Phytoremediation through microstructural and functional alterations in alkali weed (Cressa cretica L.) in the hyperarid saline desert.

Salt excretory halophytes are the major sources of phytoremediation of salt-affected soils. Cressa cretica is a widely distributed halophyte in hypersaline lands in the Cholistan Desert. Therefore, identification of key physio-anatomical traits related to phytoremediation in differently adapted C. cretica populations was focused on. Four naturally adapted ecotypes of non-succulent halophyte Cressa cretica L. form hyper-arid and saline desert Cholistan. The selected ecotypes were: Derawar Fort (DWF, ECe 20.8 dS m-1) from least saline site, Traway Wala Toba (TWT, ECe 33.2 dS m-1) and Bailah Wala Dahar (BWD, ECe 45.4 dS m-1) ecotypes were from moderately saline sites, and Pati Sir (PAS, ECe 52.4 dS m-1) was collected from the highly saline site. The natural population of this species was collected and carefully brought to the laboratory for different structural and functional traits. As a result of high salinity, Na+, Cl-, K+, and Ca2+ content significantly increased at root and shoot level. At root level, some distinctive modifications such as increased sclerification in vascular bundles, enlarged vascular bundles, metaxylem vessels, phloem region, and storage parenchyma (cortex) are pivotal for water storage under extreme arid and osmotic condition. At the stem level, enhanced sclerification in outer cortex and vascular bundles, stem cellular area, cortical proportion, metaxylem and phloem area, and at the leaf level, very prominent structural adaptations were thicker and smaller leaves with increased density of salt glands and trichomes at surface, few and large stomata, reduced cortical and mesophyll parenchyma, and narrow xylem vessels and phloem area represent their non-succulent nature. The ecotype collected from hypersaline environments was better adapted regarding growth traits, ion uptake and excretion, succulence, and phytoremediation traits. More importantly, structural and functional traits such as root length and biomass, accumulation of toxic ions along with K+ in root and shoot, accumulation of Ca2+ in shoot and Mg2+ in root, excretion of toxic ions were the highest in this ecotype. In conclusion, all these alterations strongly favor water conservation, which certainly contributes to ecotypes survival under salt-induced physiological drought.

Naturally adapted salt tolerant plants provide exceptional material for exploring adaptive mechanisms they use to confront high salt concentrations. Cressa cretica is a hypersaline hyperarid desert colonizer, which was previously underexplored. In the present study, we focused on the new insight on relationship among anatomical modifications, salt accumulation and excretion and phytoremediation potential of this rare species.

Ion homeostasis in differently adapted populations of Suaeda vera Forssk. ex J.F. Gmel. for phytoremediation of hypersaline soils.

Salt-accumulator species are of great interest for the phytoremediation of salt-affected soils to reclaim soil salinization, a major constraints causing germination retardation and growth restriction of plants as well as habitat degradation. Higher biomass production at ECe 23-36 dS m-1 indicated that this species grows better in high to moderate salinity that was linked to osmotic adjustment through higher ion accumulation (Na+, Cl‒, and Ca2+) and organic osmolytes (free amino acids and proline). Plants from highly and moderately saline habitats exhibited broader metaxylem vessels, which was associated with eased conduction of solutes leading to better growth. Leaf anatomical characteristics generally increased with increasing salinity except at the highest ECe 55 dS m-1. The increased leaf lamina thickness contributed to succulence because of increased storage parenchymatous spongy tissues (that can store high amounts of water), water contents and it is a reflection of maintaining ion homeostasis and colonizing hyper-saline soil. Reduced stomatal density and area under high salinity are critical to cope with environmental hazards. Under high salinity, compartmentalization of excessive Na+ and Cl- ions and accumulation of compatible osmolytes are directly related to high degree of salinity tolerance, and hence are useful for phyto-amelioration of salinity-impacted lands.

Salinity tolerance mechanism in Suaeda vera have been explored extensively, but these mechanisms have not been addressed in the context of soil-plant interaction and functioning connection between structural and functional features with respect to phytoremediation. This work aims to provide mechanistic insight into how different morphological and physio-anatomical mechanisms in differently adapted population of Suaeda vera operate in coordinated manner to maintain ionic homeostasis under high salinity that are useful for phytoremediation.

