RESUMO
The majority of geomicrobiological research conducted on glacial systems to date has focused on glaciers that override primarily carbonate or granitic bedrock types, with little known of the processes that support microbial life in glacial systems overriding volcanic terrains (e.g., basalt or andesite). To better constrain the role of the supraglacial ecosystems in the carbon and nitrogen cycles, to gain insight into microbiome composition and function in alpine glacial systems overriding volcanic terrains, and to constrain potential elemental sequestration or release through weathering processes associated with snow algae communities, we examined the microbial community structure and primary productivity of snow algae communities on stratovolcanoes in the Cascade Range of the Pacific Northwest. Here, we present the first published values for carbon fixation rates of snow algae communities on glaciers in the Pacific Northwest. We observed varying levels of light-dependent carbon fixation on supraglacial and periglacial snowfields at Mt. Hood, Mt. Adams, and North Sister. Recovery of abundant 18S rRNA transcripts affiliated with photoautotrophs and 16S rRNA transcripts affiliated with heterotrophic bacteria is consistent with previous studies indicating the majority of primary productivity on snow and ice can be attributed to photoautotrophs. In contrast to previous observations of glacial ecosystems, our geochemical, isotopic, and microcosm data suggest these assemblages are not limited by phosphorus or fixed nitrogen availability. Furthermore, our data indicate these snow algae communities actively sequester Fe, Mn, and P leached from minerals sourced from the local rocks. Our observations of light-dependent primary productivity on snow are consistent with similar studies in polar ecosystems; however, our data may suggest that DIC may be a limiting nutrient in contrast to phosphorus or fixed nitrogen as has been observed in other glacial ecosystems. Our data underscore the need for similar studies on glacier surfaces and seasonal snowfields to better constrain the role of local bedrock and nutrient delivery on carbon fixation and biogeochemical cycling in these ecosystems.
Assuntos
Biota , Ciclo do Carbono , Microalgas/classificação , Microalgas/metabolismo , Neve/microbiologia , Bactérias/classificação , Análise por Conglomerados , DNA de Algas/química , DNA de Algas/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Noroeste dos Estados Unidos , Filogenia , RNA Ribossômico 16S/genética , RNA Ribossômico 18S/genética , Análise de Sequência de DNARESUMO
Little Salt Spring (Sarasota County, FL, USA) is a sinkhole with groundwater vents at ~77 m depth. The entire water column experiences sulfidic (~50 µM) conditions seasonally, resulting in a system poised between oxic and sulfidic conditions. Red pinnacle mats occupy the sediment-water interface in the sunlit upper basin of the sinkhole, and yielded 16S rRNA gene clones affiliated with Cyanobacteria, Chlorobi, and sulfate-reducing clades of Deltaproteobacteria. Nine bacteriochlorophyll e homologues and isorenieratene indicate contributions from Chlorobi, and abundant chlorophyll a and pheophytin a are consistent with the presence of Cyanobacteria. The red pinnacle mat contains hopanoids, including 2-methyl structures that have been interpreted as biomarkers for Cyanobacteria. A single sequence of hpnP, the gene required for methylation of hopanoids at the C-2 position, was recovered in both DNA and cDNA libraries from the red pinnacle mat. The hpnP sequence was most closely related to cyanobacterial hpnP sequences, implying that Cyanobacteria are a source of 2-methyl hopanoids present in the mat. The mats are capable of light-dependent primary productivity as evidenced by 13 C-bicarbonate photoassimilation. We also observed 13 C-bicarbonate photoassimilation in the presence of DCMU, an inhibitor of electron transfer to Photosystem II. Our results indicate that the mats carry out light-driven primary production in the absence of oxygen production-a mechanism that may have delayed the oxygenation of the Earth's oceans and atmosphere during the Proterozoic Eon. Furthermore, our observations of the production of 2-methyl hopanoids by Cyanobacteria under conditions of low oxygen and low light are consistent with the recovery of these structures from ancient black shales as well as their paucity in modern marine environments.
