Potential bio-functional properties of Citrus aurantium L. leaf: chemical composition, antiviral activity on herpes simplex virus type-1, antiproliferative effects on human lung and colon cancer cells and oxidative protection.

This study examined the antioxidant, anticancer and antiviral properties of the methanolic extracts from bigarade (Citrus aurantium L.) leaves at two development stages. Ferulic acid, naringin and naringenin were the principal phenolic components of young and old leaves. The highest total antioxidant capacity was obtained in young leaf extracts (YLE). These latter also exhibited the highest antiradical DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) activities, while the highest iron chelating and reducing power activities were observed in old leaf extracts (OLE). The potent anticancer activity was observed in YLE for human lung carcinoma (A-549) and in OLE for colon adenocarcinoma (DLD-1) cells. YLE showed the highest virucidal effects as compared to OLE and the positive control acyclovir against herpes simplex virus type-1 (HSV-1) propagation in Vero cells during the absorption and replication periods. The young and old leaves might be a source of natural antioxidants and protective agents against oxidative damage.

Tetraclinis articulata essential oil emulsion use as alternative to chemical fungicide to control tomato grey mould disease.

Tetraclinis articulata essential oil proved to be effective in controlling tomato grey mould, so we would investigate its effect on some tomato defense mechanisms. The pretreatment of Botrytis cinerea infected tomato plants with TAEO emulsion enhanced the activity of antioxidant enzymes activity SOD, CAT, APX, and GPX, and total polyphenols content and it decreased IC50 of free radical-scavenging activity and H2O2 content. Results showed amelioration in antioxidant status in TAEO emulsion treated and B. cinerea infected plants indicating that treatment decreased infection in tomato plants. The qRT-PCR analysis of defense genes expression Chitinase SlChi, transcription factors SlWRKY and SlAP2/ERF, Lipoxygenase SlLOX, and Thioredoxin SlTRX showed that they were up-regulated as early as 12 hpi sustained with a second increase at 48 hpi in TAEO emulsion pretreated and infected plants. These results suggest the potential use of TAEO emulsion as natural product to induce tomato antioxidant status and activate defense genes.

Optimizing prickly pear by-product valorization: formulating molasses with enhanced antioxidant capacities and sugar contents.

This study endeavoured to capitalize on prickly pear by-products for the optimization of molasses formulation, targeting elevated antioxidant capacities and superior sugar contents. Through robust statistical modelling, the optimal cooking parameters-temperature (70-80 °C) and duration (60-90 min)-were determined, guided by responses of antioxidant activity and Brix value. A D-Optimal mixture design further delineated the ideal proportions of molasses components (pulp, peel, and seeds). Characterization revealed that peel harboured the highest concentrations of total polyphenols (396.41 mg GAE/100g FW) and flavonoids (234.26 mg CE/100g FW), emphasizing its antioxidant potential (DPPH inhibition IC50: 12.72 µg/ml). The optimal cooking conditions were established at 78.35 °C for 79.70 min, with predictive equations guiding ingredient proportions (0.265 g pulp, 0.710 g peel, 0.025 g seed). Intriguingly, while peel inclusion enhanced total sugar content and antioxidant activity, seed incorporation exerted a contrasting effect by reducing total sugar content and limiting antioxidant activity.

Chemical profiling of maceration and decoction of Tamarix gallica L. organs and in vitro biological properties.

Tamarix gallica L. is a halophytic medicinal shrub traditionally used in the treatment of liver disorders. Leaf and flower infusions are widely used for anti-diarrheic and anti-inflammatory preparations. In this work, we have explored the combined effect of method (Maceration and Decoction) and solvent extraction (70% Methanol, 70% Ethanol, 70% Acetone, and Water) on phenolic composition and biological activities (antioxidant and anti-inflammatory) of different T. gallica organ extracts (Leaves, Flowers, Stems, and Fruits). Obtained results showed that Tamarix leaves reveal more potent antioxidant activity. Methanol (70%) was the best maceration solvent for the extraction of leaves and flowers with high total antioxidant and anti-radical capacities. HPLC analysis showed that catechin, isorhamnetin-3-O-glucoside, isoquercetin were the major phenolics in 70% methanolic extract. Furthermore, this extract showed considerable anti-inflammatory activity. This prospect could be of great importance in the valuation of this halophyte as a source of natural antioxidants and anti-inflammatory drugs.

