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1.
J Neurochem ; 139(1): 106-19, 2016 10.
Artigo em Inglês | MEDLINE | ID: mdl-27379837

RESUMO

Prolonged metabolic suppression in the brain is a well-characterized secondary pathology of both experimental and clinical traumatic brain injury (TBI). AMP-activated kinase (AMPK) acts as a cellular energy sensor that, when activated, regulates various metabolic and catabolic pathways to decrease ATP consumption and increase ATP synthesis. As energy availability after TBI is suppressed, we questioned if increasing AMPK activity after TBI would improve cognitive outcome. TBI was delivered using the electromagnetic controlled cortical impact model on male Sprague-Dawley rats (275-300 g) and C57BL/6 mice (20-25 g). AMPK activity within the injured parietal cortex and ipsilateral hippocampus was inferred by western blots using phospho-specific antibodies. The consequences of acute manipulation of AMPK signaling on cognitive function were assessed using the Morris water maze task. We found that AMPK activity is decreased as a result of injury, as indicated by reduced AMPK phosphorylation and corresponding changes in the phosphorylation of its downstream targets: ribosomal protein S6 and Akt Substrate of 160 kDa (AS160). Increasing AMPK activity after injury using the drugs 5-amino-1-ß-d-ribofuranosyl-imidazole-4-carboxamide or metformin did not affect spatial learning, but significantly improved spatial memory. Taken together, our results suggest that decreased AMPK activity after TBI may contribute to the cellular energy crisis in the injured brain, and that AMPK activators may have therapeutic utility. Increased phosphorylation of Thr172 activates AMP-activated protein kinase (AMPK) under conditions of low cellular energy availability. This leads to inhibition of energy consuming, while activating energy generating, processes. Hill et al., present data to indicate that TBI decreases Thr172 phosphorylation and that its stimulation by pharmacological agents offers neuroprotection and improves memory. These results suggest that decreased AMPK phosphorylation after TBI incorrectly signals the injured brain that excess energy is available, thereby contributing to the cellular energy crisis and memory impairments.


Assuntos
Proteínas Quinases Ativadas por AMP/efeitos dos fármacos , Proteínas Quinases Ativadas por AMP/metabolismo , Lesões Encefálicas Traumáticas/metabolismo , Ativadores de Enzimas/farmacologia , Nootrópicos/farmacologia , Aminoimidazol Carboxamida/análogos & derivados , Aminoimidazol Carboxamida/farmacologia , Animais , Lesões Encefálicas Traumáticas/psicologia , Hipocampo/patologia , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Transtornos da Memória/etiologia , Transtornos da Memória/prevenção & controle , Transtornos da Memória/psicologia , Metformina/farmacologia , Camundongos , Camundongos Endogâmicos C57BL , Fármacos Neuroprotetores/farmacologia , Lobo Parietal/patologia , Fosforilação , Desempenho Psicomotor/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley , Ribonucleotídeos/farmacologia
2.
eNeuro ; 7(1)2020.
Artigo em Inglês | MEDLINE | ID: mdl-31941661

RESUMO

Brain-derived neurotrophic factor (BDNF) signals through its cognate receptor tropomyosin receptor kinase B (TrkB) to promote the function of several classes of inhibitory interneurons. We previously reported that loss of BDNF-TrkB signaling in cortistatin (Cort)-expressing interneurons leads to behavioral hyperactivity and spontaneous seizures in mice. We performed bulk RNA sequencing (RNA-seq) from the cortex of mice with disruption of BDNF-TrkB signaling in cortistatin interneurons, and identified differential expression of genes important for excitatory neuron function. Using translating ribosome affinity purification and RNA-seq, we define a molecular profile for Cort-expressing inhibitory neurons and subsequently compare the translatome of normal and TrkB-depleted Cort neurons, revealing alterations in calcium signaling and axon development. Several of the genes enriched in Cort neurons and differentially expressed in TrkB-depleted neurons are also implicated in autism and epilepsy. Our findings highlight TrkB-dependent molecular pathways as critical for the maturation of inhibitory interneurons and support the hypothesis that loss of BDNF signaling in Cort interneurons leads to altered excitatory/inhibitory balance.


