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1.
J Biol Chem ; 286(52): 44521-31, 2011 Dec 30.
Artigo em Inglês | MEDLINE | ID: mdl-22086927

RESUMO

During an inflammatory response, resident and newly recruited tissue macrophages adhere to extracellular matrix and cell-bound integrin ligands. This interaction induces the expression of pro-inflammatory mediators that include matrix metalloproteinases (MMPs). Arhgef1 is an intracellular signaling molecule expressed by myeloid cells that normally attenuates murine macrophage MMP production in vivo and in vitro after cell culture on the extracellular matrix protein, fibronectin. In this study, we have extended the characterization of this fibronectin-induced Arhgef1-regulated signaling pathway in both human and murine myeloid cells. Our results show that MMP9 production by fibronectin-stimulated monocytes and macrophages depends on autocrine thromboxane receptor signaling and that under normal conditions, this signaling pathway is attenuated by Arhgef1. Finally, we show that the expression of ARHGEF1 by human peripheral blood monocytes varies between individuals and inversely correlates with fibronectin-mediated MMP9 production.


Assuntos
Fibronectinas/metabolismo , Fatores de Troca do Nucleotídeo Guanina/metabolismo , Macrófagos Alveolares/metabolismo , Macrófagos Peritoneais/metabolismo , Metaloproteinase 9 da Matriz/biossíntese , Receptores de Tromboxanos/metabolismo , Transdução de Sinais/fisiologia , Animais , Comunicação Autócrina/fisiologia , Indução Enzimática/fisiologia , Humanos , Macrófagos Alveolares/citologia , Macrófagos Peritoneais/citologia , Camundongos , Fatores de Troca de Nucleotídeo Guanina Rho
2.
Am J Pathol ; 176(3): 1157-68, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20093499

RESUMO

Pulmonary immunity depends on the ability of leukocytes to neutralize potentially harmful and frequent insults to the lung, and appropriate regulation of leukocyte migration and adhesion is integral to this process. Arhgef1 is a hematopoietic-restricted signaling molecule that regulates leukocyte migration and integrin-mediated adhesion. To explore a possible regulatory role for Arhgef1 in pulmonary immunity we examined the lung and its leukocytes in wild-type and Arhgef1-deficient animals. Here we report that the lungs of Arhgef1-/- mice harbored significantly more leukocytes, increased expression and activity of matrix metalloproteinases (MMPs), airspace enlargement, and decreased lung elastance compared with wild-type lungs. Transfer of Arhgef1-/- lung leukocytes to wild-type mice led to airspace enlargement and impaired lung function, indicating that loss of Arhgef1 in leukocytes was sufficient to induce pulmonary pathology. Furthermore, we showed that Arhgef1-deficient peritoneal macrophages when either injected into the lungs of wild-type mice or cultured on fibronectin significantly increased expression and activity of MMPs relative to control macrophages, and the in vitro fibronectin induction was dependent on the alpha5beta1 integrin pair. Together these data demonstrate that Arhgef1 regulates alpha5beta1-mediated MMP expression by macrophages and that loss of Arhgef1 by leukocytes leads to pulmonary pathology.


Assuntos
Fatores de Troca do Nucleotídeo Guanina/metabolismo , Homeostase/imunologia , Imunidade/imunologia , Integrina alfa5beta1/metabolismo , Pulmão/enzimologia , Pulmão/imunologia , Metaloproteinases da Matriz/genética , Proteínas Proto-Oncogênicas/metabolismo , Animais , Fenômenos Biomecânicos/efeitos dos fármacos , Líquido da Lavagem Broncoalveolar/citologia , Células Cultivadas , Fibronectinas/farmacologia , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Fatores de Troca do Nucleotídeo Guanina/deficiência , Homeostase/efeitos dos fármacos , Imunidade/efeitos dos fármacos , Contagem de Leucócitos , Leucócitos/efeitos dos fármacos , Leucócitos/enzimologia , Leucócitos/patologia , Ligantes , Pulmão/patologia , Pulmão/fisiopatologia , Macrófagos Peritoneais/efeitos dos fármacos , Macrófagos Peritoneais/enzimologia , Macrófagos Peritoneais/patologia , Metaloproteinases da Matriz/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Proteínas Proto-Oncogênicas/deficiência , Testes de Função Respiratória , Fatores de Troca de Nucleotídeo Guanina Rho , Transdução de Sinais/efeitos dos fármacos
3.
J Immunol ; 182(12): 7430-9, 2009 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-19494266

