by 15.4 and 14.0% in the electrokinetic PB4 and FB6 experiments (PB4 + EK and FB6 + EK) relative to the PB4 and FB6 experiments without electrokinetic conditions (PB4 and FB6), respectively. These results indicated that P. fluorescens and Kocuria sp. could efficiently degrade heavy PAHs under electrokinetic conditions and have the potential to be used for the electro-bioremediation of PAH-contaminated soil, especially if the soil is contaminated with heavy PAHs.

An assessment of the effectiveness and impact of electrokinetic remediation for pyrene-contaminated soil.

The effectiveness of electrokinetic remediation for pyrene-contaminated soil was investigated by an anode-cathode separated system using a salt bridge. The applied constant voltage was 24 V and the electrode gap was 24 cm. Two types of soil (sandy soil and loam soil) were selected because of their different conductive capabilities. The initial concentrations of pyrene in these soil samples were 261.3mg/kg sandy soil and 259.8 mg/kg loam soil. After treatment of the sandy soil and loam soil for seven days, 56.8% and 20.1% of the pyrene had been removed respectively. Under the same power supply voltage, the removal of the pollutant from the sandy soil was greater than that from the loam soil, due to the higher current and lower pH. Further analysis revealed that the effectiveness of electrokinetic remediation was affected by the energy expenditure, and was associated with changes in soil properties.

Construction of an effective method combining in situ capping with electric field-enhanced biodegradation for treating PAH-contaminated soil at abandoned coking sites.

The simultaneous application of in situ capping and electro-enhanced biodegradation may be a suitable method for ensuring the feasibility and safety of reusing abandoned coking sites. However, the capping layer type and applied electric field pattern may affect the efficiency of sequestering and removing pollutants. This study investigated changes in electric current, soil moisture content and pH, polycyclic aromatic hydrocarbon (PAH) concentration, bacterial number, and microbial community structure and metabolic function during soil remediation at abandoned coking plant sites under different applied electric field patterns and barrier types. The results indicated that polarity-reversal electric field was more conducive to maintaining electric current, soil properties, resulting in higher microbial number, community diversity, and functional gene abundance. At 21d, the mean PAH concentrations in contaminated soil, the capping layer's clean soil and barrier were 78.79, 7.56, and 1.57 mg kg-1 lower than those with a unidirectional electric field, respectively. The mean degradation rate of PAHs in the bio-barrier was 10.12 % higher than that in the C-Fe barrier. In the experiment combining a polarity-reversal electric field and a bio-barrier, the mean PAH concentrations in contaminated soil and the capping layer were 706.68 and 27.15 mg kg-1 lower than those in other experiments, respectively, and no PAHs were detected in the clean soil, demonstrating that the combination of the polarity-reversal electric field and the bio-barrier was effective in treating soil at abandoned coking plant sites. The established method of combining in situ capping with electro-enhanced biodegradation will provide technical support for the treatment and reuse of heavily PAH-contaminated soil at abandoned coking plant sites.

Multipath diffusion process and spatial accumulation simulation of Cd in lead-zinc mining areas.

This study combined process simulation and actual measurement to construct a multipath diffusion and spatial accumulation model of Cd in a typical lead-zinc mining area through accuracy and root mean square error(RMSE) analysis. The results indicated that (1) the diffusion of Cd was in a quadratic inverse proportional relationship with the distance from the pollution source within watershed. The average annual atmospheric Cd sedimentation in study area was 0.71 * 10-6 g and the contribution of runoff diffusion to Cd exceeded 80%. (2) With the increase in the concentration range of Cd content (k) carried by unit runoff sediment, the model accuracy and RMSE showed decreasing trends. However, when the lower and upper limits of k were 10% and 90%, the model accuracy reached 75%. (3) Two sub-watersheds with same dominant wind direction but different runoff directions were selected to verify the model accuracy, indicating that the model construction method can precisely simulate the spatial accumulation of Cd in similar mining areas. The results provide a scientific basis for the prevention of heavy metal diffusion in lead-zinc mines. Future research should focus on the migration pathways of heavy metals through vertical infiltration caused by rainfall to further optimise the model structure and accuracy.

Effects of electrokinetic operation mode on removal of polycyclic aromatic hydrocarbons (PAHs), and the indigenous fungal community in PAH-contaminated soil.

