Component respiration, ecosystem respiration and net primary production of a mature black spruce forest in northern Quebec.

We measured respiratory fluxes of carbon dioxide by aboveground tree components and soil respiration with chambers in 2005 and scaled up these measurements over space and time to estimate annual ecosystem respiration (R(e)) at a mature black spruce (Picea mariana (Mill.) B.S.P.) ecosystem in Quebec, Canada. We estimated periodic annual net primary production (NPP) for this ecosystem also. R(e) was estimated at 10.32 Mg C ha(-)(1) year(-)(1); heterotrophic respiration (R(h)) accounted for 52% of R(e) and autotrophic respiration (R(a)) accounted for the remainder. We estimated NPP at 3.02 Mg C ha(-1) year(-1), including production of bryophyte biomass but not including shrub NPP. We used these estimates of carbon fluxes to calculate a carbon use efficiency [CUE = NPP/(NPP + R(a))] of 0.38. This estimate of CUE is similar to those reported for other boreal forest ecosystems and it is lower than the value frequently used in global studies. Based on the estimate of R(h) being greater than the estimate of NPP, the ecosystem was determined to emit approximately 2.38 Mg C ha(-1) year(-1) to the atmosphere in 2005. Estimates of gross primary production (GPP = NPP + R(a)) and R(e) differed substantially from estimates of these fluxes derived from eddy covariance measurements during 2005 at this site. The ecological estimates of GPP and R(e) were substantially greater than those estimated for eddy covariance measurements. Applying a correction for lack of energy balance closure to eddy covariance estimates reduces differences with ecological estimates. We reviewed possible sources of systematic error in ecological estimates and discuss other possible explanations for these discrepancies.

Leaf responsiveness of Populus tremula and Salix viminalis to soil contaminated with heavy metals and acidic rainwater.

Fast-growing trees such as Salix viminalis L. and Populus tremula L. are well suited to phytoremediate heavy metal contaminated soils. However, information on tree performance, particularly leaf function, under conditions of heavy metal contamination is scarce. We used yearly coppiced saplings of S. viminalis and P. tremula growing in model ecosytems to test four hypotheses: (1) heavy metal contamination impairs photosynthesis by injuring leaf structure; (2) the effects of heavy metal contamination are enhanced by acidified rainwater and low soil pH; (3) heavy metal contamination increases dark respiration and, thus, repair processes; and (4) heavy metal contamination is tolerated and remediated better by S. viminalis than by P. tremula. We investigated heavy metal accumulation, tissue injury and gas exchange in leaves of plants subjected to controlled soil contamination with heavy metal dust. Additional treatments included acidic and calcareous natural forest subsoils in combination with irrigation with rainwater at pH 5.5 or 3.5. In both provenances of P. tremula that were studied, but not in S. viminalis, heavy metal treatment reduced photosynthesis and transpiration by varying amounts, except in the hot and dry summer of 2003, but had no effect on dark respiration. At light saturation, net CO(2) uptake and water-use efficiency were reduced by heavy metal contamination, whereas the CO(2) concentration in the leaf intercellular air space was increased. Rainwater pH and subsoil pH only slightly modified the effects of the heavy metal treatment on P. tremula. Gas exchange responses of P. tremula to heavy metals were attributed to leaf structural and ultrastructural changes resulting from hypersensitive-response-like processes and accelerated mesophyll cell senescence and necroses in the lower epidermis, especially along the transport pathways of heavy metals in the leaf lamina. Overall, the effects of heavy metals on P. tremula corroborated Hypothesis 1, but refuted Hypotheses 2 and 3, and were inconclusive for Hypothesis 4. Both P. tremula and S. viminalis showed appreciable potential for storing heavy metals in aging foliage.

Effects of metal-contaminated soil on the performance of young trees growing in model ecosystems under field conditions.

Young Populus tremula, Salix viminalis, Betula pendula and Picea abies trees were grown together in large open-top chambers. The treatments were: without or with (Cu/Zn/Cd/Pb=640/3000/10/90 mg kg-1) metal contamination in the topsoil, irrigation pH 3.5 or 5.5, and acidic or calcareous subsoil. Growth, metal allocation to foliage and wood, as well as leaf gas exchange were measured. Biomass was reduced in P. tremula and B. pendula by the metal-contaminated topsoil relative to uncontaminated topsoil, whereas in P. tremula photosynthesis and transpiration were decreased. These effects were related to the elevated foliar Zn accumulation in P. tremula. S. viminalis showed a significant reduction in growth and an increased Zn and Cd accumulation on acidic vs. calcareous subsoil. Acidic irrigation produced only a few significant effects. P. abies showed the lowest metal uptake and no growth response to metal contamination.