Assuntos
Interneurônios , Neuropeptídeos , Animais , Fator Neurotrófico Derivado do Encéfalo/genética , Expressão Gênica , Glicoproteínas de Membrana , Camundongos , Camundongos Endogâmicos C57BL , Proteínas Tirosina Quinases , Receptor trkB
3.
Neuropsychopharmacology ; 44(13): 2239-2246, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31170726

RESUMO

Brain-derived neurotrophic factor (BDNF) signaling regulates synaptic plasticity in the hippocampus (HC) and prefrontal cortex (PFC), and has been extensively linked with fear memory expression in rodents. Notably, disrupting BDNF production from promoter IV-derived transcripts enhances fear expression in mice, and decreases fear-associated HC-PFC synchrony, suggesting that Bdnf transcription from promoter IV plays a key role in HC-PFC function during fear memory retrieval. To better understand how promoter IV-derived BDNF controls HC-PFC connectivity and fear expression, we generated a viral construct that selectively targets cells expressing promoter IV-derived Bdnf transcripts ("p4-cells") for tamoxifen-inducible Cre-mediated recombination (AAV8-p4Bdnf-ERT2CreERT2-PEST). Using this construct, we found that ventral hippocampal (vHC) p4-cells are recruited during fear expression, and that activation of these cells causes exaggerated fear expression that co-occurs with disrupted vHC-PFC synchrony in mice. Our data highlight how this novel construct can be used to interrogate genetically defined cell types that selectively contribute to BDNF-dependent behaviors.


Assuntos
Fator Neurotrófico Derivado do Encéfalo/fisiologia , Medo/fisiologia , Hipocampo/fisiologia , Neurônios/fisiologia , Córtex Pré-Frontal/fisiologia , Animais , Fator Neurotrófico Derivado do Encéfalo/genética , Condicionamento Clássico , Sincronização Cortical , Hipocampo/metabolismo , Masculino , Camundongos Endogâmicos C57BL , Vias Neurais/fisiologia , Plasticidade Neuronal , Córtex Pré-Frontal/metabolismo
4.
Brain Struct Funct ; 224(1): 471-483, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30377803

RESUMO

Signaling of brain-derived neurotrophic factor (BDNF) via tropomyosin receptor kinase B (TrkB) plays a critical role in the maturation of cortical inhibition and controls expression of inhibitory interneuron markers, including the neuropeptide cortistatin (CST). CST is expressed exclusively in a subset of cortical and hippocampal GABAergic interneurons, where it has anticonvulsant effects and controls sleep slow-wave activity (SWA). We hypothesized that CST-expressing interneurons play a critical role in regulating excitatory/inhibitory balance, and that BDNF, signaling through TrkB receptors on CST-expressing interneurons, is required for this function. Ablation of CST-expressing cells caused generalized seizures and premature death during early postnatal development, demonstrating a critical role for these cells in providing inhibition. Mice in which TrkB was selectively deleted from CST-expressing interneurons were hyperactive, slept less and developed spontaneous seizures. Frequencies of spontaneous excitatory post-synaptic currents (sEPSCs) on CST-expressing interneurons were attenuated in these mice. These data suggest that BDNF, signaling through TrkB receptors on CST-expressing cells, promotes excitatory drive onto these cells. Loss of excitatory drive onto CST-expressing cells that lack TrkB receptors may contribute to observed hyperexcitability and epileptogenesis.