Electrokinetic remediation is an emerging physical remediation technology for the removal of heavy metals and organic chemicals from contaminated soil. We set up a soil chamber (24 × 12 × 8 cm) with two stainless steel electrodes (12 × 0.5 cm), and a constant voltage gradient of 1.0 v cm(-1) or 2.0 v cm(-1) was applied to study the effects of unidirectional and altered directional electric field operation modes on the moisture content and pH, the removal rate of PAHs, and the abundance and diversity of indigenous fungi in a PAH-contaminated soil at the Benxi Iron and Steel Group Corporation (N41°17'24.4″, E123°43'05.8″), Liaoning Province, Northeast China. Electrokinetic remediation increased the PAH removal rate, but had less effect on soil moisture content and pH, in comparison with the control. In the 1 v cm(-1) altered directional operation, in particular, the PAH removal rate by the end of the experiment (on day 23) had increased from 5.2% of the control to 13.84% and 13.69% at distances of 4 and 20 cm from the anode, respectively, and to 18.97% in the middle region of the soil chamber. On day 23, the indigenous fungal 18S rRNA gene copy numbers and community diversity were significantly higher in a voltage gradient of 1 v cm(-1) than in a voltage gradient 2 v cm(-1). An altered directional operation was more conducive to the fungal community's uniform distribution than was a unidirectional operation of the electric field. We found the major PAH-degrading fungi Fusarium oxysporum and Rhizophlyctis rosea to be present under EK remediation. We suggest that a 1 v cm(-1) altered directional operation could be an appropriate electrokinetic operation mode for PAH removal, and the maintenance of abundance and diversity of the indigenous fungal community.

Effects of soil organic carbon metabolism on electro-bioremediation of petroleum-contaminated soil.

Soil organic carbon (SOC) potentially interacts with microbial metabolism and may affect the degradation of petroleum-derived carbon (PDC) in the electro-bioremediation of petroleum-contaminated soil. This study evaluated the interactions among organic carbon, soil properties, and microbial communities to explore the role of SOC during the electro-bioremediation process. The results showed that petroleum degradation exerted superposition and synergistic electrokinetic and bioremediation effects, as exemplified by the EB and EB-PR tests, owing to the maintenance and enhancement of SOC utilization (P/S value), respectively. The highest P/S value (2.0-2.4) was found in the electrochemical oxidation zone due to low SOC consumption. In the biological oxidation zones, electric stimulation enhanced the degradation of PDC and SOC, with higher average P/S values than those of the Bio test. Soil pH, Eh, inorganic ions, and bioavailable petroleum fractions were the main factors reshaping the microbial communities. SOC metabolism effectively buffered the stress of environmental factors and pollutants while maintaining functional bacterial abundance, microbial alpha diversity, and community similarity, thus saving the weakened PDC biodegradation efficiency in the EB and EB-PR tests. The study of the effect of SOC metabolism on petroleum biodegradation contributes to the development of sustainable low-carbon electro-bioremediation technology.

Source apportionment of heavy metals in the soil at the regional scale based on soil-forming processes.

Source apportionment is crucial to the prevention and control of heavy metals in the soil. The major methods focus on the identification of soil heavy metals from different pollution sources. However, they are unsuited to the source apportionment at a regional scale due to ignoring the spatial heterogeneity of heavy metal content caused by soil formation. Thus, we built a source apportionment model by introducing the weathering and leaching coefficients as the key parameters of soil-forming processes. In this study, we selected Liaohe Plain in China as the study area, which was the starting point of China's industrial development, with dense industrial areas and high levels of heavy-metal emission. Heavy metals concentrations in surface and deep soil of reference and grid points were collected as model data. The results showed that the average contribution rates of soil-forming process to Cd, Hg, As, and Pb were 82.7%, 85.2%, 88.6%, and 91.7%, respectively, and those of anthropogenic activities were 17.3%, 14.8%, 11.4%, and 8.3%, respectively. Spatial distribution of contribution rates showed the superposition of soil environmental background and pollution sources. This study provides a feasible method to quantify heavy metals contents from natural and anthropogenic sources at a regional scale.

Treatment of PAHs contaminated soil in abandoned industrial site using combined method of improved in situ capping and electrokinetic enhanced-bioremediation.

