RESUMO
HLA DQ A1 is probably one of the PCR-based genetic marker systems most widely used in actual forensic casework analyses. As accurate data about the distribution of the alleles is one of the most important prerequisites for the application in forensic biology, we studied the allele distribution in two relevant Spanish populations (Aragonese and Asturian). Results were in good agreement with the Hardy-Weinberg law in both Aragonese and Asturian populations. The power of discrimination was 0.92 in the Aragonese and 0.93 in Asturian sample. A test for homogeneity of the HLA DQ A1 population data based on alelle frequency counts for 12 European samples was performed and no significant differences were found (P = 0.831).
Assuntos
Alelos , Frequência do Gene , Antígenos HLA-DQ/genética , Análise Discriminante , Medicina Legal , Marcadores Genéticos , Genótipo , Cadeias alfa de HLA-DQ , Humanos , Reação em Cadeia da Polimerase , Reprodutibilidade dos Testes , EspanhaRESUMO
In order to use genetic loci in forensic identity testing, some population data are needed. This paper presents a report of allele frequency data for the loci HUMCSF1PO, HUMF13A01, HUMFES/FPS and D12S391 in a population sample from Asturias (northern Spain). No deviation from the Hardy-Weinberg equilibrium was detected in any of the four markers investigated and there was no evidence of association between the alleles of these loci. Statistical analysis was also carried out to obtain some parameters of medico-legal interest and comparative studies were carried out with other populations studied to date for these loci. The Asturian sample does not differ substantially from other Caucasian and Spanish populations.
Assuntos
Impressões Digitais de DNA/métodos , Frequência do Gene/genética , Repetições Minissatélites/genética , Análise Discriminante , Triagem de Portadores Genéticos , Genótipo , Humanos , Polimorfismo Genético/genética , Estudos de Amostragem , EspanhaRESUMO
Allele and genotype frequencies of the loci LDLR, GYPA, HBGG, D7S8 and GC (PM loci) were investigated in a population sample of 215 unrelated individuals from Asturias (North Spain). Multiplex amplification and simultaneous typing of the five loci was carried out using the polymarker PCR amplification and typing kit. All loci met Hardy-Weinberg expectations. The Asturian sample does not differ significantly from other Caucasians, but significant differences were observed between this population and SW Hispanic, Afro-american and Korean populations.