Bioassay-guided isolation, identification and activity evaluation of antifungal compounds from Cupressus sempervirens.

The quest for eco-friendly antifungal compounds from natural sources has surged, seeking alternatives to synthetic fungicides. In this study, we explored Cupressus sempervirens organic extracts antifungal potential against Botrytis cinerea, a destructive fungus causing grey mold disease in crops. Extracts from various phenological stages were evaluated for their antifungal activities. The dichloromethanolic extract from the flowering stage exhibited the highest efficacy, completely inhibiting B. cinerea mycelial growth, at 250 µg/mL and preventing conidia germination at 500 µg/mL. Bioguided fractionation and chromatography, led to the identification of isoquercetin as the active compound responsible for the antifungal effects. These findings present promising possibilities for the development of sustainable biofungicides to combat grey mould disease in agriculture. Further investigations into isoquercetin's potential as a biofungicide are warranted.

Exploring the synergistic potential of wild nettle and olive oil: bioactive compounds, antioxidant capacity, and antibacterial properties.

This study delved into the incorporation of wild nettle (Urtica dioica L.) leaves powder with virgin olive oil, exploring its potential to enhance nutritional value and health benefits. The antioxidant, antibacterial, and mineral-enrichment attributes of the resultant nettle-infused olive oil were investigated. Phenolic composition analysis revealed that an optimal maceration time of 30-40 minutes leads to maximal phenolic and flavonoid contents (57.66 mg GAE/100 g and 67.18 mg QE/100 g, respectively). The infused oil demonstrated superior radical scavenging activity (DPPH and ABTS assays) compared to virgin olive oil. Notably, nettle infusion significantly elevates zinc (10.12 mg/kg) and iron (13.85 mg/kg) contents. Antibacterial assays exhibited enhanced inhibition diameters and lower MIC and MBC values for nettle-infused oil against various bacterial strains. The study's findings underscore the potential of nettle infusion to enhance the nutritional and functional attributes of olive oil, holding promise for novel culinary and health applications.

Essential oil and its nanoemulsion of Eucalyptus cladocalyx: chemical characterization, antioxidant, anti-inflammatory and anticancer activities.

Formulating a nanoemulsion (NE) of essential oil (EO) could enhance its efficiency while requiring lower concentrations. Eucalyptus cladocalyx F. Muell EO was rich in monoterpenes hydrocarbons. NE was prepared and the effect of surfactant (Tween 20, 40 and 80) and shearing time were investigated. The results showed that the best NE was formed using Tween 80 after 25 min of emulsification. Small droplet size (40 nm), low polydispersity index PDI (0.49), and stable zeta potential highlighted the excellent NE stability which was tested under storage conditions for 4 months. The results showed that the antioxidant and anticancer activities of NE were enhanced compared to free EO. Furthermore, NE and EO exhibited high anti-inflammatory effects by inhibiting nitric oxide (NO), Interleukin 6 (IL-6), and tumor necrosis factors alpha (TNF-α) production in liposaccharides (LPS)-induced RAW264.7 cells. In conclusion, a stable Eucalyptus cladocalyx-NE was produced, with improved biological activities.

Enhancing antioxidant and antibacterial properties of olive oil through garlic enrichment: a comprehensive study.

The amalgamation of garlic's antibacterial potency with olive oil's nutritional benefits provides a natural, effective way to boost health and counters microbial threats. This study explored the antioxidant and antibacterial traits of garlic-enriched virgin olive oil (VOO) samples, focusing on various garlic forms (fresh, oven-dried, freeze-dried). Comparative analysis revealed fresh garlic's highest total phenolic content, flavonoid content, and strongest DPPH scavenging activity. GC/MS analysis unveiled distinct volatile profiles. Fresh garlic oil contained elevated allyl-methy-sulfide, diallyl-trisulfide, methyl-propyl-disulfide levels. Antibacterial evaluation displayed substantial inhibition zones, especially fresh garlic oil against E. coli, and oven-dried/freeze-dried garlic oils against P. aeruginosa. Lower Minimal Inhibitory Concentration (MIC) values for fresh garlic oil and freeze-dried garlic oil against both Gram-negative and Gram-positive bacteria signify potent antibacterial activity of garlic-enriched VOO. These findings underscore garlic-enriched VOO's potential as natural antibacterial agents, fortified with antioxidant traits, while emphasizing drying methods' role in shaping volatile compounds.

