Evaluation of medicinal plants using laser-induced breakdown spectroscopy (LIBS) combined with chemometric techniques.

Medicinal plants play a vital role in herbal medical field and allopathic medicine field industry. Chemical and spectroscopic studies of Taraxacum officinale, Hyoscyamus niger, Ajuga bracteosa, Elaeagnus angustifolia, Camellia sinensis, and Berberis lyceum are conducted in this paper by using a 532-nm Nd:YAG laser in an open air environment. These medicinal plant's leaves, roots, seed, and flowers are used to treat a range of diseases by the locals. It is crucial to be able to distinguish between beneficial and detrimental metal elements in these plants. We demonstrated how various elements are categorized and how roots, leaves, seeds and flowers of same plants differ from each other on the basis of elemental analysis. Furthermore, for classification purpose, different classification models, partial least square discriminant analysis (PLS-DA), k-nearest neighbors (kNN), and principal component analysis (PCA) are used. We found silicon (Si), aluminum (Al), iron (Fe), copper (Cu), calcium (Ca), magnesium (Mg), sodium (Na), potassium (K), manganese (Mn), phosphorous (P), and vanadium (V) in all of the medicinal plant samples with a molecular form of carbon and nitrogen band. We detected Ca, Mg, Si, and P as primary components in all of the plant samples, as well as V, Fe, Mn, Al, and Ti as essential medicinal metals, and additional trace elements like Si, Sr, and Al. The result's findings show that the PLS-DA classification model with single normal variate (SNV) preprocessing method is the most effective classification model for different types of plant samples. The average correct classification rate obtained for PLS-DA with SNV is 95%. Moreover, laser-induced breakdown spectroscopy (LIBS) was successfully employed to perform rapid, sensitive, and quantitative trace element analysis on medicinal herbs and plant samples.

Analysis of Different Techniques of Tonsillectomy: An Insight.

Tonsillectomy is one of the most commonly performed surgical procedure in otolaryngology especially in children. This is an age old procedure which has seen continuous changes in the surgical technique from guillotine method to snare technique to coblation tonsillectomy, and is still evolving day by day. But there are no consensus as to which technique is the best or most appropriate for tonsillectomy. The objective of this study is to compare three different surgical techniques of tonsillectomy namely the Cold dissection snare technique (CDST), Bipolar electro-dissection technique (BEDT) and Harmonic scalpel technique (HST) and to identify the method which is safe, with less operative time, which offers decreased intra-operative blood loss and with lowest post-operative morbidity and complications. This prospective and comparative study was conducted over a time duration of 1 year 6 months from January 2018 to July 2019 after the approval from ethical committee. Total 150 cases of tonsillectomy were done by dividing into three groups of 50 cases each. The study showed maximum cases of tonsillitis in the age group less than 10 years and the most common indication for tonsillectomy being chronic recurrent tonsillitis. Harmonic scalpel technique (HST) had least operative time, least intra-operative blood loss, took minimum time for resumption of normal diet and normal activity and also had least pain score on post-operative day 1st, 5th, 10th and 15th. STATISTICS: Kruskall-Wallis and the non-parametric Analysis of variance (ANOVA) tests were applied to determine statistically significant variances. All the differences are found to be significant P < 0.05. Harmonic Scalpel Technique (HST) is the latest technique as it is associated with quicker procedure, less intraoperative blood loss and less post-operative pain.

Extra-Rhino Cerebral Manifestations of Mucormycosis in Head and Neck Region: An Insight.

Mucormycosis is a potentially life-threatening fungal infection with a high mortality rate. The difficulty and delay in diagnosis due to its rarity usually results in a poor prognosis. Most common site in head and neck region is the nose and paranasal sinuses. However there are other very unusual areas in head and neck region where mucormycosis is encountered. Knowledge of these unusual areas is must and can save a patient's life. To elaborate and highlight the unusual areas in head and neck region where mucormycosis can mimic other common diseases. This retrospective study was done from May 2010 to May 2019 over a period of 9 years. All histopathologically confirmed cases of Head and Neck mucormycosis were evaluated and data analyzed. Total 35 cases of head neck mucormycosis were encountered from May 2010 to May 2019 over a period of 9 years. Out of which 30 cases (85.72%) were of rhino-orbito-cerebral mucormycosis and 5 cases (14.28%) were EXTRA rhino-orbito-cerebral mucormycosis in head neck region. Mucormycosis at such unusual sites can cause diagnostic dilemma for the treating doctor. Clinical knowledge with anticipation is a must for success in treatment of mucormycosis in unusual places in head neck region especially when normal looking diseases does not respond to usual treatment.