[Effect of Intestinal Ischemia-reperfusion Injury on the Expression of Chemerin in Mice].

OBJECTIVE: To investigate the effect of intestinal ischemia-reperfusion on the protein expression of chemerin in C57BL/6J mice. METHODS: A total of 16 mice (C57BL/6J, specific pathogen free level) were randomly assigned into two groups (n=8 each): the intestinal ischemia-reperfusion group and the sham group. The intestinal ischemia-reperfusion state was achieved by blocking the super-mesenteric artery. After 60 minutes of ischemia followed by 60 minutes of reperfusion,we determined the protein level of chemerin in various organs and tissues by enzyme-linked immunosorbent assay and Western blot. The sham group underwent the same operation process except for the blocking of the super-mesenteric artery. RESULT: The protein level of chemerin was significantly elevated in distinctive organs and tissues in the state of intestinal ischemia-reperfusion (P<0.05). CONCLUSION: The intestinal ischemia-reperfusion can remarkably increase the protein expression of chemerin in some organs and tissues.

The effects of n-3 PUFA and intestinal lymph drainage on high-mobility group box 1 and Toll-like receptor 4 mRNA in rats with intestinal ischaemia-reperfusion injury.

The aim of the present study was to investigate the impacts of n-3 PUFA and lymph drainage (D) on intestinal ischaemia-reperfusion (I/R) injury in rats. A total of forty-eight Sprague-Dawley male rats were randomly divided into three groups (n 16): normal diet (N), enteral nutrition (EN) and EN plus n-3 PUFA. Each group was further divided into lymph drainage (I/R+D) and non-drainage (I/R) sub-groups (n 8). After 5 d with different nutrition regimens, the rats were subjected to 60 min ischaemia by clamping the superior mesenteric artery, followed by 120 min reperfusion. At the same time, the rats in the I/R+D sub-groups were treated with intestinal lymph drainage for 180 min. Organs were harvested and we detected the cytokine, endotoxin, and expression of Toll-like receptor (TLR) 4 mRNA and its endogenous ligand high-mobility group box 1 (HMGB1). We found that the serum levels of HMGB1, inflammatory cytokine and endotoxin in the three I/R+D sub-groups were significantly lower than those in the N (I/R) and EN (I/R) sub-groups (P < 0·05). The activation of NF-κB and the expression of HMGB1 and TLR4 mRNA significantly increased in the jejunum, ileum, liver and lung after intestinal I/R injury, but notably lower in the I/R+D groups than those in I/R (P < 0·05). The injury degree and HMGB1 expression were decreased in the n-3 PUFA group than in the N and EN groups. We preliminarily concluded that nutrition with n-3 PUFA and/or intestinal lymph drainage may reduce HMGB1 and inflammatory cytokine in serum and lymph and inhibit the expression and signal transmission of TLR4 mRNA, thereby alleviating intestinal I/R injury in rats.

[Estimating Methane Fugitive Emissions from Oil and Natural Gas Systems in China].

To achieve its carbon peaking and carbon neutrality objectives, China is committed to promoting a decarbonized energy transition, which has strengthened the shift from coal to oil and gas resources. As a result, methane (CH4) fugitive emissions from China's oil and gas systems are of increasing concern. Fugitive emissions include equipment leaks, venting, and flaring and involve exploration, production, transportation, storage, and distribution of oil and gas resources. However, there is no uniform accounting method for methane fugitive emissions from oil and gas systems, and fugitive emissions have not been included in the national greenhouse gas inventory statistics. Using the relevant methods, methane fugitive emissions from China's oil and gas systems were estimated for the period from 1980-2020. The results showed that CH4 fugitive emissions from oil and gas systems increased rapidly with the growth of production and consumption of oil and gas resources, from less than 0.6 million tons in 1980 to more than 2.6 million tons in 2020. CH4 fugitive emissions from oil and gas systems reached approximately 0.6 million tons and 2.0 million tons, which were 1.38 and 16.6 times larger than those in 1980, respectively. Fugitive emissions from oil and gas systems originated primarily from gas production, oil production, gas transportation, and storage, accounting for 41%, 20%, 18%, and 13% of total emissions, respectively. Gas pipelines were the main fugitive facilities. The emission intensity of unconventional oil and gas resource exploration was higher compared to conventional resource exploration. This study improved the CH4 fugitive emission inventory, which could provide solid scientific data for CH4 reduction.