Biological control of Citrus brown spot pathogen, "Alternaria alternata" by different essential oils.

The antifungal effects of laurel, myrtle and peppermint essential oils and their combinations were investigated in vitro on two strains of Alternaria alternata mycelial growth and in vivo on detached Citrus leaf disease incidence. Myrtle essential oil was rich in α-pinene and 1,8-cineole while peppermint essential oil in menthol and menthone. 1,8-Cineole was the main component of the essential oils from laurel, laurel + myrtle and peppermint + laurel. The combined peppermint and myrtle essential oil was characterized by the predominance of menthol and 1,8-cineole. All tested essential oils, incorporated in potato-dextrose agar, inhibited A. alternata mycelial growth and had a fungistatic effect at concentration 3 mg/mL of medium. A great synergism was detected between peppermint and laurel essential oils against the two strains of A. alternata. The combined laurel and peppermint essential oil reduced mycelial growth rates of inoculated detached leaves at concentration above 1.5 mg/mL.

Laurel essential oil: biological activities and application for semolina preservation against the red flour beetle Tribolium castaneum (Tenebrionidae).

The phytochemical composition of Laurus nobilis essential oil and their anticholinesterase, antioxidant, and insecticidal potential were studied. Also, the oil volatile fraction was compared in semolina at the beginning and after storage periods. For that, a headspace solid-phase-microextraction analysis (HS-SPME) coupled with gas chromatography and mass spectrometry was undertaken. Significant quantitative and qualitative differences of the oil volatile fraction were detected according to storage periods and occupation space ratios. Additionally, anti-acetylcholinesterase activity of L. nobilis oil against T. castaneum adults was evaluated. Results revealed that insecticide activity varied according to storage duration and occupation space. Besides, the oil acts on acetylcholine hydrolysis by inhibiting the activity of acetylcholinesterase. These results highlighted that L. nobilis essential oil may be recommended as an eco-friendly alternative for preserving semolina during storage.

Phytochemical analysis, antioxidant, anticancer and anti-inflammatory activities of Lycium europaeum fruits.

Lycium europaeum is used as a medicinal herb in many countries. In this study, cyclohexane, dichloromethane, ethyl acetate, methanol and water were used as solvents in the extraction of L. europaeum fruits. The contents of total phenolics, total flavonoids, total tannins and condensed tannins as well as the biological activities of these extracts were investigated using various in vitro and ex vivo assays. Results showed that all solvent extracts of L. europaeum had no anticancer activity against cancerous (A-549 and DLD-1) and non-cancerous (WS-1) human cells. Methanol and ethyl acetate were the most effective solvent for extraction of phenolic compounds and also exhibited the highest antioxidant and anti-inflammatory activities. The methanol extract of L. europaeum fruits was the richest in phenolic compounds with the predominance of ferulic acid, catechin and narengin. These results supported the use of L. europaeum fruit as natural source of bioactive compound for pharmaceutical applications.

Essential Oil Stabilisation by Response Surface Methodology (RSM): Nanoemulsion Formulation, Physicochemical, Microbiological, and Sensory Investigations.

This manuscript aimed to optimise the encapsulation of Thymus capitatus essential oil into nanoemulsion. Response Surface Methodology results were best fitted into polynomial models with regression coefficient values of more than 0.95. The optimal nanoemulsion showed nanometer-sized droplets (380 nm), a polydispersity index less than 0.5, and a suitable Zeta potential (-10.3 mV). Stability results showed that nanoemulsions stored at 4 °C were stable with the lowest d3,2, PolyDispersity Index (PDI), and pH (day 11). Significant ameliorations in the capacity to neutralise DPPH radical after the encapsulation of the antimicrobial efficacy of thyme essential oil were recorded. S. typhimurium growth inhibition generated by nanoencapsulated thyme essential oil was 17 times higher than by bulk essential oil. The sensory analysis highlighted that the encapsulation of thyme essential oil improved enriched milk's sensory appreciation. Indeed, 20% of the total population attributed a score of 4 and 5 on the scale used for milk enriched with nanoemulsion. In comparison, only 11% attributed the same score to milk enriched with bulk essential oil. The novel nanometric delivery system presents significant interest for agroalimentary industries.