Assuntos
Processos Autotróficos , Chlorobium/metabolismo , Cianobactérias/metabolismo , Água Subterrânea/microbiologia , Processos Fototróficos , Aerobiose , Anaerobiose , Biomarcadores/análise , Florida , PaleontologiaRESUMO
The LMO2 gene is involved in T-cell acute leukaemia (T-ALL) in children with chromosomal translocations t(11;14)(p13;q11) or (7;11)(q35;p13). Transgenic expression of Lmo2 in T cells results in clonal tumours with long latency indicating that mutations in other genes are required for the development of overt tumours. RAG V-D-J recombinase can mediate genetic transposition and thus might create the secondary mutations necessary for T-ALL. Tumour development was compared in Lmo2 transgenic mice in the presence or absence of the Rag1 gene. No difference was observed in the rate of tumour formation nor in tumour histology in Lmo2-transgenic mice with or without Rag1. We conclude that, in this model, RAG recombinase is not a major mediator of mutations needed for T cell tumorigenesis and that antigen binding to alpha-beta or to gamma-delta T cell receptor does not play a role in tumorigenesis. The driving force behind the mutational process involved in this transgenic model remains obscure.
Assuntos
Proteínas de Ligação a DNA/genética , Proteínas de Homeodomínio/fisiologia , Leucemia-Linfoma de Células T do Adulto/etiologia , Metaloproteínas/genética , Proteínas Adaptadoras de Transdução de Sinal , Animais , Proteínas de Homeodomínio/genética , Proteínas com Domínio LIM , Leucemia-Linfoma de Células T do Adulto/genética , Leucemia-Linfoma de Células T do Adulto/patologia , Camundongos , Camundongos Knockout , Camundongos Transgênicos , Mutação , Timoma/etiologia , Timoma/patologiaRESUMO
Anorexia is a debilitating disorder which affects significant numbers of young women. Brumberg has suggested a causal relationship in young women between feminism and anorexia. In this study, traditional-aged female college students completed the Attitudes Toward Women Scale and the Eating Attitudes Test. The hypothesized relationship between feminism and anorexia was not found.
Assuntos
Anorexia Nervosa/psicologia , Identidade de Gênero , Direitos da Mulher , Adulto , Anorexia Nervosa/diagnóstico , Imagem Corporal , Feminino , Humanos , Inventário de PersonalidadeRESUMO
Mahoney Lake represents an extreme meromictic model system and is a valuable site for examining the organisms and processes that sustain photic zone euxinia (PZE). A single population of purple sulfur bacteria (PSB) living in a dense phototrophic plate in the chemocline is responsible for most of the primary production in Mahoney Lake. Here, we present metagenomic data from this phototrophic plate--including the genome of the major PSB, as obtained from both a highly enriched culture and from the metagenomic data--as well as evidence for multiple other taxa that contribute to the oxidative sulfur cycle and to sulfate reduction. The planktonic PSB is a member of the Chromatiaceae, here renamed Thiohalocapsa sp. strain ML1. It produces the carotenoid okenone, yet its closest relatives are benthic PSB isolates, a finding that may complicate the use of okenone (okenane) as a biomarker for ancient PZE. Favorable thermodynamics for non-phototrophic sulfide oxidation and sulfate reduction reactions also occur in the plate, and a suite of organisms capable of oxidizing and reducing sulfur is apparent in the metagenome. Fluctuating supplies of both reduced carbon and reduced sulfur to the chemocline may partly account for the diversity of both autotrophic and heterotrophic species. Collectively, the data demonstrate the physiological potential for maintaining complex sulfur and carbon cycles in an anoxic water column, driven by the input of exogenous organic matter. This is consistent with suggestions that high levels of oxygenic primary production maintain episodes of PZE in Earth's history and that such communities should support a diversity of sulfur cycle reactions.
