Biochar-based functional materials for the abatement of emerging pollutants from aquatic matrices.

Biochar has emerged as a versatile and efficient multi-functional material, serving as both an adsorbent and catalyst in removing emerging pollutants (EPs) from aquatic matrices. However, pristine biochar's catalytic and adsorption capabilities are hindered by its poor surface functionality and small pore size. Addressing these limitations involves the development of functionalized biochar, a strategic approach aimed at enhancing its physicochemical properties and improving adsorption and catalytic efficiencies. Despite a growing interest in this field, there is a notable gap in existing literature, with no review explicitly concentrating on the efficacy of biochar-based functional materials (BCFMs) for removing EPs in aquatic environments. This comprehensive review aims to fill this void by delving into the engineering considerations essential for designing BCFMs with enhanced physiochemical properties. The focus extends to understanding the treatment efficiency of EPs through mechanisms such as adsorption or catalytic degradation. The review systematically outlines the underlying mechanisms involved in the adsorption and catalytic degradation of EPs by BCFMs. By shedding light on the prospects of BCFMs as a promising multi-functional material, the review underscores the imperative for sustained research efforts. It emphasizes the need for continued exploration into the practical implications of BCFMs, especially under environmentally relevant pollutant concentrations. This holistic approach seeks to contribute to advancing knowledge and applying biochar-based solutions in addressing the challenges posed by emerging pollutants in aquatic ecosystems.

Comparative study of the removal of sulfate by UASB in light and dark environment.

At present, the application of sewage treatment technologies is restricted by high sulfate concentrations. In the present work, the sulfate removal was biologically treated using an upflow anaerobic sludge blanket (UASB) in the absence/presence of light. First, the start-up of UASB for the sulfate removal was studied in terms of COD degradation, sulfate removal, and effluent pH. Second, the impacts of different operation parameters (i.e., COD/SO42- ratio, temperature and illumination time) on the UASB performance were explored. Third, the properties of sludge derived from the UASB at different time were analyzed. Results show that after 28 days of start-up, the COD removal efficiencies in both the photoreactor and non-photoreactor could reach a range of 85-90% while such reactors could achieve > 90% of sulfate being removed. Besides, higher illumination time could facilitate the removal of pollutants in the photoreactor. To sum up, the present study can provide technical support for the clean removal of sulfate from wastewater using photoreactors.

Transcriptomic and Hormonal Changes in Wheat Roots Enhance Growth under Moderate Soil Drying.

Understanding the mechanisms that regulate plant root growth under soil drying is an important challenge in root biology. We observed that moderate soil drying promotes wheat root growth. To understand whether metabolic and hormonic changes are involved in this regulation, we performed transcriptome sequencing on wheat roots under well-watered and moderate soil drying conditions. The genes upregulated in wheat roots under soil drying were mainly involved in starch and sucrose metabolism and benzoxazinoid biosynthesis. Various plant hormone-related genes were differentially expressed during soil drying. Quantification of the plant hormones under these conditions showed that the concentrations of abscisic acid (ABA), cis-zeatin (CZ), and indole-3-acetic acid (IAA) significantly increased during soil drying, whereas the concentrations of salicylic (SA), jasmonic (JA), and glycosylated salicylic (SAG) acids significantly decreased. Correlation analysis of total root length and phytohormones indicated that CZ, ABA, and IAA are positively associated with wheat root length. These results suggest that changes in metabolic pathways and plant hormones caused by moderate soil drying help wheat roots grow into deeper soil layers.

Ways to mitigate greenhouse gas production from rice cultivation.

Rice cultivation boasts a rich historical legacy, serving as the primary sustenance for over 50% of the global population. However, the cultivation process gives rise to the emission of methane (CH4) and nitrous oxide (N2O), two potent greenhouse gases. Notably, the global warming potential (GWP) of CH4 and N2O surpasses CO2 by 27-30 times and 273 times over 100 years, respectively. Addressing this environmental challenge necessitates exploring technical approaches and management strategies to curb gas emissions while sustaining rice yields. Several critical factors have been identified and analyzed for their potential to mitigate greenhouse gas production during rice cultivation. These include water management, fertilizer management, biochar application, cultivar selection, straw management, modified planting methods, and integration of new energy machinery. A comprehensive understanding and implementation of these methods can contribute significantly to achieving a dual objective: reducing emissions and maintaining optimal rice yields. Looking ahead, a synergistic integration of these diverse methods and management approaches holds promise for more effective results. Furthermore, the intricate water networks associated with rice cultivation should be carefully considered in the overall strategy. By adopting a holistic approach that addresses both emission reduction and sustainable water usage, the future of rice cultivation can be shaped to align with environmental stewardship and food security.