In situ capping and bioremediation are common technologies for treating contaminated soil at industrial sites. However, these two technologies have some shortcomings for treating soil heavily contaminated with organic matter, such as the limited adsorption in capping layer and the low biodegradation efficiency. This study proposed the method of an improved in situ capping combined with electrokinetic enhanced-bioremediation, and investigated its feasibility for treating heavily polycyclic aromatic hydrocarbons (PAHs) contaminated soil at an abandoned industrial site. By analyzing the changes in soil properties, PAHs concentration, and microbial community in experiments with voltages of 0, 0.8, 1.2, and 1.6 V cm-1, it was found that improved in situ capping could effectively sequester PAHs migration by adsorption and biodegradation, and electric field could enhance PAHs removal from contaminated soil and bio-barrier. In the experiments with electric field, soil environment under the voltage of 1.2 V cm-1 was more favorable for the growth and metabolism of microorganisms, and the residual PAHs concentrations (19.47 ± 0.76 mg kg-1 and 619.38 ± 20.05 mg kg-1) in the bio-barrier and contaminated soil of experiment with 1.2 V cm-1 were the lowest, which indicated that optimization of the electric field conditions could lead to better effects.

Effect of electric fields strength on soil factors and microorganisms during electro-bioremediation of benzo[a]pyrene-contaminated soil.

Electro-bioremediation is a promising technology for remediating soils contaminated with polycyclic aromatic hydrocarbons (PAHs). However, the resulting electrokinetic effects and electrochemical reactions may inevitably cause changes in soil factors and microorganism, thereby reducing the remediation efficiency. To avoid negative effect of electric field on soil and microbes and maximize microbial degradability, it is necessary to select a suitable electric field. In this study, artificial benzo [a]pyrene (BaP)-contaminated soil was selected as the object of remediation. Changes in soil factors and microorganisms were investigated under the voltage of 1.0, 2.0, and 2.5 V cm-1 using chemical analysis, real-time PCR, and high-throughput sequencing. The results revealed noticeable changes in soil factors (pH, moisture, electrical conductivity [EC], and BaP concentration) and microbes (PAHs ring-hydroxylating dioxygenase [PAHs-RHDα] gene and bacterial community) after the application of electric field. The degree of change was related to the electric field strength, with a suitable strength being more conducive to BaP removal. At 70 d, the highest mean extent of BaP removal and PAHs-RHDα gene copies were observed in EK2.0 + BIO, reaching 3.37 and 109.62 times those in BIO, respectively, indicating that the voltage of 2.0 V cm-1 was the most suitable for soil microbial growth and metabolism. Changes in soil factors caused by electric fields can affect microbial activity and community composition. Redundancy analysis revealed that soil pH and moisture had the most significant effects on microbial community composition (P < 0.05). The purpose of this study was to determine the appropriate electric field that could be used for electro-bioremediation of PAH-contaminated soil by evaluating the effects of electric fields on soil factors and microbial communities. This study also provides a reference for efficiency enhancement and successful application of electro-bioremediation of soil contaminated with PAHs.

Effects of thermal desorption on ecotoxicological characteristics of heavy petroleum-contaminated soil.

This study comprehensively evaluates the ecotoxicity of high-concentration heavy petroleum (HCHP)-contaminated soil before and after thermal desorption (TD) remediation at different temperatures and times. The results showed that the detoxification of contaminated soil was effectively achieved by extending the remediation duration at 400-600 °C. After treatment at 400 °C for 60 min, the toxicological indicators including bioluminescence EC50 (acute toxicity), seed germination ratio (Gr) and plant biomass of Brassica juncea (subacute toxicity), and diversity of the microbial community (chronic toxicity) reached a maximum. The value of the SOS-Induction Factor (SOSIF), characterizing genotoxicity was below 1.5, indicating that it was non-toxic. Pearson's correlation analysis illustrated that the water-soluble fraction (WSF), ALK1-3 and ARO1-3 of petroleum hydrocarbons were the primary sources of ecotoxicity. Notably, although the total ratio of petroleum removed from the soil reached 87.26 ± 4.38 %-98.69 ± 1.61 % under high-temperature thermal desorption (HTTD, 500-600 °C), the ecotoxicity was not lower than that at 400 °C. The pyrolysis products of petroleum macromolecules and extreme changes in soil properties were the leading causes of soil ecotoxicity following HTTD. The inconsistency between the removal of petroleum pollutants and ecological health risks reveals the significance of soil ecotoxicological assessments for identifying TD remediation endpoints and process optimization.

New insight into desorption behavior and mechanism of oil from aged oil-contaminated soil in microemulsion.

