Towards rapid colorimetric detection of extracellular vesicles using optofluidics-enhanced color-changing optical metasurface.

Efficient transportation and delivery of analytes to the surface of optical sensors are crucial for overcoming limitations in diffusion-limited transport and analyte sensing. In this study, we propose a novel approach that combines metasurface optics with optofluidics-enabled active transport of extracellular vesicles (EVs). By leveraging this combination, we show that we can rapidly capture EVs and detect their adsorption through a color change generated by a specially designed optical metasurface that produces structural colors. Our results demonstrate that the integration of optofluidics and metasurface optics enables spectrometer-less and label-free colorimetric read-out for EV concentrations as low as 107 EVs/ml, achieved within a short incubation time of two minutes.

Anapole-Assisted Low-Power Optical Trapping of Nanoscale Extracellular Vesicles and Particles.

This study addresses the challenge of trapping nanoscale biological particles using optical tweezers without the photothermal heating effect and the limitation presented by the diffraction limit. Optical tweezers are effective for trapping microscopic biological objects but not for nanoscale specimens due to the diffraction limit. To overcome this, we present an approach that uses optical anapole states in all-dielectric nanoantenna systems on distributed Bragg reflector substrates to generate strong optical gradient force and potential on nanoscale biological objects with negligible temperature rise below 1 K. The anapole antenna condenses the accessible electromagnetic energy to scales as small as 30 nm. Using this approach, we successfully trapped nanosized extracellular vesicles and supermeres (approximately 25 nm in size) using low laser power of only 10.8 mW. This nanoscale optical trapping platform has great potential for single molecule analysis while precluding photothermal degradation.

Multiplexed Long-Range Electrohydrodynamic Transport and Nano-Optical Trapping with Cascaded Bowtie Photonic Crystal Nanobeams.

Photonic crystal cavities with bowtie defects that combine ultrahigh Q and ultralow mode volume are theoretically studied for low-power nanoscale optical trapping. By harnessing the localized heating of the water layer near the bowtie region, combined with an applied alternating current electric field, this system provides long-range electrohydrodynamic transport of particles with average radial velocities of 30 µm/s towards the bowtie region on demand by switching the input wavelength. Once transported to a given bowtie region, synergistic interaction of optical gradient and attractive negative thermophoretic forces stably trap a 10 nm quantum dot in a potential well with a depth of 10 k_{B}T using a mW input power.

Engineering Electromagnetic Field Distribution and Resonance Quality Factor Using Slotted Quasi-BIC Metasurfaces.

Dielectric metasurfaces governed by bound states in the continuum (BIC) are actively investigated for achieving high-quality factors and strong electromagnetic field enhancements. Traditional approaches reported for tuning the performance of quasi-BIC metasurfaces include tuning the resonator size, period, and structure symmetry. Here we propose and experimentally demonstrate an alternative approach through engineering slots within a zigzag array of elliptical silicon resonators. Through analytical theory, three-dimensional electromagnetic modeling, and infrared spectroscopy, we systematically investigate the spectral responses and field distributions of the slotted metasurface in the mid-IR. Our results show that by introducing slots, the electric field intensity enhancement near the apex and the quality factor of the quasi-BIC resonance are increased by a factor of 2.1 and 3.3, respectively, in comparison to the metasurface without slots. Furthermore, the slotted metasurface also provides extra regions of electromagnetic enhancement and confinement, which holds enormous potential in particle trapping, sensing, and emission enhancement.

On-chip integrated quantum emitter with 'trap-enhance-guide': a simulation approach.

To address the challenges of developing a scalable system of an on-chip integrated quantum emitter, we propose to leverage the loss in our hybrid plasmonic-photonic structure to simultaneously achieve Purcell enhancement as well as on-chip maneuvering of nanoscale emitter via optical trapping with guided excitation-emission routes. In this report, we have analyzed the feasibility of the functional goals of our proposed system in the metric of trapping strength (∼8KBT), Purcell factor (>1000∼), and collection efficiency (∼10%). Once realized, the scopes of the proposed device can be advanced to develop a scalable platform for integrated quantum technology.