[Effects of lymphatic drainage and omega-3 polyunsaturated fatty acids on intestinal ischemia-reperfusion injury in rats].

OBJECTIVE: To investigate the effects of lymphatic drainage and omega-3 polyunsaturated fatty acid (omega-3PUFA) on high mobility group box 1 (HMGB1), inflammatory cytokines and endotoxin in rats with intestinal ischemia-reperfusion (I/R) injury. METHODS: A total of 72 SD rats were randomly divided into drainage-alone group, I/R group, ischemia-reperfusion plus drainage (I/R + D) group (n = 8 each) and 3 groups with 16 rats undergoing gastrostomy in each group: normal diet (N) group, enteral nutrition (EN) group and enteral nutrition & omega-3PUFA (PUFA) group. And they were further divided into 2 subgroups (n = 8). The rats in I/R and I/R + D groups were subjected to a 60-min ischemia follow by 120-min reperfusion injury of superior mesenteric artery. When the rats suffered I/R injury, intestinal lymph was drained for 180 min in the I/R + D group. The rats in the drainage-alone group received 180-min lymph drainage without I/R injury. After 5 days with different nutrition regimes, the models were established similarly. The rats in the I/R + D sub-groups were treated with intestinal lymph drainage for 180 min. The serum and lymph samples were collected post-operatively. Endotoxin was detected by a Limulus kit. The inflammatory cytokines and high mobility group box 1 (HMGB1) were analyzed by enzyme-linked immunosorbent assay (ELISA). RESULTS: Endotoxin, inflammatory cytokines and lymphatic HMGB1 of lymphatic in the I/R + D group were higher than those in the drainage-alone group [all P < 0.05, IL-6: (30 +/- 8) pg/ml vs (20 +/- 6) pg/ml, endotoxin: (0.029 +/- 0.011) U/ml vs (0.008 +/- 0.005) U/ml]. The serum levels of endotoxin and inflammatory cytokines in the I/R + D group were lower than those in the I/R group (P < 0.05). The lymphatic levels of TNF-alpha (tumor necrosis factor-alpha) and HMGB1 in the N and EN groups were higher than those in the PUFA group[ TNF-alpha: (46 +/- 17) pg/ml, (54 +/- 16) pg/ml vs (28 +/- 9) pg/ml, HMGB1: (4.8 +/- 1.6) ng/ml, (5.3 +/- 1.8) ng/ml, (3.0 +/- 1.0) ng/ml, all P < 0.05)]. The serum levels of endotoxin, inflammatory cytokines and HMGB1 in the PUFA (I/R) group were lower than those in the N (I/R) group (P < 0.05). The levels of TNF-alpha and HMGB1 were lower in the PUFA (I/R + D) group than those in the N (I/R + D) group (both P < 0.05). CONCLUSION: Lymphatic drainage may reduce the levels of endotoxin, inflammatory cytokines and HMGB1 so as to alleviate the intestinal I/R injury. The intervention of omega-3PUFA has some protective effect through relieving inflammation.

[The sheltering effects of ω-3 polyunsaturated fatty acids and lymphatic drainage on distant organs after intestinal ischemia reperfusion injury in rats].