Assuntos
Comportamento Animal , Ondas Encefálicas , Fator Neurotrófico Derivado do Encéfalo/metabolismo , Encéfalo/metabolismo , Hipercinese/metabolismo , Interneurônios/metabolismo , Locomoção , Glicoproteínas de Membrana/metabolismo , Neuropeptídeos/metabolismo , Proteínas Tirosina Quinases/metabolismo , Convulsões/metabolismo , Transmissão Sináptica , Animais , Encéfalo/fisiopatologia , Potenciais Pós-Sinápticos Excitadores , Hipercinese/fisiopatologia , Hipercinese/prevenção & controle , Hipercinese/psicologia , Glicoproteínas de Membrana/deficiência , Glicoproteínas de Membrana/genética , Camundongos Endogâmicos C57BL , Camundongos Knockout , Inibição Neural , Neuropeptídeos/deficiência , Neuropeptídeos/genética , Proteínas Tirosina Quinases/deficiência , Proteínas Tirosina Quinases/genética , Convulsões/fisiopatologia , Convulsões/prevenção & controle , Convulsões/psicologia , Sono
5.
Curr Opin Neurobiol ; 36: 59-65, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26485574

RESUMO

Fear regulation is impaired in anxiety and trauma-related disorders. Patients experience heightened fear expression and reduced ability to extinguish fear memories. Because fear regulation is abnormal in these disorders and extinction recapitulates current treatment strategies, understanding the underlying mechanisms is vital for developing new treatments. This is critical because although extinction-based exposure therapy is a mainstay of treatment, relapse is common. We examine recent findings describing changes in network activity and functional connectivity within limbic circuits during fear regulation, and explore how activity-dependent signaling contributes to the neural activity patterns that control fear and anxiety. We review the role of the prototypical activity-dependent molecule, brain-derived neurotrophic factor (BDNF), whose signaling has been critically linked to regulation of fear behavior.


Assuntos
Ansiedade/fisiopatologia , Fator Neurotrófico Derivado do Encéfalo/fisiologia , Encéfalo/fisiopatologia , Condicionamento Clássico/fisiologia , Transtornos de Estresse Pós-Traumáticos/fisiopatologia , Animais , Ansiedade/metabolismo , Encéfalo/metabolismo , Fator Neurotrófico Derivado do Encéfalo/metabolismo , Medo/fisiologia , Humanos , Vias Neurais/fisiopatologia , Plasticidade Neuronal/fisiologia , Transtornos de Estresse Pós-Traumáticos/metabolismo
6.
Neuropsychopharmacology ; 41(8): 1943-55, 2016 07.
Artigo em Inglês | MEDLINE | ID: mdl-26585288

RESUMO

Brain-derived neurotrophic factor (BDNF) regulates diverse biological functions ranging from neuronal survival and differentiation during development to synaptic plasticity and cognitive behavior in the adult. BDNF disruption in both rodents and humans is associated with neurobehavioral alterations and psychiatric disorders. A unique feature of Bdnf transcription is regulation by nine individual promoters, which drive expression of variants that encode an identical protein. It is hypothesized that this unique genomic structure may provide flexibility that allows different factors to regulate BDNF signaling in distinct cell types and circuits. This has led to the suggestion that isoforms may regulate specific BDNF-dependent functions; however, little scientific support for this idea exists. We generated four novel mutant mouse lines in which BDNF production from one of the four major promoters (I, II, IV, or VI) is selectively disrupted (Bdnf-e1, -e2, -e4, and -e6 mice) and used a comprehensive comparator approach to determine whether different Bdnf transcripts are associated with specific BDNF-dependent molecular, cellular, and behavioral phenotypes. Bdnf-e1 and -e2 mutant males displayed heightened aggression accompanied by convergent expression changes in specific genes associated with serotonin signaling. In contrast, BDNF-e4 and -e6 mutants were not aggressive but displayed impairments associated with GABAergic gene expression. Moreover, quantifications of BDNF protein in the hypothalamus, prefrontal cortex, and hippocampus revealed that individual Bdnf transcripts make differential, region-specific contributions to total BDNF levels. The results highlight the biological significance of alternative Bdnf transcripts and provide evidence that individual isoforms serve distinct molecular and behavioral functions.