Structural determinants of phytoremediation capacity in saltmarsh halophyte Diplachne fusca (L.) P. Beauv. ex Roem. & Schult. subsp. fusca.

Micro and macro-morphological features contribute to plants' tolerance to a variety of environmental pollutants. The contribution of such structural modifications in the phytoremediation potential of Diplachne fusca populations collected from five saline habitats were explored when treated with 100 to 400 mM NaCl for 75 days along with control. Structural modifications in the populations from the highest salinity included development of aerenchyma in stem instead of chlorenchyma, absence of excretory hairs in stem, and exceptionally large trichomes on the leaf surface to help excretion of excess salt. Large parenchyma cells provided more space for water and solute storage, while broad metaxylem vessels were linked to better conduction water and nutrients, which ultimately excreted via glandular hairs, microhairs, and vesicular hairs. Broad metaxylem vessels and exceptionally long hairs observed in the populations collected from 52 dS m-1. In conclusion, large stem aerenchyma, exceptionally large trichomes on the leaf surface, and tightly packed outer cortical region in roots with intensive sclerification just inside the epidermis accompanied with salt excretion via glandular hairs, microhairs, and vesicular hairs were the main anatomical modifications involved in the phytoremediation potential of D. fusca in hyper-saline environments.

Morpho-anatomical characteristics of the differently adapted populations of Diplachne fusca has never been reported. In particular, structural variation in their mechanism of adaptation for salinity tolerance was investigated for the first time in current study.

Cytotoxicity against A549 Human Lung Cancer Cell Line via the Mitochondrial Membrane Potential and Nuclear Condensation Effects of Nepeta paulsenii Briq., a Perennial Herb.

The genus Nepeta belongs to the largest Lamiaceae family, with 300 species, which are distributed throughout the various regions of Africa, Asia, India, and America. Along with other plant families distinguished by their medicinal and therapeutic values, the Nepeta genus of Lameaceae remains relatively valuable. Hence, the phytochemicals of N. paulsenii Briq. were extracted using different plant parts, i.e., leaves, stem, roots, flowers, and the whole plant by using various solvents (ethanol, water, and ethyl acetate), obtaining 15 fractions. Each extract of dried plant material was analyzed by FT-IR and GC-MS to identify the chemical constituents. The cytotoxicity of each fraction was analyzed by MTT assay and mitochondrial membrane potential and nuclear condensation assays against lung cancer cells. Among the ethyl acetate and ethanolic extracts, the flowers showed the best results, with IC50 values of 51.57 µg/mL and 50.58 µg/mL, respectively. In contrast, among the water extracts of the various plant segments, the stem showed the best results, with an IC50 value of 123.80 µg/mL. 5-flourouracil was used as the standard drug, providing an IC50 value of 83.62 µg/mL. The Hoechst 33342 stain results indicated apoptotic features, i.e., chromatin dissolution and broken down, fragmented, and crescent-shaped nuclei. The ethanolic extracts of the flowers showed more pronounced apoptotic effects on the cells. The mitochondrial membrane potential indicated that rhodamine 123 fluorescence signals suppressed mitochondrial potential due to the treatment with the extracts. Again, the apoptotic index of the ethanolic extract of the flowers remained the highest. Hence it can be concluded that the flower part of N. paulsenii Briq. was found to be the most active against the A459 human lung cancer cell line.

Aridity-driven changes in structural and physiological characteristics of Buffel grass (Cenchrus ciliaris L.) from different ecozones of Punjab Pakistan.

Cenchrus ciliaris L. is a perennial grass that can grow in a diverse range of habitats including challenging deserts. The purpose of the study was to investigate the impact of aridity on morpho-anatomical and physiological traits in C. ciliaris populations collected from arid and semi-arid areas of Punjab, Pakistan. The populations growing in extremely arid conditions displayed a range of structural and physiological adaptations. Under extremely dry conditions, root epidermal thickness (90.29 µm), cortical cell area (7677.78 µm2), and metaxylem cell area (11,884.79 µm2) increased while root pith cell area (2681.96 µm2) decreased in tolerant populations. The populations under extremely aridity maximized leaf lamina (184.21 µm) and midrib thickness (316.46 µm). Additionally, highly tolerant populations were characterized by the accumulation of organic osmolytes such as glycinebetaine (132.60 µmol g-1 FW) was increased in QN poulations, proline (118.01 µmol g-1 F.W) was maximum in DF populations, and total amino acids (69.90 mg g-1 FW) under extreme water deficit conditions. In arid conditions, abaxial stomatal density (2630.21 µm) and stomatal area (8 per mm2) were also reduced in DF populations to check water loss through transpiration. These findings suggest that various parameters are crucial for the survival of C. ciliaris in arid environments. The main strategies used by C. ciliaris was intensive sclerification, effective retention of ions, and osmotic adjustment through proline and glycinebetaine under arid conditions. Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-023-01351-3.