RESUMO

Mast cells are important sentinels guarding the interface between the environment and the body: a breach in the integrity of this interface can lead to the release of a plethora of mediators that engage the foreign agent, recruit leukocytes, and initiate adaptive physiological changes in the organism. While these capabilities make mast cells critical players in immune defense, it also makes them important contributors to the pathogenesis of diseases such as asthma. Mast cell mediators induce dramatic changes in smooth muscle physiology, and the expression of receptors for these factors by smooth muscle suggests that they act directly to initiate constriction. Contrary to this view, we show herein that mast cell-mediated bronchoconstriction is observed only in animals with intact innervation of the lung and that serotonin release alone is required for this action. While ablation of sensory neurons does not limit bronchoconstriction, constriction after Ag challenge is absent in mice in which the cholinergic pathways are compromised. Linking mast cell function to the cholinergic system likely provides an important means of modulating the function of these resident immune cells to physiology of the lung, but may also provide a safeguard against life-threatening anaphylaxis during mast cell degranulation.


Assuntos
Antígenos/imunologia , Broncoconstrição/imunologia , Mastócitos/imunologia , Neurônios/imunologia , Anafilaxia/imunologia , Animais , Broncoconstrição/fisiologia , Mastócitos/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Serotonina/metabolismo , Vagotomia
4.
Am J Respir Cell Mol Biol ; 40(4): 433-42, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18931329

RESUMO

The multifunctional surface protein CD38 acts as a receptor with ecto-enzymatic activity, hydrolyzing NAD to generate several products known to exhibit Ca2+-mobilizing properties. Although CD38 is a convenient marker of immune cell development, and an indicator of progression for several diseases, it is not restricted to the immune compartment. To determine the potentially multilayered involvement of CD38 in allergen-induced airway inflammation and hyperreactivity, we dissected the potential role of CD38 as a regulator of immunity, but also pulmonary function. CD38-deficient and wild-type (WT) mice were sensitized and airway challenged with ovalbumin, and subsequently analyzed regarding their level of airway hyperresponsiveness (AHR) in response to methacholine. Parameters of lung inflammation were also analyzed. Similar sets of measurements were obtained from reciprocal bone marrow swapping experiments between CD38(-/-) and WT mice. Mice lacking CD38 exhibit strongly reduced AHR, which is accompanied by a decrease in typical hallmarks of pulmonary inflammation, including eosinophilia and lymphocytic lung infiltrates, as well as Th2-cytokine levels (IL-4, -5, and -13). Antigen-specific immunoglobulin (Ig)E and IgG1 antibody titers are substantially reduced, consistent with CD38 being crucial for mounting a primary humoral systemic immune response. Reconstitution of lethally irradiated, lung-shielded, CD38-deficient mice with WT bone marrow does not restore WT levels of airway hyperreactivity, nor mucus secretion. The opposite experiment, transferring CD38(-/-) bone marrow into WT mice, also shows reduced AHR levels. These studies demonstrate that CD38 not only acts as a key modulator of the immune response, but also plays an equally important role as an intrinsic pulmonary component.


Assuntos
ADP-Ribosil Ciclase 1/imunologia , Alérgenos/imunologia , Hipersensibilidade Respiratória/imunologia , ADP-Ribosil Ciclase 1/deficiência , Animais , Formação de Anticorpos , Medula Óssea/imunologia , Células Cultivadas , Quimera , Citocinas/biossíntese , Imunização , Imunoglobulina E/sangue , Imunoglobulina G/sangue , Chumbo , Pulmão/imunologia , Pulmão/patologia , Linfonodos/imunologia , Camundongos , Camundongos Endogâmicos C57BL , Ovalbumina/imunologia , Pneumonia/sangue , Pneumonia/imunologia , Pneumonia/patologia , Proteção Radiológica , Hipersensibilidade Respiratória/sangue , Hipersensibilidade Respiratória/patologia , Células Th2/imunologia
5.
Free Radic Biol Med ; 69: 348-56, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24512907

RESUMO

Extracellular superoxide dismutase (EC-SOD) is responsible for the dismutation of the superoxide radical produced in the extracellular space and known to be expressed by inflammatory cells, including macrophages and neutrophils. Here we show that EC-SOD is produced by resting macrophages and associated with the cell surface via the extracellular matrix (ECM)-binding region. Upon cellular activation induced by lipopolysaccharide, EC-SOD is relocated and detected both in the cell culture medium and in lipid raft structures. Although the secreted material presented a significantly reduced ligand-binding capacity, this could not be correlated to proteolytic removal of the ECM-binding region, because the integrity of the material recovered from the medium was comparable to that of the cell surface-associated protein. The naturally occurring R213G amino acid substitution located in the ECM-binding region of EC-SOD is known to affect the binding characteristics of the protein. However, the analysis of macrophages expressing R213G EC-SOD did not present evidence of an altered cellular distribution. Our results suggest that EC-SOD plays a dynamic role in the inflammatory response mounted by activated macrophages.