Bioguided fractionation of procyanidin B2 as potent anti coxsackie virus B and Herpes simplex from cypress (Cupressus sempervirens L.).

The methanolic extracts of cypress (Cupressus sempervirens L.) collected at three phenological stages were evaluated for their cytotoxicity on Vero cells by MTT test as well as on Herpes simplex (HSV-2) and coxsackie (CVB-3) viruses by plaque reduction assay. The methanolic extract exhibited the highest cytotoxicity against HSV-2 (IC50 = 20.40 µg/mL) and CVB-3 (IC50 = 47.50 µg/mL) at the flowering stage. This extract also exhibited a virucidal action both during the entry of viruses and the release of newly formed virions. The methanolic extract bioguided purification showed that the ethyl-acetate fraction was responsible for virucidal activity. This fraction was endowed with more important selectivity index of 8.15 for HSV-2 and 4.40 for CVB-3. The ethyl acetate fraction was subjected to thin layer chromatography fractionation and identification by HPLC-DAD-ESI-MSn. Results showed that the condensed tannin procyanidin B2 was identified for the first time responsible of the antiviral activity of cypress.

Natural colourants analysis and biological activities. Association to molecular markers to explore the biodiversity of Opuntia species.

INTRODUCTION: Synthetic colourants are subject to various allergies. As a result, the use of plant-based additives has proved promising. OBJECTIVE: It is in this perspective that our objective was undertaken to investigate natural colourants from edible cactus. Opuntia species populations were single-sequence repeat (SSR) fingerprinted, the analysis of betalains and phenolic compounds and the evaluation of their biological potentials were also characterised. RESULTS: Photometric quantification of betalains and phenols showed an interspecific variation across Opuntia species. Opuntia ficus indica fruits showed the highest betalain [betaxanthins; 843.67 and betacyanins; 1400 mg/100 g dry weight (DW)] and polyphenol contents. Reversed-phase high-performance liquid chromatography (RP-HPLC) analysis showed that the variation of individual phenols profile was influenced by interspecific and genetic factors. Isorhamnetin-O-(di-deoxyhexosyl-hexoside) was the major compound and its content varied according to Opuntia species, while catechol was the predominant phenolic compound in O. humifusa with 1.88 µg/g DW. Concerning cactus species, Opuntia colourants exhibited a potent antiradical activity [half maximal inhibitory concentration (IC50 ) up to 1 µg/mL]. Opuntia species were effective against Gram-positive and Gram-negative bacterial strains [inhibition zone (IZ) up to 27 mm]. A high genetic diversity within Opuntia genotypes based on SSR markers was revealed. UPGMA (unweighted pair group method with arithmetic mean) dendrogram and PCoA (principal coordinate analysis) based on natural pigments and antimicrobial profiles indicated significant variation. The correlation approach proved the presence of a probably metabolic relationship between genetic markers, pigments and their biological activities. CONCLUSION: A possible association between molecular approach and metabolic profile analysis of Opuntia allows tracing the relationship among species for its genetic conservation.

Anti HSV-2 activity of Peganum harmala (L.) and isolation of the active compound.

Genital herpes is a sexually transmitted disease caused by herpes simplex virus type 2 (HSV-2). Nucleoside analogues such as acyclovir (ACV) are the usual therapy for treating HSV infection. However, the overuse of this drug has led to the emergence of resistant strains. Therefore, the search for new alternative or complementary molecules to overcome this obstacle is needed. In this objective, Peganum harmala was investigated for its HSV-2 activity. The organic extracts of the different plant organs were evaluated for their cytotoxicity on Vero cells by the MTT test and anti HSV-2 activity by plaque reduction assay. Only the methanol seeds extract was active with a 50% inhibitory concentration (IC50) and a selectivity index (SI) of 161 and 13.2 µg/mL, respectively. In addition, the study of the antiviral mode of action revealed that this extract exerts a virucidal action both during the entry of viruses and the release of the newly formed virions, whereas no cell protection effect was observed. The active compound was isolated by bio-guided purification using thin layer chromatography (TLC) and identified by GC-MS and HPLC-DAD-ESI-MSn as harmine. The combination of harmine standard compound with ACV showed a combination index (CI) of 0.5 indicating that these two compounds have a synergic effect. This data suggests that harmine could be associated to ACV to improve the treatment of genital herpes essentially for the immunocompromised patients.