Foreign body aspiration in an adult: An endobronchial "Melon-oma".

Background: Foreign body (FB) aspirations in adults are relatively uncommon. The most commonly aspirated FBs in adults are organic, especially vegetable matter, peanuts, and fragments of bones. We report a rare case of a FB discovered in the left main bronchus of an adult male admitted to the intensive care unit. Case report: A 52-year-old male smoker with COPD presented to the emergency department with a two-day history of increasing dyspnea and cough. He was hypoxic and febrile with a temperature of 38°C. Auscultation revealed decreased breath sounds over the left lung and a few rhonchi on the right side. Chest x-ray showed left lung collapse. His condition rapidly worsened, and he was immediately intubated for acute respiratory failure. CT chest identified a large endobronchial mass obstructing the left main bronchus. Flexible bronchoscopy confirmed a soft and mobile brownish lesion in the left main bronchus. The histological appearance of the specimen retrieved was consistent with an organic foreign body. This was later identified as a melon chunk. It was removed successfully via flexible bronchoscopy by cutting it into smaller pieces to aid retrieval. Conclusion: FB aspiration can occur in all age groups but is less common in adults accounting for only 0.16%-0.33% of adult bronchoscopic procedures. Early detection of an aspirated FB is essential to avoid significant complications, morbidity, and mortality.

Pre-procedural SARS-CoV-2 PCR testing in the pulmonary function laboratory at a tertiary government hospital in Qatar: A clinical audit.

BACKGROUND: Prior to pulmonary function testing (PFT), local and international recommendations advise pre-procedural screening. Pulmonary function tests generate aerosol droplets containing millions of viruses, significantly increasing the risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission not only to the individuals in and around the PFT office, but also to subsequent patients who undergo the test later in the same room. METHODS: This clinical audit was carried out to establish the rate of positive pre-procedural SARS-CoV-2 PCR testing before a PFT. The data were obtained over a 6-week period from our ATS accredited pulmonary function laboratory at the Hamad General Hospital, Qatar (December 01, 2021, to January 10, 2022). The PFT laboratory was closed from January 10, 2022, till the date of this report (January 27, 2022) owing to an increase in COVID cases in the community in Qatar during the fourth wave. RESULTS: All the patients scheduled for PFT were asymptomatic of COVID-19. A total of 331 individuals were scheduled for PFT, and 221 PFTs were performed. There were 109 no-shows for both the PCR and the PFT. Between weeks 1 and 4, all the pre-procedural SARS-CoV-2 PCR tests were negative. The weekly average number of COVID-19 cases in Qatar increased from 157 per 100,000 population in week 1 to 2,918 in week 6.2 There was a similar trend in the pre-procedural SARS-CoV-2 PCR tests that increased and resulted in identifying 9 cases with positive SARS-CoV-2 PCR test over weeks 5 and 6 (Figure 1). CONCLUSION: As the number of documented positive SARS-CoV-2 PCR tests in the community grew, so did the pre-procedural COVID-19 PCR positivity and the number of no-shows. The large number of no-shows may indicate greater worry or concern about contracting COVID-19 when visiting the hospital amid peak community cases. Our findings further call into question the utility of routinely performing pre-procedural PCR screening in asymptomatic cases when the prevalence of COVID-19 is low in the local population. Perhaps, it is time to consider replacing this with on-the-spot quick antigen testing for more effective use of resources.

Functional traits for salinity tolerance in differently adapted populations of Fimbristylis complanata (Retz.).