Assuntos
Agressão , Fator Neurotrófico Derivado do Encéfalo/genética , Regiões Promotoras Genéticas , Serotonina/metabolismo , Transdução de Sinais , Animais , Encéfalo/metabolismo , Regulação da Expressão Gênica , Hipocampo/metabolismo , Hipotálamo/metabolismo , Camundongos , Camundongos Transgênicos , Neurônios/metabolismo , Córtex Pré-Frontal/metabolismo , RNA Mensageiro/metabolismo , Ácido gama-Aminobutírico/metabolismo
7.
J Neurotrauma ; 32(2): 149-58, 2015 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-25025304

RESUMO

Although the mechanisms that contribute to the development of traumatic brain injury (TBI)-related deficits are not fully understood, it has been proposed that altered energy utilization may be a contributing factor. The tuberous sclerosis complex, a heterodimer composed of hamartin/Tsc-1 and tuberin/Tsc-2, is a critical regulatory node that integrates nutritional and growth signals to govern energy using processes by regulating the activity of mechanistic Target of Rapamycin complex 1 (mTORC1). mTORC1 activation results in enhanced protein synthesis, an energy consuming process. We show that mice that have a heterozygous deletion of Tsc2 exhibit elevated basal mTORC1 activity in the cortex and the hippocampus while still exhibiting normal motor and neurocognitive functions. In addition, a mild closed head injury (mCHI) that did not activate mTORC1 in wild-type mice resulted in a further increase in mTORC1 activity in Tsc2(+/KO) mice above the level of activity observed in uninjured Tsc2(+/KO) mice. This enhanced level of increased mTORC1 activity was associated with worsened cognitive function as assessed using the Morris water maze and context discrimination tasks. These results suggest that there is a threshold of increased mTORC1 activity after a TBI that is detrimental to neurobehavioral performance, and interventions to inhibit excessive mTORC1 activation may be beneficial to neurocognitive outcome.


Assuntos
Lesões Encefálicas/metabolismo , Transtornos Cognitivos/metabolismo , Cognição/fisiologia , Complexos Multiproteicos/metabolismo , Transdução de Sinais/genética , Serina-Treonina Quinases TOR/metabolismo , Animais , Lesões Encefálicas/complicações , Transtornos Cognitivos/etiologia , Alvo Mecanístico do Complexo 1 de Rapamicina , Camundongos , Camundongos Transgênicos , Complexos Multiproteicos/genética , Prognóstico , Serina-Treonina Quinases TOR/genética
8.
J Neurotrauma ; 30(9): 716-26, 2013 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-23489238

RESUMO

Concussive force can cause neurocognitive and neurobehavioral dysfunction by inducing functional, electrophysiological, and/or ultrastructural changes within the brain. Although concussion-triggered symptoms typically subside within days to weeks in most people, in 15%-20% of the cases, symptomology can continue beyond this time point. Problems with memory, attention, processing speed, and cognitive flexibility (e.g., problem solving, conflict resolution) are some of the prominent post-concussive cognitive symptoms. Repeated concussions (with loss or altered consciousness), which are common to many contact sports, can exacerbate these symptoms. The pathophysiology of repeated concussions is not well understood, nor is an effective treatment available. In order to facilitate drug discovery to treat post-concussive symptoms (PCSs), there is a need to determine if animal models of repeated mild closed head injury (mCHI) can mimic the neurocognitive and histopathological consequences of repeated concussions. To this end, we employed a controlled cortical impact (CCI) device to deliver a mCHI directly to the skull of mice daily for 4 days, and examined the ensuing neurological and neurocognitive functions using beam balance, foot-fault, an abbreviated Morris water maze test, context discrimination, and active place avoidance tasks. Repeated mCHI exacerbated vestibulomotor, motor, short-term memory and conflict learning impairments as compared to a single mCHI. Learning and memory impairments were still observed in repeated mCHI mice when tested 3 months post-injury. Repeated mCHI also reduced cerebral perfusion, prolonged the inflammatory response, and in some animals, caused hippocampal neuronal loss. Our results show that repeated mCHI can reproduce some of the deficits seen after repeated concussions in humans and may be suitable for drug discovery studies and translational research.