Magnetic biochar serves as adsorbents and catalyst supports for the removal of antibiotics from wastewater: A review.

Numerous antibiotics are being released into the natural environment through wastewater. As antibiotic usage increases annually, its detrimental impact on the environment is escalating. Addressing environmental sustainability and human health requires significant attention towards antibiotic removal. In recent years, magnetic biochar (MBC) has gained widespread application in water treatment due to its exceptional adsorption and catalytic degradation capabilities. Antibiotics such as sulfamethoxazole (SMX), tetracycline (TC), ciprofloxacin (CIP), and others commonly exhibit an adsorption capacity by MBC ranging from 5 mg/g to 900 mg/g. Moreover, MBC typically removes over 90% of these antibiotics within 60 min. The effectiveness of antibiotic removal is significantly influenced by various preparation and modification methods. Furthermore, the incorporation of magnetism enables the material to be recycled and reused multiple times, thereby reducing consumption costs. This article discusses recent studies on antibiotic removal using MBC. It has been observed that variations in the selection of raw material and preparation procedures significantly affect antibiotic removal, while the mechanisms involved in antibiotic removal remain ambiguous. Additionally, it has been noted that the removal process may lead to secondary pollution and high preparation costs. Therefore, this review comprehensively outlines the utilization of MBC in the removal of antibiotics from wastewater, including aspects such as modification, preparation, removal mechanism, and factors influencing removal, and providing recommendations for antibiotic development. The aim is to offer researchers a clear understanding to advance the field of MBC materials.

Integrated assessment of available water volume for sustainable sponge city construction - A case study in Xi'an, China.

To address the lack of theoretical guidance for sponge city construction (SCC) in China, this study introduces a method to evaluate the available water volume (AWV) in urban watersheds. This evaluation is based on the water balance relationship, water volume, and ecological water demand (EWD). The Xi'an urban area was selected as a case study due to its water shortage and flooding issues. Results show monthly surface and subsurface AWV ranging between 53.06 and 53.98 million m3 and between 8,701.89 and 8,898.14 million m3, respectively. By maximizing the potential for surface AWV, an annual water supply of 527.75 million m3 could be provided, surpassing the annual artificial water consumption of 394.20 million m3, effectively addressing water scarcity. During the rainy season, implementing measures such as employing permeable paving materials, establishing wetlands and rainwater gardens, and constructing lakes and reservoirs can mitigate flooding caused by rainfall exceeding 32.8 mm. While the subsurface space in Xi'an holds significant potential for subsurface AWV utilization, revitalizing the ecological environment of subsurface water is crucial. Overall, the AWV theoretical framework offers a comprehensive solution to water shortage and flooding issues in the Xi'an urban area, serving as a vital theory for SCC.

An in-situ biochar-enhanced anaerobic membrane bioreactor for swine wastewater treatment under various organic loading rates.

A biochar-assisted anaerobic membrane bioreactor (BC-AnMBR) was conducted to evaluate the performance in treating swine wastewater with different organic loading rates (OLR) ranging from 0.38 to 1.13 kg-COD/(m3.d). Results indicated that adding spent coffee grounds biochar (SCG-BC) improved the organic removal efficiency compared to the conventional AnMBR, with an overall COD removal rate of > 95.01%. Meanwhile, methane production of up to 0.22 LCH4/gCOD with an improvement of 45.45% was achieved under a high OLR of 1.13 kg-COD/(m3.d). Furthermore, the transmembrane pressure (TMP) in the BC-AnMBR system was stable at 4.5 kPa, and no irreversible membrane fouling occurred within 125 days. Microbial community analysis revealed that the addition of SCG-BC increased the relative abundance of autotrophic methanogenic archaea, particularly Methanosarcina (from 0.11% to 11.16%) and Methanothrix (from 16.34% to 24.05%). More importantly, Desulfobacterota and Firmicutes phylum with direct interspecific electron transfer (DIET) capabilities were also enriched with autotrophic methanogens. Analysis of the electron transfer pathway showed that the concentration of c-type cytochromes increased by 38.60% in the presence of SCG-BC, and thus facilitated the establishment of DIET and maintained high activity of the electron transfer system even at high OLR. In short, the BC-AnMBR system performs well under various OLR conditions and is stable in the recovery energy system for swine wastewater.