OBJECTIVE: To investigate the sheltering effects of ω-3 polyunsaturated fatty acid (ω-3PUFA) and lymphatic drainage on distant organs in intestinal ischemia-reperfusion injury in rats. METHODS: Forty-eight healthy Sprague-Dawley (SD) male rats (SPF grade) were randomly divided into 3 groups (16 rats in each group): normal diet group (N), enteral nutrition group (EN), enteral nutrition and ω-3PUFA group(PUFA group). Each group was divided into lymphatic drainage (I/R + D) group and no-drainage (I/R) group (n = 8). Each rats received gastrostomy. After given different nutrition for five days, the rats subjected to 60 min ischemia and 120 min reperfusion injury of the superior mesenteric artery. When the rats subjected to ischemia-reperfusion injury, drained intestinal lymph for 180 min in the I/R + D group. The serum level of alanine aminotransferase (ALT) and level of myeloperoxidase (MPO), nitric oxide (NO), total of nitric oxide synthase (tNOS), inducible nitric oxide synthase (iNOS) of lung were detected. The organ injury of lung and liver and the expression of high mobility group box 1(HMGB1, the endogenous ligand of TLR4) in these organs were investigated too. RESULTS: The serum level of ALT in PUFA I/R + D and I/R group and EN I/R + D group were significantly lower than that in normal diet I/R group: (46 ± 20), (53 ± 15), (46 ± 21) and (100 ± 60) U/L (P < 0.05), respectively. The level of MPO, NO, tNOS, iNOS in lung in the I/R + D group were significantly lower than those in I/R group (P < 0.05): MPO (0.73 ± 0.15):(0.85 ± 0.10) unit/grams wet slice; NO (0.72 ± 0.51):(1.79 ± 1.32) µmol/gprot; tNOS (0.46 ± 0.15):(0.78 ± 0.27) U/mgprot; iNOS (0.06 ± 0.04):(0.11 ± 0.07) U/mgprot, respectively. The level of tNOS in PUFA I/R group was significantly lower than that in normal diet I/R group: (0.56 ± 0.13):(0.78 ± 0.27) U/mgprot (P < 0.05). MPO, NO, INOS levels in PUFA group were reduced compared with those in EN and normal diet group. HE stained sections and HMGB1 immunohistochemistry results showed that the organ injury in I/R group was severer than that in I/R + D group. The expression of HMGB1 increased in I/R group. The organ injury and the expression of HMGB1 in PUFA group were less than that in the other two main groups. CONCLUSIONS: Lymphatic drainage can alleviate injury of distant organs after intestinal ischemia-reperfusion in rats. ω-3 polyunsaturated fatty acids can increase body resistance to injury and promote recovery.

[Integrated Chinese and Western medicine and clinical nutrition].

Nutritional support, an important measure for critical patients subject to monitoring, is widely used in clinical practice now. Nutrients have been used early in the Chinese medicine therapy. A number of Chinese medicine prescriptions show nutritional improvement and immune function enhancing effects on critical and/or postoperative patients, and some Chinese herbs are nutrient substances. Although the theoretical bases of Chinese medicine and Western medicine are different, they could work together in the clinical nutritional treatment to form a therapeutic measure with Chinese characteristics, which could promote the heritage and development of Chinese medicine. A discussion regarding the relationship between Chinese medicine drug-therapy, acupuncture and nutrition was also given.

[Method for drainage of lymph fluid and determining the change of active materials in lymph fluid after rat ischemia-reperfusion injury].

OBJECTIVE: To set up a method for the drainage of lymph fluid and explore the change of active materials in lymph fluid and serum after rat ischemia-reperfusion injury. METHODS: The method of the drainage of lymph fluid was well established. Sixteen healthy male rats of SPF grade were selected and randomly divided into 2 groups: intestinal ischemia-reperfusion + drainage group (I/R + drainage group) and drainage group. All the rats were subjected to superior mesenteric artery occlusion for 60 minutes, followed by 120 minutes of reperfusion. We compared the change of high mobility group box-1 (HMGB1) protein, endotoxin tumor necrosis factor alpha (TNF-alpha), interleukin (IL) -1 beta, IL-6, and soluble vascular cell adhesion molecular-1 (sICAM-1) by draining lymph fluid and collecting serum in 2 groups. RESULTS: The drainage of lymph fluid was successfully performed. The HMGB1, endotoxin, and cytokines in serum and lymph fluid were significantly higher in ischemia-reperfusion group than in drainage group (P < 0. 05). CONCLUSIONS: The method for drainage of lymph fluid is simple and feasible. Endotoxin, HMGB1, and some cytokines in serum and lymph fluid may mediate the ischemia-reperfusion injury.