Functional modifications in three populations of Fimbristylis complanata collected from differently salt effected habitats were evaluated. The populations were established in pots and treated with five NaCl levels (0, 100, 200, 300, and 400 mM). Population SH (collected from the highest salinities, ECe 37.94 dS m-1) exhibited better osmotic adjustment because of the higher accumulation of organic osmolytes under high salinities and was ranked as highly tolerant. Other features like an increased concentration of chlorophyll pigments ensured maintenance of photosynthetic capability, and accumulated higher K+ and Ca2+ contents that minimized the toxic effect of Na+ and maintained ion homeostasis. Salinity tolerance in the Lillah-Khewra foothills (LR) population (collected from moderately saline site, ECe 31.36 dS m-1) relied on the maintenance of shoot dry weight (SDW) and shoot and root length (RL) with a parallel accumulation of organic osmolytes and shoot Ca2+. This species is a stem succulent and can store excessive amount of salt in storage parenchyma, as indicated by the accumulation of high concentration of Na+ in shoot. The SH population, in particular, can be rated as the best for phytoremediation of salt-affected soils that accumulated more Na+ than other populations and concentration of osmolytes for turgor maintenance under high salinities. Novelty statement Fimbristylis is less explored, particularly no information available on salt tolerance of F. complanata exists in the literature.

Physiological, Biochemical and Defense System Responses of Roadside Vegetation to Auto-Exhaust Pollution.

This study determined the effects of traffic pollutants on plants (Nerium oleander and Ricinus communis) growing along Faisalabad to Okara (R-1) and Okara to Lahore (R-2) roads in Pakistan. The photosynthetic pigments, photosynthetic rate, transpiration rate and total soluble proteins of roadside vegetation were significantly lower than control plants (50 m away from road). The average decrease in photosynthetic rate of Nerium oleander and Ricinus communis was 33.90% and 27.94% along R-1 and 41.85% and 32.409% along R-2 road, respectively. The decreased photosynthesis in roadside flora resulted in higher water use efficiency and substomatal CO2 concentration. However, higher antioxidant activity and free amino acid contents were noted in roadside plants that might be due to their defensive response to traffic pollutants. N. oleander was more affected by traffic pollutants and R. communis showed more resistance. Thus, N. oleander could be used for biomonitoring and R. communis for phytoremediation of vehicular pollution.

Spatial and Temporal Heterogeneity of Metal Contaminants in Soils Along Two Major Roads.

The concentrations of zinc, copper, lead and cadmium owing to vehicles were analyzed in roadside soils during all the four seasons from Faisalabad-Gojra road (M-4) and Gojra-Jhang road (GJR) in the Punjab, Pakistan. Results indicated that all the polluted sites along the roadside had comparatively higher metal concentrations as compared to control, nevertheless, spatio-temporal variations in metal concentrations were apparent in different soil samples. The spatial differences in metal concentrations were strongly associated with vehicle density at the Gojra Interchange site and Forest Park site along M-4 and GJR roads, respectively. Among seasons, metal ions exhibited the following order: summer > autumn > spring > winter, indicating that high temperature might have released more metal ions. The metals were recorded in contaminated soil in the order of Zn > Cu > Pb > Cd along both roads. This investigation emphasizes the need for proper environmental management particularly the improvement of roads and proper checking of vehicles running on these regards.

Lead Concentrations in Soils and Some Wild Plant Species Along Two Busy Roads in Pakistan.

This study assessed the level of Pb in soil and five wild plant species (Calotropis procera, Datura alba, Parthenium hysterophorus, Cenchrus ciliaris and Ricinus communis) during all the four seasons. Two busy roads varying in age and traffic volume were selected i.e., Faisalabad-Sargodha road (FSR) and Pindi Bhattian to Lillah (M-2) in the Punjab, Pakistan. Results showed raised levels of Pb in both plants and soil samples along both roads. The range of Pb concentration in plants was 0.08-3.98 and 1.95-4.74 mg kg- 1 for soil. Higher Pb contamination was recorded along FSR road as compared to M-2. Among seasons, the higher Pb concentration was found during summer, probably due to very high temperature. Among all the plants studied, Calotropis procera accumulated the highest level (3.98 mg kg- 1 dry wt.) of Pb; Thus, it can be used as good biomonitor/phytoremediator at Pb contaminated areas.