In-situ production of lactate driving the biotransformation of waste activated sludge to medium-chain fatty acid.

Medium-chain fatty acids (MCFAs) have drawn great attention due to their high energy density and superior hydrophobicity. Waste activated sludge (WAS) has been documented as a renewable feedstock for MCFAs production via anaerobic fermentation. However, MCFAs production from WAS depends on exogenous addition of electron donor (ED, e.g., lactate) for chain elongation (CE) bioprocess, which results in increased economic cost and limited practical application. In this study, a novel biotechnology was proposed to produce MCFAs from WAS with in-situ self-formed lactate by inoculating Yoghurt starter powder containing with Lactobacillales cultures. The batch experimental results revealed that the lactate was in-situ generated from WAS and the maximum production of MCFAs increased from 1.17 to 3.99 g COD/L with the increased addition of Lactobacillales cultures from 6✕107 to 2.3✕108 CFU/mL WAS. In continuous long-term test over 97 days, average MCFA production reached up to 3.94 g COD/L with a caproate yield of 82.74% at sludge retention time (SRT) 12 days, and the average MCFA production increased to 5.87 g COD/L with 69.28% caproate and 25.18% caprylate at SRT 15 days. A comprehensive analysis of the metagenome and metatranscriptome demonstrated that the genus of Lactobacillus and Streptococcus were capable of producing lactate from WAS and upgrading to MCFAs. Moreover, another genus, i.e., Candidatus Promineofilum, was firstly revealed that it might be responsible for lactate and MCFAs production. Further investigation of related microbial pathways and enzyme expression suggested that D-lactate dehydrogenase and pyruvate ferredoxin oxidoreductase contributed to lactate and acetyl-CoA production, which were the crucial steps for MCFAs generation and were most actively expressed. This study provides a conceptual framework of MCFAs from WAS with endogenous ED, potentially enhancing the energy recovery from WAS treatment.

Application of Chlorella vulgaris for nutrient removal from synthetic wastewater and MBR-treated bio-park secondary effluent: growth kinetics, effects of carbon and phosphate concentrations.

Application of Chlorella vulgaris for polishing secondary effluent of a wastewater treatment (containing C, N and P) was investigated. As a first step, batch experiments were conducted in Bold's Basal Media (BBM) to quantify the effects of orthophosphates (0.1-107 mg/L), organic carbon (0-500 mg/L as acetate) and N/P ratio on the growth of Chlorella vulgaris. The results revealed that the orthophosphate concentration was found to control the removal rates of nitrates and phosphates; however, both were effectively removed (> 90%) when the initial orthophosphate concentration was 4-12 mg/L. The maximum nitrate and orthophosphate removals were observed at an N:P ratio of ~ 11. However, the specific growth rate (µ) was significantly increased (from 0.226 to 0.336 g/g/day) when the initial orthophosphate concentration was 0.1-4.3 mg/L. On the other hand, the presence of acetate had significantly improved the specific growth and specific nitrate removal rates of Chlorella vulgaris. The specific growth rate increased from 0.34 g/g/day in a purely autotrophic culture to 0.70 g/g/day in the presence of acetate. Subsequently, the Chlorella vulgaris (grown in BBM) was acclimated and grown in the membrane bioreactor (MBR)-treated real-time secondary effluent. Under the optimised conditions, 92% nitrate and 98% phosphate removals (with a growth rate of 0.192 g/g/day) were observed in the bio-park MBR effluent. Overall, the results indicate that coupling Chlorella vulgaris as a polishing treatment in existing wastewater treatment units could be beneficial for highest level of water reuse and energy recovery goals.

Effective fluorine removal using mixed matrix membrane based on polysulfone: adsorption performance and diffusion behavior.

Fluorine is one of the essential trace elements for human life activities, but excessive intake of fluoride poses a great risk to people's health. In this paper, a series of mixed matrix membrane (MMM)-based polysulfone for removing fluoride were prepared by phase inversion, and their properties, adsorption capacity, adsorption isotherms, adsorption kinetics of fluoride ions, and mechanism were all investigated. The results confirmed that the MMM contained a large number of hydroxyl and aluminum functional groups due to resin being added. The MMM exhibited the best fluorine ion adsorption capacity of 2.502 mg/g at a pH of 6 with the initial concentration of 6 mg/L. As well, adsorption kinetics of fluorine ion on MMM followed the pseudo-second-order model, while the adsorption behavior of fluorine ion on MMM was well simulated by the Langmuir isotherm model. The adsorption capacity of MMM remained stable after six cycles and the regeneration efficiency was still above 80%, resulting in a long-term stability adequate for fluorine ion removal. Complexation and ion exchange played a key role in the fluorine ion adsorption of MMM. These results indicated the MMM as novel type of absorbent had an excellent capacity for removing fluoride.