Assuntos
Inflamação/genética , Macrófagos/enzimologia , Neutrófilos/enzimologia , Superóxido Dismutase/genética , Substituição de Aminoácidos/genética , Animais , Matriz Extracelular/efeitos dos fármacos , Espaço Extracelular/enzimologia , Humanos , Inflamação/patologia , Lipopolissacarídeos/farmacologia , Macrófagos/ultraestrutura , Camundongos , Mutação , Neutrófilos/ultraestrutura , Ligação Proteica/efeitos dos fármacos , Superóxido Dismutase/química , Superóxido Dismutase/ultraestrutura , Superóxidos/metabolismo
6.
Circ Cardiovasc Genet ; 7(5): 659-66, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25085920

RESUMO

BACKGROUND: The enzyme extracellular superoxide dismutase (EC-SOD; SOD3) is a major antioxidant defense in lung and vasculature. A nonsynonomous single-nucleotide polymorphism in EC-SOD (rs1799895) leads to an arginine to glycine amino acid substitution at position 213 (R213G) in the heparin-binding domain. In recent human genetic association studies, this single-nucleotide polymorphism attenuates the risk of lung disease, yet paradoxically increases the risk of cardiovascular disease. METHODS AND RESULTS: Capitalizing on the complete sequence homology between human and mouse in the heparin-binding domain, we created an analogous R213G single-nucleotide polymorphism knockin mouse. The R213G single-nucleotide polymorphism did not change enzyme activity, but shifted the distribution of EC-SOD from lung and vascular tissue to extracellular fluid (eg, bronchoalveolar lavage fluid and plasma). This shift reduces susceptibility to lung disease (lipopolysaccharide-induced lung injury) and increases susceptibility to cardiopulmonary disease (chronic hypoxic pulmonary hypertension). CONCLUSIONS: We conclude that EC-SOD provides optimal protection when localized to the compartment subjected to extracellular oxidative stress: thus, the redistribution of EC-SOD from the lung and pulmonary circulation to the extracellular fluids is beneficial in alveolar lung disease but detrimental in pulmonary vascular disease. These findings account for the discrepant risk associated with R213G in humans with lung diseases compared with cardiovascular diseases.


Assuntos
Hipertensão Pulmonar/genética , Polimorfismo de Nucleotídeo Único , Superóxido Dismutase/genética , Animais , Antioxidantes/química , Arginina/química , Líquido da Lavagem Broncoalveolar , Predisposição Genética para Doença , Genótipo , Glicina/química , Heparina/química , Humanos , Pulmão/enzimologia , Pulmão/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Fatores de Risco , Sefarose/química , Análise de Sequência de DNA
7.
Methods Mol Biol ; 1032: 205-17, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23943455

RESUMO

This chapter provides an outline of the procedures necessary to measure airway hyperresponsiveness to inhaled methacholine in mouse models of allergic lung disease. We present a method for acquiring detailed measurements of respiratory mechanics using broadband low-frequency oscillatory waveforms applied at the subject's airway opening and analyzed using the constant phase model of the lung. We acknowledge that there are other methods of measuring airway responsiveness in allergic rodent models. However, a discussion of the merits and or detriments of these various methods have been vigorously debated in the primary literature and are beyond the scope of this chapter. The goal of this chapter is to provide a guide in how to begin these types of assays in laboratories which have little to no experience with these particular types of assessments.


Assuntos
Asma/induzido quimicamente , Hipersensibilidade/imunologia , Pulmão/imunologia , Cloreto de Metacolina/imunologia , Animais , Asma/imunologia , Modelos Animais de Doenças , Humanos , Hipersensibilidade/patologia , Pulmão/patologia , Camundongos , Mecânica Respiratória/imunologia
8.
Immunol Res ; 55(1-3): 162-6, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-22941563

RESUMO

Resident leukocytes in the lungs of healthy individuals are necessary for the innate and adaptive immune response toward potentially harmful foreign antigens that are inhaled on a constant basis. Under normal circumstances, inflammatory stimuli are effectively eradicated via innate immunity with accompanying resolution of inflammation and repair of the lung tissue. Work in our lab has explored how Arhgef1, an intracellular signaling molecule expressed by hematopoietic cells, participates in immune function with an emphasis on its contribution to pulmonary immunity. This review summarizes our studies on the role of Arhgef1 in regulating pulmonary macrophage function.