[Analysis of maltose clearance in plasma and urine of healthy volunteers with high-performance liquid chromatography].

OBJECTIVE: To analyze the maltose clearance in plasma and urine of healthy volunteers with high-performance liquid chromatography. METHODS: Maltose solution was infused to 12 healthy volunteers during a 4-hour period at an infusion rate of 0.2, 0.3, and 0.5 g/(kg x h), Plasma and urine specimens were collected at different time points before and after infusion, and then analyzed with high-performance liquid chromatography. RESULTS: The coefficients of variation of the precision and accuracy of the analysis method ranged 3.68%-4.58% and 0.44%-4.83% for plasma, respectively, and 2.91%-7.62% and 0.95%-8.27% for urine, respectively. The plasma maltose concentration increased in a dose-dependent manner (r > 0.99). The plasma maltose concentrations returned to the baseline levels 12 hours later. Two hours after injection, the urinary excretion of maltose increased, reached the peak value within 2-4 hours, began to decrease 6 hours later, and became zero 24 hours later. CONCLUSIONS: An infusion rate of 0.2-0.5 g/(kg x h) of maltose will not remarkably increase the blood glucose level in healthy people. The routine infusion rate should below 0.3 g/(kg x h), unless an emergency exists.

[Noninvasive quantification of glutamine and glutamate in skeletal muscles of perioperative rabbits with proton magnetic resonance spectroscopy].

OBJECTIVE: To evaluate the feasibility of noninvasive quantification of the metabolic changes of glutamine (Gln) and glutamate (Glu) in the skeletal muscles caused by abdominal surgery with proton magnetic resonance spectroscopy (MRS). METHODS: Abdominal surgery were performed on 15 healthy Zelanian rabbits. MRS data, blood specimens, and muscular tissues were obtained on the day before surgery and the second day after surgery. MRS data included peak amplitude and peak area of Gln + Glu (Glx) and total creatine (TCr). The reproducibility of peak amplitude with peak area was compared and the better one was chosen to calculate the ratio of Glx to TCr. Free Gln and Glu concentrations in serum and muscles were analyzed by reversed phase high-performance liquid chromatography (RP-HPLC). The results of MRS were compared with those of RP-HPLC to evaluate the feasibility of MRS application in Gln and Glu metabolism. RESULTS: Peak amplitude had better reproducibility than peak area under our conditions. The coefficient of variation (CV) of the ratio of the amplitude of Glx to TCr was (15.62 +/- 9.87)%. The ratio of the amplitude of Glx to TCr significantly decreased on the second day after surgery (P <0.05, P <0.01). The mean free Glx concentrations in both the serum and the muscles different were not significant between on the second day after surgery and on the day before surgery. CONCLUSIONS: The results of MRS application in reflecting the effects of surgical trauma on muscular Glx metabolism are consistent with those of RP-HPLC. It is feasible to apply MRS in the study of muscular Gln and Glu metabolism.

Multi-indicator assessment of water environment in government environmental auditing.

This study focused on a multi-indicator assessment methodology for governmental environmental auditing of water protection programs. The environmental status of Wuli Lake in China was assessed using the global indicators (driver-status-response) developed by the Commission on Sustainable Development, and four additional indicators proposed by the author: water quality, pollution load, aquatic ecosystem status, and lake sediment deposition. Various hydrological, chemical, biological and environmental parameters were used to estimate the values of the indicators for assessment of environmental status of the lake based on time series data sets for twenty years. The indicators proposed can be customized to meeting the needs for particular assessment of water protection programs. This method can be used to evaluate the performance of national environmental protection programs and provide technical support for environmental auditors.

Identification of anthropogenic influences on water quality of rivers in Taihu watershed.