Assuntos
Chromatiaceae/genética , Chromatiaceae/metabolismo , Lagos/microbiologia , Enxofre/metabolismo , Colúmbia Britânica , Genoma Bacteriano , Dados de Sequência Molecular , Oxirredução , Filogeografia , Análise de Sequência de DNARESUMO
Chlorophotoautotrophy, the use of chlorophylls to convert light energy into chemical energy for carbon dioxide fixation, is the primary metabolic process linking the inorganic and organic carbon pools on Earth. To understand the potential effects of various environmental constraints on the evolution of chlorophototrophy better, we studied the distribution, diversity, and abundance of chlorophylls and genes involved in their synthesis along geothermal gradients in Yellowstone National Park, Wyoming. Genes involved in chlorophyll biosynthesis were constrained to temperatures of less than ~70 °C and were only detected at this elevated temperature when the pH was in the circumneutral to alkaline range. The upper temperature limit for the detection of chlL/bchL(1) and bchY(2) decreased systematically with increasingly acidic pH, an observation likely attributable to sulfide, which upon oxidation, generates acidic spring water and reduces the availability of bicarbonate the preferred source of inorganic carbon for phototrophs. Spring pH was also the best predictor of the phylogenetic diversity of chlL/bchL communities. The phylogenetic similarity of chlL/bchL genes between sites was significantly correlated with that of chlorophylls. The predominance of chlorophyll a and bacteriochlorophyll a among extracted pigments was consistent with predominance of chlL/bchL genes affiliated with the Cyanobacteria and Chloroflexiales, respectively, and might be related to the fact that the majority of these organisms are photoautotrophs. Together, these results suggest that a combination of temperature, pH, and/or sulfide influences the distribution, diversity, and evolution of chlorophotrophs and the chlorophylls that they synthesize.
Assuntos
Biodiversidade , Clorofila/metabolismo , Cianobactérias/classificação , Cianobactérias/isolamento & purificação , Fontes Termais/microbiologia , Processos Fototróficos , Proteínas de Bactérias/genética , Clorofila/genética , Análise por Conglomerados , Cianobactérias/genética , Cianobactérias/fisiologia , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Metabolismo Energético , Genes Bacterianos , Variação Genética , Concentração de Íons de Hidrogênio , Dados de Sequência Molecular , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , WyomingRESUMO
Mounting evidence indicates the presence of a near complete biological nitrogen cycle in redox-stratified oceans during the late Archean to early Proterozoic (c. 2.5-2.0 Ga). It has been suggested that the iron (Fe)- or vanadium (V)-dependent nitrogenase rather than molybdenum (Mo)-dependent form was responsible for dinitrogen fixation during this time because oceans were depleted in Mo and rich in Fe. We evaluated this hypothesis by examining the phylogenetic relationships of proteins that are required for the biosynthesis of the active site cofactor of Mo-nitrogenase in relation to structural proteins required for Fe-, V- and Mo-nitrogenase. The results are highly suggestive that among extant nitrogen-fixing organisms for which genomic information exists, Mo-nitrogenase is unlikely to have been associated with the Last Universal Common Ancestor. Rather, the origin of Mo-nitrogenase can be traced to an ancestor of the anaerobic and hydrogenotrophic methanogens with acquisition in the bacterial domain via lateral gene transfer involving an anaerobic member of the Firmicutes. A comparison of substitution rates estimated for proteins required for the biosynthesis of the nitrogenase active site cofactor and for a set of paralogous proteins required for the biosynthesis of bacteriochlorophyll suggests that Nif emerged from a nitrogenase-like ancestor approximately 1.5-2.2 Ga. An origin and ensuing proliferation of Mo-nitrogenase under anoxic conditions would likely have occurred in an environment where anaerobic methanogens and Firmicutes coexisted and where Mo was at least episodically available, such as in a redox-stratified Proterozoic ocean basin.