The intractable nature of oil-contaminated soil (OS) constitutes the chief limiting factor for its remediation. Herein, the aging effect (i.e., oil-soil interactions and pore-scale effect) was investigated by analyzing the properties of aged OS and further demonstrated by investigating the desorption behavior of the oil from the OS. XPS was performed to detect the chemical environment of N, O, and Al, indicating the coordination adsorption of carbonyl groups (oil) on the soil surface. Alterations in the functional groups of the OS were detected using FT-IR, indicating that the oil-soil interactions were enhanced via wind-thermal aging. SEM and BET were used to analyze the structural morphology and pore-scale of the OS. The analysis revealed that aging promoted the development of the pore-scale effect in the OS. Moreover, the desorption behavior of oil molecules from the aged OS was investigated via desorption thermodynamics and kinetics. The desorption mechanism of the OS was elucidated via intraparticle diffusion kinetics. The desorption process of oil molecules underwent three stages: film diffusion, intraparticle diffusion, and surface desorption. Owing to the aging effect, the latter two stages constituted the major steps for controlling oil desorption. This mechanism provided theoretical guidance to apply microemulsion elution for remedying industrial OS.

Effects of mineral species and petroleum components on thermal desorption behavior of petroleum-contaminated soil.

Thermal desorption (TD) behavior of high-concentration petroleum-contaminated soil (PCS) is affected by soil composition, especially inorganic minerals. In this study, the TD behavior of petroleum-contaminated quartz (original mineral) and kaoline (clay mineral) were compared with those of pure petroleum (Petro-free); their "saturate, aromatic, resin, and asphaltenes" (SARA) fractions were investigated using thermogravimetry and differential thermogravimetry (TG-DTG). The modelling of the petroleum removal kinetics was also analyzed to provide insights into the mechanism. The results revealed that the limiting factor controlling the desorption of petroleum from quartz (Petro-Qtz) and kaoline (Petro-Kln) is the minerals, which increased the effective TD temperature by 200 °C and decreased TD efficiency by 2%. Compared to Petro-Qtz, Petro-Kln showed a lower desorption efficiency of 5% and the process was accomplished at a higher temperature of 100 °C. The investigation on SARA fractions indicated that polar fractions (i.e., aromatics, resins, and asphaltenes) were strongly captured by the minerals. The increment of the TD temperature of petroleum (resins-160 °C > aromatics-20 °C > saturates-5 °C) increased with the polarity of petroleum components. These results could be validated by thermogravimetry-gas chromatography/mass spectroscopy (TG-GC/MS) through the delayed desorption of naphthalene and acenaphthene. Furthermore, the increment of the TD temperature of SARA fractions on kaoline was higher than those on quartz. This makes sense because the kaoline decreased the diffusion of hydrocarbons due to its porosity features and higher specific surface area (kaoline 5.3300 m2 g-1, quartz 0.1153 m2 g-1). In addition, the analysis of the desorption kinetic models showed that the observed hysteresis was related to the diffusion barrier caused by chemisorption (n＜1). In consequence, the Petro-Kln showed a lower desorption efficiency, a slower desorption, and as a result, a higher energy consumption (0.476 kW h) for thermal remediation than Petro-Qtz (0.238 kW h).

Improvement of the electrokinetic fluxes by tall fescue: Alleviation of ion attenuation and maintainability of soil colloidal properties.

In this study, we aimed to address the attenuation of electrokinetic fluxes that occur during plant (tall fescue)-based electrokinetic remediation of oil-contaminated soil. Following 60 days of treatment, the concentration of water-soluble cations and anions in the electrokinetics-assisted phytoremediation treatment (EK-P) were 20.03 mg/kg and 15.7 mg/kg higher than that in the electrokinetic (EK) treatment, respectively. At the electrode, plants were able to alleviate the ion aggregation effect caused by the electrokinetics, reduce the conversion of soluble ions to insoluble ones, and reduce the decay of water-soluble ions. In addition, the zeta potential of EK-P was 5.05 mV lower than that of EK. Plants maintained the stability of the soil colloid and reduced the movement of the peak of colloidal particle size from small to large particles, thereby reducing the amount of colloidal deposition. Finally, the EK-P current was 22.49% higher than that in EK while the electrokinetic effect was maintained. Meanwhile, electrokinetics increased plant biomass by 20.21%. Electrokinetics was found to create a synergy with the plants, an effect that eventually enhanced the rate of oil degradation.