Antifungal mechanism of the combination of Cinnamomum verum and Pelargonium graveolens essential oils with fluconazole against pathogenic Candida strains.

The present study aimed to investigate the anti-Candida activity of ten essential oils (EOs) and to evaluate their potential synergism with conventional drugs. The effect on secreted aspartic protease (SAP) activity and the mechanism of action were also explored. The antifungal properties of essential oils were investigated using standard micro-broth dilution assay. Only Cinnamomum verum, Thymus capitatus, Syzygium aromaticum, and Pelargonium graveolens exhibited a broad spectrum of activity against a variety of pathogenic Candida strains. Chemical composition of active essential oils was performed by gas chromatography-mass spectrometry (GC-MS). Synergistic effect was observed with the combinations C. verum/fluconazole and P. graveolens/fluconazole, with FIC value 0.37. Investigation of the mechanism of action revealed that C. verum EO reduced the quantity of ergosterol to 83%. A total inhibition was observed for the combination C. verum/fluconazole. However, P. graveolens EO may disturb the permeability barrier of the fungal cell wall. An increase of MIC values of P. graveolens EO and the combination with fluconazole was observed with osmoprotectants (sorbitol and PEG6000). Furthermore, the combination with fluconazole may affect ergosterol biosynthesis and disturb fatty acid homeostasis in C. albicans cells as the quantity of ergosterol and oleic acid was reduced to 52.33 and 72%, respectively. The combination of P. graveolens and C. verum EOs with fluconazole inhibited 78.31 and 64.72% SAP activity, respectively. To our knowledge, this is the first report underlying the mechanism of action and the inhibitory effect of SAP activity of essential oils in synergy with fluconazole. Naturally occurring phytochemicals C. verum and P. graveolens could be effective candidate to enhance the efficacy of fluconazole-based therapy of C. albicans infections.

Chemical Composition and Behavioral Effects of Five Plant Essential Oils on the Green Pea Aphid Acyrthosiphon pisum (Harris) (Homoptera: Aphididae).

Essential oils (EOs) from Schinus molle, Helichrysum gymnocephalum, Cedrelopsis grevei and Melaleuca viridiflora, four aromatic and medicinal plants, are commonly used in folk medicine. EOs were characterized by gas chromatography/mass spectrometry (GC/MS) and quantified by gas chromatography-flame ionization detection (GC-FID); then evaluated for their behavioral effects on adults of the green pea aphid Acyrthosiphon pisum (Harris) using a Perspex four-armed olfactometer in order to test the compatibility of their use as phytoinsecticides to control this insect pest. Our results showed that the EOs from the leaves of S. molle, M. viridiflora and C. grevei did not change aphids' behavior. However, S. molle fruits EO seemed to be attractive while H. gymnocephalum leaves EO exhibited repellency towards aphids at a dose of 10 µl. The major compounds in S. molle fruits EO were 6-epi-shyobunol (16.22%) and d-limonene (15.35%). While, in H. gymnocephalum leaves EO, 1,8-cineole was the main compound (47.4%). The difference in aphids' responses to these two EOs could be attributed to the differences in their compositions. Our findings suggest that these two EOs have potential applications for the integrated pest management of A. pisum (Harris).

Antifungal activity of volatile compounds-producing Pseudomonas P2 strain against Rhizoctonia solani.

Several volatile organic compounds (VOCs) producing endophyte bacteria were isolated from the leaves of olive trees and tested for their antifungal activity against several pathogenic fungi. An antagonistic strain called P2 showed 97 % of homology with Pseudomonas sp. strains on the basis of its 16S rDNA sequence and biochemical properties. P2 strain drastically inhibited the growth of Rhizoctonia solani mycelia (86 %) at 5 day-post-confrontation (dpc) and strongly reduced fungi infection on potato slices at 10(7) bacteria ml(-1) for 3 and 7 dpc. P2 strain was also positive for protease activity as well as siderophore production. Light microscopy analysis showed that treatment of R. solani mycelia with P2 strain induced thickening of the cell-wall, vesiculation of protoplasm and blockage of fungal hyphae branching. VOCs analysis using GC-MS allowed the detection of two major products with m/z of 93.9910 and 125.9630 corresponding to dimethyl disulfide and dimethyl trisulfide respectively. VOCs-producing P2 strain could be a promising agent in the protection of tuber crops against fungal diseases.