Sustainable enzymatic technologies in waste animal fat and protein management.

Waste animal fats and proteins (WAFP) are rich in various animal by-products from food industries. On one hand, increasing production of huge amounts of WAFP brings a great challenge to their appropriate disposal, and raises severe risks to environment and life health. On the other hand, the high fat and protein contents in these animal wastes are valuable resources which can be reutilized in an eco-friendly and renewable way. Sustainable enzymatic technologies are promising methods for WAFP management. This review discussed the application of various enzymes in the conversion of WSFP to value-added biodiesel and bioactivate hydrolysates. New biotechnologies to discover novel enzymes with robust properties were proposed as well. This paper also presented the bio-utilization strategy of animal fat and protein wastes as alternative nutrient media for microorganism growth activities to yield important industrial enzymes cost-effectively.

Intensive removal of PAHs in constructed wetland filled with copper biochar.

In this study, biochar-loading copper ions (Cu-BC), a novel composite for removing phenanthrene very efficiently from water, was prepared using the impregnation method. The performance of constructed wetlands (CWs) with these modified and original biochar as substrates was analyzed. CW with Cu-BC removed a large amount of phenanthrene (94.09 ± 3.02%). According to the surface characteristics analysis, Cu-BC can promote the removal of pollutants via complex absorption, hydrophobic adsorption, increasing the Lewis Pair and electrostatic attraction. Furthermore the higher nitrate removal rate in the treated system (91.11 ± 1.17%) was observed to have higher levels of bacterial metabolic diversity and denitrifier types. The phenanthrene accumulated in plants with this treatment system was enhanced by the role of copper in photosynthesis. It is able to boost the plant extraction of organic matter.

A new model framework for sponge city implementation: Emerging challenges and future developments.

Sponge City concept is emerging as a new kind of integrated urban water systems, which aims to address urban water problems. However, its implementation has encountered a variety of challenges. The lack of an integrated comprehensive model to assist Sponge City planning, implementation and life cycle assessment is one of the most challenging factors. This review briefly analyses the opportunity of existing urban water management models and discusses the limitation of recent studies in the application of current integrated models for Sponge City implementation. Furthermore, it proposes a new Sponge City model framework by integrating four main sub-models including MIKE-URBAN, LCA, W045-BEST, and MCA in which environmental, social, and economic aspects of Sponge City infrastructure options are simulated. The new structure of Sponge City model that includes the sub-model layer, input layer, module layer, output layer, and programing language layer is also illustrated. Therefore, the proposed model could be applied to optimize different Sponge City practices by not only assessing the drainage capacity of stormwater infrastructure but also pays attention to multi-criteria analysis of urban water system (including the possibility of assessing Sponge City ecosystem services for urban areas and watershed areas) as well. Balancing between simplification and innovation of integrated models, increasing the efficiency of spatial data sharing systems, defining the acceptability of model complexity level and improving the corporation of multiple stakeholders emphasizing on possible future directions of a proper Sponge City design and construction model.

Performance of mediator-less double chamber microbial fuel cell-based biosensor for measuring biological chemical oxygen.

Recently, the microbial fuel cell-based biosensor has been considered as an attractive technology for measuring wastewater quality such as biochemical oxygen demand (BOD). In this study, a mediator-less double compartment MFC based biosensor utilizing carbon felt as an anode electrode and inoculated with mixed culture was developed to improve the real application of a rapid BOD detection. This study aims to: (i) establish the effect of the operating conditions (i.e., pH, external resistance, fuel feeding rate) on MFC performance; (ii) investigate the correlation between biochemical oxygen demand (BOD) and signal output, and (iii) evaluate the operational stability of the biosensor. The presented result reveals that the maximum current and power production was obtained while 100 mM NaCl and 50 mM Phosphate buffer saline was used as a catholyte solution, neutral pH condition of media and fuel feeding rate at 0.3 mL min-1. Notably, a wider range of BOD concentration up to 300 mg L -1 can be obtained with the voltage output (R2 > 0.9901). Stable and steady power was produced by running MFC in 30 days when cells operated at 1000 Ω external resistance. Our research has some competition with the previous double chamber MFC in the upper limit of BOD detection. This results might help to increase the real application of MFC based BOD biosensor in real-time measurement.