Assuntos
Euryarchaeota/enzimologia , Evolução Molecular , Molibdênio/metabolismo , Ciclo do Nitrogênio , Nitrogenase/metabolismo , Proteínas de Bactérias/genética , Euryarchaeota/genética , Duplicação Gênica , Fusão Gênica , Fotossíntese , FilogeniaRESUMO
There are three major isoforms of BAG-1 in mammalian cells, termed BAG-1L (p50), BAG-1M (p46) and BAG-1S (p36) that function as pro-survival proteins and are associated with tumorigenesis and chemoresistance. Initiation of BAG-1 protein synthesis can occur by both cap-dependent and cap-independent mechanisms and it has been shown that synthesis of BAG-1S is dependent upon the presence of an internal ribosome entry segment (IRES) in the 5'-UTR of BAG-1 mRNA. We have shown previously that BAG-1 IRES-meditated initiation of translation requires two trans-acting factors poly (rC) binding protein 1 (PCBP1) and polypyrimidine tract binding protein (PTB) for function. The former protein allows BAG-1 IRES RNA to attain a structure that permits binding of the ribosome, while the latter protein appears to be involved in ribosome recruitment. Here, we show that the BAG-1 IRES maintains synthesis of BAG-1 protein following exposure of cells to the chemotoxic drug vincristine but not to cisplatin and that this is brought about, in part, by the relocalization of PTB and PCBP1 from the nucleus to the cytoplasm.
Assuntos
Proteínas de Ligação a DNA/biossíntese , Proteínas de Ligação a DNA/genética , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Estresse Oxidativo , RNA Mensageiro/metabolismo , Ribossomos/genética , Fatores de Transcrição/biossíntese , Fatores de Transcrição/genética , Cisplatino/toxicidade , Células HeLa , Humanos , Estresse Oxidativo/efeitos dos fármacos , RNA Mensageiro/genética , Ribossomos/efeitos dos fármacos , Moduladores de Tubulina/toxicidade , Vincristina/toxicidadeRESUMO
Upon cell-cycle arrest or nutrient deprivation, the cellular rate of ribosome production is reduced significantly. In mammalian cells, this effect is achieved in part through a co-ordinated inhibition of RP (ribosomal protein) synthesis. More specifically, translation initiation on RP mRNAs is inhibited. Translational regulation of RP synthesis is dependent on cis-elements within the 5'-UTRs (5'-untranslated regions) of the RP mRNAs. In particular, a highly conserved 5'-TOP (5'-terminal oligopyrimidine tract) appears to play a key role in the regulation of RP mRNA translation. This article explores recent developments in our understanding of the mechanism of TOP mRNA regulation, focusing on upstream signalling pathways and trans-acting factors, and highlighting some interesting observations which have come to light following the recent development of cDNA microarray technology coupled with polysome analysis.
Assuntos
Regiões 5' não Traduzidas , Biossíntese de Proteínas , Pirimidinas/metabolismo , Proteínas Ribossômicas/metabolismo , Animais , Regulação da Expressão Gênica , Humanos , Estrutura Molecular , Polirribossomos/genética , Polirribossomos/metabolismo , Capuzes de RNA/química , Capuzes de RNA/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Proteínas Ribossômicas/genética , Transdução de Sinais/fisiologiaRESUMO
Various methods have been proposed and investigated for the purpose of reducing radiation exposures in cephalometric radiography. The purpose of this investigation was to use various dose-reducing methods from four major categories--(1) rare-earth intensifying screens, (2) rare-earth filtration, (3) prepatient soft-tissue enhancement methods, and (4) films varying in speed and latitude--to determine optimal combinations on the basis of exposure reduction and image quality achieved. In laboratory tests and clinical trials, radiation doses were compared along with various tests of image quality for the standard system currently used at this institution and for experimental systems. Image quality was assessed by standard panel-of-expert methods and more quantitative methods involving optical densitometry, contrast indices, and landmark envelopes of error. Results demonstrated that significant exposure reductions were achievable, often with improved image quality. The degree of exposure reduction was dependent largely upon the type of intensifying screen and to a lesser extent on beam filtration or film types. The greatest reduction in exposures were achieved with techniques using rare-earth beam filtration. Image quality was highest with a new wide-latitude type film. Because such significant reduction in radiation exposure for cephalometrics is possible with new techniques, we conclude that a change from the previous standard should occur. In establishing a new technique, orthodontists should consider adopting rare-earth screens and beam filters, flat-grained films, prepatient soft-tissue enhancement methods, and the elimination of grids.