Electrothermoplasmonic Trapping and Dynamic Manipulation of Single Colloidal Nanodiamond.

Low-power trapping of nanoscale objects can be achieved by using the enhanced fields near plasmonic nanoantennas. Unfortunately, in this approach the trap site is limited to the position of the plasmonic hotspots and continuous dynamic manipulation is not feasible. Here, we report a low-frequency electrothermoplasmonic tweezer (LFET) that provides low-power, high-stability and continuous dynamic manipulation of a single nanodiamond. LFET harnesses the combined action of the laser illumination of a plasmonic nanopillar antenna array and low-frequency alternating current (ac) electric field to establish an electrohydrodynamic potential capable of the stable trapping and dynamic manipulation of single nanodiamonds. We experimentally demonstrate the fast transport, trapping, and dynamic manipulation of a single nanodiamond using a low-frequency ac field below 5 kHz and low-laser power of 1 mW. This nanotweezer platform for nanodiamond manipulation holds promise for the scalable assembly of single photon sources for quantum information processing and low noise quantum sensors.

Flat metaform near-eye visor.

A near-eye visor is one of the most vital components in a head-mounted display. Currently, freeform optics and waveguides are used to design near-eye visors, but these structures are complex and their field of view is limited when the visor is placed near the eye. In this paper, we propose a flat, freeform near-eye visor that uses a subwavelength patterned metasurface reflector. The visor design imparts a spatial phase profile on a projected display pattern and can be implemented using a micron-scale-thick metasurface. As the resulting metaform visor relies on diffraction, it can preserve a large field of view (77.3° both horizontally and vertically) when placed only 2.5 cm away from the eye. We simulate the metasurface visor to estimate the modulation transfer function, and find that the projected image quality is sufficiently high for human vision. While the design of the metasurface is initially performed via ray optics, using full-wave finite-difference time-domain simulation we validate a scaled version of our visor design.

Plasmonic dielectric antennas for hybrid optical nanotweezing and optothermoelectric manipulation of single nanosized extracellular vesicles.

This paper showcases an experimental demonstration of near-field optical trapping and dynamic manipulation of an individual extracellular vesicle. This is accomplished through the utilization of a plasmonic dielectric nanoantenna designed to support an optical anapole state-a non-radiating optical state resulting from the destructive interference between electric and toroidal dipoles in the far-field, leading to robust near-field enhancement. To further enhance the field intensity associated with the optical anapole state, a plasmonic mirror is incorporated, thereby boosting trapping capabilities. In addition to demonstrating near-field optical trapping, the study achieves dynamic manipulation of extracellular vesicles by harnessing the thermoelectric effect. This effect is induced in the presence of an ionic surfactant, cetyltrimethylammonium chloride (CTAC), combined with plasmonic heating. Furthermore, the thermoelectric effect improves trapping stability by introducing a wide and deep trapping potential. In summary, our hybrid plasmonic-dielectric trapping platform offers a versatile approach for actively transporting, stably trapping, and dynamically manipulating individual extracellular vesicles.

Scalable trapping of single nanosized extracellular vesicles using plasmonics.

Heterogeneous nanoscale extracellular vesicles (EVs) are of significant interest for disease detection, monitoring, and therapeutics. However, trapping these nano-sized EVs using optical tweezers has been challenging due to their small size. Plasmon-enhanced optical trapping offers a solution. Nevertheless, existing plasmonic tweezers have limited throughput and can take tens of minutes for trapping for low particle concentrations. Here, we present an innovative approach called geometry-induced electrohydrodynamic tweezers (GET) that overcomes these limitations. GET generates multiple electrohydrodynamic potentials, allowing parallel transport and trapping of single EVs within seconds. By integrating nanoscale plasmonic cavities at the center of each GET trap, single EVs can be placed near plasmonic cavities, enabling instant plasmon-enhanced optical trapping upon laser illumination without detrimental heating effects. These non-invasive scalable hybrid nanotweezers open new horizons for high-throughput tether-free plasmon-enhanced single EV trapping and spectroscopy. Other potential areas of impact include nanoplastics characterization, and scalable hybrid integration for quantum photonics.