Assuntos
Fatores de Troca do Nucleotídeo Guanina/imunologia , Macrófagos Alveolares/imunologia , Animais , Fatores de Troca do Nucleotídeo Guanina/química , Humanos , Leucócitos/imunologia , Receptores de Tromboxanos/imunologia , Fatores de Troca de Nucleotídeo Guanina Rho
9.
Front Physiol ; 3: 300, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22934051

RESUMO

Exposure to second hand tobacco smoke is associated with the development and/or exacerbation of several different pulmonary diseases in humans. To better understand the possible effects of second hand smoke exposure in humans, we sub-chronically (4 weeks) exposed mice to a mixture of mainstream and sidestream tobacco smoke at concentrations similar to second hand smoke exposure in humans. The inflammatory response to smoke exposures was assessed at the end of this time by enumeration of pulmonary leukocyte infiltration together with measurements of lung elastance and pathology. This response was measured in both healthy wild type (C57BL/6) mice as well as mouse mutants deficient in the expression of Arhgef1 (Arhgef1(-/-)) that display constitutive pulmonary inflammation and decreased lung elastance reminiscent of emphysema. The results from this study show that sub-chronic second hand smoke exposure leads to significantly increased numbers of airspace leukocytes in both healthy and mutant animals. While sub-chronic cigarette smoke exposure is not sufficient to induce changes in lung architecture as measured by mean linear intercept, both groups exhibit a significant decrease in lung elastance. Together these data demonstrate that even sub-chronic exposure to second hand smoke is sufficient to induce pulmonary inflammation and decrease lung elastance in both healthy and diseased animals and in the absence of tissue destruction.

10.
Am J Physiol Lung Cell Mol Physiol ; 290(3): L526-33, 2006 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-16243899

RESUMO

Thromboxane A2 (TXA2) is a potent lipid mediator released by platelets and inflammatory cells and is capable of inducing vasoconstriction and bronchoconstriction. In the airways, it has been postulated that TXA2 causes airway constriction by direct activation of thromboxane prostanoid (TP) receptors on airway smooth muscle cells. Here we demonstrate that although TXA2 can mediate a dramatic increase in airway smooth muscle constriction and lung resistance, this response is largely dependent on vagal innervation of the airways and is highly sensitive to muscarinic acetylcholine receptor (mAChR) antagonists. Further analyses employing pharmacological and genetic strategies demonstrate that TP-dependent changes in lung resistance and airway smooth muscle tension require expression of the M2 mAChR subtype. These results raise the possibility that some of the beneficial actions of anticholinergic agents used in the treatment of asthma and chronic obstructive pulmonary disease result from limiting physiological changes mediated through the TP receptor. Furthermore, these findings demonstrate a unique pathway for TP regulation of homeostatic mechanisms in the airway and suggest a paradigm for the role of TXA2 in other organ systems.


Assuntos
Broncoconstrição/efeitos dos fármacos , Músculo Liso/efeitos dos fármacos , Receptor Muscarínico M3/metabolismo , Tromboxano A2/farmacologia , Resistência das Vias Respiratórias/efeitos dos fármacos , Animais , Feminino , Masculino , Camundongos , Camundongos Knockout , Contração Muscular , Músculo Liso/metabolismo , Receptores de Tromboxanos/metabolismo , Sistema Respiratório , Vagotomia
11.
Am J Physiol Lung Cell Mol Physiol ; 291(2): L144-56, 2006 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-16473862

RESUMO

Prostacyclin is one of a number of lipid mediators elaborated from the metabolism of arachidonic acid by the cyclooxygenase (COX) enzymes. This prostanoid is a potent inhibitor of platelet aggregation, and its production by endothelial cells and protective role in the vasculature are well established. In contrast, much less is known regarding the function of this prostanoid in other disease processes. We show here that COX-2-dependent production of prostacyclin plays an important role in the development of fibrotic lung disease, limiting both the development of fibrosis and the consequential alterations in lung mechanics. In stark contrast, loss of prostaglandin E(2) synthesis and signaling through the G(s)-coupled EP2 and EP4 receptors had no effect on the development of disease. These findings suggest that prostacyclin analogs will protect against bleomycin-induced pulmonary fibrosis in COX-2(-/-) mice. If such protection is observed, investigation of these agents as a novel therapeutic approach to pulmonary fibrosis in humans may be warranted.