Substrate Diffusion within Biofilms Significantly Influencing the Electron Competition during Denitrification.

A common and long-existing operational issue of wastewater denitrification is the unexpected accumulation of nitrite (NO2-) that could suppress the activity of various microorganisms involved in biological wastewater treatment process and nitrous oxide (N2O) that could emit as a potent greenhouse gas. Recently, it has been confirmed that the accumulation of these denitrification intermediates in biological wastewater treatment process is greatly influenced by the electron competition between the four denitrification steps. However, little is known about this in biofilm systems. In this work, we applied a mathematical model that links carbon oxidation and nitrogen reduction processes through a pool of electron carriers, to assess electron competition in denitrifying biofilms. Simulations were performed comprehensively at seven combinations of electron acceptor addition scheme (i.e., simultaneous addition of one, two or three among nitrate (NO3-), NO2-, and N2O) to compare the effect of electron competition on NO3-, NO2- and N2O reduction. Overall, the effects of substrate loading, biofilm thickness and effective diffusion coefficients on electron competition are not always intuitive. Model simulations show that electron competition was intensified due to the substrate load limitation (from 120 to 20 mg COD/L) and increasing biofilm thicknesses (from 0.1 to 1.6 mm) in most cases, where electrons were prioritized to nitrite reductase because of the insufficient electron donor availability in the biofilm. In contrast, increasing effective diffusion coefficients did not pose a significant effect on electron competition and only increased electrons distributed to nitrite reductase when both NO2- and N2O are added.

Heterotrophic denitrifiers growing on soluble microbial products contribute to nitrous oxide production in anammox biofilm: Model evaluation.

In this work, a model framework was constructed to assess and predict nitrous oxide (N2O) production, substrate and microbe interactions in an anammox biofilm bioreactor. The anammox kinetics were extended by including kinetics of autotrophic soluble microbial products (SMP) formation, which consisted of utilization-associated products (UAP) and biomass-associated products (BAP). Heterotrophic bacteria growing on UAP, BAP and decay released substance (SS) were modelled to perform four-step sequential reductions from nitrate to dinitrogen gas. N2O was modelled as an intermidiate of heterotrophic denitrification via three pathways with UAP, BAP and SS as the electron donors. The developed model framework was evaluated using long-term operational data from an anammox biofilm reactor and satisfactorily reproduced effluent nitrogen and SMP as well as N2O emission factors under different operational conditions. The modeling results revealed that N2O was mainly produced with UAP as the electron donor while BAP and SS play minor roles. Heterotrophic denitrifiers growing on UAP would significantly contribute to N2O emission from anammox biofilm reactor even though heterotrophs only account for a relatively small fraction of active biomass in the anammox biofilm. Comprehensive simulations were conducted to investigate the effects of N loading rate and biofilm thickness, which indicated that maintaining a low N loading rate and a thick biofilm thickness were essential for high total nitrogen removal efficiency and low N2O emission.

Modeling electron competition among nitrogen oxides reduction and N2 O accumulation in hydrogenotrophic denitrification.

Hydrogenotrophic denitrification is a novel and sustainable process for nitrogen removal, which utilizes hydrogen as electron donor, and carbon dioxide as carbon source. Recent studies have shown that nitrous oxide (N2 O), a highly undesirable intermediate and potent greenhouse gas, can accumulate during this process. In this work, a new mathematical model is developed to describe nitrogen oxides dynamics, especially N2 O, during hydrogenotrophic denitrification for the first time. The model describes electron competition among the four steps of hydrogenotrophic denitrification through decoupling hydrogen oxidation and nitrogen reduction processes using electron carriers, in contrast to the existing models that couple these two processes and also do not consider N2 O accumulation. The developed model satisfactorily describes experimental data on nitrogen oxides dynamics obtained from two independent hydrogenotrophic denitrifying cultures under various hydrogen and nitrogen oxides supplying conditions, suggesting the validity and applicability of the model. The results indicated that N2 O accumulation would not be intensified under hydrogen limiting conditions, due to the higher electron competition capacity of N2 O reduction in comparison to nitrate and nitrite reduction during hydrogenotrophic denitrification. The model is expected to enhance our understanding of the process during hydrogenotrophic denitrification and the ability to predict N2 O accumulation.

Fluoride removal from water using a magnesia-pullulan composite in a continuous fixed-bed column.