Assuntos
Concussão Encefálica/complicações , Concussão Encefálica/patologia , Concussão Encefálica/fisiopatologia , Transtornos da Memória/etiologia , Animais , Encéfalo/irrigação sanguínea , Encéfalo/patologia , Imuno-Histoquímica , Aprendizagem/fisiologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Destreza Motora/fisiologia , Recidiva
9.
Curr Opin Psychiatry ; 23(4): 304-10, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20502331

RESUMO

PURPOSE OF REVIEW: Treatment of children who develop schizophrenia in childhood and early adolescence presents unique considerations. There has been increasing attention to the importance of early intervention and whether treatment effects may be affected by brain development. RECENT FINDINGS: Several recent trials support the use of antipsychotics for treatment of schizophrenia in children and adolescents. Clozapine shows greater efficacy in children and adolescents than it has in adults. A large-scale trial comparing a first-generation antipsychotic (molindone) with newer agents did not find significant differences in treatment response, although the newer antipsychotics were associated with more severe weight gain. Data regarding effects of antipsychotics on brain development in children and young adolescents with schizophrenia are sparse, although one report found no difference between effects of clozapine and olanzapine on cortical thickness. SUMMARY: Although psychosocial interventions are an important adjunctive treatment, antipsychotic medications continue to be the mainstay of treatment. Careful monitoring of metabolic side effects and age-appropriate intervention is particularly important, as children and adolescents appear to be more likely to develop metabolic abnormalities such as pronounced weight gain, which may significantly impact adherence as well as lead to other health issues.


Assuntos
Antipsicóticos/uso terapêutico , Esquizofrenia/terapia , Adolescente , Criança , Humanos , Psicoterapia , Resultado do Tratamento
10.
Vision Res ; 50(20): 2080-92, 2010 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-20673778

RESUMO

Many studies have shown that the direction of gaze of a face covertly facilitates the response to a target presented in the matching direction. In this study we seek to determine whether there exist separate reflexive and voluntary forms of such covert social orienting and how they interact with each other. We measured the effect of the predictive value of a gaze cue on manual choice reaction times. When the predictive value of the gaze cue was zero, a facilitatory cueing effect was still observed which peaked at a cue onset to target onset delay (CTD) of 150ms and largely diminished beyond a CTD of 500ms. When the gaze cue was 100% predictive of the future location of the target, at CTDs greater than 200, the predictive cue resulted in a significantly greater facilitation of response than occurred with a non-predictive cue. These results suggest that given enough time (about 200ms), the social cue is interpreted and a willful or voluntary spatially-specific social cueing effect occurs. In addition, we found that a predictive cue resulted in a significant slowing of the observer's responses up to a CTD of 200ms. These findings show that, similar to non-social spatial orienting, there appear to be two forms of social orienting including a reflexive component and voluntary component. We suggest a model of social orienting in which the voluntary social orienting system modulates tonic inhibition of the reflexive social orienting system.


Assuntos
Atenção/fisiologia , Sinais (Psicologia) , Fixação Ocular/fisiologia , Reflexo/fisiologia , Comportamento de Escolha/fisiologia , Movimentos Oculares/fisiologia , Feminino , Humanos , Masculino , Valor Preditivo dos Testes , Tempo de Reação , Comportamento Social
11.
Reproduction ; 130(4): 517-28, 2005 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-16183869