Surface water bodies are progressively subjected to stress as a result of anthropogenic activities. This study assessed and examined the impact of human activities on spatial variation in the water quality of 19 rivers in the Taihu watershed. Concentrations of physicochemical parameters of surface water quality were determined at the mouth of each river during the period of 2000-2004. Multivariate statistical techniques were applied to identify characteristics of the water quality in the studied rivers. The results showed that rivers strongly influenced by household wastewater have the highest concentrations of nutrients (TN and TP). Moreover, rivers in the vicinity of a metropolis presented low dissolved oxygen (DO) levels. However, organic-chemical pollution (petroleum and volatile phenolics) was identified with high localization. Two rivers influenced by sewage from industry and ships were distinguished from other rivers with high values of petroleum. The Taige channel, a river located in Changzhou City that is strongly influenced by wastewater from industry, was characterized with an extraordinarily high value of volatile phenolics. Rivers passing through countries, especially through hilly countries were characterized with high DO contents and low nutrient and organic-chemical pollution, suggesting that agriculture puts less pressure on water quality in adjacent rivers. Therefore, more effort should be made in controlling point pollution to restore water quality in rivers adjacent to cities.

Exploration of relationships between phytoplankton biomass and related environmental variables using multivariate statistic analysis in a eutrophic shallow lake: a 5-year study.

Understanding the process of the changing phytoplankton patterns can be particularly useful in water quality improvement and management decisions. However, it is generally not easy to illustrate the interactions between phytoplankton biomass and related environmental variables given their high spatial and temporal heterogeneity. To elucidate relationships between them, in a eutrophic shallow lake, Taihu Lake, relative long-term data set of biotic and abiotic parameters of water quality in the lake were conducted using multivariate statistical analysis within seasonal periodicity. The results indicate that water temperature and total phosphorus (TP) played governing roles in phytoplankton dynamics in most seasons (i.e. temperature in winter, spring and summer; TP in spring, summer and autumn); COD (chemical oxygen demand) and BOD (biological oxygen demand) presented significant positive relationships with phytoplankton biomass in spring, summer and autumn. However, a complex interplay was found between phytoplankton biomass and nitrogen considering significant positive relationships occurring between them in spring and autumn, and conversely negative ones in summer. As the predatory factor, zooplankton presented significant grazing-pressure on phytoplankton biomass during summer in view of negative relationship between them in the season. Significant feedback effects of phytoplankton development were identified in summer and autumn in view that significant relationships were observed between phytoplankton biomass and pH, Trans (transparency of water) and DO. The results indicate that interactions between phytoplankton biomass and related environmental variables are highly sensitive to seasonal periodicity, which improves understanding of different roles of biotic and abiotic variables upon phytoplankton variability, and hence, advances management methods for eutrophic lakes.

[Measurement of glutamine concentration in Zelanian rabbits' skeletal muscle by non-invasive magnetic resonance spectroscopy].

OBJECTIVE: To study the feasibility of non-invasive magnetic resonance spectroscopy (MRS) in measuring glutamine (GLN) level in Zelanian rabbits' skeletal muscle. METHODS: Non-invasive MRS was used to get the data of peak height ratio of GLN + glutamic acid (GLx) at 3.8 ppm and creatine (Cr) at 3.0 ppm, peak area ratio of GLx at 3.8 ppm and Cr at 3.0 ppm. High performance liquid chromatography (HPLC) was used to examine the actual GLx levels of muscle from 22 Zelanian rabbits. The feasibility of MRS was then evaluated by HPLC method. RESULTS: The ratio of peak height and peak area of GLx and Cr by means of MRS were 0.162 +/- 0.045 and 0.092 +/- 0.065, respectively. The average concentration of GLx in skeletal muscle by means of HPLC was (4.19 +/- 2.50) micromol/g. The ratio of GLx and plasma Cr level by means of HPLC was 4.576 -/+ 0.599. The ratio of peak height and peak area of GLx and Cr by means of MRS were correlated significantly with the ratio of concentration of GLx in skeletal muscle and plasma Cr by means of HPLC (r = 0.7, P = 0.001; r = 0.6, P = 0.001). CONCLUSION: Non-invasive MRS is feasible to measure GLN level in skeletal muscle of rabbit.