A magnesia-pullulan composite (MgOP) was previously shown to effectively remove fluoride from water. In the present study, a continuous fixed-bed column was used to examine the application of the composite at an industrial scale. The influencing parameters included bed mass (4.0, 6.0 and 8.0 g), influent flow rate (8, 16 and 32 mL/min), inlet fluoride concentration (5, 10 and 20 mg/L), reaction temperature (20, 30 and 40 °C), influent pH (4, 7 and 10) and other existing anions (HCO3-, SO42-, Cl- and NO3-), through which the breakthrough curves could be depicted for the experimental data analysis. The results indicated that MgOP is promising for fluoride removal with a defluoridation capacity of 16.6 mg/g at the bed mass of 6.0 g, influent flow rate of 16 mL/min and inlet fluoride concentration of 10 mg/L. The dynamics of the fluoride adsorption process were modeled using the Thomas and Yan models, in which the Yan model presented better predictions for the breakthrough curves than the Thomas model. Moreover, the concentration of magnesium in the effluent was monitored to determine Mg stability in the MgOP composite. Results indicated the effluent concentration of Mg2+ ions could be kept at a safe level. Calcination of fluoride-loaded MgOP effectively regenerated the material.

Performance, microbial community and fluorescent characteristic of microbial products in a solid-phase denitrification biofilm reactor for WWTP effluent treatment.

Microbial products, i.e. extracellular polymeric substance (EPS) and soluble microbial product (SMP), have a significant correlation with microbial activity of biologically based systems. In present study, the spectral characteristics of two kinds of microbial products were comprehensively evaluated in a solid-phase denitrification biofilm reactor for WWTP effluent treatment by using poly (butylene succinate) (PBS) as carbon source. After the achievement of PBS-biofilm, nitrate and total nitrogen removal efficiencies were high of 97.39 ±â€¯1.24% and 96.38 ±â€¯1.1%, respectively. The contents of protein and polysaccharide were changed different degrees in both LB-EPS and TB-EPS. Excitation-emission matrix (EEM) implied that protein-like substances played a significant role in the formation of PBS-biofilm. High-throughput sequencing result implied that the proportion of denitrifying bacteria, including Simplicispira, Dechloromonas, Diaphorobacter, Desulfovibrio, increased to 9.2%, 7.4%, 4.8% and 3.6% in PBS-biofilm system, respectively. According to EEM-PARAFAC, two components were identified from SMP samples, including protein-like substances for component 1 and humic-like and fulvic acid-like substances for component 2, respectively. Moreover, the fluorescent scores of two components expressed significant different trends to reaction time. Gas chromatography-mass spectrometer (GC-MS) implied that some new organic matters were produced in the effluent of SP-DBR due to biopolymer degradation and denitrification processes. The results could provide a new insight about the formation and stability of solid-phase denitrification PBS-biofilm via the point of microbial products.

Industrial metal pollution in water and probabilistic assessment of human health risk.

Concentration of eight heavy metals in surface and groundwater around Dhaka Export Processing Zone (DEPZ) industrial area were investigated, and the health risk posed to local children and adult residents via ingestion and dermal contact was evaluated using deterministic and probabilistic approaches. Metal concentrations (except Cu, Mn, Ni, and Zn) in Bangshi River water were above the drinking water quality guidelines, while in groundwater were less than the recommended limits. Concentration of metals in surface water decreased as a function of distance. Estimations of non-carcinogenic health risk for surface water revealed that mean hazard index (HI) values of As, Cr, Cu, and Pb for combined pathways (i.e., ingestion and dermal contact) were >1.0 for both age groups. The estimated risk mainly came from the ingestion pathway. However, the HI values for all the examined metals in groundwater were <1.0, indicating no possible human health hazard. Deterministically estimated total cancer risk (TCR) via Bangshi River water exceeded the acceptable limit of 1 × 10-4 for adult and children. Although, probabilistically estimated 95th percentile values of TCR exceeded the benchmark, mean TCR values were less than 1 × 10-4. Simulated results showed that 20.13% and 5.43% values of TCR for surface water were >1 × 10-4 for adult and children, respectively. Deterministic and probabilistic estimations of cancer risk through exposure to groundwater were well below the safety limit. Overall, the population exposed to Bangshi River water remained at carcinogenic and non-carcinogenic health threat and the risk was higher for adults. Sensitivity analysis identified exposure duration (ED) and ingestion rate (IR) of water as the most relevant variables affecting the probabilistic risk estimation model outcome.