Assuntos
Antibióticos Antineoplásicos/toxicidade , Bleomicina/toxicidade , Ciclo-Oxigenase 2/metabolismo , Epoprostenol/metabolismo , Fibrose Pulmonar , Animais , Ciclo-Oxigenase 1/genética , Ciclo-Oxigenase 1/metabolismo , Ciclo-Oxigenase 2/genética , Pulmão/citologia , Pulmão/patologia , Pulmão/fisiologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Prostaglandinas/metabolismo , Fibrose Pulmonar/induzido quimicamente , Fibrose Pulmonar/metabolismo
12.
Am J Physiol Lung Cell Mol Physiol ; 290(1): L105-13, 2006 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-16113047

RESUMO

Prostaglandin E2 (PGE2), similar to beta-adrenergic receptor agonists, can protect airways from bronchoconstriction and resulting increase in airway resistance induced by a number of agents, including cholinergic receptor agonists and antigen. We examined the impact of sustained alterations in PGE2 pathways on changes in airway resistance. Genetic methods were utilized to alter PGE2 metabolism and signal transduction in the murine lung. PGE2 levels were elevated by generating mice lacking 15-hydroxyprostaglandin (Hpgd-/-), the major catabolic enzyme of PGE2, and by generating a transgenic line in which mouse PGE2 synthase (Ptges) expression is driven by a human lung-specific promoter, hSP-C. Conversely, to determine the impact of loss of PGE2 on airway reactivity, we examined mice lacking this synthase (Ptges-/-) and receptors that mediate the actions of PGE2, particularly the PGE2 EP2 receptor (Ptger2). Diminished capacity to produce and respond to PGE2 did not alter the response of mice to cholinergic stimuli. In contrast, the responsiveness to cholinergic stimulation was dramatically altered in animals with elevated PGE2 levels. The Hpgd-/- and hSP-C-Ptges transgenic lines both showed attenuated airway responsiveness to methacholine as measured by lung resistance. Thus, whereas compromise of the Ptges/PGE2/Ptger2 pathway does not alter airway responsiveness, genetic modulation that elevates PGE2 levels in the lung attenuates airway responsiveness.


Assuntos
Resistência das Vias Respiratórias/fisiologia , Brônquios/metabolismo , Broncoconstrição/fisiologia , Dinoprostona/fisiologia , Animais , Brônquios/fisiologia , Humanos , Hidroxiprostaglandina Desidrogenases/deficiência , Oxirredutases Intramoleculares/deficiência , Oxirredutases Intramoleculares/genética , Oxirredutases Intramoleculares/metabolismo , Camundongos , Camundongos Endogâmicos , Camundongos Knockout , Camundongos Transgênicos , Regiões Promotoras Genéticas , Prostaglandina-E Sintases
13.
Am J Physiol Lung Cell Mol Physiol ; 284(4): L599-606, 2003 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-12618422

RESUMO

Prostaglandin E(2) (PGE(2)) has complex effects on airway tone, and the existence of four PGE(2) [E-prostanoid (EP)] receptors, each with distinct signaling characteristics, has provided a possible explanation for the seemingly contradictory actions of this lipid mediator. To identify the receptors mediating the actions of PGE(2) on bronchomotor tone, we examined its effects on the airways of wild-type and EP receptor-deficient mice. In conscious mice the administration of PGE(2) increased airway responsiveness primarily through the EP1 receptor, although on certain genetic backgrounds a contribution of the EP3 receptor was detected. These effects of PGE(2) were eliminated by pretreatment with either atropine or bupivacaine and were undetectable in anesthetized mice or in denervated tracheal rings, where only EP2-mediated relaxation of airway smooth muscle was observed. Together, our findings are consistent with a model in which PGE(2) modulates airway tone by activating multiple receptors expressed on various cell populations and in which the relative contribution of these receptors might depend on the expression of modifier alleles. PGE(2)/EP1/EP3-induced airway constriction occurs indirectly through activation of neural pathways, whereas PGE(2)-induced bronchodilation results from direct activation of EP2 receptors on airway smooth muscle. This segregation of EP receptor function within the airway suggests that PGE(2) analogs that selectively activate the EP2 receptor without activating the EP1/EP3 receptors might prove useful in the treatment of asthma.


Assuntos
Broncoconstrição/fisiologia , Dinoprostona/metabolismo , Receptores de Prostaglandina E/genética , Receptores de Prostaglandina E/metabolismo , Traqueia/fisiologia , Anestesia , Animais , Broncoconstrição/efeitos dos fármacos , Estado de Consciência , Dinoprostona/farmacologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos DBA , Camundongos Knockout , Músculo Liso/efeitos dos fármacos , Músculo Liso/fisiologia , Receptores de Prostaglandina E Subtipo EP1 , Receptores de Prostaglandina E Subtipo EP3 , Receptores de Prostaglandina E Subtipo EP4 , Traqueia/efeitos dos fármacos , Traqueia/inervação
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