Formulation of pasta enriched with protein-rich lupine (Lupinus mutabilis Sweet) and wheat bran using mixture design approach.

In recent years, as awareness of healthy diets has increased, consumers are becoming more aware of their food and are paying more attention to their diet. Nutritionists have recommended an increased consumption of dietary fiber in the daily diet to improve health. Within this context, this work aims to formulate "spaghetti" pasta enriched with lupin flour and wheat bran. For the formulation of the mixture of those flours, we used the NemrodW software to predict the optimal pasta formula. A physicochemical characterization, as well as the culinary quality of the pasta resulting from the different mixtures, was carried out to model each significant criterion and choose the optimal mixture that will be used in the pasta recipe. The evaluation of the physicochemical characteristics of the pasta showed that the addition of lupin flour and bran resulted in an improvement in the nutritional value of the enriched spaghetti. Following the results obtained, the software proposed an optimal mix and will be used for further study. This formula comprises 19.60% bran, 27.83% lupin flour, and 52.75% durum wheat semolina. The caloric value of the dough from the optimal mix was determined and compared to that of the standard dough. The results of our study indicated a significant improvement in the nutritional value of the enriched pasta compared to the standard pasta. The enriched pasta contained higher levels of protein, ash, lipids, polyphenols, and pigments and lower levels of sugars, which make it a more nutritious option for certain individuals, such as athletes or those following a protein or low-sugar diet. PRACTICAL APPLICATION: The proposed method of substituting wheat semolina with lupine flour and wheat bran in spaghetti formulation has demonstrated a potential for producing protein-rich and nutritionally enriched pasta. This approach can be useful for developing similar functional food products that cater to the dietary requirements of athletes or individuals following a protein or low-sugar diet.

Comparative chemical composition and antioxidant activity of olive leaves Olea europaea L. of Tunisian and Algerian varieties.

The main objective of this research was to understand the effect of different solvents (ethanol, ethyl acetate, and hexane) on the extraction of bioactive compounds in addition to the antioxidant activity of eight Tunisian and Algerian olive leaf (Olea europaea L) cultivars. The different extracts were evaluated for their content of total phenols, flavonoids, and condensed tannins. The antioxidant capacity was determined by ABTS (2,2-azinobis (3 ethylbenzothiazoline-6-sulfonic acid) and DPPH (2,2-diphenyl-1 picrylhydrazyl) methods. In addition, qualitative and quantitative analyses of phenolic compounds by HPLC were performed. In terms of phenolic content, ethanol was found to be the best solvent to extract phenolic compounds with antioxidant capacity. The ethanolic extract of Sigoise and Rougette varieties were the richest in total phenols 161.54 ± 0.99, and 160.53 ± 1.17 mg GAE/g DE respectively. The highest levels of flavonoids were detected in ethyl acetate extracts of Rougette (87.58 ± 6.99 mg QE/g DE) and Verdal varieties (86.77 ± 1.75 mg QE/g DE), whereas the hexanolic extracts of Gerboui, Sofiana, and Chemlali exhibited the higher amount of tannins (67.99 ± 5.41, 65.52 ± 1.94, 64.64 ± 1.51 mg CE/g DE, respectively). Furthermore, in the DPPH radical scavenging activity assay, the Sofiana extract exhibited the highest antioxidant activity (23.85 ± 0.58 mg/L). In another case, for the ABTS radical scavenging assay, the Verdal extract showed high antioxidant potential (30.22 ± 1.35 mg/L). HPLC analysis of ethanolic extracts of the different varieties allowed the identification of 19 organic compounds, with oleuropein (2604.01-7991.14 mg/100g DM) being the most abundant, followed by hydroxytyrosol (250.74-687.54 mg/100g DM) and verbascoside (216.12-672.64 mg/100g DM) being the major compounds in the olive leaves in all studied ethanolic extracts, with concentrations varying significantly according to the cultivars and geographical origin. Principal component analysis (PCA) performed on phenolic profiles and antioxidant activities revealed the influence of bioactive compounds on antioxidant activity, with high concentrations of phenols and flavonoids in olive leaf extracts having a strong antioxidant activity.