[Application of nutrigenomics in clinical nutrition].

In the past decade, the focus of nutritional study shifted from epidemiology and physiology to molecular biology. Advanced research strategies and technologies including genomics, transcriptomics, proteomics, metabolomics, and system biology have been gradually applied in clinical nutrition. This article reviews the effects of nutrients on gene expressions, application of modern molecular biology in clinical nutrition, as well as the advances and challenges in recent years..

[Single nucleotide polymorphisms, inflammation and nutrition of genes].

Inflammation is part of the immune response, and inflammation may also induce or exaggerate some diseases through production of pro-inflammatory cytokines. More evidence have shown that the individual level of cytokine production is affected by single nucleotide polymorphisms in cytokine genes. Furthermore, as several nutrients participate in DNA protection and stabilization, altering gene expression and individual phenotype, nutrition has important interaction with inflammation. The purpose of this review is to give a recent update informations on the interaction of single nucleotide polymorphisms, inflammation and nutrition.

TLR4-HMGB1-, MyD88- and TRIF-dependent signaling in mouse intestinal ischemia/reperfusion injury.

AIM: To characterize high-mobility group protein 1-toll-like receptor 4 (HMGB1-TLR4) and downstream signaling pathways in intestinal ischemia/reperfusion (I/R) injury. METHODS: Forty specific-pathogen-free male C57BL/6 mice were randomly divided into five groups (n = 8 per group): sham, control, anti-HMGB1, anti-myeloid differentiation gene 88 (MyD88), and anti-translocating-chain-associating membrane protein (TRIF) antibody groups. Vehicle with the control IgG antibody, anti-HMGB1, anti-MyD88, or anti-TRIF antibodies (all 1 mg/kg, 0.025%) were injected via the caudal vein 30 min prior to ischemia. After anesthetization, the abdominal wall was opened and the superior mesenteric artery was exposed, followed by 60 min mesenteric ischemia and then 60 min reperfusion. For the sham group, the abdominal wall was opened for 120 min without I/R. Levels of serum nuclear factor (NF)-κB p65, interleukin (IL)-6, and tumor necrosis factor (TNF)-α were measured, along with myeloperoxidase activity in the lung and liver. In addition,morphologic changes that occurred in the lung and intestinal tissues were evaluated. Levels of mRNA transcripts encoding HMGB1 and NF-κB were measured by real-time quantitative PCR, and levels of HMGB1 and NF-κB protein were measured by Western blot. Results were analyzed using one-way analysis of variance. RESULTS: Blocking HMGB1, MyD88, and TRIF expression by injecting anti-HMGB1, anti-MyD88, or anti-TRIF antibodies prior to ischemia reduced the levels of inflammatory cytokines in serum; NF-κB p65: 104.64 ± 11.89, 228.53 ± 24.85, 145.00 ± 33.63, 191.12 ± 13.22, and 183.73 ± 10.81 (P < 0.05); IL-6: 50.02 ± 6.33, 104.91 ± 31.18, 62.28 ± 6.73, 85.90 ± 17.37, and 78.14 ± 7.38 (P < 0.05); TNF-α, 43.79 ± 4.18, 70.81 ± 6.97, 52.76 ± 5.71, 63.19 ± 5.47, and 59.70 ± 4.63 (P < 0.05) for the sham, control, anti-HMGB1, anti-MyD88, and anti-TRIF groups, respectively (all in pg/mL).Antibodies also alleviated tissue injury in the lung and small intestine compared with the control group in the mouse intestinal I/R model. The administration of anti-HMGB1, anti-MyD88, and anti-TRIF antibodies markedly reduced damage caused by I/R, for which anti-HMGB1 antibody had the most obvious effect. CONCLUSION: HMGB1 and its downstream signaling pathway play important roles in the mouse intestinal I/R injury, and the effect of the TRIF-dependent pathway is slightly greater.