High-speed nanoscale optical trapping with plasmonic double nanohole aperture.

Optical trapping with plasmonic double nanohole (DNH) apertures has proven to be an efficient method for trapping sub-50 nm particles due to their suppressed plasmonic heating effect and very high electric field enhancement in the gap region of the aperture. However, plasmonic tweezers are generally diffusion-limited, requiring particles to diffuse down to a few tens of nanometres from the high field enhancement regions before they can be trapped. The loading of target particles to the plasmonic hotspots can take several minutes for diluted samples. In this work, rapid particle transport and trapping of a 25 nm polystyrene sphere is demonstrated, leveraging an electrothermoplasmonic flow induced upon application of an AC field in the presence of a laser-induced temperature gradient. Using this approach, we demonstrate the rapid transport of a 25 nm polystyrene particle across a distance of 63 µm and trapping at the DNH under 16 s. This platform shows great potential for applications involving simultaneous trapping and plasmon-enhanced spectroscopies, such as Raman enhancement via the intense electric field enhancement in the DNH gap.

Exosomes trapping, manipulation and size-based separation using opto-thermo-electrohydrodynamic tweezers.

Owing to the heterogeneity of exosomes in size and biomolecular composition, there is a need for new approaches for trapping, manipulating, and sorting of single exosomes in solution. Due to their small size ranging from 30 nm to 150 nm and their relatively low refractive index, their stable trapping using optical tweezers has been met with challenges. Trapping exosomes in an optical trap requires nearly 100 mW of input power, which predisposes them to photo-induced damage and membrane rupture at the laser focus. Here, we report a high stability opto-thermo-electrohydrodynamic tweezer for the stable stand-off trapping of single exosomes based on a concentric nanohole array (CNA) using laser illumination and an a.c. field. The CNA system generates two regions of electrohydrodynamic potentials several microns away from the laser focus where single exosomes are trapped. We demonstrate the rapid trapping within seconds, and selective dynamic manipulation of exosomes based on size using only 4.2 mW of input laser power. The proposed platform opens up a promising approach for stabilizing single exosomes in solution and controlling their distribution based on size without the risk of photo-induced damage.

Stand-off trapping and manipulation of sub-10 nm objects and biomolecules using opto-thermo-electrohydrodynamic tweezers.

Optical tweezers have emerged as a powerful tool for the non-invasive trapping and manipulation of colloidal particles and biological cells1,2. However, the diffraction limit precludes the low-power trapping of nanometre-scale objects. Substantially increasing the laser power can provide enough trapping potential depth to trap nanoscale objects. Unfortunately, the substantial optical intensity required causes photo-toxicity and thermal stress in the trapped biological specimens3. Low-power near-field nano-optical tweezers comprising plasmonic nanoantennas and photonic crystal cavities have been explored for stable nanoscale object trapping4-13. However, the demonstrated approaches still require that the object is trapped at the high-light-intensity region. We report a new kind of optically controlled nanotweezers, called opto-thermo-electrohydrodynamic tweezers, that enable the trapping and dynamic manipulation of nanometre-scale objects at locations that are several micrometres away from the high-intensity laser focus. At the trapping locations, the nanoscale objects experience both negligible photothermal heating and light intensity. Opto-thermo-electrohydrodynamic tweezers employ a finite array of plasmonic nanoholes illuminated with light and an applied a.c. electric field to create the spatially varying electrohydrodynamic potential that can rapidly trap sub-10 nm biomolecules at femtomolar concentrations on demand. This non-invasive optical nanotweezing approach is expected to open new opportunities in nanoscience and life science by offering an unprecedented level of control of nano-sized objects, including photo-sensitive biological molecules.

Publisher Correction: Stand-off trapping and manipulation of sub-10 nm objects and biomolecules using opto-thermo-electrohydrodynamic tweezers.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.