RESUMO

Cumulus cell-oocyte complexes (COCs), cultured in vitro, are competent for maturation and fertilization. Inclusion of epidermal growth factor (EGF) in the COC culture medium enhances in vitro maturation and subsequent embryonic development. It has been shown that isolated COCs exposed to EGF respond with a prolonged and pulsatile release of Ca2+ into the extra-cellular medium and that cumulus cells (CCs) of complexes exhibit both a slow rise in intracellular [Ca2+] ([Ca2+]i) and plasma membrane permeabilization in response to EGF. These unusual signaling responses were examined in isolated, cultured bovine CCs. Few individual CCs showed [Ca2+]i increases; the lack of response was found to be due to decrease of expression of endogenous EGF receptors after dissociation. CCs transfected with a human EGF receptor-GFP fusion protein showed robust, prolonged, EGF-stimulated [Ca2+]i elevations characteristic of CC responses in intact COCs. Many CCs that responded to EGF stimulation with a [Ca2+]i rise also released entrapped fura-2 dye at the peak of the [Ca2+]i response, suggesting that CC permeabilization and death follows activation of the EGF receptor. The [Ca2+]i elevation due to EGF stimulation and subsequent membrane permeabilization was shown to be mediated by the inositol triphosphate signaling pathway.


Assuntos
Sinalização do Cálcio , Fator de Crescimento Epidérmico/farmacologia , Receptores ErbB/metabolismo , Oócitos/metabolismo , Animais , Western Blotting/métodos , Cálcio/metabolismo , Bovinos , Linhagem Celular Tumoral , Membrana Celular/metabolismo , Técnicas de Cocultura , Regulação para Baixo , Fator de Crescimento Epidérmico/metabolismo , Receptores ErbB/análise , Receptores ErbB/genética , Feminino , Imunofluorescência , Fura-2 , Proteínas de Fluorescência Verde/genética , Humanos , Fosfatos de Inositol/metabolismo , Reação em Cadeia da Polimerase/métodos , RNA Mensageiro/análise , Estimulação Química , Transfecção/métodos
12.
Reproduction ; 127(2): 207-20, 2004 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-15056787

RESUMO

The role of epidermal growth factor (EGF) in the maturation of mammalian oocytes is well known but not well characterized. It is known that EGF enhances oocyte maturation in vitro and that EGF stimulation of cumulus-oocyte complexes (COCs) induces pulsatile Ca(2+) efflux from the cell complex. By use of quantitative Fura-2 imaging, EGF-stimulated changes in intracellular [Ca(2+)] in germinal vesicle stage murine COCs are shown to occur in a subpopulation of cumulus cells that interact cooperatively within individual COCs. Oocytes fail to respond to EGF stimulus. In many of the cumulus cells responding with a rise in intracellular [Ca(2+)], a concomitant permeabilization of the plasma membrane is found. Neither cumulus cells of control COCs nor those that show a rise in intracellular [Ca(2+)] in response to calcium ionophore treatment display a similar membrane permeabilization, although those cells responding with a prolonged [Ca(2+)] increase in response to thimerosal or thapsigargin do display plasma membrane permeabilization. Thus, EGF stimulation of mammalian COCs activates release of Ca(2+) from intracellular stores of cumulus cells, the depletion of which activates permeabilization of the plasma membrane. This membrane permeabilization leads to loss of cell contents and presumptive cumulus cell death. This catastrophic EGF-induced plasma membrane permeabilization of individual cumulus cells within a COC leads to pulsatile Ca(2+) efflux as previously seen, and may lead to improved cumulus cell expansion during COC maturation.


Assuntos
Cálcio/metabolismo , Citosol/metabolismo , Fator de Crescimento Epidérmico/farmacologia , Oócitos/metabolismo , Oogênese/efeitos dos fármacos , Animais , Canais de Cálcio/metabolismo , Permeabilidade da Membrana Celular/efeitos dos fármacos , Células Cultivadas , Feminino , Corantes Fluorescentes/metabolismo , Fura-2/metabolismo , Receptores de Inositol 1,4,5-Trifosfato , Camundongos , Camundongos Endogâmicos , Microscopia de Fluorescência , Oócitos/efeitos dos fármacos , Receptores Citoplasmáticos e Nucleares/metabolismo , Estimulação Química
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