Omega-3 polyunsaturated fatty acids inhibit the increase in cytokines and chemotactic factors induced in vitro by lymph fluid from an intestinal ischemia-reperfusion injury model.

OBJECTIVE: To investigate the active factors and the intervention effect of ω-3 polyunsaturated fatty acids (PUFAs) during intestinal ischemia-reperfusion (I/R) injury, which causes the inflammation of monocytes-macrophages cultured in lymph fluid and stimulated with ω-3 PUFAs. METHODS: Forty-eight Sprague-Dawley male rats were randomly divided into the following two groups: A. (N + D) group and B. (I/R + D) group. The rats in the (N + D) group were drained of lymph for 180 min; the rats in the (I/R + D) group were subjected to 60 min ischemia by clamping the superior mesenteric artery followed by 120 min reperfusion and 180 min of lymph draining. Lymph fluid from each group was further divided into 4 subgroups, respectively: lymph group (A1, B1); eicosopentaenoic acid (EPA)-treated group (A2, B2); EPA + docosahexaeonic acid (DHA)-treated group (A3, B3); and DHA-treated group (A4, B4), then cultured monocyte-macrophage cell line. RESULTS: The levels of tumor necrosis factor-α, interleukin (IL)-1 ß, IL-6, soluble cell adhesion molecule-1, chemotactic factors macrophage chemoattractant protein-1, macrophage inflammatory protein-2, and high mobility group box protein 1 in the B1 group were significantly higher than in the A1 group. Importantly, addition of EPA, EPA + DHA, and DHA to the culture media significantly reduced the levels of the above-mentioned factors. Cell stimulation with EPA, EPA + DHA, and DHA also significantly decreased the expression of Toll-like receptor 4, nuclear factor-κB p65, macrophage chemoattractant protein-1, and macrophage inflammatory protein-2 with the combined treatment of EPA and DHA showing the strongest effect. CONCLUSIONS: The factors induced in lymph during intestinal I/R injury can cause inflammation in vitro. These data provide in vitro evidence that ω-3 PUFAs provide a protective effect by reducing the inflammatory response caused by intestinal I/R lymph. Moreover, the synergism of EPA and DHA had the greatest effect, which is possibly mediated through Toll-like receptor 4 and nuclear factor-κB p65.

[Construction of index system for early warning of persistent organic pollutants (POPs) pollution incidents in China].

Early warning of pollution incidents caused by persistent organic pollutants (POPs) is urgently needed for China in the circumstances of serious POPs pollution and in increasing demand for improvement in chemical risk management. Given different categories of POPs and pollution incidents, the index system for early warning of POPs pollution accidents was built based on lifecycle theory and POPs formation mechanisms. It will be helpful for decision makers to enhance the early warning management of POPs pollution incidents in China. The index system for early warning includes two parts, early warning and mechanism for system operation. The indices include risk source indicators, warning indicators and warning level indicators. To ensure the effective implementation of this system, the mechanisms for response and policy guarantee were also formulated. These mechanisms contain dynamic inventory management and periodical assessment of risk sources, timely and effective report of warning conditions, as well as coordination and cooperation among the relevant departments.

[Risk communication in construction of new nuclear power plant].

Accompanied by construction of new nuclear power plants in the coming decades in China, risk management has become increasingly politicized and contentious. Nuclear risk communication is a critical component in helping individuals prepare for, respond to, and recover from nuclear power emergencies. It was discussed that awareness of trust and public attitudes are important determinants in nuclear power risk communication and management. However, there is limited knowledge about how to best communicate with at-risk populations around nuclear power plant in China. To bridge this gap, this study presented the attitudinal data from a field survey in under-building Haiyang nuclear power plant, Shandong Province to measure public support for and opposition to the local construction of nuclear power plant. The paper discussed the structure of the communication process from a descriptive point of view, recognizing the importance of trust and understanding the information openness. The results showed that decision-making on nuclear power was dominated by a closed "iron nuclear triangle" of national governmental agencies, state-owned nuclear enterprises and scientific experts. Public participation and public access to information on nuclear constructions and assessments have been marginal and media was a key information source. As information on nuclear power and related risks is very restricted in China, Chinese citizens (51%) tend to choose the government as the most trustworthy source. More respondents took the negative attitudes toward nuclear power plant construction around home. It drew on studies about risk communication to develop some guidelines for successful risk communication. The conclusions have vast implications for how we approach risk management in the future. The findings should be of interest to state and local emergency managers, community-based organizations, public health researchers, and policy makers.

