Advancing understanding of Ficus carica: a comprehensive genomic analysis reveals evolutionary patterns and metabolic pathway insights.

Ficus carica L. (dioecious), the most significant commercial species in the genus Ficus, which has been cultivated for more than 11,000 years and was one of the first species to be domesticated. Herein, we reported the most comprehensive F. carica genome currently. The contig N50 of the Orphan fig was 9.78 Mb, and genome size was 366.34 Mb with 13 chromosomes. Based on the high-quality genome, we discovered that F. carica diverged from Ficus microcarpa ~34 MYA, and a WGD event took place about 2─3 MYA. Throughout the evolutionary history of F. carica, chromosomes 2, 8, and 10 had experienced chromosome recombination, while chromosome 3 saw a fusion and fission. It is worth proposing that the chromosome 9 experienced both inversion and translocation, which facilitated the emergence of the F. carica as a new species. And the selections of F. carica for the genes of recombination chromosomal fragment are compatible with their goal of domestication. In addition, we found that the F. carica has the FhAG2 gene, but there are structural deletions and positional jumps. This gene is thought to replace the one needed for female common type F. carica to be pollinated. Subsequently, we conducted genomic, transcriptomic, and metabolomic analysis to demonstrate significant differences in the expression of CHS among different varieties of F. carica. The CHS playing an important role in the anthocyanin metabolism pathway of F. carica. Moreover, the CHS gene of F. carica has a different evolutionary trend compared to other Ficus species. These high-quality genome assembly, transcriptomic, and metabolomic resources further enrich F. carica genomics and provide insights for studying the chromosomes evolution, sexual system, and color characteristics of Ficus.

Insights into the genetic determination of tuber shape and eye depth in potato natural population based on autotetraploid potato genome.

Potato is one of the world's most important food crops, with a time-consuming breeding process. In this study, we performed a genome-wide association (GWAS) analysis of the two important traits of potato tuber shape and eye depth, using the tetraploid potato genome (2n=4x=48) as a reference. A total of 370 potatoes were divided into three subgroups based on the principal component analysis and evolutionary tree analysis. The genetic diversity within subgroups is low (5.18×10-5, 4.36×10-5 and 4.24×10-5). Genome-wide linkage disequilibrium (LD) analysis showed that their LD is about 60 Kb. GWAS analysis identified that 146 significant single nucleotide polymorphism (SNP) loci at Chr01A1:34.44-35.25 Mb and Chr02A1:28.35-28.54 Mb regions are significantly associated with potato tuber shape, and that three candidate genes that might be related to potato tuber traits, PLATZ transcription factor, UTP-glucose-1-phosphate uridylyltransferase and FAR1 DNA-binding domain, are in the association region of Chr02A1. GWAS analysis identified 53 significant SNP loci at Chr05A2: 49.644-50.146 Mb and Chr06A2: 25.866-26.384 Mb regions with robust associations with potato tuber eye depth. Hydrolase and methyltransferases are present in the association region of Chr05A2, and three CYPs are present in the association region of Chr06A2. Our findings suggested that these genes are closely associated with potato tuber shape and eye depth. Our study identified molecular markers and candidate genes for improving tetraploid potato tuber shape and eye depth and provided ideas and insights for tetraploid potato breeding.

Polyaniline@magnetic chitosan nanomaterials for highly efficient simultaneous adsorption and in-situ chemical reduction of hexavalent chromium: Removal efficacy and mechanisms.

Devising new versatile nano-adsorbents for efficient capturing of heavy metals in water represents one important direction for environmental remediation. Here, the application of a novel polyaniline@magnetic chitosan (PANI@MCTS) composite consisting of numerous nitrogen-containing functional groups and magnetic Fe3O4 nanoparticles was reported for the efficient treatment of chromium-containing wastewater. This material exhibited a fast adsorption kinetics (80% removal efficiency within 15 min) and strong adsorption capacity (186.6 mg(Cr(VI))•g-1(PANI@MCTS)) for removing Cr(VI) in water as well as an excellent magnetic separation ability. The adsorption of Cr(VI) was found to follow the Langmuir isotherm model and comply with the pseudo-second-order kinetics. More importantly, the PANI@MCTS could facilitate the in-situ chemical reduction of Cr(VI) to Cr(III) that enabled the detoxification treatment of Cr(VI) in water. XPS analysis revealed the simultaneous adsorption and in-situ chemical reduction of Cr(VI) on the PANI@MCTS, where the coordination and electrostatic interaction between Cr(VI) and the positively charged nitrogen containing functional groups contributed to the adsorption, and the = N-/-NH- groups served as active redox pair triggered the in-situ chemical reduction reaction. The recycle experiment showed an excellent stability of this material with >90% removal efficiency after five repeats of treatment. This work provides a promising alternative material for the effective treatment of chromium-containing wastewater.