Impact of intestinal ischemia/reperfusion and lymph drainage on distant organs in rats.

AIM: To investigate the impact of intestinal ischemia/reperfusion (I/R) injury and lymph drainage on distant organs in rats. METHODS: Thirty-two Sprague-Dawley male rats, weighing 280-320 g, were randomly divided into blank, sham, I/R, and ischemia/reperfusion and drainage (I/R + D) groups (n = 8). All rats were subjected to 60 min ischemia by clamping the superior mesenteric artery, followed by 120 min reperfusion. The rats in the I/R + D group received intestinal lymph drainage for 180 min. In the sham group, the abdominal cavity was opened for 180 min, but the rats received no treatment. The blank group served as a normal and untreated control. A chromogenic limulus assay kit was used for quantitative detection of serum endotoxin. The serum concentrations of tumor necrosis factor-α (TNF-α), interleukin (IL)-6, IL-1ß, soluble cell adhesion molecules (sICAM-1), and high mobility group protein box 1 (HMGB1) were determined with an enzyme-linked immunosorbent assay kit. Histological evaluations of the intestine, liver, kidney, and lung were performed by hematoxylin and eosin staining and immunohistochemistry. HMGB1 protein expression was assayed by western blot analysis. RESULTS: The serum levels of endotoxin and HMGB1 in the I/R and I/R + D groups were significantly higher than those in the sham group (endotoxin, I/R and I/R + D vs sham: 0.033 ± 0.004 EU/mL, 0.024 ± 0.003 EU/mL vs 0.017 ± 0.009 EU/mL, respectively, P < 0.05; HMGB1, I/R and I/R + D vs sham: 5.473 ± 0.963 EU/mL, 4.906 ± 0.552 EU/mL vs 0.476 ± 0.406 EU/mL, respectively, P < 0.05). In addition, endotoxin and HMGB1 were significantly lower in the I/R + D group compared to the I/R group (P < 0.05). The serum inflammatory factors IL-6, IL-1ß, and sICAM-1 in the I/R and I/R + D groups were significantly higher than those in the sham group (IL-6, I/R and I/R + D vs sham: 41.773 ± 9.753 pg/mL, 19.204 ± 4.136 pg/mL vs 11.566 ± 2.973 pg/mL, respectively, P < 0.05; IL-1ß, I/R and I/R + D vs sham: 144.646 ± 29.378 pg/mL, 65.829 ± 10.888 pg/mL vs 38.178 ± 7.157 pg/mL, respectively, P < 0.05; sICAM-1, I/R and I/R + D vs sham: 97.360 ± 12.714 ng/mL, 48.401 ± 6.547 ng/mL vs 33.073 ± 5.957 ng/mL, respectively; P < 0.05). The serum TNF-α in the I/R group were significantly higher than in the sham group (45.863 ± 11.553 pg/mL vs 18.863 ± 6.679 pg/mL, respectively, P < 0.05). These factors were significantly lower in the I/R + D group compared to the I/R group (P < 0.05). The HMGB1 immunohistochemical staining results showed no staining or apparent injury in the blank group, and slight staining at the top of the microvillus was detected in the sham group. In the I/R group, both the top of villi and the basement membrane were stained for HMGB1 in most areas, and injury in the I/R + D group was less than that in the I/R group. HMGB1 expression in the liver, kidney, and lung of rats in the I/R + D group was significantly lower than the rats in the I/R group (P < 0.05). CONCLUSION: Lymph drainage could block the "gut-lymph" pathway, improve intestinal barrier function, and attenuate distant organ injury incurred by intestinal I/R.